Rmu_sc0004529.1_g000001

Epimerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004529.1
OG0003144
Physical Location & Seq
Forward (+)
1 .. 4802
4802 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 755 bp
ataatcatagtgtgcacgtcttgacacggtctagatcaaaggctgagttaatttttccggtcaaggactttccaggaattgtaattgcagaggagccagagtggaaagatagcattcgaggttcaactggtgttgtaaatttggctggaatgcccattagtacaagatggacttctgagataaagaaagagatcaagcagagcagggttagagtcacctctaaggtcatagatttaataaatgatttaccagatgcggtccgaccttcagtcttggttagtgcaacagccgtcggttactatggtactagtgagacacgcgtatttgatgaacagagtccatcaggaaatgattacttggctgaggtttgcagagaatgggaaggaactgctctcaaagtaaataaggatgttcggttagcgctcattcgtattggtgttgttcttggtaaagatggaggagctttagctaaaatgatccctctcttcatggtttttgctgggggacccttgggctctggaaaacaatggttttcctggattcatctggatgatatagtgaacctgatatatgaagctctctctaatccatcttataaaggagttatcaatggaactgcacccaaccctgttcgattcacagaaatgtgtcaacatttgggaaatgtcttgggtaggccttcatggctgcctgtgcctgatttcgccttgaaagcagtccttggagaaggcgcttcagtggtgagcatttgttga

Protein Analysis

250

Amino Acids

27.23

Weight (kDa)

