Rmu_ssc0000259.1_g000046

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000259.1
OG0006910
Physical Location & Seq
Reverse (-)
208930 .. 209421
492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000259.1_g000046.1.cds

Sequence Viewer

Length: 492 bp
atggttcgcacaaagcaaacaactcgacttggcggacatcgccgcctgcctcctccagaggaaggccgtcaggccgctgtccgagccgggattctccatcccggcatgcagcgtgaacgaagtcggagccctcctcttcgctcctcctcttctggtacctctgctgctactcctcctcctgtctctttggatagtctccgtgaccagatcctggtggctgactggagaatttcctgcctcactacggacatggatgacatgcattcccttctggttcgtactcgtcatgctctgaccaccctcacacaggagatccggaacttgcagcgtcaccctcccgggtttgacgctccaggtccctccaccgcagttccaacagccattgcccctgcggagagctccacttcaatggacagtgaggaatttcaagaccatgtcaccaagcttatggaggaagtcgaagggcattcccattcaaagggggagaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

17.87

Weight (kDa)

7.11

Isoelectric Point (pI)

76.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 155
AccB1I GGYRCC 1 cut(s) 155
AccB7I CCANNNNNTGG 1 cut(s) 211
AccIII TCCGGA 1 cut(s) 315
AciI CCGC 5 cut(s) 33, 43, 75, 366, 392
AclWI GGATC 2 cut(s) 202, 307
AcsI RAATTY 2 cut(s) 228, 422
AfaI GTAC 2 cut(s) 157, 280
AfiI CCNNNNNNNGG 5 cut(s) 62, 211, 244, 307, 478
AgsI TTSAA 3 cut(s) 408, 428, 477
AjnI CCWGG 2 cut(s) 210, 352
AluBI AGCT 2 cut(s) 399, 445
AluI AGCT 2 cut(s) 399, 445
Alw21I GWGCWC 1 cut(s) 401
Alw26I GTCTC 2 cut(s) 187, 200
AlwI GGATC 2 cut(s) 202, 307
AlwNI CAGNNNCTG 1 cut(s) 211
Ama87I CYCGRG 1 cut(s) 338
Aor13HI TCCGGA 1 cut(s) 315
AoxI GGCC 2 cut(s) 64, 72
ApeKI GCWGC 3 cut(s) 109, 164, 325
ApoI RAATTY 2 cut(s) 228, 422
Asp718I GGTACC 1 cut(s) 155
AspS9I GGNCC 1 cut(s) 356
AsuC2I CCSGG 4 cut(s) 88, 102, 339, 340
AsuHPI GGTGA 2 cut(s) 323, 430
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 1 cut(s) 356
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 2 cut(s) 131, 401
Bbv12I GWGCWC 1 cut(s) 401
BbvI GCAGC 3 cut(s) 121, 151, 337
BccI CCATC 1 cut(s) 105
BceAI ACGGC 1 cut(s) 51
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 2 cut(s) 212, 354
BcnI CCSGG 4 cut(s) 88, 102, 339, 340
BcoDI GTCTC 2 cut(s) 187, 200
BisI GCNGC 5 cut(s) 43, 75, 110, 165, 326
BlsI GCNGC 5 cut(s) 44, 76, 111, 166, 327
Bme1390I CCNGG 6 cut(s) 88, 102, 212, 339, 340, 354
Bme18I GGWCC 1 cut(s) 356
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 1 cut(s) 356
BmiI GGNNCC 3 cut(s) 128, 157, 358
BmrFI CCNGG 6 cut(s) 88, 102, 212, 339, 340, 354
BplI GAGNNNNNCTC 2 cut(s) 118, 150
BpmI CTGGAG 3 cut(s) 39, 244, 336
BpuMI CCSGG 4 cut(s) 88, 102, 339, 340
BsaJI CCNNGG 1 cut(s) 338
BsaWI WCCGGW 1 cut(s) 315
BsaXI ACNNNNNCTCC 2 cut(s) 160, 190
Bsc4I CCNNNNNNNGG 5 cut(s) 62, 211, 244, 307, 478
Bse1I ACTGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 381
BseAI TCCGGA 1 cut(s) 315
BseBI CCWGG 2 cut(s) 212, 354
BseDI CCNNGG 1 cut(s) 338
BseGI GGATG 2 cut(s) 97, 259
BseLI CCNNNNNNNGG 5 cut(s) 62, 211, 244, 307, 478
BseMI GCAATG 1 cut(s) 381
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 6 cut(s) 42, 123, 133, 136, 162, 165
BseXI GCAGC 3 cut(s) 121, 151, 337
