Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0006998.1

Orthogroup ID

OG0003782

Physical Location & Seq

Genomic Coordinates Reverse (-)

1050 .. 6549

Gene Structure 5500 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3528 bp

atgggtgtgtctgatccaagccaactccacttgaagaaagagctcacccagattcgaaaggccgcccgcgttctccgcgatccggggaccacttcttcttggcgctctcctttggcttcctctagatctgctgcggcccccggagcggcggcggttccttctgcttgttccgcttcccacaataccaacaatggcggcggcggcgacaacaagagggtgttcttgtacaactggaagggctcttccaaatcgtcgagaaatgatgactacgaagaggatgaggaggaggatgattacggtgtcgaagatgaggacgctgcttcttcttctgttgcggcattcagtgtggatgacagcttgagccacggagattcccggagtgacactggtggtggtcagagccggtcgttgatgatgctccggcgcagatatccgaatcttctaccgccgctcaaagatggtagtggtaatggtaatggtaagaaagcaagtaagaaaatcgagtcccattctcatgttttgtccaaacatcagcagaaagagttgattcttggtaggaacttaagaaagggaggccgggatgagcaatccgatgacactgaggattactctaattctgaggatctgaggcacattagaggggcttccccattgctcttgaagcttaggcacaagcattggcctccggcctccactaatgttaggagaaatgatagtagagtagaggactcttcttattgctatagcactcctgcattgtcgactagttcttataataggtatggtgttcggaatccaagtactgttggatcttgggatggaactgcgacttcaatgaatgatggggatgatgaggttgaggaccatttggatattccgggtagcaaaggatgtgggattccttgttattggtccaaaaagaccccgaagcacagaggcatgtatggaagctgttgctccccttcgctttctgatactataaggagaaaaggaagtgtccttttgtgtggaagtcaaaatgtttatcctagacgacgggggtcgtcgtcaggttctaataagcggagaattgcatctagaagtgcgcaaggtgttctgccattgctaaacaatagtggtgatggtagaggagggtcatccataggaactgggaggagtgatgacgaactctccacaaactttggggaacttgatttggaagctttgagtagattggatggacggagatggtcaagctgccgaagtcaagaaggcttggagattgtagctctgagtggagtgggggaagaggaaagcactccagatagtgttagaagttttagccagaagtacaagccgatgttctttcgtgaattgattggccagaatattgtggtgcaatcacttataaatgctattgataggtgtaggattgctcccgtttatcttttccagggaccccgtggaactggaaaaacgtcaacagctagaatttttgctgcttccttgaattgcctagctcctgatgaaactaaaccctgtggctactgcagggagtgcactgattttatgtctggtaagaacaaggatctattggaagttaatggtaccaataagaaaggaattgataaagtcaggtacctattgaaaactctttctgtggctccctcatcagcatcatcaaggtataaggtttttgtgattgatgagtgtcaccttttgccttctaagacatggttggcatttcttaaatatttggaagaaccaccgcagcgagttgtcttcatattcattacaactgatttggataatgttcctcgtaccatacaatctcggtgtcagaaatatctcttcaacaaaattaaggatagtgagattgtggccaggttgaggaaaattgctgttgaagagaatctagatgttgaaccagatgcattagatttgattgctttgaatgcagatggttcacttcgagatgcagaaactatgttggaccagttgagtttgctggggaaaagaatttcaacatctcttgtgaatgaacttgtgggagttgtttccgatgacaagttactagatcttttggaattagcaatgtcatcagacactgcagaaacagtgaaaagggccagagatttgatggactctggggttgatcccatggttttgatgtctcagttggccagcttaattatggacatcattgcgggaaccaacggtactaatgatgtcaaacatgactccttatatggtgctcggaacttgaccgaaggagaattggagagattaaaacatgctctgaagattctttcggaggctgaaaagcagcttagggtttcaagtgagcgctcaacatggtttacagctactctgttgcagcttggttcagtgccttctccagaccttagtcactcatgcagcaggaggcatagctgcaagacaactgaggatgattcttcaagtgcttcaacttcaagagaagcagccacttacaaacagaagttagatggtcactatatactacaaaagtcaactcaacatgcttctgtacaaagagcaccaaatgacaattccaaccatcagggagattcgttgtcaagaaataatggtttcggtgtgaataccaagccctcacatgatcagtttgtggataatggggcctcgactcccttgtgtggaaatgttatggctggaaatatgagccttagatgtgtaaactctgaaaagttaaatgatgtatgggcacagtgtattgagaagtgtcattcaaagacattgaggcagctactacattctcatggaaagctagtgtctatatctgaagctgaaggtgttctggttgcttacgttgcatttgaagatggaagtatcaaatctagagcagagaggtttgtgagcagtatcacaaattcaatggaaatagttctcagacgcaatgtcgaggttagaattgttcacttgccaggtggggaggcttctctaaatggtccaagtccagttcacttgctcgaaggtctaaagcaggcaggaactatggcagaacttgtcagggaaaggaaaagagttggttcaaatgccacagatggttattccaattgttctctgtttctggatgggacccacaagtcaacctctgattcgtcagacttagttgcagatggcaatgctcaaacaagtgatacaaaagagagcggacaggagatcccaatgcagagaatagaatccattattcgcgaccaaaggttagagactgcgtggcttcaggttgcagagaaaggcacccctggttctttgagtcgtttgaaacctgaaaagaatcaggtcctgcctcaggatggcatttattacgaagatcagatggaatcgatgaattcaatgggattatcctctcagcaatgggatgatggattaaaccatgaagtaaaaatcttgagtgctaacagtgggaaggtcattcaacaggaccaaataggtaaaagagttgatcattatcctatgtcgccgagtttactacatgaaaagggcaagtccgacaaagatcacctgtga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005663 GO:0005694 GO:0005886 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0016020 GO:0016043 GO:0030154 GO:0032502 GO:0032989 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0046483 GO:0048468 GO:0048856 GO:0048869 GO:0050896 GO:0051716 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0090558 GO:0090626 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DNA_pol3_delta2 DNA_pol3_gamma3

