Rmu_sc0006117.1_g000001

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006117.1
OG0002090
Physical Location & Seq
Reverse (-)
2 .. 1400
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 693 bp
ctgcctatattgagtagcaagtcaggttgtaatccacagtctagagacttgattttgcagtcagtagaaagaattttatcatctccaaaagcttggcctattctattaaatggtgctgttaggaaaggggagcacatagtgccaccatcagcccttgatctcttgatgagagtttcttttccttcaccttcagcgcgagttaaggcaactgagagatttgaagctgtctatccaaccttgaaagagattgcccttgctgggtccccaggaagcaaaacagtgaggcaggttgcacagaagacactgaattattcagttaaagctgttaaagaaggcatccccgatctctctaaggaggcaaccgctatatttatctggtgtttgacacaaagtcccgaatgttacaagcaatgggacatgctttatctggacaatcttgatgcaagtgttattgtgctgaaaaagttatctgacgagtggaaggatcactccgttaaacatgcctctcttgaccccttgagggaaaccttgaagagtttcagggagaagaatgaaaaagcattgccaaacggagatgatttggtgcgccattcgttgttgaaggatgcagataaatattgcaagctgattttaggacggtcatctcaaggccgtggatgcatgaaaagcatggttctggtgtctgttgccctt
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

231

Amino Acids

25.63

Weight (kDa)