7.88

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 2 - 197 2.6e-10 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 596
AccII CGCG 1 cut(s) 320
AciI CCGC 1 cut(s) 256
AclWI GGATC 1 cut(s) 471
AcsI RAATTY 1 cut(s) 138
AcuI CTGAAG 2 cut(s) 251, 719
AfaI GTAC 2 cut(s) 162, 306
AfeI AGCGCT 1 cut(s) 422
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 2 cut(s) 125, 711
AhdI GACNNNNNGTC 1 cut(s) 268
AhlI ACTAGT 1 cut(s) 307
AjiI CACGTC 1 cut(s) 18
AjnI CCWGG 2 cut(s) 72, 535
AluBI AGCT 3 cut(s) 463, 469, 577
AluI AGCT 3 cut(s) 463, 469, 577
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 307
Alw44I GTGCAC 1 cut(s) 13
AlwI GGATC 1 cut(s) 471
Aor51HI AGCGCT 1 cut(s) 422
AoxI GGCC 1 cut(s) 676
ApaLI GTGCAC 1 cut(s) 13
ApeKI GCWGC 1 cut(s) 687
ApoI RAATTY 1 cut(s) 138
AspLEI GCGC 2 cut(s) 423, 733
AspS9I GGNCC 2 cut(s) 258, 505
AsuHPI GGTGA 2 cut(s) 207, 753
AvaII GGWCC 2 cut(s) 258, 505
BaeGI GKGCMC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 517
Bbv12I GWGCWC 1 cut(s) 17
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 1 cut(s) 674
BccI CCATC 4 cut(s) 161, 348, 448, 597
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 2 cut(s) 74, 537
BcoDI GTCTC 1 cut(s) 307
BcuI ACTAGT 1 cut(s) 307
BfaI CTAG 2 cut(s) 32, 308
BfoI RGCGCY 2 cut(s) 424, 734
BglI GCCNNNNNGGC 1 cut(s) 684
BisI GCNGC 1 cut(s) 688
BlsI GCNGC 1 cut(s) 689
Bme1390I CCNGG 2 cut(s) 74, 537
Bme18I GGWCC 2 cut(s) 258, 505
BmeRI GACNNNNNGTC 1 cut(s) 268
BmgBI CACGTC 1 cut(s) 18
BmgT120I GGNCC 2 cut(s) 258, 505
BmiI GGNNCC 3 cut(s) 95, 506, 507
BmrFI CCNGG 2 cut(s) 74, 537
BmsI GCATC 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 362
BsaJI CCNNGG 2 cut(s) 509, 720
BsaWI WCCGGW 1 cut(s) 57
BsaXI ACNNNNNCTCC 2 cut(s) 85, 115
Bse1I ACTGG 1 cut(s) 132
BseBI CCWGG 2 cut(s) 74, 537
BseDI CCNNGG 2 cut(s) 509, 720
BseGI GGATG 2 cut(s) 414, 555
BseMII CTCAG 3 cut(s) 35, 167, 353
BseNI ACTGG 1 cut(s) 132
BseRI GAGGAG 2 cut(s) 106, 473
BseSI GKGCMC 1 cut(s) 17
BseXI GCAGC 1 cut(s) 674
BseYI CCCAGC 1 cut(s) 499
BsgI GTGCAG 1 cut(s) 602
Bsh1236I CGCG 1 cut(s) 320
BshFI GGCC 1 cut(s) 678
BsiHKAI GWGCWC 1 cut(s) 17
BsiSI CCGG 1 cut(s) 58
BslFI GGGAC 1 cut(s) 518
BsmAI GTCTC 1 cut(s) 307
BsmFI GGGAC 1 cut(s) 518
BsmI GAATGC 2 cut(s) 113, 155
BsnI GGCC 1 cut(s) 678
Bsp1286I GDGCHC 2 cut(s) 17, 517
Bsp143I GATC 3 cut(s) 34, 191, 476
BspACI CCGC 1 cut(s) 256
BspANI GGCC 1 cut(s) 678
BspCNI CTCAG 3 cut(s) 36, 168, 354
BspFNI CGCG 1 cut(s) 320
BspLI GGNNCC 3 cut(s) 95, 506, 507
BspPI GGATC 1 cut(s) 471
BsrI ACTGG 1 cut(s) 132
BssECI CCNNGG 2 cut(s) 509, 720
BssMI GATC 3 cut(s) 34, 191, 476
BssT1I CCWWGG 2 cut(s) 509, 720
