Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0004325.1

Orthogroup ID

OG0002625

Physical Location & Seq

Genomic Coordinates Forward (+)

6366 .. 13092

Gene Structure 6727 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

atgacctctttggagacttcctgggaggtttttaggaaagtcctgttggcggcggctagtgacgggaatcaggcgaaagttggccagaatccgacgaccggtgacggggttccggcgaagtctcctatggtggtggtagaagtggtggcgcctaggcggcttgatctgggttgtggtcgatggtggatgttggacagcttaggcttctatcttgctgggctggctagcgacatagtgtcaatcgtgatggacgctcagatcatggatatttcggagcaccatctcctcaaccccaaccctgatttcgtcgccgacctctacacccgcatcctcatcgctctcgacttcttcaaccaggattttgggcaagttgatttcgatgccttgcagcagtttgagaatcccgattttcatgagacctccctccacagcatcaacctctgcaacagaatcaagcaagtcctcgccttagtcgattgcccttacaatttcaccctcaaagatctcctcagacccgaccccaatcgcaccgagtattttctcagtgctattatcaattaccatctttacagagagacgaaggtgaatctactcaccgaacttatgaacccgttaacggagattgaggagcagcaccaattgctgcagggccagatttcacagttgaatgctgagattgcgggtttcaatgaagcaagagaaaaggagttgcctcttcttcaagaggtagatgccagagttagagaattgcgccaaaccattgaaggccttaacaacgaccaagttaaagtcagaacttctcttaagaagttgaaggagaagagtggagaaatggacaaacagatttctgatgctgattttgatctgctaaaatgtgattccgagagacaagaattacgttcaagaattgtacagtcaccagataaactgcagagggctgtagaagagaagaaatcaattcaagagaaggcaaagaatgatgaaatgtcagccatgcaatcttttaaggagaagactgctgttgaggaggtttatacgaaggttctaaagaaattatcaaagcacttggcccagatgcaagttatacaagaacaggtcaattctgctaaatttgctggcaaagactataaagcattaaaagataaaataagtgatgatgaaatcctgattaagtctcttcagaacgaagttgtgaaacgacaggcaaaagtgaaagatttgaatgacttgaaaaggaaactagaaaaggaaagagatctaaagtttgaggaggctactaaagaatatagtaatgtcaagctggaggtggaatcaaggaggggtgttcttgaagcaaggcaaaaggatgttgaagctgcagtagcagaggtggattctataactgcaaaggttgcctcggtaagaaaatctgcagcagccgatcagcaagcattagctcataagtgtgaggagatcattaaagagttccaccaatatgcaagttcaattggggtcttgctgcaatga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000070 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005773 GO:0005774 GO:0005815 GO:0005816 GO:0005856 GO:0005886 GO:0006996 GO:0007049 GO:0007059 GO:0007080 GO:0007127 GO:0008150 GO:0008608 GO:0009987 GO:0015630 GO:0016020 GO:0016043 GO:0022402 GO:0022414 GO:0031090 GO:0031262 GO:0031617 GO:0031974 GO:0031981 GO:0032991 GO:0035974 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044437 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045143 GO:0048285 GO:0050000 GO:0051179 GO:0051234 GO:0051276 GO:0051303 GO:0051310 GO:0051315 GO:0051316 GO:0051321 GO:0051455 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0061982 GO:0070013 GO:0071840 GO:0071944 GO:0098588 GO:0098687 GO:0098805 GO:0098813 GO:0140013 GO:0140014 GO:1903046 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nuf2

Protein Analysis

501

Amino Acids

57.01

Weight (kDa)

5.48

Isoelectric Point (pI)

