Rmu_sc0004527.1_g000008

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004527.1
OG0004128
Physical Location & Seq
Forward (+)
51754 .. 57041
5288 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1590 bp
atgcccatttgcaagactgttactccaactccgcgcttattttttgggaaaactggagagatcaggagttcccaatgtagctttatggtgggaaataaagttagctttttgaggcagagagctcagggcgctcagattagttatagatcgaggaagcacgctggagctctccatgctacatgtcttgctgataaaatcctggtggcaaacagaggagaaattgccgttcgtgttattcgcacggcgcatgagatgggcataccctgtgtggctgtttactcaacaatagacaaggatgcacttcatgtcaaattggctgatgaatcagtttgtattggtgaagcagcaagcagtcaatcgtacctagtggttccaaatgttttatctgctgccatcagtcgtaaatgtacaatgcttcatcctggatatggtttcctgtctgagaatgcatcttttgttgaaatgtgcaaagaacatggaatcaactttattggtcctaagcccgaaagtatccgtattatgggtgataaagcgactgccagagaaacaatgaagcaagcaggtgttccaactgtaccaggaagtgatggattactacagagcacagaggaaggaatcaagcttgccgatgagattggttatcctgttatgatcaaggcaacagcaggtggtggaggacgtggtatgcgtcttgctaaagagcctgaagagtttgtaaagttgctacaggcagcaaagagcgaggctgcagctgcttttggaaatgatggagtttatttggaaaagtatgtcgtgaatcctagacacattgaatttcaggttcttgcagataagtatggtaatgttgttcactttggagagcgtgattgtagcatccagagaaggaaccaaaagctgttagaagaagcaccctctcctgcattgaccccagaattgcgaaaagccatgggtgatgcagcagttgctgccgcagcatctattggttatattggtgttggaaccattgagttccttttggacgaaagaggttccttttacttcatggaaatgaacactaggattcaggttgaacatcctgtgacagaaatgatttcctctgttgacttgattgaagaacaaattcgtgttgctatgggagaaaaactccgatacacacaggatgatatcgtgctcagagggcattcaattgaatgtcgtatcaatgcagaagatgcttttaaaggattccgacctgggccaggtagaataacagcttacttacccgccggaggtccatttgttagaatggatagtcatgtttatcctgattatgtggttcctccaaactatgattcccttcttggaaagcttattgtttgggcaccaacaagagaaaaggcaattgatcggatgaaaagagctcttaacgacactattataacaggggttcctacaactattgaatatcataagctcatcctggagatagacgatttcaaaaatgggaaggttgatactgcttttattcccaagcatgaagaggagctagcagcgccccaacaagtgttgcctgcattcccacccaaagaactagctagtgcaagtacgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

529

Amino Acids

57.91

Weight (kDa)

6.69

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 64 - 171 1e-41 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 176 - 383 5.6e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 185 - 353 2.9e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 203 - 352 6.1e-07 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 397 - 503 4.5e-38 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1418
AarI CACCTGC 2 cut(s) 551, 656
Acc36I ACCTGC 2 cut(s) 551, 656
AccB1I GGYRCC 1 cut(s) 1360
AccII CGCG 1 cut(s) 34
AciI CCGC 3 cut(s) 32, 969, 1263
AcsI RAATTY 2 cut(s) 812, 1119
AcuI CTGAAG 1 cut(s) 726
