Rmu_sc0030281.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0030281.1
OG0006910
Physical Location & Seq
Reverse (-)
897 .. 1133
237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0030281.1_g000001.1.cds

Sequence Viewer

Length: 237 bp
atgcattcccttctggttcgtactcgtcatgctctgaccaccctcacacaggagatccgaaacttgcagcgtcaccctcccgggtttgacgctcctggtccctccactgctgttccagtagccatcgctcctgaggagagctccacttcgatggacagcgaggaatttcaggatcaagtcgtcaagcttatggaggaagtcgaaggacattcccattcaaagggggagaagaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

78

Amino Acids

8.63

Weight (kDa)

5.64

Isoelectric Point (pI)

70.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 49, 180
AcsI RAATTY 1 cut(s) 164
AfaI GTAC 1 cut(s) 22
AfiI CCNNNNNNNGG 2 cut(s) 49, 220
AgsI TTSAA 1 cut(s) 219
AjnI CCWGG 1 cut(s) 94
AluBI AGCT 2 cut(s) 141, 187
AluI AGCT 2 cut(s) 141, 187
Alw21I GWGCWC 1 cut(s) 143
AlwI GGATC 2 cut(s) 49, 180
Ama87I CYCGRG 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 67
ApoI RAATTY 1 cut(s) 164
AspS9I GGNCC 1 cut(s) 98
AsuC2I CCSGG 2 cut(s) 81, 82
AsuHPI GGTGA 1 cut(s) 65
AvaI CYCGRG 1 cut(s) 80
AvaII GGWCC 1 cut(s) 98
AxyI CCTNAGG 1 cut(s) 132
BanII GRGCYC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 1 cut(s) 79
BccI CCATC 2 cut(s) 131, 145
BciT130I CCWGG 1 cut(s) 96
BcnI CCSGG 2 cut(s) 81, 82
BisI GCNGC 1 cut(s) 68
BlsI GCNGC 1 cut(s) 69
Bme1390I CCNGG 3 cut(s) 81, 82, 96
Bme18I GGWCC 1 cut(s) 98
BmeT110I CYCGRG 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 1 cut(s) 100
BmrFI CCNGG 3 cut(s) 81, 82, 96
BplI GAGNNNNNCTC 2 cut(s) 125, 157
BpuMI CCSGG 2 cut(s) 81, 82
BsaJI CCNNGG 1 cut(s) 80
BsaXI ACNNNNNCTCC 1 cut(s) 218
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 220
Bse1I ACTGG 1 cut(s) 116
Bse21I CCTNAGG 1 cut(s) 132
BseBI CCWGG 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 80
BseLI CCNNNNNNNGG 2 cut(s) 49, 220
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 1 cut(s) 116
BseRI GAGGAG 1 cut(s) 149
BseXI GCAGC 1 cut(s) 79
BsiHKAI GWGCWC 1 cut(s) 143
BsiHKCI CYCGRG 1 cut(s) 80
BsiSI CCGG 1 cut(s) 81
BslFI GGGAC 1 cut(s) 84
BslI CCNNNNNNNGG 2 cut(s) 49, 220
BsmFI GGGAC 1 cut(s) 84
BsmI GAATGC 1 cut(s) 4
BsoBI CYCGRG 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 2 cut(s) 54, 172
BspCNI CTCAG 1 cut(s) 124
BspLI GGNNCC 1 cut(s) 100
BspPI GGATC 2 cut(s) 49, 180
BsrI ACTGG 1 cut(s) 116
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 2 cut(s) 54, 172
Bst2UI CCWGG 1 cut(s) 96
BstDEI CTNAG 1 cut(s) 132
BstENI CCTNNNNNAGG 1 cut(s) 47
BstKTI GATC 2 cut(s) 57, 175
BstMBI GATC 2 cut(s) 54, 172
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 3 cut(s) 79, 80, 94
BstV1I GCAGC 1 cut(s) 79
BstX2I RGATCY 1 cut(s) 54
BstXI CCANNNNNNTGG 1 cut(s) 151
BstYI RGATCY 1 cut(s) 54
Bsu36I CCTNAGG 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 109