9.32

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 40 - 220 7.1e-14 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 277
AccII CGCG 1 cut(s) 196
AciI CCGC 1 cut(s) 363
AclWI GGATC 1 cut(s) 492
AcsI RAATTY 1 cut(s) 72
AcuI CTGAAG 1 cut(s) 174
AdeI CACNNNGTG 1 cut(s) 139
AfiI CCNNNNNNNGG 2 cut(s) 258, 520
AgsI TTSAA 4 cut(s) 221, 241, 532, 601
AhdI GACNNNNNGTC 1 cut(s) 390
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 4 cut(s) 92, 224, 323, 625
AluI AGCT 4 cut(s) 92, 224, 323, 625
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 1 cut(s) 39
AlwI GGATC 1 cut(s) 492
AoxI GGCC 2 cut(s) 95, 649
ApoI RAATTY 1 cut(s) 72
Asp700I GAANNNNTTC 1 cut(s) 536
AspLEI GCGC 2 cut(s) 196, 588
AspS9I GGNCC 1 cut(s) 261
AsuHPI GGTGA 1 cut(s) 177
AvaII GGWCC 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 305
Bbv12I GWGCWC 1 cut(s) 135
BccI CCATC 1 cut(s) 154
BceAI ACGGC 1 cut(s) 636
BciT130I CCWGG 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 39
BfaI CTAG 1 cut(s) 42
BfuAI ACCTGC 1 cut(s) 277
Bme1390I CCNGG 1 cut(s) 267
Bme18I GGWCC 1 cut(s) 261
BmeRI GACNNNNNGTC 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 261
BmiI GGNNCC 2 cut(s) 262, 263
BmrFI CCNGG 1 cut(s) 267
BmsI GCATC 4 cut(s) 345, 430, 595, 647
BpiI GAAGAC 1 cut(s) 305
BpuEI CTTGAG 2 cut(s) 538, 630
BsaJI CCNNGG 2 cut(s) 265, 652
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 520
Bse3DI GCAATG 2 cut(s) 416, 560
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 265, 652
BseGI GGATG 3 cut(s) 336, 610, 662
BseLI CCNNNNNNNGG 2 cut(s) 258, 520
BseMI GCAATG 2 cut(s) 416, 560
BseMII CTCAG 1 cut(s) 201
BseYI CCCAGC 1 cut(s) 257
Bsh1236I CGCG 1 cut(s) 196
BshFI GGCC 2 cut(s) 97, 651
BsiHKAI GWGCWC 1 cut(s) 135
BslFI GGGAC 3 cut(s) 247, 378, 428
BslI CCNNNNNNNGG 2 cut(s) 258, 520
BsmAI GTCTC 1 cut(s) 39
BsmFI GGGAC 3 cut(s) 247, 378, 428
BsnI GGCC 2 cut(s) 97, 651
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 3 cut(s) 157, 343, 484
BspACI CCGC 1 cut(s) 363
BspANI GGCC 2 cut(s) 97, 651
BspCNI CTCAG 1 cut(s) 202
BspFNI CGCG 1 cut(s) 196
BspLI GGNNCC 2 cut(s) 262, 263
BspMI ACCTGC 1 cut(s) 277
BspPI GGATC 1 cut(s) 492
BsrDI GCAATG 2 cut(s) 416, 560
BssECI CCNNGG 2 cut(s) 265, 652
BssMI GATC 3 cut(s) 157, 343, 484
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 3 cut(s) 39, 280, 639
Bst6I CTCTTC 1 cut(s) 527
BstAPI GCANNNNNTGC 1 cut(s) 139
BstC8I GCNNGC 1 cut(s) 623
BstDEI CTNAG 2 cut(s) 210, 351
BstDSI CCRYGG 1 cut(s) 652
BstF5I GGATG 3 cut(s) 336, 610, 662
BstFNI CGCG 1 cut(s) 196
BstHHI GCGC 2 cut(s) 196, 588
BstKTI GATC 3 cut(s) 160, 346, 487
BstMAI GTCTC 1 cut(s) 39
BstMBI GATC 3 cut(s) 157, 343, 484
BstMWI GCNNNNNNNGC 3 cut(s) 139, 657, 666
BstNI CCWGG 1 cut(s) 267
BstNSI RCATGY 2 cut(s) 421, 503
BstSCI CCNGG 1 cut(s) 265
BstUI CGCG 1 cut(s) 196
BstV2I GAAGAC 1 cut(s) 305
BstXI CCANNNNNNTGG 1 cut(s) 93
BsuRI GGCC 2 cut(s) 97, 651
BtgI CCRYGG 1 cut(s) 652
BtsCI GGATG 3 cut(s) 336, 610, 662
BtsIMutI CAGTG 2 cut(s) 285, 302
BveI ACCTGC 1 cut(s) 277
Cac8I GCNNGC 1 cut(s) 623
CfoI GCGC 2 cut(s) 196, 588
Cfr13I GGNCC 1 cut(s) 261
CviAII CATG 4 cut(s) 418, 500, 661, 670
CviJI RGCY 7 cut(s) 92, 97, 152, 224, 323, 625, 651
CviKI_1 RGCY 7 cut(s) 92, 97, 152, 224, 323, 625, 651
DdeI CTNAG 2 cut(s) 210, 351
DpnI GATC 3 cut(s) 159, 345, 486
DpnII GATC 3 cut(s) 157, 343, 484
