Rmu_sc0004668.1_g000002

ribosomal protein s10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004668.1
OG0003882
Physical Location & Seq
Reverse (-)
9020 .. 14827
5808 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1479 bp
atgtcgatcgaggagcccggcaccacatcagtcccacaggggactacaaaggccaatccaacgacggcgaaagggctcgcagccggacaggaggaaatcccattgttttctcgtcaagctctcctcgtctggtggcgccctcattctagcgctggtctctggcagtgctggtgtgtagtggttgctgccagacgggctacatacggtttccggtggtgtggagattgggtggttgcacttggtgtctcttttgtctggttggcggcgatggtttctttgaattggcttcttaccttggttaaggccaggacagcggtcatggctagctttgctagttgggctgacagggttttggtcaatggtgtggtcaatggtggaagggctctcggtggctggatagttggttctgtggtttgccacgtggggttcatgagagcctggtgttggtctggcggtttggggtggagagatgttcatgggtgggggcgggtctccgtgcaagggtggaattacagaggcgagttcgggttaggggaggttccatgcggtgggtttgctattcagggcccattgcggtttagatttggtatgggggagttggtcactgacctcactattccagcaacagggatttccaaatggtcgaggtcattcactcaacgatgggacaaggttcttgacggcggtcttttgagttttagtagggtttctttttttgttagacacggtgctgaagaggacgcagtcttctgtgcagttgattacgaggtattactggaacttttgggatcatatgatcaatgtagccttggaaaggggcgtattgtgattagagtttgggccattggcctaatggtttctgtaactgcagtttcttgggctcgcaggtctaccagcaaaaaacccagctccgaacaatccaatgaagatgaggacaatgctgacaccaagtccaggcagtccgactccacaatcaaaggtacgaccagttctcctggcagcaatgagaccaaccttccgcccttcaccgccgactgctgtggtttcatcgcaaaacgggccgtcgaatcgccgatagttagggaaatctccattatagtcattgaggttggagtagcagtgatgacatccagaatatgtgttgcgattcgatcttttgagaaatatccattgggacttctgccttgcacgcggaggattcagttgcctgacacacgcgtcttatataccgtgttacgatcacctcatattgataagaagtccagagaacaatttcatatgataaccaagaaactacttctggtcatagacgcaaaagcaaatgagttgcaaaagaagttcttttggttaaaacaccagcgtatacttggagctcaatatgaaattatattcaactcaaagacccgtttggataacggaaaactgcagactttggttaataaggaaactgacacaactcctgcagcagaattggggtaa

Protein Analysis

492

Amino Acids

54.4

Weight (kDa)

9.3

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S10 PF00338 395 - 441 2.9e-08 Ribosomal protein S10p/S20e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1217
Acc36I ACCTGC 1 cut(s) 876
AccB1I GGYRCC 2 cut(s) 20, 135
AccB7I CCANNNNNTGG 1 cut(s) 548
AccI GTMKAC 2 cut(s) 890, 1363
AccII CGCG 2 cut(s) 1192, 1218
AclWI GGATC 1 cut(s) 796
AcuI CTGAAG 1 cut(s) 753
AcvI CACGTG 1 cut(s) 421
AcyI GRCGYC 1 cut(s) 136
