Rorug04G0074400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010620
Physical Location & Seq
Forward (+)
11525628 .. 11526652
1025 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0074400.1

Sequence Viewer

Length: 657 bp
ATGGGTCGGATCCAAACCACACCATCTTCCTCTTCTTTATCATCCGACCCACACACCTACCTTGCAGAGGCTACAAATCCATCTTCTCCGTCGCCAATTTCCGGTGTTCTGGAGCAAATGGAGGTTCTCCATTCCAAGCTCGCCGTCACCACCCTCATGGAAGAGCTCCAGAAGCCTCGCCAGTCCCTTTACGTCGCCGTCGCCGAGGAGGGCAACCTCACCACTCAGGTCGACTTTGCTTCACCGGGCCGGAATCGCGATGAGAAACTCAAACGCGACGCCGTCGTCCCCAAGGGCGTCAACCTTGCAGTCTTGGAGGCGTTAGAGAAGGGAGCCACGCCGGAAACACTCGACCCTCGAACCGTAAAAAGGCTGGTCCTGGCCTTGGAGCCCGCTCAATCCTTCACTTCGCAACCGCAGCAAGCGTTAAATCAACACCACATGGACCGGTTCGTGACTTCTCCGCCGCTAGCACAGTTCACTGCCCGCCCGCCTCCTCTGCTGGACGACGATGATTGTAATGGTATGGGTTTTGTGTTTGATGCTGATGGTATTGATGAGAAATTGGAGAATGGGGAGAGTGTCGAGAAGAGGACTAAGGTGAATAGTGGAGATGGTAGAGAGTTCAGTGAGCCTGAGCGTGTCATTGGTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

23.78

Weight (kDa)

