Rroxscaffold_4G00301550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010620
Physical Location & Seq
Forward (+)
21868794 .. 21879609
10816 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00301550.1

Sequence Viewer

Length: 687 bp
ATGCCACGGTGGTTTGAGCTCAACCTTGATCGCCGCAAAGCGATTTCGCCGAAGGAGGCCGTCGGCGCCATGGCTCAAGTAGCCTTAACCGACATAGAGATCCCCAAAAGACCGACCTCGCGTCACTTCGAAGATCGTTGCACTGATCCCGATGAACCGGGTGATCATGTGATTTTCTATGAGCGTGATCATGTGATTCGTGTGACCAACCTAGGGAAAACCCGCATCCTTAGAGTTACTCTTACTAATCTTATAAGATCTCAACCATTTCCTCTATTAATGCTCCATCAAACTTACCACAATTCAAACCCTATGATTTCACTATTCGAATCCGAATCAAACTCCATATCACATATTTTCGGTTCAACCGGTTCGAGTTCCGGCCGCTTCCGGAGAGGCCGCCGCCGTGCAAGGGAGGTTCGGGAGCTTCGGAAGGTCAAGGCGAACCTCGTGGGTGATGTTTGGAAAGGTTTCGTGGCCGTAGCTCGAGTTTTCAAGCTTGGAACAAAAACGGGTTTTCCGCCTCCGCCGTCGTCTCCGTGGTTCTGCGGCGCAAGGCCGCCTCCCGCACTGCGCACGATCGTCTTGGAGGTGTGGGAGTTGATGTGGTGCCGCCCGGTGGCCGGTGGAGCGAGAGAGAATGGTGAGAGCAATTGCTCGTTTTTCGGACGGGGAAGAAGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

228

Amino Acids

25.9

Weight (kDa)

10.35

Isoelectric Point (pI)