Bst2UI CCWGG 2 cut(s) 74, 537
Bst4CI ACNGT 1 cut(s) 29
Bst6I CTCTTC 1 cut(s) 490
BstDEI CTNAG 4 cut(s) 44, 176, 221, 362
BstF5I GGATG 2 cut(s) 414, 555
BstFNI CGCG 1 cut(s) 320
BstH2I RGCGCY 2 cut(s) 424, 734
BstHHI GCGC 2 cut(s) 423, 733
BstKTI GATC 3 cut(s) 37, 194, 479
BstMAI GTCTC 1 cut(s) 307
BstMBI GATC 3 cut(s) 34, 191, 476
BstMWI GCNNNNNNNGC 3 cut(s) 684, 693, 712
BstNI CCWGG 2 cut(s) 74, 537
BstSCI CCNGG 2 cut(s) 72, 535
BstSLI GKGCMC 1 cut(s) 17
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 1 cut(s) 674
BsuRI GGCC 1 cut(s) 678
BtrI CACGTC 1 cut(s) 18
BtsCI GGATG 2 cut(s) 414, 555
BtsIMutI CAGTG 1 cut(s) 743
CfoI GCGC 2 cut(s) 423, 733
Cfr13I GGNCC 2 cut(s) 258, 505
CpoI CGGWCCG 1 cut(s) 258
Csp6I GTAC 2 cut(s) 161, 305
CspI CGGWCCG 1 cut(s) 258
CviAII CATG 2 cut(s) 489, 683
CviQI GTAC 2 cut(s) 161, 305
DdeI CTNAG 4 cut(s) 44, 176, 221, 362
DpnI GATC 3 cut(s) 36, 193, 478
DpnII GATC 3 cut(s) 34, 191, 476
DriI GACNNNNNGTC 1 cut(s) 268
Eam1104I CTCTTC 1 cut(s) 490
Eam1105I GACNNNNNGTC 1 cut(s) 268
EarI CTCTTC 1 cut(s) 490
Eco130I CCWWGG 2 cut(s) 509, 720
Eco147I AGGCCT 1 cut(s) 678
Eco24I GRGCYC 1 cut(s) 517
Eco47I GGWCC 2 cut(s) 258, 505
Eco47III AGCGCT 1 cut(s) 422
Eco57I CTGAAG 2 cut(s) 251, 719
EcoO109I RGGNCCY 1 cut(s) 505
EcoRII CCWGG 2 cut(s) 72, 535
EcoT14I CCWWGG 2 cut(s) 509, 720
EcoT38I GRGCYC 1 cut(s) 517
ErhI CCWWGG 2 cut(s) 509, 720
FaeI CATG 2 cut(s) 492, 686
FaiI YATR 9 cut(s) 8, 229, 302, 490, 556, 570, 572, 596, 684
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 704, 736
FaqI GGGAC 1 cut(s) 518
FatI CATG 2 cut(s) 488, 682
Fnu4HI GCNGC 1 cut(s) 688
FokI GGATG 2 cut(s) 421, 562
FriOI GRGCYC 1 cut(s) 517
Fsp4HI GCNGC 1 cut(s) 688
FspBI CTAG 2 cut(s) 32, 308
GlaI GCGC 2 cut(s) 422, 732
GluI GCNGC 1 cut(s) 688
GsaI CCCAGC 1 cut(s) 503
HaeII RGCGCY 2 cut(s) 424, 734
HaeIII GGCC 1 cut(s) 678
HapII CCGG 1 cut(s) 58
HhaI GCGC 2 cut(s) 423, 733
Hin1II CATG 2 cut(s) 492, 686
Hin6I GCGC 2 cut(s) 421, 731
HinP1I GCGC 2 cut(s) 421, 731
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HinfI GANTC 4 cut(s) 212, 336, 540, 635
HpaII CCGG 1 cut(s) 58
HphI GGTGA 2 cut(s) 207, 753
Hpy166II GTNNAC 3 cut(s) 15, 561, 652
Hpy188I TCNGA 2 cut(s) 177, 262
Hpy188III TCNNGA 5 cut(s) 21, 32, 344, 518, 547
Hpy8I GTNNAC 3 cut(s) 15, 561, 652
Hpy99I CGWCG 1 cut(s) 295
HpyAV CCTTC 4 cut(s) 275, 376, 689, 721
HpyCH4III ACNGT 1 cut(s) 29
HpyCH4IV ACGT 1 cut(s) 17
HpyCH4V TGCA 5 cut(s) 15, 88, 283, 371, 619
HpyF10VI GCNNNNNNNGC 3 cut(s) 684, 693, 712
HpyF3I CTNAG 4 cut(s) 44, 176, 221, 362
HpySE526I ACGT 1 cut(s) 17
Hsp92II CATG 2 cut(s) 492, 686
HspAI GCGC 2 cut(s) 421, 731
KflI GGGWCCC 1 cut(s) 505
