Rmu_ssc0000196.1_g000019

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000196.1
OG0002066
Physical Location & Seq
Forward (+)
96439 .. 99714
3276 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1215 bp
ctgcctaattgtacagctgaccctgggataaggctggtggggttattggttggaggaagtattgcatccattttggatgtggaggcatttgaggctggcccaggttccgatgcagctggcaaacaagaaggattactttggtataaagactttgggcaacatttgaatggtgacttttcaatggctgttgtacaggccaacaatttgcttgaggatcagagccagcttgagtctggtcccttgagctcgcttgagtctggcccctatggcacttttattggagtttatgatgggcacggcggccctgagacctcccgttatgtcaatgatcacctattccagaatttgaaaaggttcacgtcagagcaacagtccatgtcagtggatgtgatacggaaagcatatcaagcaacagaagatggattcctctctattgttaccaaacaatggcctgtgaagccccaaattgcagccgttggatcttgttgccttgttggtgtgatctgtggaggcacactgtacattgccaacgttggtgactcccgcgctgtgctggggagagttgtgaaggcaactggagaggtacttgccatccagctgtcagcagagcacaatgtagcgatagagtctgtcagacgggagatgcattctttgcatccagacgactctcatattgtagttttgaagcataatgtatggcgtgtaaagggattgatacagatttctagatccattggagatgtatatctaaaaaagcccgagtttaacagggagcctttgtatgccaagtttcgcctgcgtgaacctttcaaaaggccaattttaagcgctgaacctgcaatttctgtgcatgaacttcaaccacatgatcaatttctcatatttgcttcggatggactctgggagcatcttaccaatcaggaagcagttgatatagttcaaagtcacccacacagcggaagtgctcggaggcttgtcaaagctgcattgctggaagcggctaagaagagagaaatgaggtactccgatttgaagaaaatagaccgtggtgtccgcaggcatttccacgatgacataacagtcatagttgtatttctggactcgaaccttgtgagcagagctagctcaaccaggggccctactttgtctgtgagaggaggtggtattaatctacccgcaaaaactcttgcccccatggaactgaatgctacctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

404

Amino Acids

44.24

Weight (kDa)

