Rmu_sc0004976.1_g000015

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004976.1
OG0006910
Physical Location & Seq
Forward (+)
60634 .. 61128
495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004976.1_g000015.1.cds

Sequence Viewer

Length: 495 bp
atggttcgcacaaagcaaacaactcgacttggcggacatcgccgcctgcctcctccagaggaaggccgtcaggccgctgtccgagccgggattctccatcccggcatgcagcgtgaacgaagtcggagccctcctcttcgctcctcctcttctggtacctctgctgctactcctcctcctgtctctttggatagtctccgtgaccagatcctggtggctgactggagaatttcctgcctcactacggacatggatgacatgcattcccttctggttcgtactcgtcatgctctgaccaccctcacacaggagatccggaacttgcagcgtcaccctcccgggtttgacgctcctggtccctccacggctgttcccgtagccgtcgcccctgaggagagctccacttcgatggacagcgaggaatttcaggatcatgtcgtcaagcttatggaggaagtcgaagggcattcccattcaaagggggagaagaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

18.04

Weight (kDa)

7.11

Isoelectric Point (pI)

79.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 155
AccB1I GGYRCC 1 cut(s) 155
AccB7I CCANNNNNTGG 1 cut(s) 211
AccIII TCCGGA 1 cut(s) 315
AciI CCGC 3 cut(s) 33, 43, 75
AclWI GGATC 3 cut(s) 202, 307, 438
AcsI RAATTY 2 cut(s) 228, 422
AfaI GTAC 2 cut(s) 157, 280
AfiI CCNNNNNNNGG 5 cut(s) 62, 211, 244, 307, 478
AgsI TTSAA 1 cut(s) 477
AjnI CCWGG 2 cut(s) 210, 352
AluBI AGCT 2 cut(s) 399, 445
AluI AGCT 2 cut(s) 399, 445
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 2 cut(s) 187, 200
AlwI GGATC 3 cut(s) 202, 307, 438
AlwNI CAGNNNCTG 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 338
Aor13HI TCCGGA 1 cut(s) 315
AoxI GGCC 2 cut(s) 64, 72
ApeKI GCWGC 3 cut(s) 109, 164, 325
ApoI RAATTY 2 cut(s) 228, 422
Asp718I GGTACC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 356
AsuC2I CCSGG 4 cut(s) 88, 102, 339, 340
AsuHPI GGTGA 1 cut(s) 323
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 1 cut(s) 356
AxyI CCTNAGG 1 cut(s) 390
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 2 cut(s) 131, 401
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 3 cut(s) 121, 151, 337
BccI CCATC 2 cut(s) 105, 403
BceAI ACGGC 3 cut(s) 51, 365, 381
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 2 cut(s) 212, 354
BcnI CCSGG 4 cut(s) 88, 102, 339, 340
BcoDI GTCTC 2 cut(s) 187, 200
BisI GCNGC 5 cut(s) 43, 75, 110, 165, 326
BlsI GCNGC 5 cut(s) 44, 76, 111, 166, 327
Bme1390I CCNGG 6 cut(s) 88, 102, 212, 339, 340, 354
Bme18I GGWCC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 3 cut(s) 128, 157, 358
BmrFI CCNGG 6 cut(s) 88, 102, 212, 339, 340, 354
BplI GAGNNNNNCTC 4 cut(s) 118, 150, 383, 415
BpmI CTGGAG 2 cut(s) 39, 244
BpuMI CCSGG 4 cut(s) 88, 102, 339, 340
BsaJI CCNNGG 2 cut(s) 338, 363
BsaWI WCCGGW 1 cut(s) 315
BsaXI ACNNNNNCTCC 3 cut(s) 160, 190, 476
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 211, 244, 307, 478
Bse1I ACTGG 1 cut(s) 227
Bse21I CCTNAGG 1 cut(s) 390
BseAI TCCGGA 1 cut(s) 315
BseBI CCWGG 2 cut(s) 212, 354
BseDI CCNNGG 2 cut(s) 338, 363
BseGI GGATG 2 cut(s) 97, 259
BseLI CCNNNNNNNGG 5 cut(s) 62, 211, 244, 307, 478