AdeI CACNNNGTG 1 cut(s) 242
AfaI GTAC 1 cut(s) 982
AfeI AGCGCT 1 cut(s) 151
AfiI CCNNNNNNNGG 6 cut(s) 444, 501, 548, 626, 814, 954
AflIII ACRYGT 1 cut(s) 1216
AgsI TTSAA 2 cut(s) 280, 1393
AhdI GACNNNNNGTC 1 cut(s) 949
AjnI CCWGG 4 cut(s) 305, 437, 953, 994
AjuI GAANNNNNNNTTGG 2 cut(s) 1154, 1186
AloI GAACNNNNNNTCC 2 cut(s) 388, 420
AluBI AGCT 4 cut(s) 119, 327, 909, 1373
AluI AGCT 4 cut(s) 119, 327, 909, 1373
Alw21I GWGCWC 1 cut(s) 1375
Alw26I GTCTC 4 cut(s) 161, 250, 496, 1001
AlwI GGATC 1 cut(s) 796
Aor51HI AGCGCT 1 cut(s) 151
AoxI GGCC 6 cut(s) 51, 303, 565, 840, 847, 1059
ApaI GGGCCC 1 cut(s) 569
ApeKI GCWGC 4 cut(s) 80, 185, 999, 1463
Asp700I GAANNNNTTC 1 cut(s) 1272
AspLEI GCGC 2 cut(s) 138, 152
AspS9I GGNCC 4 cut(s) 565, 566, 840, 1059
AsuC2I CCSGG 1 cut(s) 18
AsuHPI GGTGA 2 cut(s) 1018, 1233
AsuNHI GCTAGC 1 cut(s) 323
BaeGI GKGCMC 1 cut(s) 569
BanI GGYRCC 2 cut(s) 20, 135
BanII GRGCYC 6 cut(s) 18, 78, 385, 569, 883, 1375
BbrPI CACGTG 1 cut(s) 421
BbsI GAAGAC 1 cut(s) 739
Bbv12I GWGCWC 1 cut(s) 1375
BbvI GCAGC 4 cut(s) 92, 172, 1011, 1475
BccI CCATC 2 cut(s) 262, 657
BceAI ACGGC 3 cut(s) 81, 697, 1046
BciT130I CCWGG 4 cut(s) 307, 439, 955, 996
BclI TGATCA 1 cut(s) 796
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 4 cut(s) 161, 250, 496, 1001
BfaI CTAG 3 cut(s) 147, 324, 333
BfmI CTRYAG 3 cut(s) 867, 1424, 1461
BfoI RGCGCY 2 cut(s) 139, 153
BfuAI ACCTGC 1 cut(s) 876
BglI GCCNNNNNGGC 1 cut(s) 194
BisI GCNGC 5 cut(s) 81, 186, 264, 1000, 1464
BlsI GCNGC 5 cut(s) 82, 187, 265, 1001, 1465
Bme1390I CCNGG 5 cut(s) 18, 307, 439, 955, 996
BmeRI GACNNNNNGTC 1 cut(s) 949
BmgT120I GGNCC 4 cut(s) 565, 566, 840, 1059
BmiI GGNNCC 5 cut(s) 15, 22, 137, 540, 567
BmrFI CCNGG 5 cut(s) 18, 307, 439, 955, 996
BmtI GCTAGC 1 cut(s) 327
BoxI GACNNNNGTC 2 cut(s) 314, 684
BpiI GAAGAC 1 cut(s) 739
BpuMI CCSGG 1 cut(s) 18
BsaAI YACGTR 1 cut(s) 421
BsaHI GRCGYC 1 cut(s) 136
BsaI GGTCTC 3 cut(s) 161, 496, 1001
BsaJI CCNNGG 2 cut(s) 294, 808
BsaWI WCCGGW 1 cut(s) 210
BsaXI ACNNNNNCTCC 6 cut(s) 213, 243, 976, 1006, 1104, 1134
Bsc4I CCNNNNNNNGG 6 cut(s) 444, 501, 548, 626, 814, 954
Bse1I ACTGG 2 cut(s) 780, 987
Bse3DI GCAATG 2 cut(s) 569, 1009
BseBI CCWGG 4 cut(s) 307, 439, 955, 996
