Rmu_sc0004892.1_g000011

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004892.1
OG0002948
Physical Location & Seq
Reverse (-)
30821 .. 34608
3788 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1035 bp
atgtttaacagggaatttttatttcttaatgcagaactgagattcaagcacaagattcatagccatagccccgtctacaatcacactcttgctacaatattggttcggtatgcttctacggtgtatttgtcagatttgacagagctgttttcttggacatgcgacgaatgcaatggcttgatcaaggattttcaaatgatagagctgattgttgacatccagcattgcttacaggcatatgttggagtcgcaacagatcctaatgctattataattgcctttagaggaactcaggaacacagcatacagaattggattgaagacttattttggaagcaacttgatatagattaccccggcatgcctgatgcaatggtgcaccatgggttttacagcgcttaccacaacaccaccatacgccctggaattctaaatgctgttgcaagagccaaagagttttatggagatattgggatcatagtgacagggcattcaatgggaggggcaatggcttcattttgtgctcttgatttaagggtcaatcaaaaagagaacaatgttcaagttatgacatttggacaacctcgtatcggtaatgcaatctttgctacttactatagcaaacttgtgccaaaaagtatacgcataacaaatggaaatgatgttgtgcctcatttgcctccatactatactcactttcctcagaagacataccaccacttcccaagagaggtgtggctttataacattggattgggaagtcttgtttataaggttgagaaaatatgtgatgcctctggcgaggacccaacatgcagcaggtcagtgaaagggaatagcatttcagaccatttagtttattttggtgtcgatttaatggccaagacatggagaccgtgcaaaattgtgatgggtcctggtttactggagcatggaagaacagatctggaaggaaattttgtactgtccagagatcttgcaactcctgttctcaaattgaaaacacggccagacgcccggggaaagcctctgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

344

Amino Acids

38.97

Weight (kDa)

7.26

Isoelectric Point (pI)