4.94

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AccBSI CCGCTC 1 cut(s) 395
AccI GTMKAC 1 cut(s) 231
AccII CGCG 2 cut(s) 258, 276
AciI CCGC 6 cut(s) 393, 416, 464, 467, 487, 491
AclWI GGATC 2 cut(s) 4, 17
AcyI GRCGYC 2 cut(s) 279, 297
AfiI CCNNNNNNNGG 4 cut(s) 67, 101, 369, 385
AgeI ACCGGT 1 cut(s) 447
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 2 cut(s) 139, 166
AluI AGCT 2 cut(s) 139, 166
Alw21I GWGCWC 1 cut(s) 168
AlwI GGATC 2 cut(s) 4, 17
AoxI GGCC 2 cut(s) 247, 381
ApeKI GCWGC 1 cut(s) 418
AsiGI ACCGGT 1 cut(s) 447
AspS9I GGNCC 3 cut(s) 247, 376, 445
AsuC2I CCSGG 1 cut(s) 246
AsuHPI GGTGA 4 cut(s) 139, 211, 234, 613
AsuNHI GCTAGC 1 cut(s) 469
AvaII GGWCC 2 cut(s) 376, 445
BamHI GGATCC 1 cut(s) 9
BanII GRGCYC 2 cut(s) 168, 393
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 1 cut(s) 430
BccI CCATC 4 cut(s) 31, 88, 542, 608
BceAI ACGGC 3 cut(s) 128, 182, 266
BciT130I CCWGG 1 cut(s) 380
BcnI CCSGG 1 cut(s) 246
BfaI CTAG 1 cut(s) 470
BisI GCNGC 2 cut(s) 419, 467
BlsI GCNGC 2 cut(s) 420, 468
Bme1390I CCNGG 2 cut(s) 246, 380
Bme18I GGWCC 2 cut(s) 376, 445
BmgT120I GGNCC 3 cut(s) 247, 376, 445
BmiI GGNNCC 3 cut(s) 11, 334, 390
BmrFI CCNGG 2 cut(s) 246, 380
BmsI GCATC 1 cut(s) 532
BmtI GCTAGC 1 cut(s) 473
BpmI CTGGAG 2 cut(s) 131, 152
Bpu10I CCTNAGC 1 cut(s) 636
BpuMI CCSGG 1 cut(s) 246
BsaHI GRCGYC 2 cut(s) 279, 297
BsaJI CCNNGG 3 cut(s) 204, 291, 384
BsaWI WCCGGW 2 cut(s) 101, 447
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 101, 369, 385
Bse118I RCCGGY 1 cut(s) 447
Bse1I ACTGG 1 cut(s) 181
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 3 cut(s) 204, 291, 384
BseGI GGATG 1 cut(s) 41
BseLI CCNNNNNNNGG 4 cut(s) 67, 101, 369, 385
BseMII CTCAG 2 cut(s) 239, 627
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 2 cut(s) 221, 486
BseXI GCAGC 1 cut(s) 430
Bsh1236I CGCG 2 cut(s) 258, 276
BshFI GGCC 2 cut(s) 249, 383
BshTI ACCGGT 1 cut(s) 447
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 5 cut(s) 102, 245, 250, 341, 448
BslFI GGGAC 2 cut(s) 169, 272
BslI CCNNNNNNNGG 4 cut(s) 67, 101, 369, 385
BsmFI GGGAC 2 cut(s) 169, 272
BsnI GGCC 2 cut(s) 249, 383
Bsp1286I GDGCHC 2 cut(s) 168, 393
Bsp143I GATC 1 cut(s) 9
Bsp68I TCGCGA 1 cut(s) 258
BspACI CCGC 6 cut(s) 393, 416, 464, 467, 487, 491
BspANI GGCC 2 cut(s) 249, 383
BspCNI CTCAG 2 cut(s) 238, 628
BspFNI CGCG 2 cut(s) 258, 276
BspLI GGNNCC 3 cut(s) 11, 334, 390
BspOI GCTAGC 1 cut(s) 473
BspPI GGATC 2 cut(s) 4, 17
BspQI GCTCTTC 1 cut(s) 156
BsrBI CCGCTC 1 cut(s) 395
BsrFI RCCGGY 1 cut(s) 447
BsrI ACTGG 1 cut(s) 181
BssAI RCCGGY 1 cut(s) 447
BssECI CCNNGG 3 cut(s) 204, 291, 384
BssMI GATC 1 cut(s) 9
BssNI GRCGYC 2 cut(s) 279, 297
BssT1I CCWWGG 2 cut(s) 291, 384
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 2 cut(s) 364, 477
Bst6I CTCTTC 3 cut(s) 37, 156, 584
BstACI GRCGYC 2 cut(s) 279, 297
BstC8I GCNNGC 6 cut(s) 141, 393, 423, 471, 487, 491
BstDEI CTNAG 3 cut(s) 225, 597, 636
BstENI CCTNNNNNAGG 1 cut(s) 65
BstF5I GGATG 1 cut(s) 41
BstFNI CGCG 2 cut(s) 258, 276
BstKTI GATC 1 cut(s) 12
BstMBI GATC 1 cut(s) 9
BstMWI GCNNNNNNNGC 4 cut(s) 172, 255, 418, 499
BstNI CCWGG 1 cut(s) 380
BstSCI CCNGG 2 cut(s) 244, 378
BstUI CGCG 2 cut(s) 258, 276
BstV1I GCAGC 1 cut(s) 430
BstX2I RGATCY 1 cut(s) 9
BstXI CCANNNNNNTGG 1 cut(s) 157
BstYI RGATCY 1 cut(s) 9
BsuRI GGCC 2 cut(s) 249, 383
BtgZI GCGATG 1 cut(s) 273
BtsCI GGATG 1 cut(s) 41
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 2 cut(s) 480, 634
BtuMI TCGCGA 1 cut(s) 258
Cac8I GCNNGC 6 cut(s) 141, 393, 423, 471, 487, 491
Cfr10I RCCGGY 1 cut(s) 447
Cfr13I GGNCC 3 cut(s) 247, 376, 445
CseI GACGC 2 cut(s) 286, 287
CspAI ACCGGT 1 cut(s) 447
CviAII CATG 2 cut(s) 157, 442
DdeI CTNAG 3 cut(s) 225, 597, 636
DpnI GATC 1 cut(s) 11
DpnII GATC 1 cut(s) 9
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
Eam1104I CTCTTC 3 cut(s) 37, 156, 584
EarI CTCTTC 3 cut(s) 37, 156, 584
EciI GGCGGA 1 cut(s) 453
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 2 cut(s) 291, 384
Eco24I GRGCYC 2 cut(s) 168, 393
Eco47I GGWCC 2 cut(s) 376, 445