BseDI CCNNGG 2 cut(s) 294, 808
BseGI GGATG 1 cut(s) 1127
BseLI CCNNNNNNNGG 6 cut(s) 444, 501, 548, 626, 814, 954
BseMI GCAATG 2 cut(s) 569, 1009
BseNI ACTGG 2 cut(s) 780, 987
BseRI GAGGAG 2 cut(s) 26, 113
BseSI GKGCMC 1 cut(s) 569
BseXI GCAGC 4 cut(s) 92, 172, 1011, 1475
BseYI CCCAGC 1 cut(s) 905
BsgI GTGCAG 1 cut(s) 774
Bsh1236I CGCG 2 cut(s) 1192, 1218
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 6 cut(s) 53, 305, 567, 842, 849, 1061
BshNI GGYRCC 2 cut(s) 20, 135
BsiEI CGRYCG 1 cut(s) 9
BsiHKAI GWGCWC 1 cut(s) 1375
BsiSI CCGG 3 cut(s) 18, 84, 211
BslFI GGGAC 4 cut(s) 17, 55, 680, 1188
BslI CCNNNNNNNGG 6 cut(s) 444, 501, 548, 626, 814, 954
BsmAI GTCTC 4 cut(s) 161, 250, 496, 1001
BsmFI GGGAC 4 cut(s) 17, 55, 680, 1188
BsnI GGCC 6 cut(s) 53, 305, 567, 842, 849, 1061
Bso31I GGTCTC 3 cut(s) 161, 496, 1001
Bsp120I GGGCCC 1 cut(s) 565
Bsp1286I GDGCHC 6 cut(s) 18, 78, 385, 569, 883, 1375
Bsp143I GATC 5 cut(s) 6, 788, 796, 1151, 1238
BspANI GGCC 6 cut(s) 53, 305, 567, 842, 849, 1061
BspFNI CGCG 2 cut(s) 1192, 1218
BspHI TCATGA 1 cut(s) 429
BspLI GGNNCC 5 cut(s) 15, 22, 137, 540, 567
BspMAI CTGCAG 3 cut(s) 871, 1428, 1465
BspMI ACCTGC 1 cut(s) 876
BspOI GCTAGC 1 cut(s) 327
BspPI GGATC 1 cut(s) 796
BspT107I GGYRCC 2 cut(s) 20, 135
BspTNI GGTCTC 3 cut(s) 161, 496, 1001
BsrDI GCAATG 2 cut(s) 569, 1009
BsrI ACTGG 2 cut(s) 780, 987
BssECI CCNNGG 2 cut(s) 294, 808
BssMI GATC 5 cut(s) 6, 788, 796, 1151, 1238
BssNAI GTATAC 1 cut(s) 1364
BssNI GRCGYC 1 cut(s) 136
BssT1I CCWWGG 2 cut(s) 294, 808
Bst1107I GTATAC 1 cut(s) 1364
Bst2UI CCWGG 4 cut(s) 307, 439, 955, 996
Bst4CI ACNGT 3 cut(s) 206, 728, 1231
Bst6I CTCTTC 1 cut(s) 729
BstACI GRCGYC 1 cut(s) 136
BstBAI YACGTR 1 cut(s) 421
BstC8I GCNNGC 4 cut(s) 78, 325, 883, 1190
BstENI CCTNNNNNAGG 1 cut(s) 812
BstF5I GGATG 1 cut(s) 1127
BstFNI CGCG 2 cut(s) 1192, 1218
BstH2I RGCGCY 2 cut(s) 139, 153
BstHHI GCGC 2 cut(s) 138, 152
BstKTI GATC 5 cut(s) 9, 791, 799, 1154, 1241
BstMAI GTCTC 4 cut(s) 161, 250, 496, 1001
BstMBI GATC 5 cut(s) 6, 788, 796, 1151, 1238
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 7 cut(s) 194, 311, 320, 329, 338, 1058, 1189
BstNI CCWGG 4 cut(s) 307, 439, 955, 996
BstPAI GACNNNNGTC 2 cut(s) 314, 684