25.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 91 - 229 3.7e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 272, 744, 771
Acc36I ACCTGC 1 cut(s) 810
AccB7I CCANNNNNTGG 1 cut(s) 888
AccI GTMKAC 2 cut(s) 75, 640
AclWI GGATC 2 cut(s) 251, 482
AcoI YGGCCR 2 cut(s) 879, 1007
AcsI RAATTY 3 cut(s) 14, 426, 955
AcyI GRCGYC 1 cut(s) 1014
AfaI GTAC 1 cut(s) 963
AfeI AGCGCT 1 cut(s) 397
AfiI CCNNNNNNNGG 3 cut(s) 590, 730, 888
AgsI TTSAA 6 cut(s) 46, 194, 320, 495, 563, 1000
AjnI CCWGG 2 cut(s) 421, 916
AluBI AGCT 2 cut(s) 145, 205
AluI AGCT 2 cut(s) 145, 205
Alw21I GWGCWC 2 cut(s) 381, 526
Alw26I GTCTC 1 cut(s) 886
Alw44I GTGCAC 1 cut(s) 377
AlwI GGATC 2 cut(s) 251, 482
Ama87I CYCGRG 1 cut(s) 1017
Aor51HI AGCGCT 1 cut(s) 397
AoxI GGCC 2 cut(s) 879, 1007
ApaLI GTGCAC 1 cut(s) 377
ApeKI GCWGC 1 cut(s) 816
ApoI RAATTY 3 cut(s) 14, 426, 955
AspLEI GCGC 1 cut(s) 398
AspS9I GGNCC 2 cut(s) 805, 914
AsuC2I CCSGG 3 cut(s) 357, 1018, 1019
AvaI CYCGRG 1 cut(s) 1017
AvaII GGWCC 2 cut(s) 805, 914
BaeGI GKGCMC 1 cut(s) 381
BalI TGGCCA 1 cut(s) 881
BbsI GAAGAC 2 cut(s) 327, 713
Bbv12I GWGCWC 2 cut(s) 381, 526
BbvI GCAGC 1 cut(s) 828
BccI CCATC 1 cut(s) 904
BceAI ACGGC 1 cut(s) 1022
BciT130I CCWGG 2 cut(s) 423, 918
BclI TGATCA 1 cut(s) 180
BcnI CCSGG 3 cut(s) 357, 1018, 1019
BcoDI GTCTC 1 cut(s) 886
BfmI CTRYAG 2 cut(s) 616, 1031
BfoI RGCGCY 1 cut(s) 399
BfuAI ACCTGC 1 cut(s) 810
BglII AGATCT 2 cut(s) 943, 973
BisI GCNGC 1 cut(s) 817
BlsI GCNGC 1 cut(s) 818
Bme1390I CCNGG 5 cut(s) 357, 423, 918, 1018, 1019
Bme18I GGWCC 2 cut(s) 805, 914
BmeT110I CYCGRG 1 cut(s) 1017
BmgT120I GGNCC 2 cut(s) 805, 914
BmiI GGNNCC 2 cut(s) 807, 915
BmrFI CCNGG 5 cut(s) 357, 423, 918, 1018, 1019
BmsI GCATC 2 cut(s) 358, 781
BpiI GAAGAC 2 cut(s) 327, 713
BpmI CTGGAG 1 cut(s) 947
BpuMI CCSGG 3 cut(s) 357, 1018, 1019
BsaHI GRCGYC 1 cut(s) 1014
BsaI GGTCTC 1 cut(s) 886
BsaJI CCNNGG 5 cut(s) 355, 382, 421, 1017, 1018
Bsc4I CCNNNNNNNGG 3 cut(s) 590, 730, 888
Bse1I ACTGG 1 cut(s) 930
Bse3DI GCAATG 4 cut(s) 178, 223, 378, 513
BseBI CCWGG 2 cut(s) 423, 918
BseDI CCNNGG 5 cut(s) 355, 382, 421, 1017, 1018
BseGI GGATG 1 cut(s) 216
BseLI CCNNNNNNNGG 3 cut(s) 590, 730, 888
BseMI GCAATG 4 cut(s) 178, 223, 378, 513
BseMII CTCAG 3 cut(s) 29, 305, 716
BseNI ACTGG 1 cut(s) 930
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 1 cut(s) 828
BshFI GGCC 2 cut(s) 881, 1009
BsiHKAI GWGCWC 2 cut(s) 381, 526
BsiHKCI CYCGRG 1 cut(s) 1017
BsiSI CCGG 2 cut(s) 357, 1018
BslI CCNNNNNNNGG 3 cut(s) 590, 730, 888
BsmAI GTCTC 1 cut(s) 886
BsmI GAATGC 2 cut(s) 173, 490
BsnI GGCC 2 cut(s) 881, 1009
Bso31I GGTCTC 1 cut(s) 886
BsoBI CYCGRG 1 cut(s) 1017
Bsp1286I GDGCHC 2 cut(s) 381, 526
Bsp143I GATC 5 cut(s) 180, 256, 474, 943, 973
Bsp19I CCATGG 1 cut(s) 382
BspANI GGCC 2 cut(s) 881, 1009
BspCNI CTCAG 3 cut(s) 30, 304, 715
BspLI GGNNCC 2 cut(s) 807, 915
BspMI ACCTGC 1 cut(s) 810
BspPI GGATC 2 cut(s) 251, 482
BspTNI GGTCTC 1 cut(s) 886
BsrDI GCAATG 4 cut(s) 178, 223, 378, 513
BsrI ACTGG 1 cut(s) 930