Protein Analysis

1175

Amino Acids

128.82

Weight (kDa)

6.69

Isoelectric Point (pI)

49.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol3_delta2	PF13177	455 - 614	1.8e-34	DNA polymerase III, delta subunit
AAA	PF00004	476 - 611	5.7e-09	ATPase family associated with various cellular activities (AAA)
DNAX_ATPase_lid	PF22608	618 - 659	9.7e-10	DNA polymerase III clamp loader subunit, ATPase lid domain
DNA_pol3_gamma3	PF12169	668 - 788	3.1e-08	DNA polymerase III subunits gamma and tau domain III
DnaA_N-like_STI	PF23007	896 - 977	5.9e-31	STICHEL, DnaA_N-like alpha-beta domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003782)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14460 AT2G02480 AT2G02480
fragaria_vesca	FvH4_5g27450 FvH4_5g27450
malus_domestica	MD08G1249500.v1.1
prunus_persica	Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1 Prupe.1G586700_v2.0.a1
pyrus_communis	pycom08g21590
rosa_chinensis	RchiOBHm_Chr2g0146641 RchiOBHm_Chr7g0218871
rosa_laevigata	RLG00000001635 RLG00000002346 RLG00000002355
rosa_multiflora	Rmu_sc0003823.1_g000001 Rmu_sc0006998.1_g000001
rosa_roxburghii	Rroxscaffold_3G00240440
rosa_rugosa	Rorug07G0179300
rosa_samantha	Rh3AG021300 Rh3CG020600 Rh5BG537900 Rh5CG561800 Rh7AG322600 Rh7BG313700 Rh7CG339400 Rh7DG319800 Rh7DG389400
rosa_wichuraiana	Rw5G027500 Rw7G026930 Rw7G027310 Rw7G028430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	774, 1388
AasI	GACNNNNNNGTC	1 cut(s)	3103
Acc16I	TGCGCA	1 cut(s)	1086
Acc65I	GGTACC	2 cut(s)	1586, 1617
AccB1I	GGYRCC	3 cut(s)	1586, 1617, 3257
AccBSI	CCGCTC	3 cut(s)	146, 451, 3171
AccI	GTMKAC	1 cut(s)	761
AccII	CGCG	3 cut(s)	69, 78, 3213
AclWI	GGATC	7 cut(s)	8, 74, 630, 817, 1575, 2129, 3175
AcoI	YGGCCR	3 cut(s)	1360, 1860, 2160
AcsI	RAATTY	4 cut(s)	1470, 1998, 2887, 3349
AcuI	CTGAAG	4 cut(s)	2300, 2820, 2826, 3224
AfaI	GTAC	8 cut(s)	227, 802, 1331, 1588, 1619, 1801, 2200, 2529
AfeI	AGCGCT	1 cut(s)	2327
AfiI	CCNNNNNNNGG	6 cut(s)	82, 145, 1869, 2654, 3310, 3314
AflII	CTTAAG	1 cut(s)	562
AhdI	GACNNNNNGTC	1 cut(s)	2915
