Rmu_sc0027068.1_g000002

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0027068.1
OG0006910
Physical Location & Seq
Reverse (-)
4511 .. 4792
282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0027068.1_g000002.1.cds

Sequence Viewer

Length: 282 bp
atggtggctgactggcgcattgcttgcctcacgacggacatggacgacatgcattctcttctggttcggactcgtcatgccctgaccactctcacacaggagatccggaacttgcagcgccaccctcccgggtttgacgctcccggtccttcccatgccatgcaggagcctgtcgcaccagaggaaagctcggattccatggatgaagaggaatttctggacaccgtcaccaagctttgtgaggaatttgacgtgccctcccattcaaagggggagaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

93

Amino Acids

10.51

Weight (kDa)

4.6

Isoelectric Point (pI)

71.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 105
AclWI GGATC 1 cut(s) 97
AcsI RAATTY 2 cut(s) 212, 245
AfiI CCNNNNNNNGG 2 cut(s) 34, 268
AgsI TTSAA 1 cut(s) 267
AjiI CACGTC 1 cut(s) 253
AluBI AGCT 2 cut(s) 189, 235
AluI AGCT 2 cut(s) 189, 235
AlwI GGATC 1 cut(s) 97
Ama87I CYCGRG 1 cut(s) 128
Aor13HI TCCGGA 1 cut(s) 105
ApeKI GCWGC 1 cut(s) 115
ApoI RAATTY 2 cut(s) 212, 245
AspLEI GCGC 2 cut(s) 18, 120
AspS9I GGNCC 1 cut(s) 146
AsuC2I CCSGG 3 cut(s) 129, 130, 144
AsuHPI GGTGA 1 cut(s) 220
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 1 cut(s) 146
BaeGI GKGCMC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 127
BcnI CCSGG 3 cut(s) 129, 130, 144
BfoI RGCGCY 1 cut(s) 121
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
Bme1390I CCNGG 3 cut(s) 129, 130, 144
Bme18I GGWCC 1 cut(s) 146
BmeT110I CYCGRG 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 3 cut(s) 129, 130, 144
BplI GAGNNNNNCTC 2 cut(s) 173, 205
BpuMI CCSGG 3 cut(s) 129, 130, 144
BsaJI CCNNGG 2 cut(s) 128, 198
BsaWI WCCGGW 1 cut(s) 105
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 268
Bse1I ACTGG 1 cut(s) 17
Bse3DI GCAATG 1 cut(s) 18
BseAI TCCGGA 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 128, 198
BseGI GGATG 1 cut(s) 208
BseLI CCNNNNNNNGG 2 cut(s) 34, 268
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 1 cut(s) 17
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 1 cut(s) 127
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 3 cut(s) 106, 129, 144
BslI CCNNNNNNNGG 2 cut(s) 34, 268
BsmI GAATGC 1 cut(s) 52
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 258
Bsp13I TCCGGA 1 cut(s) 105
Bsp143I GATC 1 cut(s) 102
Bsp19I CCATGG 1 cut(s) 198
BspEI TCCGGA 1 cut(s) 105
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 1 cut(s) 97
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 1 cut(s) 17
BssECI CCNNGG 2 cut(s) 128, 198
BssMI GATC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 226
Bst6I CTCTTC 2 cut(s) 63, 201
BstAPI GCANNNNNTGC 1 cut(s) 24
BstC8I GCNNGC 1 cut(s) 25
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 1 cut(s) 208
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 2 cut(s) 18, 120
BstKTI GATC 1 cut(s) 105
BstMBI GATC 1 cut(s) 102
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 3 cut(s) 127, 128, 142
BstSLI GKGCMC 1 cut(s) 258
BstV1I GCAGC 1 cut(s) 127
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BtgI CCRYGG 1 cut(s) 198
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 1 cut(s) 208
Cac8I GCNNGC 1 cut(s) 25
CfoI GCGC 2 cut(s) 18, 120
Cfr13I GGNCC 1 cut(s) 146
Cfr9I CCCGGG 1 cut(s) 128
CseI GACGC 1 cut(s) 146