BshFI GGCC 2 cut(s) 66, 74
BshNI GGYRCC 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 401
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 4 cut(s) 87, 102, 316, 339
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 5 cut(s) 62, 211, 244, 307, 478
BsmAI GTCTC 2 cut(s) 187, 200
BsmFI GGGAC 1 cut(s) 342
BsmI GAATGC 2 cut(s) 262, 466
BsnI GGCC 2 cut(s) 66, 74
BsoBI CYCGRG 1 cut(s) 338
Bsp1286I GDGCHC 2 cut(s) 131, 401
Bsp13I TCCGGA 1 cut(s) 315
Bsp143I GATC 2 cut(s) 207, 312
BspACI CCGC 5 cut(s) 33, 43, 75, 366, 392
BspANI GGCC 2 cut(s) 66, 74
BspEI TCCGGA 1 cut(s) 315
BspLI GGNNCC 3 cut(s) 128, 157, 358
BspPI GGATC 2 cut(s) 202, 307
BspT107I GGYRCC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 381
BsrI ACTGG 1 cut(s) 227
BssECI CCNNGG 1 cut(s) 338
BssMI GATC 2 cut(s) 207, 312
Bst2UI CCWGG 2 cut(s) 212, 354
Bst4CI ACNGT 1 cut(s) 416
Bst6I CTCTTC 2 cut(s) 141, 154
BstC8I GCNNGC 2 cut(s) 47, 107
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 2 cut(s) 97, 259
BstKTI GATC 2 cut(s) 210, 315
BstMAI GTCTC 2 cut(s) 187, 200
BstMBI GATC 2 cut(s) 207, 312
BstMWI GCNNNNNNNGC 2 cut(s) 39, 83
BstNI CCWGG 2 cut(s) 212, 354
BstNSI RCATGY 2 cut(s) 109, 262
BstSCI CCNGG 6 cut(s) 86, 100, 210, 337, 338, 352
BstV1I GCAGC 3 cut(s) 121, 151, 337
BstX2I RGATCY 2 cut(s) 207, 312
BstXI CCANNNNNNTGG 2 cut(s) 409, 448
BstYI RGATCY 2 cut(s) 207, 312
BsuRI GGCC 2 cut(s) 66, 74
BtgZI GCGATG 1 cut(s) 23
BtsCI GGATG 2 cut(s) 97, 259
BtsIMutI CAGTG 1 cut(s) 421
Cac8I GCNNGC 2 cut(s) 47, 107
CaiI CAGNNNCTG 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 356
Cfr9I CCCGGG 1 cut(s) 338
CseI GACGC 2 cut(s) 317, 356
Csp6I GTAC 2 cut(s) 156, 279
CviAII CATG 5 cut(s) 106, 250, 259, 287, 434
CviJI RGCY 8 cut(s) 66, 74, 86, 129, 218, 380, 399, 445
CviKI_1 RGCY 8 cut(s) 66, 74, 86, 129, 218, 380, 399, 445
CviQI GTAC 2 cut(s) 156, 279
DpnI GATC 2 cut(s) 209, 314
DpnII GATC 2 cut(s) 207, 312
Eam1104I CTCTTC 2 cut(s) 141, 154
EarI CTCTTC 2 cut(s) 141, 154
EciI GGCGGA 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 399
Eco24I GRGCYC 2 cut(s) 131, 401
Eco47I GGWCC 1 cut(s) 356
Eco53kI GAGCTC 1 cut(s) 399
Eco88I CYCGRG 1 cut(s) 338
EcoICRI GAGCTC 1 cut(s) 399
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO109I RGGNCCY 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 210, 352
EcoT22I ATGCAT 1 cut(s) 264
EcoT38I GRGCYC 2 cut(s) 131, 401
FaeI CATG 5 cut(s) 109, 253, 262, 290, 437
FaiI YATR 6 cut(s) 107, 251, 260, 288, 435, 449
FaqI GGGAC 1 cut(s) 342
FatI CATG 5 cut(s) 105, 249, 258, 286, 433
Fnu4HI GCNGC 5 cut(s) 43, 75, 110, 165, 326
FokI GGATG 2 cut(s) 84, 266
FriOI GRGCYC 2 cut(s) 131, 401
Fsp4HI GCNGC 5 cut(s) 43, 75, 110, 165, 326
GluI GCNGC 5 cut(s) 43, 75, 110, 165, 326
GsuI CTGGAG 3 cut(s) 39, 244, 336
HaeIII GGCC 2 cut(s) 66, 74
HapII CCGG 4 cut(s) 87, 102, 316, 339
HgaI GACGC 2 cut(s) 317, 356
Hin1II CATG 5 cut(s) 109, 253, 262, 290, 437
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 1 cut(s) 91