AhlI	ACTAGT	1 cut(s)	764
AjnI	CCWGG	4 cut(s)	1431, 1862, 2941, 3262
AjuI	GAANNNNNNNTTGG	2 cut(s)	2533, 2565
Alw21I	GWGCWC	4 cut(s)	45, 1541, 2236, 2539
Alw26I	GTCTC	2 cut(s)	2157, 3221
Alw44I	GTGCAC	1 cut(s)	1537
AlwI	GGATC	7 cut(s)	8, 74, 630, 817, 1575, 2129, 3175
AlwNI	CAGNNNCTG	2 cut(s)	2087, 3304
Aor51HI	AGCGCT	1 cut(s)	2327
ApaLI	GTGCAC	1 cut(s)	1537
ApoI	RAATTY	4 cut(s)	1470, 1998, 2887, 3349
Asp700I	GAANNNNTTC	2 cut(s)	2811, 2901
Asp718I	GGTACC	2 cut(s)	1586, 1617
AspLEI	GCGC	4 cut(s)	105, 426, 1087, 2328
AsuC2I	CCSGG	5 cut(s)	84, 141, 376, 578, 879
AsuHPI	GGTGA	4 cut(s)	37, 1130, 1685, 3512
AsuII	TTCGAA	1 cut(s)	55
AvaII	GGWCC	9 cut(s)	87, 862, 912, 1436, 1972, 2966, 3096, 3301, 3442
AxyI	CCTNAGG	1 cut(s)	3309
BaeGI	GKGCMC	2 cut(s)	1541, 2725
BalI	TGGCCA	3 cut(s)	1362, 1862, 2162
BanI	GGYRCC	3 cut(s)	1586, 1617, 3257
BanII	GRGCYC	2 cut(s)	45, 242
BbsI	GAAGAC	1 cut(s)	1753
Bbv12I	GWGCWC	4 cut(s)	45, 1541, 2236, 2539
BciT130I	CCWGG	4 cut(s)	1433, 1864, 2943, 3264
BclI	TGATCA	2 cut(s)	2617, 3463
BcnI	CCSGG	5 cut(s)	84, 141, 376, 578, 879
BcoDI	GTCTC	2 cut(s)	2157, 3221
BcuI	ACTAGT	1 cut(s)	764
BfmI	CTRYAG	3 cut(s)	742, 1528, 2088
BfoI	RGCGCY	2 cut(s)	106, 2329
BfrI	CTTAAG	1 cut(s)	562
BglII	AGATCT	2 cut(s)	125, 2056
BmcAI	AGTACT	1 cut(s)	802
Bme1390I	CCNGG	9 cut(s)	84, 141, 376, 578, 879, 1433, 1864, 2943, 3264
Bme18I	GGWCC	9 cut(s)	87, 862, 912, 1436, 1972, 2966, 3096, 3301, 3442
BmeRI	GACNNNNNGTC	1 cut(s)	2915
BmrFI	CCNGG	9 cut(s)	84, 141, 376, 578, 879, 1433, 1864, 2943, 3264
BmrI	ACTGGG	1 cut(s)	1158
BmsI	GCATC	5 cut(s)	405, 1082, 1664, 1900, 1945
BmuI	ACTGGG	1 cut(s)	1158
BoxI	GACNNNNGTC	2 cut(s)	1039, 2385
BpiI	GAAGAC	1 cut(s)	1753
BplI	GAGNNNNNCTC	2 cut(s)	593, 625
BpmI	CTGGAG	2 cut(s)	1284, 2361
Bpu10I	CCTNAGC	2 cut(s)	665, 2309
Bpu14I	TTCGAA	1 cut(s)	55
BpuEI	CTTGAG	2 cut(s)	379, 3430
BpuMI	CCSGG	5 cut(s)	84, 141, 376, 578, 879
Bsa29I	ATCGAT	1 cut(s)	3344
BsaBI	GATNNNNATC	1 cut(s)	3468
BsaJI	CCNNGG	7 cut(s)	83, 139, 364, 1432, 1441, 2139, 3262
BsaXI	ACNNNNNCTCC	2 cut(s)	1154, 1184