BssECI CCNNGG 5 cut(s) 355, 382, 421, 1017, 1018
BssMI GATC 5 cut(s) 180, 256, 474, 943, 973
BssNAI GTATAC 1 cut(s) 641
BssNI GRCGYC 1 cut(s) 1014
BssT1I CCWWGG 1 cut(s) 382
Bst1107I GTATAC 1 cut(s) 641
Bst2UI CCWGG 2 cut(s) 423, 918
Bst4CI ACNGT 3 cut(s) 121, 897, 966
BstACI GRCGYC 1 cut(s) 1014
BstAPI GCANNNNNTGC 1 cut(s) 605
BstC8I GCNNGC 1 cut(s) 362
BstDEI CTNAG 3 cut(s) 38, 291, 702
BstDSI CCRYGG 1 cut(s) 382
BstF5I GGATG 1 cut(s) 216
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 5 cut(s) 183, 259, 477, 946, 976
BstMAI GTCTC 1 cut(s) 886
BstMBI GATC 5 cut(s) 180, 256, 474, 943, 973
BstMWI GCNNNNNNNGC 3 cut(s) 168, 605, 676
BstNI CCWGG 2 cut(s) 423, 918
BstNSI RCATGY 3 cut(s) 162, 364, 816
BstSCI CCNGG 5 cut(s) 355, 421, 916, 1016, 1017
BstSFI CTRYAG 2 cut(s) 616, 1031
BstSLI GKGCMC 1 cut(s) 381
BstV1I GCAGC 1 cut(s) 828
BstV2I GAAGAC 2 cut(s) 327, 713
BstX2I RGATCY 3 cut(s) 256, 943, 973
BstYI RGATCY 3 cut(s) 256, 943, 973
BstZ17I GTATAC 1 cut(s) 641
BsuRI GGCC 2 cut(s) 881, 1009
BtgI CCRYGG 1 cut(s) 382
BtsCI GGATG 1 cut(s) 216
BtsIMutI CAGTG 1 cut(s) 831
BveI ACCTGC 1 cut(s) 810
Cac8I GCNNGC 1 cut(s) 362
CfoI GCGC 1 cut(s) 398
Cfr13I GGNCC 2 cut(s) 805, 914
Cfr9I CCCGGG 1 cut(s) 1017
CseI GACGC 1 cut(s) 1022
Csp6I GTAC 1 cut(s) 962
CviAII CATG 6 cut(s) 159, 361, 383, 813, 888, 932
CviQI GTAC 1 cut(s) 962
DdeI CTNAG 3 cut(s) 38, 291, 702
DpnI GATC 5 cut(s) 182, 258, 476, 945, 975
DpnII GATC 5 cut(s) 180, 256, 474, 943, 973
EaeI YGGCCR 2 cut(s) 879, 1007
Eco130I CCWWGG 1 cut(s) 382
Eco31I GGTCTC 1 cut(s) 886
Eco47I GGWCC 2 cut(s) 805, 914
Eco47III AGCGCT 1 cut(s) 397
Eco88I CYCGRG 1 cut(s) 1017
EcoO109I RGGNCCY 2 cut(s) 805, 914
EcoRI GAATTC 1 cut(s) 426
EcoRII CCWGG 2 cut(s) 421, 916
EcoT14I CCWWGG 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 382
FaeI CATG 6 cut(s) 162, 364, 386, 816, 891, 935
FatI CATG 6 cut(s) 158, 360, 382, 812, 887, 931
FauNDI CATATG 1 cut(s) 238
FbaI TGATCA 1 cut(s) 180
FblI GTMKAC 2 cut(s) 75, 640
Fnu4HI GCNGC 1 cut(s) 817
FokI GGATG 1 cut(s) 203
Fsp4HI GCNGC 1 cut(s) 817
GlaI GCGC 1 cut(s) 397
GluI GCNGC 1 cut(s) 817
GsuI CTGGAG 1 cut(s) 947
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 2 cut(s) 881, 1009
HapII CCGG 2 cut(s) 357, 1018
HgaI GACGC 1 cut(s) 1022
HhaI GCGC 1 cut(s) 398
Hin1I GRCGYC 1 cut(s) 1014
Hin1II CATG 6 cut(s) 162, 364, 386, 816, 891, 935
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 3 cut(s) 42, 55, 246
HpaII CCGG 2 cut(s) 357, 1018
Hpy166II GTNNAC 5 cut(s) 76, 214, 379, 641, 923
Hpy188I TCNGA 3 cut(s) 133, 705, 847
Hpy188III TCNNGA 4 cut(s) 293, 527, 947, 969
Hpy8I GTNNAC 5 cut(s) 76, 214, 379, 641, 923
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 1 cut(s) 944
HpyCH4III ACNGT 3 cut(s) 121, 897, 966
HpyCH4V TGCA 9 cut(s) 32, 171, 371, 379, 443, 599, 816, 900, 980
HpyF10VI GCNNNNNNNGC 3 cut(s) 168, 605, 676
HpyF3I CTNAG 3 cut(s) 38, 291, 702
Hsp92I GRCGYC 1 cut(s) 1014
Hsp92II CATG 6 cut(s) 162, 364, 386, 816, 891, 935
HspAI GCGC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 5 cut(s) 180, 256, 474, 943, 973