BstSCI CCNGG 5 cut(s) 16, 305, 437, 953, 994
BstSFI CTRYAG 3 cut(s) 867, 1424, 1461
BstSLI GKGCMC 1 cut(s) 569
BstUI CGCG 2 cut(s) 1192, 1218
BstV1I GCAGC 4 cut(s) 92, 172, 1011, 1475
BstV2I GAAGAC 1 cut(s) 739
BstZ17I GTATAC 1 cut(s) 1364
BsuRI GGCC 6 cut(s) 53, 305, 567, 842, 849, 1061
BtgZI GCGATG 2 cut(s) 281, 1033
BtsCI GGATG 1 cut(s) 1127
BtsI GCAGTG 2 cut(s) 170, 1125
BtsIMutI CAGTG 3 cut(s) 170, 603, 1125
BveI ACCTGC 1 cut(s) 876
Cac8I GCNNGC 4 cut(s) 78, 325, 883, 1190
CciI TCATGA 1 cut(s) 429
CfoI GCGC 2 cut(s) 138, 152
Cfr13I GGNCC 4 cut(s) 565, 566, 840, 1059
CseI GACGC 3 cut(s) 749, 1207, 1319
Csp6I GTAC 1 cut(s) 981
CviAII CATG 4 cut(s) 319, 430, 476, 543
CviQI GTAC 1 cut(s) 981
DinI GGCGCC 1 cut(s) 137
DpnI GATC 5 cut(s) 8, 790, 798, 1153, 1240
DpnII GATC 5 cut(s) 6, 788, 796, 1151, 1238
DraIII CACNNNGTG 1 cut(s) 242
DrdI GACNNNNNNGTC 1 cut(s) 1217
DriI GACNNNNNGTC 1 cut(s) 949
DseDI GACNNNNNNGTC 1 cut(s) 1217
Eam1104I CTCTTC 1 cut(s) 729
Eam1105I GACNNNNNGTC 1 cut(s) 949
EarI CTCTTC 1 cut(s) 729
EciI GGCGGA 1 cut(s) 1008
Ecl136II GAGCTC 1 cut(s) 1373
Eco130I CCWWGG 2 cut(s) 294, 808
Eco24I GRGCYC 6 cut(s) 18, 78, 385, 569, 883, 1375
Eco31I GGTCTC 3 cut(s) 161, 496, 1001
Eco47III AGCGCT 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 1373
Eco57I CTGAAG 1 cut(s) 753
Eco72I CACGTG 1 cut(s) 421
EcoICRI GAGCTC 1 cut(s) 1373
EcoNI CCTNNNNNAGG 1 cut(s) 812
EcoO109I RGGNCCY 1 cut(s) 565
EcoRII CCWGG 4 cut(s) 305, 437, 953, 994
EcoT14I CCWWGG 2 cut(s) 294, 808
EcoT38I GRGCYC 6 cut(s) 18, 78, 385, 569, 883, 1375
EgeI GGCGCC 1 cut(s) 137
EheI GGCGCC 1 cut(s) 137
ErhI CCWWGG 2 cut(s) 294, 808
FaeI CATG 4 cut(s) 322, 433, 479, 546
FalI AAGNNNNNCTT 2 cut(s) 1325, 1357
FaqI GGGAC 4 cut(s) 17, 55, 680, 1188
FatI CATG 4 cut(s) 318, 429, 475, 542
FauI CCCGC 1 cut(s) 480
FauNDI CATATG 2 cut(s) 793, 1278
FbaI TGATCA 1 cut(s) 796
FblI GTMKAC 2 cut(s) 890, 1363
Fnu4HI GCNGC 5 cut(s) 81, 186, 264, 1000, 1464
FokI GGATG 1 cut(s) 1114
FriOI GRGCYC 6 cut(s) 18, 78, 385, 569, 883, 1375
Fsp4HI GCNGC 5 cut(s) 81, 186, 264, 1000, 1464
FspBI CTAG 3 cut(s) 147, 324, 333
GlaI GCGC 2 cut(s) 137, 151
GluI GCNGC 5 cut(s) 81, 186, 264, 1000, 1464
GsaI CCCAGC 1 cut(s) 909
HaeII RGCGCY 2 cut(s) 139, 153