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 7 cut(s) 42, 123, 133, 136, 162, 165, 407
BseXI GCAGC 3 cut(s) 121, 151, 337
BshFI GGCC 2 cut(s) 66, 74
BshNI GGYRCC 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 401
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 4 cut(s) 87, 102, 316, 339
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 5 cut(s) 62, 211, 244, 307, 478
BsmAI GTCTC 2 cut(s) 187, 200
BsmFI GGGAC 1 cut(s) 342
BsmI GAATGC 2 cut(s) 262, 466
BsnI GGCC 2 cut(s) 66, 74
BsoBI CYCGRG 1 cut(s) 338
Bsp1286I GDGCHC 2 cut(s) 131, 401
Bsp13I TCCGGA 1 cut(s) 315
Bsp143I GATC 3 cut(s) 207, 312, 430
BspACI CCGC 3 cut(s) 33, 43, 75
BspANI GGCC 2 cut(s) 66, 74
BspCNI CTCAG 1 cut(s) 382
BspEI TCCGGA 1 cut(s) 315
BspLI GGNNCC 3 cut(s) 128, 157, 358
BspPI GGATC 3 cut(s) 202, 307, 438
BspT107I GGYRCC 1 cut(s) 155
BsrI ACTGG 1 cut(s) 227
BssECI CCNNGG 2 cut(s) 338, 363
BssMI GATC 3 cut(s) 207, 312, 430
Bst2UI CCWGG 2 cut(s) 212, 354
Bst6I CTCTTC 2 cut(s) 141, 154
BstC8I GCNNGC 2 cut(s) 47, 107
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 1 cut(s) 363
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 2 cut(s) 97, 259
BstKTI GATC 3 cut(s) 210, 315, 433
BstMAI GTCTC 2 cut(s) 187, 200
BstMBI GATC 3 cut(s) 207, 312, 430
BstMWI GCNNNNNNNGC 2 cut(s) 39, 83
BstNI CCWGG 2 cut(s) 212, 354
BstNSI RCATGY 2 cut(s) 109, 262
BstSCI CCNGG 6 cut(s) 86, 100, 210, 337, 338, 352
BstV1I GCAGC 3 cut(s) 121, 151, 337
BstX2I RGATCY 2 cut(s) 207, 312
BstXI CCANNNNNNTGG 1 cut(s) 409
BstYI RGATCY 2 cut(s) 207, 312
Bsu36I CCTNAGG 1 cut(s) 390
BsuRI GGCC 2 cut(s) 66, 74
BtgI CCRYGG 1 cut(s) 363
BtgZI GCGATG 1 cut(s) 23
BtsCI GGATG 2 cut(s) 97, 259
Cac8I GCNNGC 2 cut(s) 47, 107
CaiI CAGNNNCTG 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 356
Cfr9I CCCGGG 1 cut(s) 338
CseI GACGC 2 cut(s) 317, 356
Csp6I GTAC 2 cut(s) 156, 279
CviAII CATG 5 cut(s) 106, 250, 259, 287, 434
CviJI RGCY 9 cut(s) 66, 74, 86, 129, 218, 368, 380, 399, 445
CviKI_1 RGCY 9 cut(s) 66, 74, 86, 129, 218, 368, 380, 399, 445
CviQI GTAC 2 cut(s) 156, 279
DdeI CTNAG 1 cut(s) 390
DpnI GATC 3 cut(s) 209, 314, 432
DpnII GATC 3 cut(s) 207, 312, 430
Eam1104I CTCTTC 2 cut(s) 141, 154
EarI CTCTTC 2 cut(s) 141, 154
EciI GGCGGA 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 399
Eco24I GRGCYC 2 cut(s) 131, 401
Eco47I GGWCC 1 cut(s) 356
Eco53kI GAGCTC 1 cut(s) 399
Eco81I CCTNAGG 1 cut(s) 390
Eco88I CYCGRG 1 cut(s) 338
EcoICRI GAGCTC 1 cut(s) 399
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoRII CCWGG 2 cut(s) 210, 352
EcoT22I ATGCAT 1 cut(s) 264
EcoT38I GRGCYC 2 cut(s) 131, 401
FaeI CATG 5 cut(s) 109, 253, 262, 290, 437
FaiI YATR 6 cut(s) 107, 251, 260, 288, 435, 449
FaqI GGGAC 1 cut(s) 342
FatI CATG 5 cut(s) 105, 249, 258, 286, 433
Fnu4HI GCNGC 5 cut(s) 43, 75, 110, 165, 326
FokI GGATG 2 cut(s) 84, 266
FriOI GRGCYC 2 cut(s) 131, 401
Fsp4HI GCNGC 5 cut(s) 43, 75, 110, 165, 326
GluI GCNGC 5 cut(s) 43, 75, 110, 165, 326
GsuI CTGGAG 2 cut(s) 39, 244
HaeIII GGCC 2 cut(s) 66, 74
HapII CCGG 4 cut(s) 87, 102, 316, 339
