Rmu_ssc0000134.1_g000010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000134.1
OG0010620
Physical Location & Seq
Forward (+)
45151 .. 45777
627 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 627 bp
atggttttgaagttatattactgtgttttggacgaaaccctaattcagaaggacaccggatttaatcggctcaaagagctcacgttgtttgagactgattttgatggagtagtcgtggagctgctttctagatcccctaatcttgagatattgatactcaagccatctcgtggaagtgtagataaatatattgaactttgtgatcttcccaagctgaagaaggttgaattttggcctcaaggttatagctacaagattgaagcagccaatcttcaaactcttatacacaacgattgtatggcctttagcccgctgccgcacatcggagcactcaactgcggtaacataaaggagatcaaaatgtaccttggccattctaccgttaccaaagaatatattgtcaaacttatttctaagtttccatttcttgaggagttgatcttgcgcgcttccctctcatcaaaggagcagaaaggctcttgtcgtctggacgagttacaagattactttacttctaagtttccaaatcttcgagcttgccgcttaaggggttgttatgagcattatggcaattcaattagttcttttgttaaacatgaggaggagaacttggtttccaattattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

208

Amino Acids

23.99

Weight (kDa)

6.53

Isoelectric Point (pI)

53.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At5g56370 PF24758 2 - 96 5.8e-06 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 170
AccII CGCG 1 cut(s) 447
AciI CCGC 4 cut(s) 311, 317, 339, 541
AclWI GGATC 1 cut(s) 126
AcoI YGGCCR 1 cut(s) 370
AcsI RAATTY 1 cut(s) 227
AcuI CTGAAG 1 cut(s) 236
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 3 cut(s) 170, 323, 547
AflII CTTAAG 1 cut(s) 544
AgsI TTSAA 6 cut(s) 10, 194, 227, 260, 275, 576
AloI GAACNNNNNNTCC 1 cut(s) 599
AluBI AGCT 5 cut(s) 79, 121, 214, 249, 536
AluI AGCT 5 cut(s) 79, 121, 214, 249, 536
Alw21I GWGCWC 2 cut(s) 81, 331
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 126
AoxI GGCC 3 cut(s) 233, 300, 370
ApeKI GCWGC 3 cut(s) 121, 263, 313
ApoI RAATTY 1 cut(s) 227
AspLEI GCGC 2 cut(s) 447, 449
BalI TGGCCA 1 cut(s) 372
BanII GRGCYC 1 cut(s) 81
BarI GAAGNNNNNNTAC 1 cut(s) 34
BauI CACGAG 1 cut(s) 168
Bbv12I GWGCWC 2 cut(s) 81, 331
BbvI GCAGC 3 cut(s) 108, 275, 300
BccI CCATC 2 cut(s) 98, 172
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 1 cut(s) 129
BfrI CTTAAG 1 cut(s) 544
BisI GCNGC 5 cut(s) 122, 264, 314, 317, 541
BlsI GCNGC 5 cut(s) 123, 265, 315, 318, 542
BpuEI CTTGAG 4 cut(s) 143, 164, 222, 449
BsaJI CCNNGG 1 cut(s) 367
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 323, 547
BseDI CCNNGG 1 cut(s) 367
BseLI CCNNNNNNNGG 3 cut(s) 170, 323, 547
BsePI GCGCGC 1 cut(s) 445
BseRI GAGGAG 3 cut(s) 446, 614, 617
BseXI GCAGC 3 cut(s) 108, 275, 300
Bsh1236I CGCG 1 cut(s) 447
BshFI GGCC 3 cut(s) 235, 302, 372
BsiHKAI GWGCWC 2 cut(s) 81, 331
BsiSI CCGG 1 cut(s) 57
BslI CCNNNNNNNGG 3 cut(s) 170, 323, 547
BsmAI GTCTC 1 cut(s) 86
BsnI GGCC 3 cut(s) 235, 302, 372
Bsp1286I GDGCHC 2 cut(s) 81, 331
Bsp143I GATC 4 cut(s) 131, 202, 354, 438
BspACI CCGC 4 cut(s) 311, 317, 339, 541
BspANI GGCC 3 cut(s) 235, 302, 372
BspFNI CGCG 1 cut(s) 447
BspPI GGATC 1 cut(s) 126
BspTI CTTAAG 1 cut(s) 544
BssECI CCNNGG 1 cut(s) 367
BssHII GCGCGC 1 cut(s) 445
BssMI GATC 4 cut(s) 131, 202, 354, 438
BssSI CACGAG 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 367
Bst2BI CACGAG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 23, 382
BstAFI CTTAAG 1 cut(s) 544
BstC8I GCNNGC 3 cut(s) 311, 447, 538
BstDEI CTNAG 2 cut(s) 414, 516
BstFNI CGCG 1 cut(s) 447
BstHHI GCGC 2 cut(s) 447, 449
BstKTI GATC 4 cut(s) 134, 205, 357, 441
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 4 cut(s) 131, 202, 354, 438
BstMWI GCNNNNNNNGC 1 cut(s) 76
BstUI CGCG 1 cut(s) 447