HaeIII GGCC 6 cut(s) 53, 305, 567, 842, 849, 1061
HapII CCGG 3 cut(s) 18, 84, 211
HgaI GACGC 3 cut(s) 749, 1207, 1319
HhaI GCGC 2 cut(s) 138, 152
Hin1I GRCGYC 1 cut(s) 136
Hin1II CATG 4 cut(s) 322, 433, 479, 546
Hin6I GCGC 2 cut(s) 136, 150
HinP1I GCGC 2 cut(s) 136, 150
HinfI GANTC 4 cut(s) 965, 1067, 1147, 1198
HpaII CCGG 3 cut(s) 18, 84, 211
HphI GGTGA 2 cut(s) 1018, 1233
Hpy166II GTNNAC 2 cut(s) 891, 1364
Hpy188I TCNGA 2 cut(s) 913, 964
Hpy188III TCNNGA 4 cut(s) 430, 677, 1131, 1263
Hpy8I GTNNAC 2 cut(s) 891, 1364
Hpy99I CGWCG 2 cut(s) 67, 1067
HpyAV CCTTC 3 cut(s) 372, 1025, 1033
HpyCH4III ACNGT 3 cut(s) 206, 728, 1231
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 8 cut(s) 236, 499, 755, 869, 1188, 1330, 1426, 1463
HpyF10VI GCNNNNNNNGC 7 cut(s) 194, 311, 320, 329, 338, 1058, 1189
HpySE526I ACGT 1 cut(s) 420
Hsp92I GRCGYC 1 cut(s) 136
Hsp92II CATG 4 cut(s) 322, 433, 479, 546
HspAI GCGC 2 cut(s) 136, 150
KasI GGCGCC 1 cut(s) 135
Ksp22I TGATCA 1 cut(s) 796
Kzo9I GATC 5 cut(s) 6, 788, 796, 1151, 1238
LmnI GCTCC 3 cut(s) 13, 914, 1370
Lsp1109I GCAGC 4 cut(s) 92, 172, 1011, 1475
MaeI CTAG 3 cut(s) 147, 324, 333
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 3 cut(s) 601, 862, 1233
MalI GATC 5 cut(s) 8, 790, 798, 1153, 1240
MboI GATC 5 cut(s) 6, 788, 796, 1151, 1238
MboII GAAGA 3 cut(s) 739, 746, 938
MhlI GDGCHC 6 cut(s) 18, 78, 385, 569, 883, 1375
MluCI AATT 5 cut(s) 280, 508, 1271, 1383, 1469
MluI ACGCGT 1 cut(s) 1216
Mly113I GGCGCC 1 cut(s) 136
MlyI GAGTC 1 cut(s) 959
MmeI TCCRAC 3 cut(s) 83, 987, 1090
MroXI GAANNNNTTC 1 cut(s) 1272
MseI TTAA 3 cut(s) 300, 1349, 1437
MspA1I CMGCKG 1 cut(s) 314
MspI CCGG 3 cut(s) 18, 84, 211
MspR9I CCNGG 5 cut(s) 18, 307, 439, 955, 996
MvaI CCWGG 4 cut(s) 307, 439, 955, 996
MvnI CGCG 2 cut(s) 1192, 1218
MwoI GCNNNNNNNGC 7 cut(s) 194, 311, 320, 329, 338, 1058, 1189
NarI GGCGCC 1 cut(s) 136
NciI CCSGG 1 cut(s) 18
NdeI CATATG 2 cut(s) 793, 1278
NdeII GATC 5 cut(s) 6, 788, 796, 1151, 1238
NheI GCTAGC 1 cut(s) 323
NlaIII CATG 4 cut(s) 322, 433, 479, 546
NlaIV GGNNCC 5 cut(s) 15, 22, 137, 540, 567
NmuCI GTSAC 1 cut(s) 601
PagI TCATGA 1 cut(s) 429
PdmI GAANNNNTTC 1 cut(s) 1272
PfeI GAWTC 3 cut(s) 1067, 1147, 1198