58.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
Acc16I TGCGCA 1 cut(s) 575
AccB1I GGYRCC 2 cut(s) 65, 609
AccII CGCG 1 cut(s) 121
AccIII TCCGGA 1 cut(s) 390
AclWI GGATC 2 cut(s) 94, 140
AcoI YGGCCR 3 cut(s) 382, 477, 621
AcyI GRCGYC 1 cut(s) 66
AfiI CCNNNNNNNGG 4 cut(s) 213, 412, 619, 623
AgeI ACCGGT 1 cut(s) 368
AgsI TTSAA 3 cut(s) 306, 366, 496
AhdI GACNNNNNGTC 1 cut(s) 120
AluBI AGCT 4 cut(s) 19, 427, 485, 499
AluI AGCT 4 cut(s) 19, 427, 485, 499
Alw21I GWGCWC 1 cut(s) 21
Alw26I GTCTC 1 cut(s) 540
AlwI GGATC 2 cut(s) 94, 140
Ama87I CYCGRG 1 cut(s) 486
Aor13HI TCCGGA 1 cut(s) 390
AoxI GGCC 6 cut(s) 57, 382, 397, 477, 557, 621
AseI ATTAAT 1 cut(s) 278
AsiGI ACCGGT 1 cut(s) 368
Asp700I GAANNNNTTC 1 cut(s) 470
AspA2I CCTAGG 1 cut(s) 211
AspLEI GCGC 3 cut(s) 68, 554, 576
AsuC2I CCSGG 2 cut(s) 159, 617
AsuHPI GGTGA 3 cut(s) 173, 467, 656
AsuII TTCGAA 2 cut(s) 129, 327
AvaI CYCGRG 1 cut(s) 486
AvrII CCTAGG 1 cut(s) 211
BanI GGYRCC 2 cut(s) 65, 609
BanII GRGCYC 1 cut(s) 21
BauI CACGAG 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 21
BccI CCATC 1 cut(s) 294
BceAI ACGGC 4 cut(s) 44, 390, 464, 514
BclI TGATCA 2 cut(s) 163, 187
BcnI CCSGG 2 cut(s) 159, 617
BcoDI GTCTC 1 cut(s) 540
BfaI CTAG 1 cut(s) 212
BfoI RGCGCY 1 cut(s) 69
BglII AGATCT 1 cut(s) 257
BisI GCNGC 7 cut(s) 34, 385, 400, 403, 550, 560, 613
BlnI CCTAGG 1 cut(s) 211
BlsI GCNGC 7 cut(s) 35, 386, 401, 404, 551, 561, 614
Bme1390I CCNGG 2 cut(s) 159, 617
BmeRI GACNNNNNGTC 1 cut(s) 120
BmeT110I CYCGRG 1 cut(s) 486
BmiI GGNNCC 2 cut(s) 67, 611
BmrFI CCNGG 2 cut(s) 159, 617
BmsI GCATC 1 cut(s) 234
Bpu14I TTCGAA 2 cut(s) 129, 327
BpuEI CTTGAG 1 cut(s) 60
BpuMI CCSGG 2 cut(s) 159, 617
BsaHI GRCGYC 1 cut(s) 66
BsaJI CCNNGG 4 cut(s) 5, 69, 211, 539
BsaWI WCCGGW 2 cut(s) 368, 390
BsaXI ACNNNNNCTCC 2 cut(s) 590, 620
Bsc4I CCNNNNNNNGG 4 cut(s) 213, 412, 619, 623
Bse118I RCCGGY 2 cut(s) 368, 623
BseAI TCCGGA 1 cut(s) 390
BseDI CCNNGG 4 cut(s) 5, 69, 211, 539
BseGI GGATG 1 cut(s) 225
BseLI CCNNNNNNNGG 4 cut(s) 213, 412, 619, 623
BseX3I CGGCCG 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 121
Bsh1285I CGRYCG 2 cut(s) 385, 582
BshFI GGCC 6 cut(s) 59, 384, 399, 479, 559, 623
BshNI GGYRCC 2 cut(s) 65, 609
BshTI ACCGGT 1 cut(s) 368
BsiEI CGRYCG 2 cut(s) 385, 582
BsiHKAI GWGCWC 1 cut(s) 21
BsiHKCI CYCGRG 1 cut(s) 486
BsiSI CCGG 6 cut(s) 158, 369, 381, 391, 617, 624
BslI CCNNNNNNNGG 4 cut(s) 213, 412, 619, 623
BsmAI GTCTC 1 cut(s) 540
BsmBI CGTCTC 1 cut(s) 540
BsnI GGCC 6 cut(s) 59, 384, 399, 479, 559, 623
BsoBI CYCGRG 1 cut(s) 486
Bsp119I TTCGAA 2 cut(s) 129, 327
Bsp1286I GDGCHC 1 cut(s) 21
Bsp13I TCCGGA 1 cut(s) 390
Bsp143I GATC 8 cut(s) 28, 99, 133, 145, 163, 187, 257, 579
Bsp19I CCATGG 1 cut(s) 69
BspANI GGCC 6 cut(s) 59, 384, 399, 479, 559, 623
BspEI TCCGGA 1 cut(s) 390
BspFNI CGCG 1 cut(s) 121
BspLI GGNNCC 2 cut(s) 67, 611
BspPI GGATC 2 cut(s) 94, 140
BspT104I TTCGAA 2 cut(s) 129, 327
BspT107I GGYRCC 2 cut(s) 65, 609
BsrFI RCCGGY 2 cut(s) 368, 623
BssAI RCCGGY 2 cut(s) 368, 623