35.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nuf2	PF03800	76 - 203	1.7e-25	Nuf2 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002625)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G61000
fragaria_vesca	FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_6g25020 FvH4_7g06682 FvH4_7g06682 FvH4_7g06682 FvH4_7g06682 FvH4_7g06682
malus_domestica	MD08G1232500.v1.1 MD08G1233000.v1.1 MD15G1425300.v1.1
prunus_persica	Prupe.1G570900_v2.0.a1 Prupe.1G570900_v2.0.a1
pyrus_communis	pycom08g20190 pycom15g37700
rosa_chinensis	RchiOBHm_Chr1g0336301 RchiOBHm_Chr7g0222021
rosa_laevigata	RLG00000002102 RLG00000029374
rosa_multiflora	Rmu_co8043290.1_g000001 Rmu_sc0001453.1_g000009 Rmu_sc0004325.1_g000003
rosa_roxburghii	Rroxscaffold_3G00237490 Rroxscaffold_4G00315800
rosa_rugosa	Rorug01G0123600 Rorug07G0205100
rosa_samantha	Rh1AG144300 Rh1BG113700 Rh1CG135800 Rh1DG150800 Rh7AG345600 Rh7BG336200 Rh7CG363300 Rh7DG343900
rosa_wichuraiana	Rw0G015220 Rw7G029310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	148
AciI	CCGC	5 cut(s)	50, 53, 157, 325, 680
AcoI	YGGCCR	1 cut(s)	82
AcsI	RAATTY	1 cut(s)	1109
AcuI	CTGAAG	1 cut(s)	1163
AcyI	GRCGYC	1 cut(s)	149
AfaI	GTAC	1 cut(s)	912
AfiI	CCNNNNNNNGG	4 cut(s)	49, 98, 105, 616
AflII	CTTAAG	1 cut(s)	803
AgeI	ACCGGT	1 cut(s)	98
AhdI	GACNNNNNGTC	1 cut(s)	235
AjnI	CCWGG	2 cut(s)	20, 354
AjuI	GAANNNNNNNTTGG	4 cut(s)	29, 61, 287, 319
AluBI	AGCT	4 cut(s)	198, 1300, 1355, 1436
AluI	AGCT	4 cut(s)	198, 1300, 1355, 1436
Alw21I	GWGCWC	1 cut(s)	279
Alw26I	GTCTC	6 cut(s)	8, 126, 410, 569, 880, 1179
AoxI	GGCC	4 cut(s)	82, 649, 766, 1068
ApeKI	GCWGC	7 cut(s)	388, 631, 643, 1355, 1412, 1415, 1498
ApoI	RAATTY	1 cut(s)	1109
AsiGI	ACCGGT	1 cut(s)	98
AspA2I	CCTAGG	1 cut(s)	152
AspLEI	GCGC	2 cut(s)	151, 753
AspS9I	GGNCC	2 cut(s)	649, 1069
AsuHPI	GGTGA	5 cut(s)	113, 484, 586, 595, 909
AsuNHI	GCTAGC	1 cut(s)	224
AvrII	CCTAGG	1 cut(s)	152
BalI	TGGCCA	1 cut(s)	84
BanI	GGYRCC	1 cut(s)	148
BbsI	GAAGAC	1 cut(s)	1019
Bbv12I	GWGCWC	1 cut(s)	279
BbvI	GCAGC	7 cut(s)	400, 630, 643, 1342, 1424, 1427, 1485
BccI	CCATC	4 cut(s)	174, 241, 288, 570
BcgI	CGANNNNNNTGC	2 cut(s)	316, 350
BciT130I	CCWGG	2 cut(s)	22, 356
BcoDI	GTCTC	6 cut(s)	8, 126, 410, 569, 880, 1179
