Rmu_sc0006069.1_g000007

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006069.1
OG0006364
Physical Location & Seq
Forward (+)
34161 .. 35748
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 660 bp
atggcttcgtcgtcctcggatcctggtccgaagaccgaaagcggaagcggcagcggcggcggagcggaggcctcggaagcggcggcggcggctgcggcggcggtggcgaccgatcagcaattgctttacagagggctgaagaaagcgaagaaagagagaggttgcacggccaaagagcgcatcagcaaaatgcctccctgtgccgctggaaaacgcagctccatttaccgtggagtcactcggcataggtggacgggtcgttacgaagctcatctctgggataaaagtacctggaaccagaaccagaataagaagggaaagcaagtttacttgggcgcgtatgatgatgaagaggctgcagccagagcttatgatcttgctgccttgaaatattggggccctggcactctgattaatttccctgttactgactacacaagagatctcgaagagatgcagaatgtgtcaagggaggaatatcttgcatctcttcgcaggtgtggatatctaagaacatatttgaatctgatagctgtcacgttgcttatactagatttttctctggcaggaagagcagtggcttctctagaggaatgtctaaatatcgcgcgctctcaaggtatggtttacatttatacgttacttgtatgcatcatataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

219

Amino Acids

24.03

Weight (kDa)

9.02

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 76 - 133 9e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 486
Acc36I ACCTGC 1 cut(s) 486
AccBSI CCGCTC 1 cut(s) 65
AccII CGCG 3 cut(s) 338, 608, 610
AclWI GGATC 2 cut(s) 14, 27
AcoI YGGCCR 1 cut(s) 168
AcuI CTGAAG 1 cut(s) 158
AfaI GTAC 1 cut(s) 289
AgsI TTSAA 2 cut(s) 388, 523
AjnI CCWGG 3 cut(s) 22, 290, 400
AluBI AGCT 4 cut(s) 219, 269, 368, 533
AluI AGCT 4 cut(s) 219, 269, 368, 533
AlwI GGATC 2 cut(s) 14, 27
AoxI GGCC 3 cut(s) 69, 168, 397
ApaI GGGCCC 1 cut(s) 401
ApeKI GCWGC 6 cut(s) 51, 92, 216, 356, 359, 380
AseI ATTAAT 1 cut(s) 414
AspLEI GCGC 4 cut(s) 180, 338, 610, 612
AspS9I GGNCC 3 cut(s) 26, 397, 398
AvaII GGWCC 1 cut(s) 26
BaeGI GKGCMC 1 cut(s) 401
BamHI GGATCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 401
BbsI GAAGAC 1 cut(s) 38
BbvI GCAGC 6 cut(s) 63, 79, 228, 343, 367, 371
BceAI ACGGC 1 cut(s) 183
BciT130I CCWGG 3 cut(s) 24, 292, 402
BfaI CTAG 2 cut(s) 551, 587
BfmI CTRYAG 1 cut(s) 357
BfuAI ACCTGC 1 cut(s) 486
BglII AGATCT 1 cut(s) 442
Bme1390I CCNGG 3 cut(s) 24, 292, 402
Bme18I GGWCC 1 cut(s) 26
BmgT120I GGNCC 3 cut(s) 26, 397, 398
BmiI GGNNCC 4 cut(s) 21, 296, 398, 399
BmrFI CCNGG 3 cut(s) 24, 292, 402
BmsI GCATC 3 cut(s) 189, 444, 494
BpiI GAAGAC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 600
BsaJI CCNNGG 4 cut(s) 15, 72, 229, 400
BseBI CCWGG 3 cut(s) 24, 292, 402
BseDI CCNNGG 4 cut(s) 15, 72, 229, 400
BsePI GCGCGC 1 cut(s) 608
BseSI GKGCMC 1 cut(s) 401
BseXI GCAGC 6 cut(s) 63, 79, 228, 343, 367, 371
Bsh1236I CGCG 3 cut(s) 338, 608, 610
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 3 cut(s) 71, 170, 399
BsiEI CGRYCG 1 cut(s) 111
BsnI GGCC 3 cut(s) 71, 170, 399