BssECI CCNNGG 4 cut(s) 5, 69, 211, 539
BssMI GATC 8 cut(s) 28, 99, 133, 145, 163, 187, 257, 579
BssNI GRCGYC 1 cut(s) 66
BssSI CACGAG 1 cut(s) 449
BssT1I CCWWGG 2 cut(s) 69, 211
Bst2BI CACGAG 1 cut(s) 449
Bst4CI ACNGT 1 cut(s) 9
BstACI GRCGYC 1 cut(s) 66
BstBI TTCGAA 2 cut(s) 129, 327
BstDEI CTNAG 1 cut(s) 230
BstDSI CCRYGG 3 cut(s) 5, 69, 539
BstF5I GGATG 1 cut(s) 225
BstFNI CGCG 1 cut(s) 121
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 3 cut(s) 68, 554, 576
BstKTI GATC 8 cut(s) 31, 102, 136, 148, 166, 190, 260, 582
BstMAI GTCTC 1 cut(s) 540
BstMBI GATC 8 cut(s) 28, 99, 133, 145, 163, 187, 257, 579
BstMCI CGRYCG 2 cut(s) 385, 582
BstMWI GCNNNNNNNGC 3 cut(s) 65, 80, 629
BstSCI CCNGG 2 cut(s) 157, 615
BstUI CGCG 1 cut(s) 121
BstX2I RGATCY 2 cut(s) 99, 257
BstYI RGATCY 2 cut(s) 99, 257
BstZI CGGCCG 1 cut(s) 382
BsuRI GGCC 6 cut(s) 59, 384, 399, 479, 559, 623
BtgI CCRYGG 3 cut(s) 5, 69, 539
BtsCI GGATG 1 cut(s) 225
BtsI GCAGTG 1 cut(s) 569
BtsIMutI CAGTG 2 cut(s) 141, 569
CfoI GCGC 3 cut(s) 68, 554, 576
Cfr10I RCCGGY 2 cut(s) 368, 623
CseI GACGC 1 cut(s) 110
CspAI ACCGGT 1 cut(s) 368
CviAII CATG 3 cut(s) 70, 167, 191
DdeI CTNAG 1 cut(s) 230
DinI GGCGCC 1 cut(s) 67
DpnI GATC 8 cut(s) 30, 101, 135, 147, 165, 189, 259, 581
DpnII GATC 8 cut(s) 28, 99, 133, 145, 163, 187, 257, 579
DriI GACNNNNNGTC 1 cut(s) 120
EaeI YGGCCR 3 cut(s) 382, 477, 621
EagI CGGCCG 1 cut(s) 382
Eam1105I GACNNNNNGTC 1 cut(s) 120
EciI GGCGGA 2 cut(s) 510, 516
Ecl136II GAGCTC 1 cut(s) 19
EclXI CGGCCG 1 cut(s) 382
Eco130I CCWWGG 2 cut(s) 69, 211
Eco24I GRGCYC 1 cut(s) 21
Eco52I CGGCCG 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 19
Eco88I CYCGRG 1 cut(s) 486
EcoICRI GAGCTC 1 cut(s) 19
EcoT14I CCWWGG 2 cut(s) 69, 211
EcoT38I GRGCYC 1 cut(s) 21
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
ErhI CCWWGG 2 cut(s) 69, 211
Esp3I CGTCTC 1 cut(s) 540
FaeI CATG 3 cut(s) 73, 170, 194
FaiI YATR 9 cut(s) 71, 95, 168, 180, 192, 254, 314, 347, 354
FatI CATG 3 cut(s) 69, 166, 190
FauI CCCGC 2 cut(s) 230, 574
FbaI TGATCA 2 cut(s) 163, 187
Fnu4HI GCNGC 7 cut(s) 34, 385, 400, 403, 550, 560, 613
FokI GGATG 1 cut(s) 212
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 7 cut(s) 34, 385, 400, 403, 550, 560, 613
FspBI CTAG 1 cut(s) 212
FspI TGCGCA 1 cut(s) 575
GlaI GCGC 3 cut(s) 67, 553, 575
GluI GCNGC 7 cut(s) 34, 385, 400, 403, 550, 560, 613
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 6 cut(s) 59, 384, 399, 479, 559, 623
HapII CCGG 6 cut(s) 158, 369, 381, 391, 617, 624
HgaI GACGC 1 cut(s) 110
HhaI GCGC 3 cut(s) 68, 554, 576
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 3 cut(s) 73, 170, 194
Hin6I GCGC 3 cut(s) 66, 552, 574
HinP1I GCGC 3 cut(s) 66, 552, 574
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 3 cut(s) 196, 329, 335
HpaII CCGG 6 cut(s) 158, 369, 381, 391, 617, 624
HphI GGTGA 3 cut(s) 173, 467, 656
Hpy188I TCNGA 3 cut(s) 334, 432, 668
Hpy188III TCNNGA 3 cut(s) 149, 391, 422
Hpy99I CGWCG 2 cut(s) 65, 535
HpyAV CCTTC 2 cut(s) 46, 427