CviAII CATG 6 cut(s) 40, 49, 77, 155, 160, 199
CviJI RGCY 4 cut(s) 8, 169, 189, 235
CviKI_1 RGCY 4 cut(s) 8, 169, 189, 235
DpnI GATC 1 cut(s) 104
DpnII GATC 1 cut(s) 102
Eam1104I CTCTTC 2 cut(s) 63, 201
EarI CTCTTC 2 cut(s) 63, 201
Eco130I CCWWGG 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 146
Eco88I CYCGRG 1 cut(s) 128
EcoT14I CCWWGG 1 cut(s) 198
EcoT22I ATGCAT 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 6 cut(s) 43, 52, 80, 158, 163, 202
FaiI YATR 6 cut(s) 41, 50, 78, 156, 161, 200
FatI CATG 6 cut(s) 39, 48, 76, 154, 159, 198
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 116
GlaI GCGC 2 cut(s) 17, 119
GluI GCNGC 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 121
HapII CCGG 3 cut(s) 106, 129, 144
HgaI GACGC 1 cut(s) 146
HhaI GCGC 2 cut(s) 18, 120
Hin1II CATG 6 cut(s) 43, 52, 80, 158, 163, 202
Hin6I GCGC 2 cut(s) 16, 118
HinP1I GCGC 2 cut(s) 16, 118
HindIII AAGCTT 1 cut(s) 233
HinfI GANTC 2 cut(s) 70, 194
HpaII CCGG 3 cut(s) 106, 129, 144
HphI GGTGA 1 cut(s) 220
Hpy188I TCNGA 2 cut(s) 69, 193
Hpy188III TCNNGA 3 cut(s) 31, 106, 218
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 1 cut(s) 159
HpyCH4III ACNGT 1 cut(s) 226
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 3 cut(s) 52, 115, 163
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpySE526I ACGT 1 cut(s) 252
Hsp92II CATG 6 cut(s) 43, 52, 80, 158, 163, 202
HspAI GCGC 2 cut(s) 16, 118
Kpn2I TCCGGA 1 cut(s) 105
Kzo9I GATC 1 cut(s) 102
LmnI GCTCC 2 cut(s) 145, 166
Lsp1109I GCAGC 1 cut(s) 127
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 226
MalI GATC 1 cut(s) 104
MboI GATC 1 cut(s) 102
MboII GAAGA 2 cut(s) 50, 218
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 2 cut(s) 212, 245
MlyI GAGTC 1 cut(s) 64
MnlI CCTC 6 cut(s) 38, 135, 175, 202, 235, 268
Mph1103I ATGCAT 1 cut(s) 54
MroI TCCGGA 1 cut(s) 105
MspI CCGG 3 cut(s) 106, 129, 144
MspR9I CCNGG 3 cut(s) 129, 130, 144
Mva1269I GAATGC 1 cut(s) 52
MwoI GCNNNNNNNGC 1 cut(s) 24
NciI CCSGG 3 cut(s) 129, 130, 144
NcoI CCATGG 1 cut(s) 198
NdeII GATC 1 cut(s) 102
NlaIII CATG 6 cut(s) 43, 52, 80, 158, 163, 202
NlaIV GGNNCC 1 cut(s) 168
NmuCI GTSAC 1 cut(s) 226
NsiI ATGCAT 1 cut(s) 54
NspI RCATGY 1 cut(s) 52
PctI GAATGC 1 cut(s) 52
PfeI GAWTC 1 cut(s) 194
PflFI GACNNNGTC 1 cut(s) 224
PkrI GCNGC 1 cut(s) 117
PleI GAGTC 1 cut(s) 64
PpsI GAGTC 1 cut(s) 64
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 1 cut(s) 146
PsuI RGATCY 1 cut(s) 102
PsyI GACNNNGTC 1 cut(s) 224
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 1 cut(s) 102
Sau96I GGNCC 1 cut(s) 146
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 3 cut(s) 129, 130, 144
SduI GDGCHC 1 cut(s) 258
SetI ASST 3 cut(s) 191, 237, 255
SinI GGWCC 1 cut(s) 146
SmaI CCCGGG 1 cut(s) 130
Sse9I AATT 2 cut(s) 212, 245
StyD4I CCNGG 3 cut(s) 127, 128, 142
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 1 cut(s) 226
TaiI ACGT 1 cut(s) 255
TasI AATT 2 cut(s) 212, 245
TfiI GAWTC 1 cut(s) 194
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 219
TspGWI ACGGA 1 cut(s) 50
TspMI CCCGGG 1 cut(s) 128
Tth111I GACNNNGTC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 146
XapI RAATTY 2 cut(s) 212, 245
XceI RCATGY 1 cut(s) 52
XmaI CCCGGG 1 cut(s) 128
Zsp2I ATGCAT 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.