Bsp120I GGGCCC 1 cut(s) 397
Bsp1286I GDGCHC 1 cut(s) 401
Bsp143I GATC 4 cut(s) 19, 112, 373, 442
BspANI GGCC 3 cut(s) 71, 170, 399
BspFNI CGCG 3 cut(s) 338, 608, 610
BspLI GGNNCC 4 cut(s) 21, 296, 398, 399
BspMAI CTGCAG 1 cut(s) 361
BspMI ACCTGC 1 cut(s) 486
BspPI GGATC 2 cut(s) 14, 27
BspQI GCTCTTC 1 cut(s) 565
BsrBI CCGCTC 1 cut(s) 65
BssECI CCNNGG 4 cut(s) 15, 72, 229, 400
BssHII GCGCGC 1 cut(s) 608
BssMI GATC 4 cut(s) 19, 112, 373, 442
Bst2UI CCWGG 3 cut(s) 24, 292, 402
Bst4CI ACNGT 1 cut(s) 230
Bst6I CTCTTC 4 cut(s) 345, 444, 495, 565
BstC8I GCNNGC 1 cut(s) 610
BstDEI CTNAG 1 cut(s) 509
BstDSI CCRYGG 1 cut(s) 229
BstFNI CGCG 3 cut(s) 338, 608, 610
BstHHI GCGC 4 cut(s) 180, 338, 610, 612
BstKTI GATC 4 cut(s) 22, 115, 376, 445
BstMBI GATC 4 cut(s) 19, 112, 373, 442
BstMCI CGRYCG 1 cut(s) 111
BstNI CCWGG 3 cut(s) 24, 292, 402
BstSCI CCNGG 3 cut(s) 22, 290, 400
BstSFI CTRYAG 1 cut(s) 357
BstSLI GKGCMC 1 cut(s) 401
BstUI CGCG 3 cut(s) 338, 608, 610
BstV1I GCAGC 6 cut(s) 63, 79, 228, 343, 367, 371
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 2 cut(s) 19, 442
BstYI RGATCY 2 cut(s) 19, 442
BsuRI GGCC 3 cut(s) 71, 170, 399
BtgI CCRYGG 1 cut(s) 229
BtsI GCAGTG 1 cut(s) 582
BtsIMutI CAGTG 1 cut(s) 582
BveI ACCTGC 1 cut(s) 486
Cac8I GCNNGC 1 cut(s) 610
CfoI GCGC 4 cut(s) 180, 338, 610, 612
Cfr13I GGNCC 3 cut(s) 26, 397, 398
Csp6I GTAC 1 cut(s) 288
CviQI GTAC 1 cut(s) 288
DdeI CTNAG 1 cut(s) 509
DpnI GATC 4 cut(s) 21, 114, 375, 444
DpnII GATC 4 cut(s) 19, 112, 373, 442
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 4 cut(s) 345, 444, 495, 565
EarI CTCTTC 4 cut(s) 345, 444, 495, 565
EciI GGCGGA 1 cut(s) 75
Eco147I AGGCCT 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 401
Eco32I GATATC 1 cut(s) 506
Eco47I GGWCC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 158
EcoO109I RGGNCCY 2 cut(s) 397, 398
EcoRII CCWGG 3 cut(s) 22, 290, 400
EcoRV GATATC 1 cut(s) 506
EcoT22I ATGCAT 1 cut(s) 653
EcoT38I GRGCYC 1 cut(s) 401
FriOI GRGCYC 1 cut(s) 401
FspBI CTAG 2 cut(s) 551, 587
GlaI GCGC 4 cut(s) 179, 337, 609, 611
HaeIII GGCC 3 cut(s) 71, 170, 399
HhaI GCGC 4 cut(s) 180, 338, 610, 612
Hin6I GCGC 4 cut(s) 178, 336, 608, 610
HinP1I GCGC 4 cut(s) 178, 336, 608, 610
HinfI GANTC 2 cut(s) 234, 523
Hpy166II GTNNAC 3 cut(s) 252, 328, 628
Hpy188I TCNGA 5 cut(s) 19, 30, 76, 411, 528
Hpy188III TCNNGA 2 cut(s) 446, 587
Hpy8I GTNNAC 3 cut(s) 252, 328, 628
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 1 cut(s) 307
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4IV ACGT 2 cut(s) 539, 638
HpyCH4V TGCA 5 cut(s) 165, 359, 457, 485, 651
HpyF3I CTNAG 1 cut(s) 509
HpySE526I ACGT 2 cut(s) 539, 638