BfaI	CTAG	4 cut(s)	57, 153, 225, 1241
BfmI	CTRYAG	5 cut(s)	644, 929, 939, 1356, 1410
BfoI	RGCGCY	1 cut(s)	152
BfrI	CTTAAG	1 cut(s)	803
BglI	GCCNNNNNGGC	1 cut(s)	157
BglII	AGATCT	2 cut(s)	502, 1255
BlnI	CCTAGG	1 cut(s)	152
Bme1390I	CCNGG	2 cut(s)	22, 356
BmeRI	GACNNNNNGTC	1 cut(s)	235
BmgT120I	GGNCC	2 cut(s)	649, 1069
BmiI	GGNNCC	2 cut(s)	111, 150
BmrFI	CCNGG	2 cut(s)	22, 356
BmsI	GCATC	6 cut(s)	336, 370, 441, 721, 841, 1065
BmtI	GCTAGC	1 cut(s)	228
BpiI	GAAGAC	1 cut(s)	1019
BpmI	CTGGAG	1 cut(s)	1322
Bpu10I	CCTNAGC	1 cut(s)	199
BsaBI	GATNNNNATC	1 cut(s)	861
BsaHI	GRCGYC	1 cut(s)	149
BsaI	GGTCTC	1 cut(s)	410
BsaJI	CCNNGG	3 cut(s)	21, 152, 1395
BsaWI	WCCGGW	1 cut(s)	98
Bsc4I	CCNNNNNNNGG	4 cut(s)	49, 98, 105, 616
Bse118I	RCCGGY	1 cut(s)	98
Bse8I	GATNNNNATC	1 cut(s)	861
BseBI	CCWGG	2 cut(s)	22, 356
BseDI	CCNNGG	3 cut(s)	21, 152, 1395
BseGI	GGATG	3 cut(s)	192, 327, 1351
BseJI	GATNNNNATC	1 cut(s)	861
BseLI	CCNNNNNNNGG	4 cut(s)	49, 98, 105, 616
BseMII	CTCAG	4 cut(s)	269, 523, 556, 663
BseRI	GAGGAG	6 cut(s)	275, 497, 641, 1040, 1283, 1463
BseXI	GCAGC	7 cut(s)	400, 630, 643, 1342, 1424, 1427, 1485
BseYI	CCCAGC	1 cut(s)	215
Bsh1285I	CGRYCG	1 cut(s)	99
BshFI	GGCC	4 cut(s)	84, 651, 768, 1070
BshNI	GGYRCC	1 cut(s)	148
BshTI	ACCGGT	1 cut(s)	98
BsiEI	CGRYCG	1 cut(s)	99
BsiHKAI	GWGCWC	1 cut(s)	279
BsiSI	CCGG	2 cut(s)	99, 113
BslI	CCNNNNNNNGG	4 cut(s)	49, 98, 105, 616
BsmAI	GTCTC	6 cut(s)	8, 126, 410, 569, 880, 1179
BsmBI	CGTCTC	1 cut(s)	569
BsmI	GAATGC	1 cut(s)	673
BsnI	GGCC	4 cut(s)	84, 651, 768, 1070
Bso31I	GGTCTC	1 cut(s)	410
Bsp1286I	GDGCHC	1 cut(s)	279
Bsp1407I	TGTACA	1 cut(s)	910
Bsp143I	GATC	7 cut(s)	163, 258, 502, 862, 1255, 1420, 1452
BspACI	CCGC	5 cut(s)	50, 53, 157, 325, 680
BspANI	GGCC	4 cut(s)	84, 651, 768, 1070
BspCNI	CTCAG	4 cut(s)	268, 522, 555, 664
BspHI	TCATGA	1 cut(s)	412
BspLI	GGNNCC	2 cut(s)	111, 150
BspMAI	CTGCAG	4 cut(s)	648, 933, 1360, 1414
BspOI	GCTAGC	1 cut(s)	228
BspT107I	GGYRCC	1 cut(s)	148
BspTI	CTTAAG	1 cut(s)	803
BspTNI	GGTCTC	1 cut(s)	410
BsrFI	RCCGGY	1 cut(s)	98
BsrGI	TGTACA	1 cut(s)	910
BssAI	RCCGGY	1 cut(s)	98
BssECI	CCNNGG	3 cut(s)	21, 152, 1395
BssMI	GATC	7 cut(s)	163, 258, 502, 862, 1255, 1420, 1452
BssNI	GRCGYC	1 cut(s)	149