AfaI GTAC 4 cut(s) 362, 409, 576, 1585
AfiI CCNNNNNNNGG 4 cut(s) 428, 520, 1238, 1268
AflIII ACRYGT 1 cut(s) 179
AgsI TTSAA 8 cut(s) 461, 812, 1070, 1112, 1185, 1190, 1442, 1477
AjiI CACGTC 1 cut(s) 680
AjnI CCWGG 6 cut(s) 198, 421, 577, 1231, 1237, 1458
AjuI GAANNNNNNNTTGG 2 cut(s) 1315, 1347
Alw21I GWGCWC 5 cut(s) 124, 169, 605, 1173, 1402
AlwNI CAGNNNCTG 1 cut(s) 965
AoxI GGCC 1 cut(s) 1235
ApoI RAATTY 2 cut(s) 812, 1119
Asp700I GAANNNNTTC 1 cut(s) 1119
AspLEI GCGC 4 cut(s) 36, 131, 247, 1534
AspS9I GGNCC 3 cut(s) 494, 1235, 1271
AsuHPI GGTGA 3 cut(s) 350, 536, 962
AsuNHI GCTAGC 1 cut(s) 1525
AvaII GGWCC 2 cut(s) 494, 1271
BaeGI GKGCMC 1 cut(s) 1363
BanI GGYRCC 1 cut(s) 1360
BanII GRGCYC 3 cut(s) 124, 169, 1402
Bbv12I GWGCWC 5 cut(s) 124, 169, 605, 1173, 1402
BccI CCATC 4 cut(s) 247, 401, 581, 761
BceAI ACGGC 2 cut(s) 209, 258
BciT130I CCWGG 6 cut(s) 200, 423, 579, 1233, 1239, 1460
BciVI GTATCC 1 cut(s) 521
BclI TGATCA 1 cut(s) 651
BfaI CTAG 6 cut(s) 365, 801, 1056, 1526, 1571, 1575
BfmI CTRYAG 3 cut(s) 596, 725, 747
BfoI RGCGCY 2 cut(s) 132, 1535
BfuAI ACCTGC 2 cut(s) 551, 656
BfuI GTATCC 1 cut(s) 521
Bme1390I CCNGG 6 cut(s) 200, 423, 579, 1233, 1239, 1460
Bme18I GGWCC 2 cut(s) 494, 1271
BmgBI CACGTC 1 cut(s) 680
BmgT120I GGNCC 3 cut(s) 494, 1235, 1271
BmiI GGNNCC 7 cut(s) 372, 887, 1000, 1030, 1317, 1362, 1428
BmrFI CCNGG 6 cut(s) 200, 423, 579, 1233, 1239, 1460
BmsI GCATC 6 cut(s) 286, 458, 882, 943, 983, 1201
BmtI GCTAGC 1 cut(s) 1529
BplI GAGNNNNNCTC 2 cut(s) 1128, 1160
BpmI CTGGAG 3 cut(s) 75, 183, 1481
Bpu10I CCTNAGC 2 cut(s) 123, 498
BsaAI YACGTR 1 cut(s) 1587
BsaJI CCNNGG 2 cut(s) 945, 1232
BsaXI ACNNNNNCTCC 4 cut(s) 7, 13, 37, 43
Bsc4I CCNNNNNNNGG 4 cut(s) 428, 520, 1238, 1268
Bse1I ACTGG 1 cut(s) 58
BseBI CCWGG 6 cut(s) 200, 423, 579, 1233, 1239, 1460
BseDI CCNNGG 2 cut(s) 945, 1232
BseGI GGATG 7 cut(s) 301, 418, 873, 1072, 1165, 1395, 1455
BseLI CCNNNNNNNGG 4 cut(s) 428, 520, 1238, 1268
BseMII CTCAG 4 cut(s) 137, 146, 432, 1186
BseNI ACTGG 1 cut(s) 58
BseRI GAGGAG 2 cut(s) 228, 1535
BseSI GKGCMC 1 cut(s) 1363
Bsh1236I CGCG 1 cut(s) 34
BshFI GGCC 1 cut(s) 1237
BshNI GGYRCC 1 cut(s) 1360
BsiHKAI GWGCWC 5 cut(s) 124, 169, 605, 1173, 1402
BsiSI CCGG 1 cut(s) 1266
BslI CCNNNNNNNGG 4 cut(s) 428, 520, 1238, 1268
BsmI GAATGC 3 cut(s) 451, 1180, 1553
BsnI GGCC 1 cut(s) 1237
Bsp1286I GDGCHC 6 cut(s) 124, 169, 605, 1173, 1363, 1402
Bsp1407I TGTACA 1 cut(s) 407
Bsp143I GATC 4 cut(s) 60, 146, 651, 1384
Bsp19I CCATGG 1 cut(s) 945
BspACI CCGC 3 cut(s) 32, 969, 1263
BspANI GGCC 1 cut(s) 1237