BstV1I GCAGC 3 cut(s) 108, 275, 300
BstX2I RGATCY 1 cut(s) 131
BstYI RGATCY 1 cut(s) 131
BsuRI GGCC 3 cut(s) 235, 302, 372
Cac8I GCNNGC 3 cut(s) 311, 447, 538
CfoI GCGC 2 cut(s) 447, 449
Csp6I GTAC 1 cut(s) 364
CviAII CATG 1 cut(s) 596
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 2 cut(s) 414, 516
DpnI GATC 4 cut(s) 133, 204, 356, 440
DpnII GATC 4 cut(s) 131, 202, 354, 438
EaeI YGGCCR 1 cut(s) 370
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 367
Eco24I GRGCYC 1 cut(s) 81
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 236
EcoICRI GAGCTC 1 cut(s) 79
EcoT14I CCWWGG 1 cut(s) 367
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 1 cut(s) 599
FatI CATG 1 cut(s) 595
FauI CCCGC 1 cut(s) 318
Fnu4HI GCNGC 5 cut(s) 122, 264, 314, 317, 541
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 5 cut(s) 122, 264, 314, 317, 541
FspBI CTAG 1 cut(s) 129
GlaI GCGC 2 cut(s) 446, 448
GluI GCNGC 5 cut(s) 122, 264, 314, 317, 541
HaeIII GGCC 3 cut(s) 235, 302, 372
HapII CCGG 1 cut(s) 57
HhaI GCGC 2 cut(s) 447, 449
Hin1II CATG 1 cut(s) 599
Hin6I GCGC 2 cut(s) 445, 447
HinP1I GCGC 2 cut(s) 445, 447
HpaII CCGG 1 cut(s) 57
Hpy188I TCNGA 2 cut(s) 48, 326
Hpy188III TCNNGA 4 cut(s) 129, 143, 428, 488
HpyAV CCTTC 2 cut(s) 43, 214
HpyCH4III ACNGT 2 cut(s) 23, 382
HpyCH4IV ACGT 1 cut(s) 83
HpyF10VI GCNNNNNNNGC 1 cut(s) 76
HpyF3I CTNAG 2 cut(s) 414, 516
HpySE526I ACGT 1 cut(s) 83
Hsp92II CATG 1 cut(s) 599
HspAI GCGC 2 cut(s) 445, 447
Kzo9I GATC 4 cut(s) 131, 202, 354, 438
LmnI GCTCC 3 cut(s) 118, 326, 466
LpnPI CCDG 2 cut(s) 70, 473
Lsp1109I GCAGC 3 cut(s) 108, 275, 300
MaeI CTAG 1 cut(s) 129
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 3 cut(s) 341, 382, 495
MalI GATC 4 cut(s) 133, 204, 356, 440
MboI GATC 4 cut(s) 131, 202, 354, 438
MboII GAAGA 4 cut(s) 197, 229, 263, 521
MflI RGATCY 1 cut(s) 131
MhlI GDGCHC 2 cut(s) 81, 331
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 5 cut(s) 42, 227, 571, 576, 619
MluNI TGGCCA 1 cut(s) 372
MnlI CCTC 5 cut(s) 246, 424, 464, 592, 595
Mox20I TGGCCA 1 cut(s) 372
MscI TGGCCA 1 cut(s) 372
MseI TTAA 3 cut(s) 63, 545, 591
Msp20I TGGCCA 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 313
MspCI CTTAAG 1 cut(s) 544
MspI CCGG 1 cut(s) 57
MvnI CGCG 1 cut(s) 447
MwoI GCNNNNNNNGC 1 cut(s) 76
NdeII GATC 4 cut(s) 131, 202, 354, 438
NlaIII CATG 1 cut(s) 599
PauI GCGCGC 1 cut(s) 445
PflMI CCANNNNNTGG 1 cut(s) 170
PkrI GCNGC 5 cut(s) 123, 265, 315, 318, 542
Psp124BI GAGCTC 1 cut(s) 81
PsuI RGATCY 1 cut(s) 131
PteI GCGCGC 1 cut(s) 445
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 3 cut(s) 63, 545, 591
SatI GCNGC 5 cut(s) 122, 264, 314, 317, 541
Sau3AI GATC 4 cut(s) 131, 202, 354, 438
SduI GDGCHC 2 cut(s) 81, 331
SetI ASST 9 cut(s) 81, 86, 123, 216, 225, 244, 251, 369, 538
SmlI CTYRAG 5 cut(s) 143, 158, 237, 428, 544
SmoI CTYRAG 5 cut(s) 143, 158, 237, 428, 544
Sse9I AATT 5 cut(s) 42, 227, 571, 576, 619
SsiI CCGC 4 cut(s) 311, 317, 339, 541
SspMI CTAG 1 cut(s) 129
SstI GAGCTC 1 cut(s) 81
StyI CCWWGG 1 cut(s) 367
TaaI ACNGT 2 cut(s) 23, 382
TaiI ACGT 1 cut(s) 86
TaqI TCGA 1 cut(s) 532
TasI AATT 5 cut(s) 42, 227, 571, 576, 619
TauI GCSGC 2 cut(s) 319, 543
Tru1I TTAA 3 cut(s) 63, 545, 591
Tru9I TTAA 3 cut(s) 63, 545, 591
TseI GCWGC 3 cut(s) 121, 263, 313
Van91I CCANNNNNTGG 1 cut(s) 170
Vha464I CTTAAG 1 cut(s) 544
XapI RAATTY 1 cut(s) 227
XbaI TCTAGA 1 cut(s) 128
XspI CTAG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.