BssT1I	CCWWGG	1 cut(s)	152
Bst2UI	CCWGG	2 cut(s)	22, 356
Bst4CI	ACNGT	2 cut(s)	663, 915
Bst6I	CTCTTC	4 cut(s)	720, 815, 939, 1182
BstACI	GRCGYC	1 cut(s)	149
BstAFI	CTTAAG	1 cut(s)	803
BstAPI	GCANNNNNTGC	2 cut(s)	640, 1391
BstAUI	TGTACA	1 cut(s)	910
BstC8I	GCNNGC	4 cut(s)	222, 226, 1117, 1428
BstDEI	CTNAG	6 cut(s)	199, 255, 469, 509, 542, 672
BstF5I	GGATG	3 cut(s)	192, 327, 1351
BstH2I	RGCGCY	1 cut(s)	152
BstHHI	GCGC	2 cut(s)	151, 753
BstKTI	GATC	7 cut(s)	166, 261, 505, 865, 1258, 1423, 1455
BstMAI	GTCTC	6 cut(s)	8, 126, 410, 569, 880, 1179
BstMBI	GATC	7 cut(s)	163, 258, 502, 862, 1255, 1420, 1452
BstMCI	CGRYCG	1 cut(s)	99
BstMWI	GCNNNNNNNGC	7 cut(s)	157, 221, 640, 677, 1112, 1361, 1391
BstNI	CCWGG	2 cut(s)	22, 356
BstSCI	CCNGG	2 cut(s)	20, 354
BstSFI	CTRYAG	5 cut(s)	644, 929, 939, 1356, 1410
BstV1I	GCAGC	7 cut(s)	400, 630, 643, 1342, 1424, 1427, 1485
BstV2I	GAAGAC	1 cut(s)	1019
BstX2I	RGATCY	2 cut(s)	502, 1255
BstXI	CCANNNNNNTGG	1 cut(s)	362
BstYI	RGATCY	2 cut(s)	502, 1255
BsuRI	GGCC	4 cut(s)	84, 651, 768, 1070
BtgZI	GCGATG	1 cut(s)	319
BtsCI	GGATG	3 cut(s)	192, 327, 1351
BtsIMutI	CAGTG	1 cut(s)	550
Cac8I	GCNNGC	4 cut(s)	222, 226, 1117, 1428
CciI	TCATGA	1 cut(s)	412
CfoI	GCGC	2 cut(s)	151, 753
Cfr10I	RCCGGY	1 cut(s)	98
Cfr13I	GGNCC	2 cut(s)	649, 1069
CseI	GACGC	1 cut(s)	260
Csp6I	GTAC	1 cut(s)	911
CspAI	ACCGGT	1 cut(s)	98
CviAII	CATG	3 cut(s)	262, 413, 994
CviQI	GTAC	1 cut(s)	911
DdeI	CTNAG	6 cut(s)	199, 255, 469, 509, 542, 672
DinI	GGCGCC	1 cut(s)	150
DpnI	GATC	7 cut(s)	165, 260, 504, 864, 1257, 1422, 1454
DpnII	GATC	7 cut(s)	163, 258, 502, 862, 1255, 1420, 1452
DriI	GACNNNNNGTC	1 cut(s)	235
EaeI	YGGCCR	1 cut(s)	82
Eam1104I	CTCTTC	4 cut(s)	720, 815, 939, 1182
Eam1105I	GACNNNNNGTC	1 cut(s)	235
EarI	CTCTTC	4 cut(s)	720, 815, 939, 1182
Eco130I	CCWWGG	1 cut(s)	152
Eco147I	AGGCCT	1 cut(s)	768
Eco31I	GGTCTC	1 cut(s)	410
Eco57I	CTGAAG	1 cut(s)	1163
EcoRII	CCWGG	2 cut(s)	20, 354
EcoT14I	CCWWGG	1 cut(s)	152
EgeI	GGCGCC	1 cut(s)	150
EheI	GGCGCC	1 cut(s)	150
ErhI	CCWWGG	1 cut(s)	152
Esp3I	CGTCTC	1 cut(s)	569
FaeI	CATG	3 cut(s)	265, 416, 997
FatI	CATG	3 cut(s)	261, 412, 993
FauI	CCCGC	2 cut(s)	332, 673
FokI	GGATG	3 cut(s)	199, 314, 1358