Bsc4I	CCNNNNNNNGG	6 cut(s)	82, 145, 1869, 2654, 3310, 3314
Bse118I	RCCGGY	1 cut(s)	402
Bse1I	ACTGG	6 cut(s)	236, 391, 1153, 1453, 1975, 2975
Bse21I	CCTNAGG	1 cut(s)	3309
Bse3DI	GCAATG	7 cut(s)	650, 1100, 2079, 2181, 2920, 3148, 3380
Bse8I	GATNNNNATC	1 cut(s)	3468
BseBI	CCWGG	4 cut(s)	1433, 1864, 2943, 3264
BseCI	ATCGAT	1 cut(s)	3344
BseDI	CCNNGG	7 cut(s)	83, 139, 364, 1432, 1441, 2139, 3262
BseJI	GATNNNNATC	1 cut(s)	3468
BseLI	CCNNNNNNNGG	6 cut(s)	82, 145, 1869, 2654, 3310, 3314
BseMI	GCAATG	7 cut(s)	650, 1100, 2079, 2181, 2920, 3148, 3380
BseMII	CTCAG	9 cut(s)	591, 609, 617, 1262, 2168, 2415, 2920, 3323, 3383
BseNI	ACTGG	6 cut(s)	236, 391, 1153, 1453, 1975, 2975
BseRI	GAGGAG	4 cut(s)	296, 299, 1143, 1168
BseSI	GKGCMC	2 cut(s)	1541, 2725
BseYI	CCCAGC	1 cut(s)	1987
Bsh1236I	CGCG	3 cut(s)	69, 78, 3213
Bsh1285I	CGRYCG	1 cut(s)	407
BshNI	GGYRCC	3 cut(s)	1586, 1617, 3257
BshVI	ATCGAT	1 cut(s)	3344
BsiEI	CGRYCG	1 cut(s)	407
BsiHKAI	GWGCWC	4 cut(s)	45, 1541, 2236, 2539
BsiSI	CCGG	8 cut(s)	83, 141, 376, 403, 421, 577, 686, 878
BslFI	GGGAC	4 cut(s)	100, 490, 1449, 3109
BslI	CCNNNNNNNGG	6 cut(s)	82, 145, 1869, 2654, 3310, 3314
BsmAI	GTCTC	2 cut(s)	2157, 3221
BsmFI	GGGAC	4 cut(s)	100, 490, 1449, 3109
BsmI	GAATGC	2 cut(s)	338, 1939
Bsp119I	TTCGAA	1 cut(s)	55
Bsp1286I	GDGCHC	6 cut(s)	45, 242, 1541, 2236, 2539, 2725
Bsp1407I	TGTACA	2 cut(s)	225, 2527
Bsp19I	CCATGG	1 cut(s)	2139
Bsp68I	TCGCGA	1 cut(s)	3213
BspCNI	CTCAG	9 cut(s)	592, 610, 618, 1263, 2167, 2416, 2919, 3322, 3382
BspDI	ATCGAT	1 cut(s)	3344
BspFNI	CGCG	3 cut(s)	69, 78, 3213
BspMAI	CTGCAG	2 cut(s)	1532, 2092
BspPI	GGATC	7 cut(s)	8, 74, 630, 817, 1575, 2129, 3175
BspQI	GCTCTTC	1 cut(s)	247
BspT104I	TTCGAA	1 cut(s)	55
BspT107I	GGYRCC	3 cut(s)	1586, 1617, 3257
BspTI	CTTAAG	1 cut(s)	562
BsrBI	CCGCTC	3 cut(s)	146, 451, 3171
BsrDI	GCAATG	7 cut(s)	650, 1100, 2079, 2181, 2920, 3148, 3380
BsrFI	RCCGGY	1 cut(s)	402
BsrGI	TGTACA	2 cut(s)	225, 2527
BsrI	ACTGG	6 cut(s)	236, 391, 1153, 1453, 1975, 2975
BssAI	RCCGGY	1 cut(s)	402
BssECI	CCNNGG	7 cut(s)	83, 139, 364, 1432, 1441, 2139, 3262