Kzo9I GATC 3 cut(s) 34, 191, 476
LmnI GCTCC 2 cut(s) 93, 460
Lsp1109I GCAGC 1 cut(s) 674
LweI GCATC 1 cut(s) 243
MaeI CTAG 2 cut(s) 32, 308
MaeII ACGT 1 cut(s) 17
MaeIII GTNAC 2 cut(s) 213, 295
MalI GATC 3 cut(s) 36, 193, 478
MboI GATC 3 cut(s) 34, 191, 476
MboII GAAGA 1 cut(s) 477
MhlI GDGCHC 2 cut(s) 17, 517
MluCI AATT 4 cut(s) 50, 77, 83, 138
MluI ACGCGT 1 cut(s) 318
MlyI GAGTC 2 cut(s) 221, 345
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 6 cut(s) 84, 112, 228, 357, 451, 491
MseI TTAA 2 cut(s) 49, 235
MslI CAYNNNNRTG 2 cut(s) 548, 643
MspI CCGG 1 cut(s) 58
MspR9I CCNGG 2 cut(s) 74, 537
Mva1269I GAATGC 2 cut(s) 113, 155
MvaI CCWGG 2 cut(s) 74, 537
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 3 cut(s) 684, 693, 712
NdeII GATC 3 cut(s) 34, 191, 476
NlaIII CATG 2 cut(s) 492, 686
NlaIV GGNNCC 3 cut(s) 95, 506, 507
NmuCI GTSAC 1 cut(s) 213
PceI AGGCCT 1 cut(s) 678
PctI GAATGC 2 cut(s) 113, 155
PfeI GAWTC 2 cut(s) 540, 635
PflFI GACNNNGTC 1 cut(s) 27
PfoI TCCNGGA 2 cut(s) 72, 535
PkrI GCNGC 1 cut(s) 689
PleI GAGTC 2 cut(s) 220, 344
PpsI GAGTC 2 cut(s) 220, 344
PpuMI RGGWCCY 1 cut(s) 505
PsiI TTATAA 1 cut(s) 596
Psp5II RGGWCCY 1 cut(s) 505
Psp6I CCWGG 2 cut(s) 72, 535
PspFI CCCAGC 1 cut(s) 499
PspGI CCWGG 2 cut(s) 72, 535
PspN4I GGNNCC 3 cut(s) 95, 506, 507
PspPI GGNCC 2 cut(s) 258, 505
PspPPI RGGWCCY 1 cut(s) 505
PsyI GACNNNGTC 1 cut(s) 27
RsaI GTAC 2 cut(s) 162, 306
RsaNI GTAC 2 cut(s) 161, 305
RseI CAYNNNNRTG 2 cut(s) 548, 643
Rsr2I CGGWCCG 1 cut(s) 258
RsrII CGGWCCG 1 cut(s) 258
SaqAI TTAA 2 cut(s) 49, 235
SatI GCNGC 1 cut(s) 688
Sau3AI GATC 3 cut(s) 34, 191, 476
Sau96I GGNCC 2 cut(s) 258, 505
SchI GAGTC 2 cut(s) 221, 345
ScrFI CCNGG 2 cut(s) 74, 537
SduI GDGCHC 2 cut(s) 17, 517
SfaNI GCATC 1 cut(s) 243
SinI GGWCC 2 cut(s) 258, 505
SmiMI CAYNNNNRTG 2 cut(s) 548, 643
SpeI ACTAGT 1 cut(s) 307
Sse9I AATT 4 cut(s) 50, 77, 83, 138
SseBI AGGCCT 1 cut(s) 678
SsiI CCGC 1 cut(s) 256
SspMI CTAG 2 cut(s) 32, 308
StuI AGGCCT 1 cut(s) 678
StyD4I CCNGG 2 cut(s) 72, 535
StyI CCWWGG 2 cut(s) 509, 720
TaaI ACNGT 1 cut(s) 29
TaiI ACGT 1 cut(s) 20
TaqI TCGA 2 cut(s) 117, 633
TasI AATT 4 cut(s) 50, 77, 83, 138
TatI WGTACW 1 cut(s) 160
TfiI GAWTC 2 cut(s) 540, 635
Tru1I TTAA 2 cut(s) 49, 235
Tru9I TTAA 2 cut(s) 49, 235
TscAI CASTG 1 cut(s) 743
TseFI GTSAC 1 cut(s) 213
TseI GCWGC 1 cut(s) 687
Tsp45I GTSAC 1 cut(s) 213
TspDTI ATGAA 5 cut(s) 344, 477, 532, 587, 671
TspRI CASTG 1 cut(s) 743
Tth111I GACNNNGTC 1 cut(s) 27
VneI GTGCAC 1 cut(s) 13
VpaK11BI GGWCC 2 cut(s) 258, 505
XapI RAATTY 1 cut(s) 138
XbaI TCTAGA 1 cut(s) 31
XspI CTAG 2 cut(s) 32, 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.