FspBI	CTAG	4 cut(s)	57, 153, 225, 1241
GlaI	GCGC	2 cut(s)	150, 752
GsaI	CCCAGC	1 cut(s)	219
GsuI	CTGGAG	1 cut(s)	1322
HaeII	RGCGCY	1 cut(s)	152
HaeIII	GGCC	4 cut(s)	84, 651, 768, 1070
HapII	CCGG	2 cut(s)	99, 113
HgaI	GACGC	1 cut(s)	260
HhaI	GCGC	2 cut(s)	151, 753
Hin1I	GRCGYC	1 cut(s)	149
Hin1II	CATG	3 cut(s)	265, 416, 997
Hin6I	GCGC	2 cut(s)	149, 751
HinP1I	GCGC	2 cut(s)	149, 751
HincII	GTYRAC	1 cut(s)	615
HindII	GTYRAC	1 cut(s)	615
HinfI	GANTC	8 cut(s)	67, 88, 400, 450, 586, 878, 1310, 1373
HpaI	GTTAAC	1 cut(s)	615
HpaII	CCGG	2 cut(s)	99, 113
HphI	GGTGA	5 cut(s)	113, 484, 586, 595, 909
Hpy166II	GTNNAC	1 cut(s)	615
Hpy188I	TCNGA	8 cut(s)	93, 258, 274, 512, 794, 850, 883, 1182
Hpy188III	TCNNGA	9 cut(s)	244, 341, 404, 413, 722, 903, 962, 1165, 1328
Hpy8I	GTNNAC	1 cut(s)	615
Hpy99I	CGWCG	2 cut(s)	97, 311
HpyAV	CCTTC	5 cut(s)	574, 758, 808, 961, 1033
HpyCH4III	ACNGT	2 cut(s)	663, 915
HpyCH4IV	ACGT	1 cut(s)	898
HpyF10VI	GCNNNNNNNGC	7 cut(s)	157, 221, 640, 677, 1112, 1361, 1391
HpyF3I	CTNAG	6 cut(s)	199, 255, 469, 509, 542, 672
HpySE526I	ACGT	1 cut(s)	898
Hsp92I	GRCGYC	1 cut(s)	149
Hsp92II	CATG	3 cut(s)	265, 416, 997
HspAI	GCGC	2 cut(s)	149, 751
KasI	GGCGCC	1 cut(s)	148
KspAI	GTTAAC	1 cut(s)	615
Kzo9I	GATC	7 cut(s)	163, 258, 502, 862, 1255, 1420, 1452
LmnI	GCTCC	2 cut(s)	274, 628
Lsp1109I	GCAGC	7 cut(s)	400, 630, 643, 1342, 1424, 1427, 1485
LweI	GCATC	6 cut(s)	336, 370, 441, 721, 841, 1065
MaeI	CTAG	4 cut(s)	57, 153, 225, 1241
MaeII	ACGT	1 cut(s)	898
MaeIII	GTNAC	3 cut(s)	59, 101, 915
MalI	GATC	7 cut(s)	165, 260, 504, 864, 1257, 1422, 1454
MboI	GATC	7 cut(s)	163, 258, 502, 862, 1255, 1420, 1452
MboII	GAAGA	8 cut(s)	340, 707, 710, 832, 956, 961, 1024, 1169
MfeI	CAATTG	2 cut(s)	638, 1485
MflI	RGATCY	2 cut(s)	502, 1255
MhlI	GDGCHC	1 cut(s)	279
MlsI	TGGCCA	1 cut(s)	84
MluNI	TGGCCA	1 cut(s)	84
Mly113I	GGCGCC	1 cut(s)	149
MmeI	TCCRAC	2 cut(s)	116, 171
Mox20I	TGGCCA	1 cut(s)	84
MscI	TGGCCA	1 cut(s)	84
MseI	TTAA	8 cut(s)	614, 771, 786, 804, 1005, 1136, 1170, 1458
MslI	CAYNNNNRTG	2 cut(s)	1443, 1473
Msp20I	TGGCCA	1 cut(s)	84
MspCI	CTTAAG	1 cut(s)	803
MspI	CCGG	2 cut(s)	99, 113
MspR9I	CCNGG	2 cut(s)	22, 356
MunI	CAATTG	2 cut(s)	638, 1485
Mva1269I	GAATGC	1 cut(s)	673