BspCNI CTCAG 4 cut(s) 136, 145, 433, 1185
BspFNI CGCG 1 cut(s) 34
BspLI GGNNCC 7 cut(s) 372, 887, 1000, 1030, 1317, 1362, 1428
BspMAI CTGCAG 1 cut(s) 751
BspMI ACCTGC 2 cut(s) 551, 656
BspOI GCTAGC 1 cut(s) 1529
BspT107I GGYRCC 1 cut(s) 1360
BsrGI TGTACA 1 cut(s) 407
BsrI ACTGG 1 cut(s) 58
BssECI CCNNGG 2 cut(s) 945, 1232
BssMI GATC 4 cut(s) 60, 146, 651, 1384
BssT1I CCWWGG 1 cut(s) 945
Bst2UI CCWGG 6 cut(s) 200, 423, 579, 1233, 1239, 1460
Bst4CI ACNGT 2 cut(s) 19, 574
Bst6I CTCTTC 2 cut(s) 702, 1512
BstAPI GCANNNNNTGC 3 cut(s) 962, 965, 1211
BstAUI TGTACA 1 cut(s) 407
BstBAI YACGTR 1 cut(s) 1587
BstC8I GCNNGC 6 cut(s) 159, 349, 558, 624, 1527, 1551
BstDEI CTNAG 5 cut(s) 123, 132, 441, 498, 1172
BstDSI CCRYGG 1 cut(s) 945
BstENI CCTNNNNNAGG 1 cut(s) 1236
BstF5I GGATG 7 cut(s) 301, 418, 873, 1072, 1165, 1395, 1455
BstFNI CGCG 1 cut(s) 34
BstH2I RGCGCY 2 cut(s) 132, 1535
BstHHI GCGC 4 cut(s) 36, 131, 247, 1534
BstKTI GATC 4 cut(s) 63, 149, 654, 1387
BstMBI GATC 4 cut(s) 60, 146, 651, 1384
BstMWI GCNNNNNNNGC 9 cut(s) 128, 173, 752, 962, 965, 971, 1177, 1211, 1531
BstNI CCWGG 6 cut(s) 200, 423, 579, 1233, 1239, 1460
BstNSI RCATGY 1 cut(s) 183
BstSCI CCNGG 6 cut(s) 198, 421, 577, 1231, 1237, 1458
BstSFI CTRYAG 3 cut(s) 596, 725, 747
BstSLI GKGCMC 1 cut(s) 1363
BstSNI TACGTA 1 cut(s) 1587
BstUI CGCG 1 cut(s) 34
BsuI GTATCC 1 cut(s) 521
BsuRI GGCC 1 cut(s) 1237
BtgI CCRYGG 1 cut(s) 945
BtrI CACGTC 1 cut(s) 680
BtsCI GGATG 7 cut(s) 301, 418, 873, 1072, 1165, 1395, 1455
BveI ACCTGC 2 cut(s) 551, 656
Cac8I GCNNGC 6 cut(s) 159, 349, 558, 624, 1527, 1551
CaiI CAGNNNCTG 1 cut(s) 965
CfoI GCGC 4 cut(s) 36, 131, 247, 1534
Cfr13I GGNCC 3 cut(s) 494, 1235, 1271
CseI GACGC 1 cut(s) 677
Csp6I GTAC 4 cut(s) 361, 408, 575, 1584
CviAII CATG 9 cut(s) 173, 180, 248, 305, 476, 946, 1042, 1295, 1514
CviQI GTAC 4 cut(s) 361, 408, 575, 1584
DdeI CTNAG 5 cut(s) 123, 132, 441, 498, 1172
DpnI GATC 4 cut(s) 62, 148, 653, 1386
DpnII GATC 4 cut(s) 60, 146, 651, 1384
DraI TTTAAA 1 cut(s) 1219
Eam1104I CTCTTC 2 cut(s) 702, 1512
EarI CTCTTC 2 cut(s) 702, 1512
Ecl136II GAGCTC 3 cut(s) 122, 167, 1400
Eco105I TACGTA 1 cut(s) 1587
Eco130I CCWWGG 1 cut(s) 945
Eco24I GRGCYC 3 cut(s) 124, 169, 1402
Eco32I GATATC 1 cut(s) 1165
Eco47I GGWCC 2 cut(s) 494, 1271
Eco53kI GAGCTC 3 cut(s) 122, 167, 1400
Eco57I CTGAAG 1 cut(s) 726
EcoICRI GAGCTC 3 cut(s) 122, 167, 1400
EcoNI CCTNNNNNAGG 1 cut(s) 1236
EcoRII CCWGG 6 cut(s) 198, 421, 577, 1231, 1237, 1458
EcoRV GATATC 1 cut(s) 1165
EcoT14I CCWWGG 1 cut(s) 945