BssT1I	CCWWGG	1 cut(s)	2139
Bst2UI	CCWGG	4 cut(s)	1433, 1864, 2943, 3264
Bst4CI	ACNGT	6 cut(s)	299, 805, 2098, 2198, 2727, 3422
Bst6I	CTCTTC	6 cut(s)	247, 267, 736, 1281, 1835, 1881
BstAFI	CTTAAG	1 cut(s)	562
BstAPI	GCANNNNNTGC	1 cut(s)	1536
BstAUI	TGTACA	2 cut(s)	225, 2527
BstBI	TTCGAA	1 cut(s)	55
BstC8I	GCNNGC	3 cut(s)	67, 2164, 3003
BstDSI	CCRYGG	3 cut(s)	364, 1441, 2139
BstENI	CCTNNNNNAGG	1 cut(s)	3308
BstFNI	CGCG	3 cut(s)	69, 78, 3213
BstH2I	RGCGCY	2 cut(s)	106, 2329
BstHHI	GCGC	4 cut(s)	105, 426, 1087, 2328
BstMAI	GTCTC	2 cut(s)	2157, 3221
BstMCI	CGRYCG	1 cut(s)	407
BstMWI	GCNNNNNNNGC	8 cut(s)	75, 143, 170, 201, 661, 1536, 1934, 2828
BstNI	CCWGG	4 cut(s)	1433, 1864, 2943, 3264
BstNSI	RCATGY	3 cut(s)	943, 2276, 2522
BstPAI	GACNNNNGTC	2 cut(s)	1039, 2385
BstSCI	CCNGG	9 cut(s)	82, 139, 374, 576, 877, 1431, 1862, 2941, 3262
BstSFI	CTRYAG	3 cut(s)	742, 1528, 2088
BstSLI	GKGCMC	2 cut(s)	1541, 2725
BstUI	CGCG	3 cut(s)	69, 78, 3213
BstV2I	GAAGAC	1 cut(s)	1753
BstX2I	RGATCY	6 cut(s)	125, 622, 809, 1567, 2056, 3180
BstYI	RGATCY	6 cut(s)	125, 622, 809, 1567, 2056, 3180
Bsu15I	ATCGAT	1 cut(s)	3344
Bsu36I	CCTNAGG	1 cut(s)	3309
BsuTUI	ATCGAT	1 cut(s)	3344
BtgI	CCRYGG	3 cut(s)	364, 1441, 2139
BtsI	GCAGTG	1 cut(s)	2085
BtsIMutI	CAGTG	9 cut(s)	349, 384, 597, 1539, 2085, 2103, 2373, 2732, 3427
BtuMI	TCGCGA	1 cut(s)	3213
Cac8I	GCNNGC	3 cut(s)	67, 2164, 3003
CaiI	CAGNNNCTG	2 cut(s)	2087, 3304
CfoI	GCGC	4 cut(s)	105, 426, 1087, 2328
Cfr10I	RCCGGY	1 cut(s)	402
ClaI	ATCGAT	1 cut(s)	3344
CseI	GACGC	2 cut(s)	323, 2919
Csp6I	GTAC	8 cut(s)	226, 801, 1330, 1587, 1618, 1800, 2199, 2528
CspCI	CAANNNNNGTGG	4 cut(s)	874, 909, 1162, 1197
CviQI	GTAC	8 cut(s)	226, 801, 1330, 1587, 1618, 1800, 2199, 2528
DrdI	GACNNNNNNGTC	1 cut(s)	3103
DriI	GACNNNNNGTC	1 cut(s)	2915
DseDI	GACNNNNNNGTC	1 cut(s)	3103
EaeI	YGGCCR	3 cut(s)	1360, 1860, 2160
Eam1104I	CTCTTC	6 cut(s)	247, 267, 736, 1281, 1835, 1881
Eam1105I	GACNNNNNGTC	1 cut(s)	2915
EarI	CTCTTC	6 cut(s)	247, 267, 736, 1281, 1835, 1881
Ecl136II	GAGCTC	1 cut(s)	43