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4V TGCA 2 cut(s) 141, 410
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 80, 629
HpyF3I CTNAG 1 cut(s) 230
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 3 cut(s) 73, 170, 194
HspAI GCGC 3 cut(s) 66, 552, 574
KasI GGCGCC 1 cut(s) 65
Kpn2I TCCGGA 1 cut(s) 390
Ksp22I TGATCA 2 cut(s) 163, 187
Kzo9I GATC 8 cut(s) 28, 99, 133, 145, 163, 187, 257, 579
LmnI GCTCC 3 cut(s) 288, 424, 629
LpnPI CCDG 6 cut(s) 171, 382, 394, 404, 630, 637
LweI GCATC 1 cut(s) 234
MaeI CTAG 1 cut(s) 212
MaeIII GTNAC 3 cut(s) 122, 202, 235
MalI GATC 8 cut(s) 30, 101, 135, 147, 165, 189, 259, 581
MboI GATC 8 cut(s) 28, 99, 133, 145, 163, 187, 257, 579
MboII GAAGA 2 cut(s) 143, 687
MfeI CAATTG 1 cut(s) 652
MflI RGATCY 2 cut(s) 99, 257
MhlI GDGCHC 1 cut(s) 21
MluCI AATT 2 cut(s) 301, 652
Mly113I GGCGCC 1 cut(s) 66
MnlI CCTC 9 cut(s) 49, 127, 282, 389, 409, 458, 534, 573, 583
MroI TCCGGA 1 cut(s) 390
MroXI GAANNNNTTC 1 cut(s) 470
MseI TTAA 2 cut(s) 86, 278
MspI CCGG 6 cut(s) 158, 369, 381, 391, 617, 624
MspR9I CCNGG 2 cut(s) 159, 617
MunI CAATTG 1 cut(s) 652
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 3 cut(s) 65, 80, 629
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 2 cut(s) 159, 617
NcoI CCATGG 1 cut(s) 69
NdeII GATC 8 cut(s) 28, 99, 133, 145, 163, 187, 257, 579
NlaIII CATG 3 cut(s) 73, 170, 194
NlaIV GGNNCC 2 cut(s) 67, 611
NmuCI GTSAC 2 cut(s) 122, 202
NsbI TGCGCA 1 cut(s) 575
NspV TTCGAA 2 cut(s) 129, 327
PaeR7I CTCGAG 1 cut(s) 486
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 3 cut(s) 196, 329, 335
PinAI ACCGGT 1 cut(s) 368
PkrI GCNGC 7 cut(s) 35, 386, 401, 404, 551, 561, 614
Ple19I CGATCG 1 cut(s) 582
PluTI GGCGCC 1 cut(s) 69
PshBI ATTAAT 1 cut(s) 278
PsiI TTATAA 1 cut(s) 254
Psp124BI GAGCTC 1 cut(s) 21
PspN4I GGNNCC 2 cut(s) 67, 611
PspXI VCTCGAGB 1 cut(s) 486
PsuI RGATCY 2 cut(s) 99, 257
PvuI CGATCG 1 cut(s) 582
SacI GAGCTC 1 cut(s) 21
SaqAI TTAA 2 cut(s) 86, 278
SatI GCNGC 7 cut(s) 34, 385, 400, 403, 550, 560, 613
Sau3AI GATC 8 cut(s) 28, 99, 133, 145, 163, 187, 257, 579
ScrFI CCNGG 2 cut(s) 159, 617
SduI GDGCHC 1 cut(s) 21
SfaNI GCATC 1 cut(s) 234
SfoI GGCGCC 1 cut(s) 67
Sfr274I CTCGAG 1 cut(s) 486
SfuI TTCGAA 2 cut(s) 129, 327
SlaI CTCGAG 1 cut(s) 486
SmlI CTYRAG 2 cut(s) 75, 486
SmoI CTYRAG 2 cut(s) 75, 486
Sse9I AATT 2 cut(s) 301, 652
SspDI GGCGCC 1 cut(s) 65
SspMI CTAG 1 cut(s) 212
SstI GAGCTC 1 cut(s) 21
StyD4I CCNGG 2 cut(s) 157, 615
StyI CCWWGG 2 cut(s) 69, 211
TaaI ACNGT 1 cut(s) 9
TaqI TCGA 4 cut(s) 129, 327, 374, 487
TaqII GACCGA 1 cut(s) 127
TasI AATT 2 cut(s) 301, 652
TauI GCSGC 7 cut(s) 36, 387, 402, 405, 552, 562, 615
TfiI GAWTC 3 cut(s) 196, 329, 335
Tru1I TTAA 2 cut(s) 86, 278
Tru9I TTAA 2 cut(s) 86, 278
TscAI CASTG 2 cut(s) 148, 576
TseFI GTSAC 2 cut(s) 122, 202
Tsp45I GTSAC 2 cut(s) 122, 202
TspDTI ATGAA 1 cut(s) 168
TspGWI ACGGA 1 cut(s) 528
TspRI CASTG 2 cut(s) 148, 576
VspI ATTAAT 1 cut(s) 278
XhoI CTCGAG 1 cut(s) 486
XmaJI CCTAGG 1 cut(s) 211
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.