HspAI GCGC 4 cut(s) 178, 336, 608, 610
Kzo9I GATC 4 cut(s) 19, 112, 373, 442
LguI GCTCTTC 1 cut(s) 565
LmnI GCTCC 2 cut(s) 62, 224
Lsp1109I GCAGC 6 cut(s) 63, 79, 228, 343, 367, 371
LweI GCATC 3 cut(s) 189, 444, 494
MaeI CTAG 2 cut(s) 551, 587
MaeII ACGT 2 cut(s) 539, 638
MaeIII GTNAC 5 cut(s) 235, 260, 424, 535, 639
MalI GATC 4 cut(s) 21, 114, 375, 444
MbiI CCGCTC 1 cut(s) 65
MboI GATC 4 cut(s) 19, 112, 373, 442
MboII GAAGA 7 cut(s) 43, 151, 160, 362, 461, 482, 582
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 2 cut(s) 19, 442
MhlI GDGCHC 1 cut(s) 401
MluCI AATT 2 cut(s) 119, 415
MlyI GAGTC 1 cut(s) 243
MnlI CCTC 9 cut(s) 25, 61, 82, 125, 152, 204, 346, 466, 583
Mph1103I ATGCAT 1 cut(s) 653
MseI TTAA 1 cut(s) 414
MspA1I CMGCKG 2 cut(s) 54, 206
MspR9I CCNGG 3 cut(s) 24, 292, 402
MunI CAATTG 1 cut(s) 119
MvaI CCWGG 3 cut(s) 24, 292, 402
MvnI CGCG 3 cut(s) 338, 608, 610
NdeII GATC 4 cut(s) 19, 112, 373, 442
NlaIV GGNNCC 4 cut(s) 21, 296, 398, 399
NmeAIII GCCGAG 1 cut(s) 220
NmuCI GTSAC 2 cut(s) 235, 535
NsiI ATGCAT 1 cut(s) 653
PaqCI CACCTGC 1 cut(s) 486
PauI GCGCGC 1 cut(s) 608
PceI AGGCCT 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 565
PfeI GAWTC 1 cut(s) 523
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PshBI ATTAAT 1 cut(s) 414
Psp6I CCWGG 3 cut(s) 22, 290, 400
PspGI CCWGG 3 cut(s) 22, 290, 400
PspN4I GGNNCC 4 cut(s) 21, 296, 398, 399
PspOMI GGGCCC 1 cut(s) 397
PspPI GGNCC 3 cut(s) 26, 397, 398
PstI CTGCAG 1 cut(s) 361
PsuI RGATCY 2 cut(s) 19, 442
PteI GCGCGC 1 cut(s) 608
RsaI GTAC 1 cut(s) 289
RsaNI GTAC 1 cut(s) 288
SapI GCTCTTC 1 cut(s) 565
SaqAI TTAA 1 cut(s) 414
Sau3AI GATC 4 cut(s) 19, 112, 373, 442
Sau96I GGNCC 3 cut(s) 26, 397, 398
SchI GAGTC 1 cut(s) 243
ScrFI CCNGG 3 cut(s) 24, 292, 402
SduI GDGCHC 1 cut(s) 401
SfaNI GCATC 3 cut(s) 189, 444, 494
SfcI CTRYAG 1 cut(s) 357
SinI GGWCC 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 615
SmoI CTYRAG 1 cut(s) 615
Sse9I AATT 2 cut(s) 119, 415
SseBI AGGCCT 1 cut(s) 71
SspI AATATT 1 cut(s) 392
SspMI CTAG 2 cut(s) 551, 587
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 3 cut(s) 22, 290, 400
TaaI ACNGT 1 cut(s) 230
TaiI ACGT 2 cut(s) 542, 641
TaqI TCGA 1 cut(s) 447
TaqII GACCGA 2 cut(s) 50, 125
TasI AATT 2 cut(s) 119, 415
TfiI GAWTC 1 cut(s) 523
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 1 cut(s) 582
TseFI GTSAC 2 cut(s) 235, 535
TseI GCWGC 6 cut(s) 51, 92, 216, 356, 359, 380
Tsp45I GTSAC 2 cut(s) 235, 535
TspDTI ATGAA 1 cut(s) 363
TspRI CASTG 1 cut(s) 582
VpaK11BI GGWCC 1 cut(s) 26
VspI ATTAAT 1 cut(s) 414
XbaI TCTAGA 1 cut(s) 586
XspI CTAG 2 cut(s) 551, 587
Zsp2I ATGCAT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.