HgaI GACGC 2 cut(s) 317, 356
Hin1II CATG 5 cut(s) 109, 253, 262, 290, 437
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 1 cut(s) 91
HpaII CCGG 4 cut(s) 87, 102, 316, 339
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 1 cut(s) 116
Hpy188I TCNGA 3 cut(s) 83, 126, 294
Hpy188III TCNNGA 3 cut(s) 56, 316, 428
Hpy8I GTNNAC 1 cut(s) 116
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 3 cut(s) 56, 278, 455
HpyCH4V TGCA 3 cut(s) 109, 262, 325
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 83
HpyF3I CTNAG 1 cut(s) 390
Hsp92II CATG 5 cut(s) 109, 253, 262, 290, 437
Kpn2I TCCGGA 1 cut(s) 315
KpnI GGTACC 1 cut(s) 159
Kzo9I GATC 3 cut(s) 207, 312, 430
LmnI GCTCC 4 cut(s) 126, 146, 355, 404
Lsp1109I GCAGC 3 cut(s) 121, 151, 337
MaeIII GTNAC 2 cut(s) 200, 329
MalI GATC 3 cut(s) 209, 314, 432
MboI GATC 3 cut(s) 207, 312, 430
MboII GAAGA 2 cut(s) 128, 141
MflI RGATCY 2 cut(s) 207, 312
MhlI GDGCHC 2 cut(s) 131, 401
MluCI AATT 2 cut(s) 228, 422
MmeI TCCRAC 1 cut(s) 104
Mph1103I ATGCAT 1 cut(s) 264
MroI TCCGGA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 4 cut(s) 87, 102, 316, 339
MspR9I CCNGG 6 cut(s) 88, 102, 212, 339, 340, 354
Mva1269I GAATGC 2 cut(s) 262, 466
MvaI CCWGG 2 cut(s) 212, 354
MwoI GCNNNNNNNGC 2 cut(s) 39, 83
NciI CCSGG 4 cut(s) 88, 102, 339, 340
NdeII GATC 3 cut(s) 207, 312, 430
NlaIII CATG 5 cut(s) 109, 253, 262, 290, 437
NlaIV GGNNCC 3 cut(s) 128, 157, 358
NmuCI GTSAC 2 cut(s) 200, 329
NsiI ATGCAT 1 cut(s) 264
NspI RCATGY 2 cut(s) 109, 262
PaeI GCATGC 1 cut(s) 109
PctI GAATGC 2 cut(s) 262, 466
PfeI GAWTC 1 cut(s) 91
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 5 cut(s) 44, 76, 111, 166, 327
Psp124BI GAGCTC 1 cut(s) 401
Psp6I CCWGG 2 cut(s) 210, 352
PspGI CCWGG 2 cut(s) 210, 352
PspN4I GGNNCC 3 cut(s) 128, 157, 358
PspPI GGNCC 1 cut(s) 356
PstNI CAGNNNCTG 1 cut(s) 211
PsuI RGATCY 2 cut(s) 207, 312
RsaI GTAC 2 cut(s) 157, 280
RsaNI GTAC 2 cut(s) 156, 279
RseI CAYNNNNRTG 1 cut(s) 407
SacI GAGCTC 1 cut(s) 401
SatI GCNGC 5 cut(s) 43, 75, 110, 165, 326
Sau3AI GATC 3 cut(s) 207, 312, 430
Sau96I GGNCC 1 cut(s) 356
ScrFI CCNGG 6 cut(s) 88, 102, 212, 339, 340, 354
SduI GDGCHC 2 cut(s) 131, 401
SetI ASST 3 cut(s) 161, 401, 447
SinI GGWCC 1 cut(s) 356
SmaI CCCGGG 1 cut(s) 340
SmiMI CAYNNNNRTG 1 cut(s) 407
SphI GCATGC 1 cut(s) 109
Sse9I AATT 2 cut(s) 228, 422
SsiI CCGC 3 cut(s) 33, 43, 75
SstI GAGCTC 1 cut(s) 401
StyD4I CCNGG 6 cut(s) 86, 100, 210, 337, 338, 352
TaqI TCGA 3 cut(s) 25, 407, 459
TasI AATT 2 cut(s) 228, 422
TauI GCSGC 2 cut(s) 45, 77
TfiI GAWTC 1 cut(s) 91
TseFI GTSAC 2 cut(s) 200, 329
TseI GCWGC 3 cut(s) 109, 164, 325
Tsp45I GTSAC 2 cut(s) 200, 329
TspGWI ACGGA 2 cut(s) 188, 260
TspMI CCCGGG 1 cut(s) 338
Van91I CCANNNNNTGG 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 356
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 2 cut(s) 228, 422
XceI RCATGY 2 cut(s) 109, 262
XmaI CCCGGG 1 cut(s) 338
Zsp2I ATGCAT 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.