Rmu_ssc0000015.1_g000012

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000015.1
OG0005219
Physical Location & Seq
Forward (+)
68697 .. 69086
390 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 390 bp
atgaacttcatctgcattccctgtcaggtcttccattgttttggtcaatacttctgcctccattttgaagccttccagcttggcatggctcctgtttatatggcttttctacgttttatgggtgatgagaatgaggcacggaactacagctacagcctcgaggttggagcaaatggcaggaaactcatatgggagggcaccccaagaagtgtccgtgatagccaccggaaggtaagggatagccatgacggtctcattattcaacgaaacatggctttatttttctctggaggagataggaaggagcttaagctgagagttaccgggaggatatggaaggaacagcaaaatccagatgctggagtgtgcataccaaacttatgtagctaa

Protein Analysis

129

Amino Acids

14.9

Weight (kDa)

8.59

Isoelectric Point (pI)

21.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sina_TRAF PF03145 11 - 108 1.5e-35 Sina, TRAF-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 197
AccB7I CCANNNNNTGG 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 229, 359
AflII CTTAAG 1 cut(s) 308
AgsI TTSAA 2 cut(s) 68, 263
AluBI AGCT 5 cut(s) 79, 150, 307, 313, 387
AluI AGCT 5 cut(s) 79, 150, 307, 313, 387
Alw26I GTCTC 1 cut(s) 257
AlwNI CAGNNNCTG 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 158
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 1 cut(s) 134
AvaI CYCGRG 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 200
BanI GGYRCC 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 22
BcnI CCSGG 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 257
BfmI CTRYAG 2 cut(s) 145, 151
BfrI CTTAAG 1 cut(s) 308
Bme1390I CCNGG 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 158
BmiI GGNNCC 2 cut(s) 90, 199
BmrFI CCNGG 1 cut(s) 325
BmsI GCATC 1 cut(s) 346
BpiI GAAGAC 1 cut(s) 22
BpmI CTGGAG 2 cut(s) 309, 381
BpuMI CCSGG 1 cut(s) 325
BsaI GGTCTC 1 cut(s) 257
BsaWI WCCGGW 1 cut(s) 225
Bsc4I CCNNNNNNNGG 2 cut(s) 229, 359
BseLI CCNNNNNNNGG 2 cut(s) 229, 359
BseMII CTCAG 1 cut(s) 305
BseRI GAGGAG 1 cut(s) 306
BseSI GKGCMC 1 cut(s) 200
BshNI GGYRCC 1 cut(s) 197
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 2 cut(s) 226, 324
BslI CCNNNNNNNGG 2 cut(s) 229, 359
BsmAI GTCTC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 15
Bso31I GGTCTC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 306
BspLI GGNNCC 2 cut(s) 90, 199
BspT107I GGYRCC 1 cut(s) 197
BspTI CTTAAG 1 cut(s) 308
BspTNI GGTCTC 1 cut(s) 257
Bst4CI ACNGT 1 cut(s) 251
BstAFI CTTAAG 1 cut(s) 308
BstDEI CTNAG 1 cut(s) 314
BstMAI GTCTC 1 cut(s) 257
BstSCI CCNGG 1 cut(s) 323
BstSFI CTRYAG 2 cut(s) 145, 151
BstSLI GKGCMC 1 cut(s) 200
BstV2I GAAGAC 1 cut(s) 22
BstXI CCANNNNNNTGG 1 cut(s) 41
CaiI CAGNNNCTG 1 cut(s) 359
CviAII CATG 3 cut(s) 85, 245, 271
DdeI CTNAG 1 cut(s) 314
Eco31I GGTCTC 1 cut(s) 257
Eco88I CYCGRG 1 cut(s) 158
FaeI CATG 3 cut(s) 88, 248, 274
FatI CATG 3 cut(s) 84, 244, 270
FauNDI CATATG 1 cut(s) 188
GsuI CTGGAG 2 cut(s) 309, 381
HapII CCGG 2 cut(s) 226, 324
Hin1II CATG 3 cut(s) 88, 248, 274
HpaII CCGG 2 cut(s) 226, 324
HphI GGTGA 1 cut(s) 134
Hpy188III TCNNGA 2 cut(s) 288, 353
HpyAV CCTTC 4 cut(s) 82, 223, 295, 331
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 2 cut(s) 15, 369
HpyF3I CTNAG 1 cut(s) 314
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 3 cut(s) 88, 248, 274
LmnI GCTCC 3 cut(s) 94, 167, 304
LweI GCATC 1 cut(s) 346
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 1 cut(s) 319
MboII GAAGA 1 cut(s) 22
MhlI GDGCHC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 7 cut(s) 68, 127, 154, 167, 187, 284, 321
MseI TTAA 1 cut(s) 309
MspCI CTTAAG 1 cut(s) 308
MspI CCGG 2 cut(s) 226, 324
MspR9I CCNGG 1 cut(s) 325
Mva1269I GAATGC 1 cut(s) 15
NciI CCSGG 1 cut(s) 325
NdeI CATATG 1 cut(s) 188
NlaIII CATG 3 cut(s) 88, 248, 274
NlaIV GGNNCC 2 cut(s) 90, 199
PaeR7I CTCGAG 1 cut(s) 158
PctI GAATGC 1 cut(s) 15
PflMI CCANNNNNTGG 1 cut(s) 359
PspN4I GGNNCC 2 cut(s) 90, 199
PspXI VCTCGAGB 1 cut(s) 158
PsrI GAACNNNNNNTAC 2 cut(s) 134, 166
PstNI CAGNNNCTG 1 cut(s) 359
SaqAI TTAA 1 cut(s) 309
ScrFI CCNGG 1 cut(s) 325
SduI GDGCHC 1 cut(s) 200
SetI ASST 9 cut(s) 30, 81, 115, 152, 165, 234, 309, 315, 389
SfaNI GCATC 1 cut(s) 346
SfcI CTRYAG 2 cut(s) 145, 151
Sfr274I CTCGAG 1 cut(s) 158
SlaI CTCGAG 1 cut(s) 158
SmlI CTYRAG 2 cut(s) 158, 308
SmoI CTYRAG 2 cut(s) 158, 308
StyD4I CCNGG 1 cut(s) 323
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 1 cut(s) 115
TaqI TCGA 1 cut(s) 159
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 154, 203
Van91I CCANNNNNTGG 1 cut(s) 359
Vha464I CTTAAG 1 cut(s) 308
XhoI CTCGAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.