MvaI	CCWGG	2 cut(s)	22, 356
MwoI	GCNNNNNNNGC	7 cut(s)	157, 221, 640, 677, 1112, 1361, 1391
NarI	GGCGCC	1 cut(s)	149
NdeII	GATC	7 cut(s)	163, 258, 502, 862, 1255, 1420, 1452
NheI	GCTAGC	1 cut(s)	224
NlaIII	CATG	3 cut(s)	265, 416, 997
NlaIV	GGNNCC	2 cut(s)	111, 150
NmuCI	GTSAC	3 cut(s)	59, 101, 915
PagI	TCATGA	1 cut(s)	412
PceI	AGGCCT	1 cut(s)	768
PcsI	WCGNNNNNNNCGW	1 cut(s)	471
PctI	GAATGC	1 cut(s)	673
PfeI	GAWTC	8 cut(s)	67, 88, 400, 450, 586, 878, 1310, 1373
PinAI	ACCGGT	1 cut(s)	98
PluTI	GGCGCC	1 cut(s)	152
Psp6I	CCWGG	2 cut(s)	20, 354
PspFI	CCCAGC	1 cut(s)	215
PspGI	CCWGG	2 cut(s)	20, 354
PspN4I	GGNNCC	2 cut(s)	111, 150
PspPI	GGNCC	2 cut(s)	649, 1069
PstI	CTGCAG	4 cut(s)	648, 933, 1360, 1414
PsuI	RGATCY	2 cut(s)	502, 1255
RsaI	GTAC	1 cut(s)	912
RsaNI	GTAC	1 cut(s)	911
RseI	CAYNNNNRTG	2 cut(s)	1443, 1473
SaqAI	TTAA	8 cut(s)	614, 771, 786, 804, 1005, 1136, 1170, 1458
Sau3AI	GATC	7 cut(s)	163, 258, 502, 862, 1255, 1420, 1452
Sau96I	GGNCC	2 cut(s)	649, 1069
ScrFI	CCNGG	2 cut(s)	22, 356
SduI	GDGCHC	1 cut(s)	279
SfaNI	GCATC	6 cut(s)	336, 370, 441, 721, 841, 1065
SfcI	CTRYAG	5 cut(s)	644, 929, 939, 1356, 1410
SfoI	GGCGCC	1 cut(s)	150
SmiMI	CAYNNNNRTG	2 cut(s)	1443, 1473
SmlI	CTYRAG	1 cut(s)	803
SmoI	CTYRAG	1 cut(s)	803
SseBI	AGGCCT	1 cut(s)	768
SsiI	CCGC	5 cut(s)	50, 53, 157, 325, 680
SspDI	GGCGCC	1 cut(s)	148
SspMI	CTAG	4 cut(s)	57, 153, 225, 1241
StuI	AGGCCT	1 cut(s)	768
StyD4I	CCNGG	2 cut(s)	20, 354
StyI	CCWWGG	1 cut(s)	152
TaaI	ACNGT	2 cut(s)	663, 915
TaiI	ACGT	1 cut(s)	901
TaqI	TCGA	4 cut(s)	178, 342, 378, 474
TatI	WGTACW	1 cut(s)	910
TauI	GCSGC	3 cut(s)	53, 56, 160
TfiI	GAWTC	8 cut(s)	67, 88, 400, 450, 586, 878, 1310, 1373
Tru1I	TTAA	8 cut(s)	614, 771, 786, 804, 1005, 1136, 1170, 1458
Tru9I	TTAA	8 cut(s)	614, 771, 786, 804, 1005, 1136, 1170, 1458
TscAI	CASTG	1 cut(s)	550
TseFI	GTSAC	3 cut(s)	59, 101, 915
TseI	GCWGC	7 cut(s)	388, 631, 643, 1355, 1412, 1415, 1498
Tsp45I	GTSAC	3 cut(s)	59, 101, 915
TspDTI	ATGAA	5 cut(s)	401, 620, 705, 996, 1173
TspGWI	ACGGA	1 cut(s)	632
TspRI	CASTG	1 cut(s)	550
Vha464I	CTTAAG	1 cut(s)	803
XapI	RAATTY	1 cut(s)	1109
XmaJI	CCTAGG	1 cut(s)	152
XspI	CTAG	4 cut(s)	57, 153, 225, 1241

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.