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 105
Cfr13I GGNCC 1 cut(s) 98
Cfr9I CCCGGG 1 cut(s) 80
CseI GACGC 2 cut(s) 59, 98
Csp6I GTAC 1 cut(s) 21
CviAII CATG 1 cut(s) 29
CviJI RGCY 3 cut(s) 122, 141, 187
CviKI_1 RGCY 3 cut(s) 122, 141, 187
CviQI GTAC 1 cut(s) 21
DdeI CTNAG 1 cut(s) 132
DpnI GATC 2 cut(s) 56, 174
DpnII GATC 2 cut(s) 54, 172
Ecl136II GAGCTC 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 141
Eco81I CCTNAGG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 141
EcoNI CCTNNNNNAGG 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 94
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 143
FaeI CATG 1 cut(s) 32
FaiI YATR 2 cut(s) 30, 191
FaqI GGGAC 1 cut(s) 84
FatI CATG 1 cut(s) 28
Fnu4HI GCNGC 1 cut(s) 68
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 1 cut(s) 68
GluI GCNGC 1 cut(s) 68
HapII CCGG 1 cut(s) 81
HgaI GACGC 2 cut(s) 59, 98
Hin1II CATG 1 cut(s) 32
HindIII AAGCTT 1 cut(s) 185
HpaII CCGG 1 cut(s) 81
HphI GGTGA 1 cut(s) 65
Hpy188I TCNGA 2 cut(s) 36, 59
Hpy188III TCNNGA 2 cut(s) 131, 170
HpyAV CCTTC 2 cut(s) 20, 197
HpyCH4V TGCA 2 cut(s) 4, 67
HpyF3I CTNAG 1 cut(s) 132
Hsp92II CATG 1 cut(s) 32
Kzo9I GATC 2 cut(s) 54, 172
LmnI GCTCC 3 cut(s) 97, 133, 146
LpnPI CCDG 7 cut(s) 35, 81, 94, 108, 129, 144, 155
Lsp1109I GCAGC 1 cut(s) 79
MaeIII GTNAC 1 cut(s) 71
MalI GATC 2 cut(s) 56, 174
MboI GATC 2 cut(s) 54, 172
MflI RGATCY 1 cut(s) 54
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 1 cut(s) 164
MnlI CCTC 6 cut(s) 53, 87, 112, 127, 154, 187
Mph1103I ATGCAT 1 cut(s) 6
MslI CAYNNNNRTG 1 cut(s) 149
MspI CCGG 1 cut(s) 81
MspR9I CCNGG 3 cut(s) 81, 82, 96
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 1 cut(s) 96
NciI CCSGG 2 cut(s) 81, 82
NdeII GATC 2 cut(s) 54, 172
NlaIII CATG 1 cut(s) 32
NlaIV GGNNCC 1 cut(s) 100
NmuCI GTSAC 1 cut(s) 71
NsiI ATGCAT 1 cut(s) 6
PctI GAATGC 1 cut(s) 4
PkrI GCNGC 1 cut(s) 69
Psp124BI GAGCTC 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 100
PspPI GGNCC 1 cut(s) 98
PsuI RGATCY 1 cut(s) 54
RsaI GTAC 1 cut(s) 22
RsaNI GTAC 1 cut(s) 21
RseI CAYNNNNRTG 1 cut(s) 149
SacI GAGCTC 1 cut(s) 143
SatI GCNGC 1 cut(s) 68
Sau3AI GATC 2 cut(s) 54, 172
Sau96I GGNCC 1 cut(s) 98
ScrFI CCNGG 3 cut(s) 81, 82, 96
SduI GDGCHC 1 cut(s) 143
SetI ASST 2 cut(s) 143, 189
SinI GGWCC 1 cut(s) 98
SmaI CCCGGG 1 cut(s) 82
SmiMI CAYNNNNRTG 1 cut(s) 149
Sse9I AATT 1 cut(s) 164
SstI GAGCTC 1 cut(s) 143
StyD4I CCNGG 3 cut(s) 79, 80, 94
TaqI TCGA 2 cut(s) 149, 201
TasI AATT 1 cut(s) 164
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 1 cut(s) 67
Tsp45I GTSAC 1 cut(s) 71
TspMI CCCGGG 1 cut(s) 80
TspRI CASTG 1 cut(s) 112
VpaK11BI GGWCC 1 cut(s) 98
XagI CCTNNNNNAGG 1 cut(s) 47
XapI RAATTY 1 cut(s) 164
XmaI CCCGGG 1 cut(s) 80
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.