Eco130I	CCWWGG	1 cut(s)	2139
Eco24I	GRGCYC	2 cut(s)	45, 242
Eco32I	GATATC	1 cut(s)	431
Eco47I	GGWCC	9 cut(s)	87, 862, 912, 1436, 1972, 2966, 3096, 3301, 3442
Eco47III	AGCGCT	1 cut(s)	2327
Eco53kI	GAGCTC	1 cut(s)	43
Eco57I	CTGAAG	4 cut(s)	2300, 2820, 2826, 3224
Eco81I	CCTNAGG	1 cut(s)	3309
EcoICRI	GAGCTC	1 cut(s)	43
EcoNI	CCTNNNNNAGG	1 cut(s)	3308
EcoO109I	RGGNCCY	4 cut(s)	1436, 2637, 3096, 3301
EcoRI	GAATTC	1 cut(s)	3349
EcoRII	CCWGG	4 cut(s)	1431, 1862, 2941, 3262
EcoRV	GATATC	1 cut(s)	431
EcoT14I	CCWWGG	1 cut(s)	2139
EcoT22I	ATGCAT	1 cut(s)	1915
EcoT38I	GRGCYC	2 cut(s)	45, 242
ErhI	CCWWGG	1 cut(s)	2139
FaqI	GGGAC	4 cut(s)	100, 490, 1449, 3109
FauI	CCCGC	2 cut(s)	74, 2179
FbaI	TGATCA	2 cut(s)	2617, 3463
FblI	GTMKAC	1 cut(s)	761
FriOI	GRGCYC	2 cut(s)	45, 242
FspI	TGCGCA	1 cut(s)	1086
GlaI	GCGC	4 cut(s)	104, 425, 1086, 2327
GsaI	CCCAGC	1 cut(s)	1991
GsuI	CTGGAG	2 cut(s)	1284, 2361
HaeII	RGCGCY	2 cut(s)	106, 2329
HapII	CCGG	8 cut(s)	83, 141, 376, 403, 421, 577, 686, 878
HgaI	GACGC	2 cut(s)	323, 2919
HhaI	GCGC	4 cut(s)	105, 426, 1087, 2328
Hin6I	GCGC	4 cut(s)	103, 424, 1085, 2326
HinP1I	GCGC	4 cut(s)	103, 424, 1085, 2326
HincII	GTYRAC	4 cut(s)	762, 1461, 2511, 3108
HindII	GTYRAC	4 cut(s)	762, 1461, 2511, 3108
HindIII	AAGCTT	2 cut(s)	662, 1200
HpaII	CCGG	8 cut(s)	83, 141, 376, 403, 421, 577, 686, 878
HphI	GGTGA	4 cut(s)	37, 1130, 1685, 3512
Hpy99I	CGWCG	3 cut(s)	256, 1038, 1048
HpyCH4III	ACNGT	6 cut(s)	299, 805, 2098, 2198, 2727, 3422
HpyCH4IV	ACGT	2 cut(s)	1457, 2826
HpyF10VI	GCNNNNNNNGC	8 cut(s)	75, 143, 170, 201, 661, 1536, 1934, 2828
HpySE526I	ACGT	2 cut(s)	1457, 2826
HspAI	GCGC	4 cut(s)	103, 424, 1085, 2326
KflI	GGGWCCC	2 cut(s)	1436, 3096
KpnI	GGTACC	2 cut(s)	1590, 1621
Ksp22I	TGATCA	2 cut(s)	2617, 3463
LguI	GCTCTTC	1 cut(s)	247
LmnI	GCTCC	6 cut(s)	143, 423, 962, 1420, 1504, 1648
LweI	GCATC	5 cut(s)	405, 1082, 1664, 1900, 1945
MaeII	ACGT	2 cut(s)	1457, 2826
MaeIII	GTNAC	5 cut(s)	380, 1691, 2049, 2387, 2489
MbiI	CCGCTC	3 cut(s)	146, 451, 3171
MfeI	CAATTG	1 cut(s)	3073
MflI	RGATCY	6 cut(s)	125, 622, 809, 1567, 2056, 3180