PflFI GACNNNGTC 1 cut(s) 743
PflMI CCANNNNNTGG 1 cut(s) 548
PkrI GCNGC 5 cut(s) 82, 187, 265, 1001, 1465
Ple19I CGATCG 1 cut(s) 9
PleI GAGTC 1 cut(s) 959
PluTI GGCGCC 1 cut(s) 139
PmaCI CACGTG 1 cut(s) 421
PmlI CACGTG 1 cut(s) 421
PpsI GAGTC 1 cut(s) 959
Ppu21I YACGTR 1 cut(s) 421
PshAI GACNNNNGTC 2 cut(s) 314, 684
Psp124BI GAGCTC 1 cut(s) 1375
Psp6I CCWGG 4 cut(s) 305, 437, 953, 994
PspCI CACGTG 1 cut(s) 421
PspFI CCCAGC 1 cut(s) 905
PspGI CCWGG 4 cut(s) 305, 437, 953, 994
PspN4I GGNNCC 5 cut(s) 15, 22, 137, 540, 567
PspOMI GGGCCC 1 cut(s) 565
PspPI GGNCC 4 cut(s) 565, 566, 840, 1059
PsrI GAACNNNNNNTAC 2 cut(s) 973, 1005
PstI CTGCAG 3 cut(s) 871, 1428, 1465
PsyI GACNNNGTC 1 cut(s) 743
PvuI CGATCG 1 cut(s) 9
RsaI GTAC 1 cut(s) 982
RsaNI GTAC 1 cut(s) 981
SacI GAGCTC 1 cut(s) 1375
SaqAI TTAA 3 cut(s) 300, 1349, 1437
SatI GCNGC 5 cut(s) 81, 186, 264, 1000, 1464
Sau3AI GATC 5 cut(s) 6, 788, 796, 1151, 1238
Sau96I GGNCC 4 cut(s) 565, 566, 840, 1059
SchI GAGTC 1 cut(s) 959
ScrFI CCNGG 5 cut(s) 18, 307, 439, 955, 996
SduI GDGCHC 6 cut(s) 18, 78, 385, 569, 883, 1375
SfcI CTRYAG 3 cut(s) 867, 1424, 1461
SfoI GGCGCC 1 cut(s) 137
Sse9I AATT 5 cut(s) 280, 508, 1271, 1383, 1469
SspDI GGCGCC 1 cut(s) 135
SspMI CTAG 3 cut(s) 147, 324, 333
SstI GAGCTC 1 cut(s) 1375
StyD4I CCNGG 5 cut(s) 16, 305, 437, 953, 994
StyI CCWWGG 2 cut(s) 294, 808
TaaI ACNGT 3 cut(s) 206, 728, 1231
TaiI ACGT 1 cut(s) 423
TaqI TCGA 5 cut(s) 5, 9, 644, 1065, 1150
TasI AATT 5 cut(s) 280, 508, 1271, 1383, 1469
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 3 cut(s) 1067, 1147, 1198
Tru1I TTAA 3 cut(s) 300, 1349, 1437
Tru9I TTAA 3 cut(s) 300, 1349, 1437
TscAI CASTG 3 cut(s) 170, 610, 1125
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 4 cut(s) 80, 185, 999, 1463
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 6 cut(s) 418, 464, 939, 1036, 1265, 1395
TspGWI ACGGA 2 cut(s) 484, 1431
TspRI CASTG 3 cut(s) 170, 610, 1125
Tth111I GACNNNGTC 1 cut(s) 743
Van91I CCANNNNNTGG 1 cut(s) 548
XagI CCTNNNNNAGG 1 cut(s) 812
XcmI CCANNNNNNNNNTGG 2 cut(s) 850, 1364
XmiI GTMKAC 2 cut(s) 890, 1363
XmnI GAANNNNTTC 1 cut(s) 1272
XspI CTAG 3 cut(s) 147, 324, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.