Eco53kI GAGCTC 1 cut(s) 166
EcoICRI GAGCTC 1 cut(s) 166
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoRII CCWGG 1 cut(s) 378
EcoT14I CCWWGG 2 cut(s) 291, 384
EcoT38I GRGCYC 2 cut(s) 168, 393
ErhI CCWWGG 2 cut(s) 291, 384
FaeI CATG 2 cut(s) 160, 445
FaiI YATR 3 cut(s) 158, 443, 527
FaqI GGGAC 2 cut(s) 169, 272
FatI CATG 2 cut(s) 156, 441
FauI CCCGC 3 cut(s) 400, 494, 498
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 2 cut(s) 419, 467
FokI GGATG 1 cut(s) 28
FriOI GRGCYC 2 cut(s) 168, 393
Fsp4HI GCNGC 2 cut(s) 419, 467
FspBI CTAG 1 cut(s) 470
GluI GCNGC 2 cut(s) 419, 467
GsuI CTGGAG 2 cut(s) 131, 152
HaeIII GGCC 2 cut(s) 249, 383
HapII CCGG 5 cut(s) 102, 245, 250, 341, 448
HgaI GACGC 2 cut(s) 286, 287
Hin1I GRCGYC 2 cut(s) 279, 297
Hin1II CATG 2 cut(s) 160, 445
HincII GTYRAC 2 cut(s) 232, 301
HindII GTYRAC 2 cut(s) 232, 301
HinfI GANTC 1 cut(s) 253
HpaII CCGG 5 cut(s) 102, 245, 250, 341, 448
HphI GGTGA 4 cut(s) 139, 211, 234, 613
Hpy166II GTNNAC 3 cut(s) 232, 301, 480
Hpy188I TCNGA 2 cut(s) 9, 46
Hpy188III TCNNGA 5 cut(s) 110, 169, 257, 454, 586
Hpy8I GTNNAC 3 cut(s) 232, 301, 480
Hpy99I CGWCG 6 cut(s) 94, 197, 203, 281, 287, 512
HpyAV CCTTC 2 cut(s) 322, 412
HpyCH4III ACNGT 2 cut(s) 364, 477
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 2 cut(s) 65, 308
HpyF10VI GCNNNNNNNGC 4 cut(s) 172, 255, 418, 499
HpyF3I CTNAG 3 cut(s) 225, 597, 636
HpySE526I ACGT 1 cut(s) 192
Hsp92I GRCGYC 2 cut(s) 279, 297
Hsp92II CATG 2 cut(s) 160, 445
Kzo9I GATC 1 cut(s) 9
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 4 cut(s) 112, 171, 332, 388
Lsp1109I GCAGC 1 cut(s) 430
LweI GCATC 1 cut(s) 532
MaeI CTAG 1 cut(s) 470
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 2 cut(s) 145, 454
MalI GATC 1 cut(s) 11
MbiI CCGCTC 1 cut(s) 395
MboI GATC 1 cut(s) 9
MboII GAAGA 5 cut(s) 18, 24, 75, 173, 601
MflI RGATCY 1 cut(s) 9
MhlI GDGCHC 2 cut(s) 168, 393
MluCI AATT 2 cut(s) 96, 563
MmeI TCCRAC 1 cut(s) 69
MseI TTAA 1 cut(s) 428
MslI CAYNNNNRTG 1 cut(s) 155
MspI CCGG 5 cut(s) 102, 245, 250, 341, 448
MspR9I CCNGG 2 cut(s) 246, 380
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 2 cut(s) 258, 276
MwoI GCNNNNNNNGC 4 cut(s) 172, 255, 418, 499
NciI CCSGG 1 cut(s) 246
NdeII GATC 1 cut(s) 9
NheI GCTAGC 1 cut(s) 469
NlaIII CATG 2 cut(s) 160, 445
NlaIV GGNNCC 3 cut(s) 11, 334, 390
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 2 cut(s) 145, 454
NruI TCGCGA 1 cut(s) 258
PciSI GCTCTTC 1 cut(s) 156
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PfeI GAWTC 1 cut(s) 253
PflFI GACNNNGTC 1 cut(s) 281
PinAI ACCGGT 1 cut(s) 447
PkrI GCNGC 2 cut(s) 420, 468
Psp124BI GAGCTC 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 378
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 3 cut(s) 11, 334, 390
PspPI GGNCC 3 cut(s) 247, 376, 445
PsuI RGATCY 1 cut(s) 9
PsyI GACNNNGTC 1 cut(s) 281
RruI TCGCGA 1 cut(s) 258
RseI CAYNNNNRTG 1 cut(s) 155
SacI GAGCTC 1 cut(s) 168
SalI GTCGAC 1 cut(s) 230
SapI GCTCTTC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 428
SatI GCNGC 2 cut(s) 419, 467
Sau3AI GATC 1 cut(s) 9
Sau96I GGNCC 3 cut(s) 247, 376, 445
ScrFI CCNGG 2 cut(s) 246, 380
SduI GDGCHC 2 cut(s) 168, 393
SfaNI GCATC 1 cut(s) 532
SinI GGWCC 2 cut(s) 376, 445
SmiMI CAYNNNNRTG 1 cut(s) 155
Sse9I AATT 2 cut(s) 96, 563
SsiI CCGC 6 cut(s) 393, 416, 464, 467, 487, 491
SspMI CTAG 1 cut(s) 470
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 244, 378
StyI CCWWGG 2 cut(s) 291, 384
TaaI ACNGT 2 cut(s) 364, 477
TaiI ACGT 1 cut(s) 195
TaqI TCGA 4 cut(s) 231, 351, 358, 585
TasI AATT 2 cut(s) 96, 563
TauI GCSGC 1 cut(s) 469
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 1 cut(s) 428
Tru9I TTAA 1 cut(s) 428
TscAI CASTG 2 cut(s) 487, 634
TseFI GTSAC 2 cut(s) 145, 454
TseI GCWGC 1 cut(s) 418
Tsp45I GTSAC 2 cut(s) 145, 454
TspGWI ACGGA 1 cut(s) 78
TspRI CASTG 2 cut(s) 487, 634
Tth111I GACNNNGTC 1 cut(s) 281
VpaK11BI GGWCC 2 cut(s) 376, 445
XagI CCTNNNNNAGG 1 cut(s) 65
XmiI GTMKAC 1 cut(s) 231
XspI CTAG 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.