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 3 cut(s) 124, 169, 1402
ErhI CCWWGG 1 cut(s) 945
FaeI CATG 9 cut(s) 176, 183, 251, 308, 479, 949, 1045, 1298, 1517
FatI CATG 9 cut(s) 172, 179, 247, 304, 475, 945, 1041, 1294, 1513
FauI CCCGC 1 cut(s) 1270
FbaI TGATCA 1 cut(s) 651
FokI GGATG 7 cut(s) 308, 405, 860, 1059, 1172, 1402, 1442
FriOI GRGCYC 3 cut(s) 124, 169, 1402
FspBI CTAG 6 cut(s) 365, 801, 1056, 1526, 1571, 1575
GlaI GCGC 4 cut(s) 35, 130, 246, 1533
GsuI CTGGAG 3 cut(s) 75, 183, 1481
HaeII RGCGCY 2 cut(s) 132, 1535
HaeIII GGCC 1 cut(s) 1237
HapII CCGG 1 cut(s) 1266
HgaI GACGC 1 cut(s) 677
HhaI GCGC 4 cut(s) 36, 131, 247, 1534
Hin1II CATG 9 cut(s) 176, 183, 251, 308, 479, 949, 1045, 1298, 1517
Hin6I GCGC 4 cut(s) 34, 129, 245, 1532
HinP1I GCGC 4 cut(s) 34, 129, 245, 1532
HincII GTYRAC 1 cut(s) 1102
HindII GTYRAC 1 cut(s) 1102
HindIII AAGCTT 2 cut(s) 620, 1346
HinfI GANTC 7 cut(s) 323, 480, 615, 796, 1060, 1224, 1331
HpaII CCGG 1 cut(s) 1266
HphI GGTGA 3 cut(s) 350, 536, 962
Hpy166II GTNNAC 3 cut(s) 277, 850, 1102
Hpy188I TCNGA 6 cut(s) 135, 442, 1148, 1175, 1229, 1389
Hpy188III TCNNGA 4 cut(s) 64, 793, 877, 1304
Hpy8I GTNNAC 3 cut(s) 277, 850, 1102
HpyAV CCTTC 4 cut(s) 605, 876, 1346, 1480
HpyCH4III ACNGT 2 cut(s) 19, 574
HpyCH4IV ACGT 2 cut(s) 679, 1586
HpyF10VI GCNNNNNNNGC 9 cut(s) 128, 173, 752, 962, 965, 971, 1177, 1211, 1531
HpyF3I CTNAG 5 cut(s) 123, 132, 441, 498, 1172
HpySE526I ACGT 2 cut(s) 679, 1586
Hsp92II CATG 9 cut(s) 176, 183, 251, 308, 479, 949, 1045, 1298, 1517
HspAI GCGC 4 cut(s) 34, 129, 245, 1532
Ksp22I TGATCA 1 cut(s) 651
Kzo9I GATC 4 cut(s) 60, 146, 651, 1384
LmnI GCTCC 2 cut(s) 164, 1522
LweI GCATC 6 cut(s) 286, 458, 882, 943, 983, 1201
MaeI CTAG 6 cut(s) 365, 801, 1056, 1526, 1571, 1575
MaeII ACGT 2 cut(s) 679, 1586
MaeIII GTNAC 2 cut(s) 19, 1078
MalI GATC 4 cut(s) 62, 148, 653, 1386
MboI GATC 4 cut(s) 60, 146, 651, 1384
MboII GAAGA 5 cut(s) 719, 914, 1124, 1220, 1529
MfeI CAATTG 2 cut(s) 1185, 1380
MhlI GDGCHC 6 cut(s) 124, 169, 605, 1173, 1363, 1402
MluCI AATT 7 cut(s) 219, 311, 812, 932, 1119, 1185, 1380
MmeI TCCRAC 4 cut(s) 50, 593, 976, 1252
Mph1103I ATGCAT 1 cut(s) 451
MroXI GAANNNNTTC 1 cut(s) 1119
MseI TTAA 2 cut(s) 1218, 1404
MslI CAYNNNNRTG 1 cut(s) 1046
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 1 cut(s) 1266
MspR9I CCNGG 6 cut(s) 200, 423, 579, 1233, 1239, 1460
MunI CAATTG 2 cut(s) 1185, 1380
Mva1269I GAATGC 3 cut(s) 451, 1180, 1553
MvaI CCWGG 6 cut(s) 200, 423, 579, 1233, 1239, 1460
MvnI CGCG 1 cut(s) 34
MwoI GCNNNNNNNGC 9 cut(s) 128, 173, 752, 962, 965, 971, 1177, 1211, 1531