HpaII CCGG 4 cut(s) 87, 102, 316, 339
HphI GGTGA 2 cut(s) 323, 430
Hpy166II GTNNAC 1 cut(s) 116
Hpy188I TCNGA 3 cut(s) 83, 126, 294
Hpy188III TCNNGA 3 cut(s) 56, 316, 428
Hpy8I GTNNAC 1 cut(s) 116
HpyAV CCTTC 3 cut(s) 56, 278, 455
HpyCH4III ACNGT 1 cut(s) 416
HpyCH4V TGCA 3 cut(s) 109, 262, 325
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 83
Hsp92II CATG 5 cut(s) 109, 253, 262, 290, 437
Kpn2I TCCGGA 1 cut(s) 315
KpnI GGTACC 1 cut(s) 159
Kzo9I GATC 2 cut(s) 207, 312
LmnI GCTCC 4 cut(s) 126, 146, 355, 404
Lsp1109I GCAGC 3 cut(s) 121, 151, 337
MaeIII GTNAC 3 cut(s) 200, 329, 436
MalI GATC 2 cut(s) 209, 314
MboI GATC 2 cut(s) 207, 312
MboII GAAGA 2 cut(s) 128, 141
MflI RGATCY 2 cut(s) 207, 312
MhlI GDGCHC 2 cut(s) 131, 401
MluCI AATT 2 cut(s) 228, 422
MmeI TCCRAC 2 cut(s) 104, 398
Mph1103I ATGCAT 1 cut(s) 264
MroI TCCGGA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 4 cut(s) 87, 102, 316, 339
MspR9I CCNGG 6 cut(s) 88, 102, 212, 339, 340, 354
Mva1269I GAATGC 2 cut(s) 262, 466
MvaI CCWGG 2 cut(s) 212, 354
MwoI GCNNNNNNNGC 2 cut(s) 39, 83
NciI CCSGG 4 cut(s) 88, 102, 339, 340
NdeII GATC 2 cut(s) 207, 312
NlaIII CATG 5 cut(s) 109, 253, 262, 290, 437
NlaIV GGNNCC 3 cut(s) 128, 157, 358
NmuCI GTSAC 3 cut(s) 200, 329, 436
NsiI ATGCAT 1 cut(s) 264
NspI RCATGY 2 cut(s) 109, 262
PaeI GCATGC 1 cut(s) 109
PctI GAATGC 2 cut(s) 262, 466
PfeI GAWTC 1 cut(s) 91
PflFI GACNNNGTC 1 cut(s) 434
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 5 cut(s) 44, 76, 111, 166, 327
PpuMI RGGWCCY 1 cut(s) 356
Psp124BI GAGCTC 1 cut(s) 401
Psp5II RGGWCCY 1 cut(s) 356
Psp6I CCWGG 2 cut(s) 210, 352
PspGI CCWGG 2 cut(s) 210, 352
PspN4I GGNNCC 3 cut(s) 128, 157, 358
PspPI GGNCC 1 cut(s) 356
PspPPI RGGWCCY 1 cut(s) 356
PstNI CAGNNNCTG 1 cut(s) 211
PsuI RGATCY 2 cut(s) 207, 312
PsyI GACNNNGTC 1 cut(s) 434
RsaI GTAC 2 cut(s) 157, 280
RsaNI GTAC 2 cut(s) 156, 279
RseI CAYNNNNRTG 1 cut(s) 407
SacI GAGCTC 1 cut(s) 401
SatI GCNGC 5 cut(s) 43, 75, 110, 165, 326
Sau3AI GATC 2 cut(s) 207, 312
Sau96I GGNCC 1 cut(s) 356
ScrFI CCNGG 6 cut(s) 88, 102, 212, 339, 340, 354
SduI GDGCHC 2 cut(s) 131, 401
SetI ASST 4 cut(s) 161, 358, 401, 447
SinI GGWCC 1 cut(s) 356
SmaI CCCGGG 1 cut(s) 340
SmiMI CAYNNNNRTG 1 cut(s) 407
SphI GCATGC 1 cut(s) 109
Sse9I AATT 2 cut(s) 228, 422
SsiI CCGC 5 cut(s) 33, 43, 75, 366, 392
SstI GAGCTC 1 cut(s) 401
StyD4I CCNGG 6 cut(s) 86, 100, 210, 337, 338, 352
TaaI ACNGT 1 cut(s) 416
TaqI TCGA 2 cut(s) 25, 459
TasI AATT 2 cut(s) 228, 422
TauI GCSGC 2 cut(s) 45, 77
TfiI GAWTC 1 cut(s) 91
TscAI CASTG 1 cut(s) 421
TseFI GTSAC 3 cut(s) 200, 329, 436
TseI GCWGC 3 cut(s) 109, 164, 325
Tsp45I GTSAC 3 cut(s) 200, 329, 436
TspGWI ACGGA 2 cut(s) 188, 260
TspMI CCCGGG 1 cut(s) 338
TspRI CASTG 1 cut(s) 421
Tth111I GACNNNGTC 1 cut(s) 434
Van91I CCANNNNNTGG 1 cut(s) 211
VpaK11BI GGWCC 1 cut(s) 356
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 2 cut(s) 228, 422
XceI RCATGY 2 cut(s) 109, 262
XmaI CCCGGG 1 cut(s) 338
Zsp2I ATGCAT 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.