MhlI	GDGCHC	6 cut(s)	45, 242, 1541, 2236, 2539, 2725
MlsI	TGGCCA	3 cut(s)	1362, 1862, 2162
MluNI	TGGCCA	3 cut(s)	1362, 1862, 2162
MlyI	GAGTC	6 cut(s)	512, 722, 2117, 2213, 2638, 3283
MmeI	TCCRAC	3 cut(s)	787, 1950, 2577
Mox20I	TGGCCA	3 cut(s)	1362, 1862, 2162
Mph1103I	ATGCAT	1 cut(s)	1915
MroXI	GAANNNNTTC	2 cut(s)	2811, 2901
MscI	TGGCCA	3 cut(s)	1362, 1862, 2162
MseI	TTAA	8 cut(s)	563, 1581, 1728, 1842, 2168, 2267, 2708, 3389
MslI	CAYNNNNRTG	2 cut(s)	513, 2775
Msp20I	TGGCCA	3 cut(s)	1362, 1862, 2162
MspCI	CTTAAG	1 cut(s)	562
MspI	CCGG	8 cut(s)	83, 141, 376, 403, 421, 577, 686, 878
MspR9I	CCNGG	9 cut(s)	84, 141, 376, 578, 879, 1433, 1864, 2943, 3264
MunI	CAATTG	1 cut(s)	3073
Mva1269I	GAATGC	2 cut(s)	338, 1939
MvaI	CCWGG	4 cut(s)	1433, 1864, 2943, 3264
MvnI	CGCG	3 cut(s)	69, 78, 3213
MwoI	GCNNNNNNNGC	8 cut(s)	75, 143, 170, 201, 661, 1536, 1934, 2828
NciI	CCSGG	5 cut(s)	84, 141, 376, 578, 879
NcoI	CCATGG	1 cut(s)	2139
NmeAIII	GCCGAG	1 cut(s)	3507
NmuCI	GTSAC	4 cut(s)	380, 1691, 2387, 2489
NruI	TCGCGA	1 cut(s)	3213
NsbI	TGCGCA	1 cut(s)	1086
NsiI	ATGCAT	1 cut(s)	1915
NspI	RCATGY	3 cut(s)	943, 2276, 2522
NspV	TTCGAA	1 cut(s)	55
PciSI	GCTCTTC	1 cut(s)	247
PctI	GAATGC	2 cut(s)	338, 1939
PdmI	GAANNNNTTC	2 cut(s)	2811, 2901
PfoI	TCCNGGA	1 cut(s)	374
PleI	GAGTC	6 cut(s)	511, 722, 2117, 2213, 2638, 3282
PpsI	GAGTC	6 cut(s)	511, 722, 2117, 2213, 2638, 3282
PpuMI	RGGWCCY	3 cut(s)	1436, 3096, 3301
PshAI	GACNNNNGTC	2 cut(s)	1039, 2385
PsiI	TTATAA	2 cut(s)	774, 1388
Psp124BI	GAGCTC	1 cut(s)	45
Psp5II	RGGWCCY	3 cut(s)	1436, 3096, 3301
Psp6I	CCWGG	4 cut(s)	1431, 1862, 2941, 3262
PspFI	CCCAGC	1 cut(s)	1987
PspGI	CCWGG	4 cut(s)	1431, 1862, 2941, 3262
PspPPI	RGGWCCY	3 cut(s)	1436, 3096, 3301
PsrI	GAACNNNNNNTAC	2 cut(s)	2182, 2214
PstI	CTGCAG	2 cut(s)	1532, 2092
PstNI	CAGNNNCTG	2 cut(s)	2087, 3304
PsuI	RGATCY	6 cut(s)	125, 622, 809, 1567, 2056, 3180
RruI	TCGCGA	1 cut(s)	3213
RsaI	GTAC	8 cut(s)	227, 802, 1331, 1588, 1619, 1801, 2200, 2529
RsaNI	GTAC	8 cut(s)	226, 801, 1330, 1587, 1618, 1800, 2199, 2528