NcoI CCATGG 1 cut(s) 945
NdeII GATC 4 cut(s) 60, 146, 651, 1384
NheI GCTAGC 1 cut(s) 1525
NlaIII CATG 9 cut(s) 176, 183, 251, 308, 479, 949, 1045, 1298, 1517
NlaIV GGNNCC 7 cut(s) 372, 887, 1000, 1030, 1317, 1362, 1428
NmuCI GTSAC 1 cut(s) 1078
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 1 cut(s) 183
PaqCI CACCTGC 2 cut(s) 551, 656
PciI ACATGT 1 cut(s) 179
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PctI GAATGC 3 cut(s) 451, 1180, 1553
PdmI GAANNNNTTC 1 cut(s) 1119
PfeI GAWTC 7 cut(s) 323, 480, 615, 796, 1060, 1224, 1331
PfoI TCCNGGA 2 cut(s) 421, 1458
Ppu21I YACGTR 1 cut(s) 1587
PscI ACATGT 1 cut(s) 179
PsiI TTATAA 1 cut(s) 1418
Psp124BI GAGCTC 3 cut(s) 124, 169, 1402
Psp6I CCWGG 6 cut(s) 198, 421, 577, 1231, 1237, 1458
PspGI CCWGG 6 cut(s) 198, 421, 577, 1231, 1237, 1458
PspN4I GGNNCC 7 cut(s) 372, 887, 1000, 1030, 1317, 1362, 1428
PspPI GGNCC 3 cut(s) 494, 1235, 1271
PstI CTGCAG 1 cut(s) 751
PstNI CAGNNNCTG 1 cut(s) 965
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 4 cut(s) 362, 409, 576, 1585
RsaNI GTAC 4 cut(s) 361, 408, 575, 1584
RseI CAYNNNNRTG 1 cut(s) 1046
SacI GAGCTC 3 cut(s) 124, 169, 1402
SaqAI TTAA 2 cut(s) 1218, 1404
Sau3AI GATC 4 cut(s) 60, 146, 651, 1384
Sau96I GGNCC 3 cut(s) 494, 1235, 1271
ScrFI CCNGG 6 cut(s) 200, 423, 579, 1233, 1239, 1460
SduI GDGCHC 6 cut(s) 124, 169, 605, 1173, 1363, 1402
SfaNI GCATC 6 cut(s) 286, 458, 882, 943, 983, 1201
SfcI CTRYAG 3 cut(s) 596, 725, 747
SinI GGWCC 2 cut(s) 494, 1271
SmiMI CAYNNNNRTG 1 cut(s) 1046
SnaBI TACGTA 1 cut(s) 1587
Sse9I AATT 7 cut(s) 219, 311, 812, 932, 1119, 1185, 1380
SsiI CCGC 3 cut(s) 32, 969, 1263
SspMI CTAG 6 cut(s) 365, 801, 1056, 1526, 1571, 1575
SstI GAGCTC 3 cut(s) 124, 169, 1402
StyD4I CCNGG 6 cut(s) 198, 421, 577, 1231, 1237, 1458
StyI CCWWGG 1 cut(s) 945
TaaI ACNGT 2 cut(s) 19, 574
TaiI ACGT 2 cut(s) 682, 1589
TaqI TCGA 1 cut(s) 149
TasI AATT 7 cut(s) 219, 311, 812, 932, 1119, 1185, 1380
TatI WGTACW 1 cut(s) 407
TauI GCSGC 1 cut(s) 971
TfiI GAWTC 7 cut(s) 323, 480, 615, 796, 1060, 1224, 1331
Tru1I TTAA 2 cut(s) 1218, 1404
Tru9I TTAA 2 cut(s) 1218, 1404
TseFI GTSAC 1 cut(s) 1078
Tsp45I GTSAC 1 cut(s) 1078
TspDTI ATGAA 8 cut(s) 293, 336, 407, 566, 1030, 1064, 1406, 1530
TspGWI ACGGA 1 cut(s) 503
VpaK11BI GGWCC 2 cut(s) 494, 1271
XagI CCTNNNNNAGG 1 cut(s) 1236
XapI RAATTY 2 cut(s) 812, 1119
XceI RCATGY 1 cut(s) 183
XmnI GAANNNNTTC 1 cut(s) 1119
XspI CTAG 6 cut(s) 365, 801, 1056, 1526, 1571, 1575
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.