LmnI GCTCC 1 cut(s) 928
Lsp1109I GCAGC 1 cut(s) 828
LweI GCATC 2 cut(s) 358, 781
MaeIII GTNAC 1 cut(s) 481
MalI GATC 5 cut(s) 182, 258, 476, 945, 975
MboI GATC 5 cut(s) 180, 256, 474, 943, 973
MboII GAAGA 3 cut(s) 332, 718, 948
MflI RGATCY 3 cut(s) 256, 943, 973
MhlI GDGCHC 2 cut(s) 381, 526
MlsI TGGCCA 1 cut(s) 881
MluCI AATT 7 cut(s) 14, 273, 310, 426, 903, 955, 995
MluNI TGGCCA 1 cut(s) 881
MlyI GAGTC 1 cut(s) 255
MmeI TCCRAC 1 cut(s) 223
MnlI CCTC 9 cut(s) 278, 494, 594, 681, 690, 711, 724, 796, 805
Mox20I TGGCCA 1 cut(s) 881
MscI TGGCCA 1 cut(s) 881
MseI TTAA 4 cut(s) 6, 27, 533, 875
Msp20I TGGCCA 1 cut(s) 881
MspI CCGG 2 cut(s) 357, 1018
MspR9I CCNGG 5 cut(s) 357, 423, 918, 1018, 1019
Mva1269I GAATGC 2 cut(s) 173, 490
MvaI CCWGG 2 cut(s) 423, 918
MwoI GCNNNNNNNGC 3 cut(s) 168, 605, 676
NciI CCSGG 3 cut(s) 357, 1018, 1019
NcoI CCATGG 1 cut(s) 382
NdeI CATATG 1 cut(s) 238
NdeII GATC 5 cut(s) 180, 256, 474, 943, 973
NlaIII CATG 6 cut(s) 162, 364, 386, 816, 891, 935
NlaIV GGNNCC 2 cut(s) 807, 915
NmuCI GTSAC 1 cut(s) 481
NspI RCATGY 3 cut(s) 162, 364, 816
PaeI GCATGC 1 cut(s) 364
PctI GAATGC 2 cut(s) 173, 490
PfeI GAWTC 2 cut(s) 42, 55
PflMI CCANNNNNTGG 1 cut(s) 888
PkrI GCNGC 1 cut(s) 818
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PpuMI RGGWCCY 2 cut(s) 805, 914
PsiI TTATAA 3 cut(s) 272, 744, 771
Psp5II RGGWCCY 2 cut(s) 805, 914
Psp6I CCWGG 2 cut(s) 421, 916
PspGI CCWGG 2 cut(s) 421, 916
PspN4I GGNNCC 2 cut(s) 807, 915
PspPI GGNCC 2 cut(s) 805, 914
PspPPI RGGWCCY 2 cut(s) 805, 914
PsrI GAACNNNNNNTAC 2 cut(s) 288, 320
PsuI RGATCY 3 cut(s) 256, 943, 973
RsaI GTAC 1 cut(s) 963
RsaNI GTAC 1 cut(s) 962
SaqAI TTAA 4 cut(s) 6, 27, 533, 875
SatI GCNGC 1 cut(s) 817
Sau3AI GATC 5 cut(s) 180, 256, 474, 943, 973
Sau96I GGNCC 2 cut(s) 805, 914
SchI GAGTC 1 cut(s) 255
ScrFI CCNGG 5 cut(s) 357, 423, 918, 1018, 1019
SduI GDGCHC 2 cut(s) 381, 526
SetI ASST 6 cut(s) 147, 207, 586, 735, 777, 824
SfaNI GCATC 2 cut(s) 358, 781
SfcI CTRYAG 2 cut(s) 616, 1031
SinI GGWCC 2 cut(s) 805, 914
SmaI CCCGGG 1 cut(s) 1019
SphI GCATGC 1 cut(s) 364
Sse9I AATT 7 cut(s) 14, 273, 310, 426, 903, 955, 995
SspI AATATT 1 cut(s) 99
StyD4I CCNGG 5 cut(s) 355, 421, 916, 1016, 1017
StyI CCWWGG 1 cut(s) 382
TaaI ACNGT 3 cut(s) 121, 897, 966
TaqI TCGA 1 cut(s) 870
TasI AATT 7 cut(s) 14, 273, 310, 426, 903, 955, 995
TatI WGTACW 1 cut(s) 961
TfiI GAWTC 2 cut(s) 42, 55
Tru1I TTAA 4 cut(s) 6, 27, 533, 875
Tru9I TTAA 4 cut(s) 6, 27, 533, 875
TscAI CASTG 1 cut(s) 831
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 1 cut(s) 816
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 2 cut(s) 47, 504
TspMI CCCGGG 1 cut(s) 1017
TspRI CASTG 1 cut(s) 831
Van91I CCANNNNNTGG 1 cut(s) 888
VneI GTGCAC 1 cut(s) 377
VpaK11BI GGWCC 2 cut(s) 805, 914
XapI RAATTY 3 cut(s) 14, 426, 955
XceI RCATGY 3 cut(s) 162, 364, 816
XcmI CCANNNNNNNNNTGG 1 cut(s) 732
XmaI CCCGGG 1 cut(s) 1017
XmiI GTMKAC 2 cut(s) 75, 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.