DraIII CACNNNGTG 1 cut(s) 139
DriI GACNNNNNGTC 1 cut(s) 390
Eam1104I CTCTTC 1 cut(s) 527
Eam1105I GACNNNNNGTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 527
Eco47I GGWCC 1 cut(s) 261
Eco57I CTGAAG 1 cut(s) 174
EcoO109I RGGNCCY 1 cut(s) 261
EcoRII CCWGG 1 cut(s) 265
EcoT22I ATGCAT 1 cut(s) 662
FaeI CATG 4 cut(s) 421, 503, 664, 673
FaiI YATR 7 cut(s) 8, 137, 368, 419, 501, 662, 671
FaqI GGGAC 3 cut(s) 247, 378, 428
FatI CATG 4 cut(s) 417, 499, 660, 669
FokI GGATG 3 cut(s) 323, 617, 669
FspBI CTAG 1 cut(s) 42
GlaI GCGC 2 cut(s) 195, 587
GsaI CCCAGC 1 cut(s) 261
HaeIII GGCC 2 cut(s) 97, 651
HhaI GCGC 2 cut(s) 196, 588
Hin1II CATG 4 cut(s) 421, 503, 664, 673
Hin6I GCGC 2 cut(s) 194, 586
HinP1I GCGC 2 cut(s) 194, 586
HindIII AAGCTT 1 cut(s) 90
HphI GGTGA 1 cut(s) 177
Hpy188I TCNGA 1 cut(s) 472
Hpy188III TCNNGA 6 cut(s) 42, 163, 395, 428, 437, 509
HpyAV CCTTC 5 cut(s) 192, 198, 326, 475, 595
HpyCH4III ACNGT 3 cut(s) 39, 280, 639
HpyCH4V TGCA 6 cut(s) 58, 293, 443, 608, 621, 660
HpyF10VI GCNNNNNNNGC 3 cut(s) 139, 657, 666
HpyF3I CTNAG 2 cut(s) 210, 351
Hsp92II CATG 4 cut(s) 421, 503, 664, 673
HspAI GCGC 2 cut(s) 194, 586
KflI GGGWCCC 1 cut(s) 261
Kzo9I GATC 3 cut(s) 157, 343, 484
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 9 cut(s) 9, 243, 252, 272, 279, 361, 413, 526, 662
LweI GCATC 4 cut(s) 345, 430, 595, 647
MaeI CTAG 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 401
MalI GATC 3 cut(s) 159, 345, 486
MboI GATC 3 cut(s) 157, 343, 484
MboII GAAGA 3 cut(s) 310, 544, 559
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 2 cut(s) 72, 307
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 4 cut(s) 276, 349, 513, 514
Mph1103I ATGCAT 1 cut(s) 662
MroXI GAANNNNTTC 1 cut(s) 536
MseI TTAA 5 cut(s) 107, 201, 318, 327, 495
MspR9I CCNGG 1 cut(s) 267
MvaI CCWGG 1 cut(s) 267
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 3 cut(s) 139, 657, 666
NdeII GATC 3 cut(s) 157, 343, 484
NlaIII CATG 4 cut(s) 421, 503, 664, 673
NlaIV GGNNCC 2 cut(s) 262, 263
NsiI ATGCAT 1 cut(s) 662
NspI RCATGY 2 cut(s) 421, 503
PdmI GAANNNNTTC 1 cut(s) 536
PpuMI RGGWCCY 1 cut(s) 261
Psp5II RGGWCCY 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 265
PspFI CCCAGC 1 cut(s) 257
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 2 cut(s) 262, 263
PspPI GGNCC 1 cut(s) 261
PspPPI RGGWCCY 1 cut(s) 261
SaqAI TTAA 5 cut(s) 107, 201, 318, 327, 495
Sau3AI GATC 3 cut(s) 157, 343, 484
Sau96I GGNCC 1 cut(s) 261
ScrFI CCNGG 1 cut(s) 267
SduI GDGCHC 1 cut(s) 135
SetI ASST 9 cut(s) 28, 94, 190, 226, 239, 291, 325, 530, 627
SfaNI GCATC 4 cut(s) 345, 430, 595, 647
SinI GGWCC 1 cut(s) 261
SmlI CTYRAG 2 cut(s) 517, 645
SmoI CTYRAG 2 cut(s) 517, 645
Sse9I AATT 2 cut(s) 72, 307
SsiI CCGC 1 cut(s) 363
SspI AATATT 1 cut(s) 617
SspMI CTAG 1 cut(s) 42
StyD4I CCNGG 1 cut(s) 265
TaaI ACNGT 3 cut(s) 39, 280, 639
TasI AATT 2 cut(s) 72, 307
Tru1I TTAA 5 cut(s) 107, 201, 318, 327, 495
Tru9I TTAA 5 cut(s) 107, 201, 318, 327, 495
TscAI CASTG 2 cut(s) 285, 309
TspDTI ATGAA 2 cut(s) 567, 677
TspGWI ACGGA 2 cut(s) 481, 585
TspRI CASTG 2 cut(s) 285, 309
VpaK11BI GGWCC 1 cut(s) 261
XapI RAATTY 1 cut(s) 72
XbaI TCTAGA 1 cut(s) 41
XceI RCATGY 2 cut(s) 421, 503
XmnI GAANNNNTTC 1 cut(s) 536
XspI CTAG 1 cut(s) 42
Zsp2I ATGCAT 1 cut(s) 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.