RseI	CAYNNNNRTG	2 cut(s)	513, 2775
SacI	GAGCTC	1 cut(s)	45
SalI	GTCGAC	1 cut(s)	760
SapI	GCTCTTC	1 cut(s)	247
SaqAI	TTAA	8 cut(s)	563, 1581, 1728, 1842, 2168, 2267, 2708, 3389
ScaI	AGTACT	1 cut(s)	802
SchI	GAGTC	6 cut(s)	512, 722, 2117, 2213, 2638, 3283
ScrFI	CCNGG	9 cut(s)	84, 141, 376, 578, 879, 1433, 1864, 2943, 3264
SduI	GDGCHC	6 cut(s)	45, 242, 1541, 2236, 2539, 2725
SfaNI	GCATC	5 cut(s)	405, 1082, 1664, 1900, 1945
SfcI	CTRYAG	3 cut(s)	742, 1528, 2088
SfuI	TTCGAA	1 cut(s)	55
SinI	GGWCC	9 cut(s)	87, 862, 912, 1436, 1972, 2966, 3096, 3301, 3442
SmiMI	CAYNNNNRTG	2 cut(s)	513, 2775
SmlI	CTYRAG	3 cut(s)	358, 562, 3409
SmoI	CTYRAG	3 cut(s)	358, 562, 3409
SpeI	ACTAGT	1 cut(s)	764
SspI	AATATT	2 cut(s)	1369, 1733
SstI	GAGCTC	1 cut(s)	45
StyD4I	CCNGG	9 cut(s)	82, 139, 374, 576, 877, 1431, 1862, 2941, 3262
StyI	CCWWGG	1 cut(s)	2139
TaaI	ACNGT	6 cut(s)	299, 805, 2098, 2198, 2727, 3422
TaiI	ACGT	2 cut(s)	1460, 2829
TaqII	GACCGA	1 cut(s)	2261
TatI	WGTACW	4 cut(s)	225, 800, 1329, 2527
TauI	GCSGC	9 cut(s)	65, 137, 149, 152, 198, 201, 204, 338, 451
Tru1I	TTAA	8 cut(s)	563, 1581, 1728, 1842, 2168, 2267, 2708, 3389
Tru9I	TTAA	8 cut(s)	563, 1581, 1728, 1842, 2168, 2267, 2708, 3389
TscAI	CASTG	9 cut(s)	349, 391, 604, 1546, 2092, 2103, 2373, 2732, 3427
TseFI	GTSAC	4 cut(s)	380, 1691, 2387, 2489
Tsp45I	GTSAC	4 cut(s)	380, 1691, 2387, 2489
TspDTI	ATGAA	8 cut(s)	851, 1521, 1753, 1759, 2034, 3362, 3411, 3510
TspGWI	ACGGA	2 cut(s)	381, 1237
TspRI	CASTG	9 cut(s)	349, 391, 604, 1546, 2092, 2103, 2373, 2732, 3427
Vha464I	CTTAAG	1 cut(s)	562
VneI	GTGCAC	1 cut(s)	1537
VpaK11BI	GGWCC	9 cut(s)	87, 862, 912, 1436, 1972, 2966, 3096, 3301, 3442
XagI	CCTNNNNNAGG	1 cut(s)	3308
XapI	RAATTY	4 cut(s)	1470, 1998, 2887, 3349
XbaI	TCTAGA	4 cut(s)	122, 1076, 1894, 2855
XceI	RCATGY	3 cut(s)	943, 2276, 2522
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1439, 2116, 2170
XmiI	GTMKAC	1 cut(s)	761
XmnI	GAANNNNTTC	2 cut(s)	2811, 2901
ZrmI	AGTACT	1 cut(s)	802
Zsp2I	ATGCAT	1 cut(s)	1915

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.