6.78

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 90 - 336 1.3e-39 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 1049, 1079
Acc36I ACCTGC 1 cut(s) 844
AccB7I CCANNNNNTGG 1 cut(s) 447
AccII CGCG 1 cut(s) 546
AciI CCGC 6 cut(s) 300, 544, 957, 998, 1054, 1176
AclI AACGTT 1 cut(s) 531
AclWI GGATC 3 cut(s) 222, 487, 723
AcsI RAATTY 1 cut(s) 343
AfaI GTAC 5 cut(s) 13, 192, 521, 585, 1022
AfeI AGCGCT 1 cut(s) 829
AfiI CCNNNNNNNGG 2 cut(s) 447, 956
AgsI TTSAA 8 cut(s) 166, 180, 349, 685, 811, 860, 941, 1033
AjiI CACGTC 1 cut(s) 360
AjnI CCWGG 3 cut(s) 22, 100, 1130
AluBI AGCT 8 cut(s) 17, 116, 226, 246, 598, 983, 1121, 1125
AluI AGCT 8 cut(s) 17, 116, 226, 246, 598, 983, 1121, 1125
Alw21I GWGCWC 3 cut(s) 248, 612, 967
Alw26I GTCTC 1 cut(s) 302
AlwI GGATC 3 cut(s) 222, 487, 723
Ama87I CYCGRG 1 cut(s) 758
Aor51HI AGCGCT 1 cut(s) 829
AoxI GGCC 7 cut(s) 97, 195, 259, 301, 449, 815, 1135
ApaI GGGCCC 1 cut(s) 1139
ApeKI GCWGC 3 cut(s) 113, 470, 983
ApoI RAATTY 1 cut(s) 343
AseI ATTAAT 1 cut(s) 1167
Asp700I GAANNNNTTC 1 cut(s) 353
AspLEI GCGC 2 cut(s) 548, 830
AspS9I GGNCC 6 cut(s) 98, 236, 260, 302, 1135, 1136
AsuHPI GGTGA 4 cut(s) 182, 323, 548, 938
AsuNHI GCTAGC 1 cut(s) 1121
AvaI CYCGRG 1 cut(s) 758
AvaII GGWCC 1 cut(s) 236
BaeGI GKGCMC 2 cut(s) 297, 1139
BanII GRGCYC 2 cut(s) 248, 1139
Bbv12I GWGCWC 3 cut(s) 248, 612, 967
BbvI GCAGC 3 cut(s) 125, 482, 970
BccI CCATC 4 cut(s) 284, 413, 599, 887
BceAI ACGGC 2 cut(s) 313, 458
BciT130I CCWGG 3 cut(s) 24, 102, 1132
BclI TGATCA 2 cut(s) 328, 868
BcoDI GTCTC 1 cut(s) 302
BfaI CTAG 2 cut(s) 726, 1122
BfoI RGCGCY 1 cut(s) 831
BfuAI ACCTGC 1 cut(s) 844
BglI GCCNNNNNGGC 1 cut(s) 267
BisI GCNGC 5 cut(s) 114, 301, 471, 984, 999
BlsI GCNGC 5 cut(s) 115, 302, 472, 985, 1000
Bme1390I CCNGG 3 cut(s) 24, 102, 1132
Bme18I GGWCC 1 cut(s) 236
BmeT110I CYCGRG 1 cut(s) 758
BmgBI CACGTC 1 cut(s) 360
BmgT120I GGNCC 6 cut(s) 98, 236, 260, 302, 1135, 1136
BmiI GGNNCC 6 cut(s) 106, 238, 262, 774, 1136, 1137
BmrFI CCNGG 3 cut(s) 24, 102, 1132
BmsI GCATC 5 cut(s) 74, 100, 633, 664, 916
BmtI GCTAGC 1 cut(s) 1125
BpmI CTGGAG 1 cut(s) 597
BpuEI CTTGAG 4 cut(s) 230, 248, 262, 272
BsaBI GATNNNNATC 1 cut(s) 744
BsaI GGTCTC 1 cut(s) 302
BsaJI CCNNGG 6 cut(s) 22, 23, 100, 1045, 1131, 1194
BsaXI ACNNNNNCTCC 2 cut(s) 764, 794
Bsc4I CCNNNNNNNGG 2 cut(s) 447, 956
Bse1I ACTGG 1 cut(s) 580
Bse3DI GCAATG 2 cut(s) 522, 986
Bse8I GATNNNNATC 1 cut(s) 744
BseBI CCWGG 3 cut(s) 24, 102, 1132
BseDI CCNNGG 6 cut(s) 22, 23, 100, 1045, 1131, 1194
BseGI GGATG 6 cut(s) 65, 82, 391, 591, 655, 898
BseJI GATNNNNATC 1 cut(s) 744
BseLI CCNNNNNNNGG 2 cut(s) 447, 956
BseMI GCAATG 2 cut(s) 522, 986
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 1 cut(s) 1170
BseSI GKGCMC 2 cut(s) 297, 1139
BseXI GCAGC 3 cut(s) 125, 482, 970
BseYI CCCAGC 1 cut(s) 553
Bsh1236I CGCG 1 cut(s) 546
BshFI GGCC 7 cut(s) 99, 197, 261, 303, 451, 817, 1137
BsiHKAI GWGCWC 3 cut(s) 248, 612, 967
BsiHKCI CYCGRG 1 cut(s) 758
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 2 cut(s) 447, 956
BsmAI GTCTC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 2 cut(s) 646, 1210
BsnI GGCC 7 cut(s) 99, 197, 261, 303, 451, 817, 1137
Bso31I GGTCTC 1 cut(s) 302
BsoBI CYCGRG 1 cut(s) 758
Bsp120I GGGCCC 1 cut(s) 1135
Bsp1286I GDGCHC 5 cut(s) 248, 297, 612, 967, 1139
Bsp1407I TGTACA 3 cut(s) 11, 190, 519
Bsp143I GATC 6 cut(s) 214, 328, 479, 501, 728, 868
Bsp19I CCATGG 1 cut(s) 1194
BspACI CCGC 6 cut(s) 300, 544, 957, 998, 1054, 1176
BspANI GGCC 7 cut(s) 99, 197, 261, 303, 451, 817, 1137
BspCNI CTCAG 1 cut(s) 298
BspFNI CGCG 1 cut(s) 546
BspLI GGNNCC 6 cut(s) 106, 238, 262, 774, 1136, 1137
BspMI ACCTGC 1 cut(s) 844
BspOI GCTAGC 1 cut(s) 1125
BspPI GGATC 3 cut(s) 222, 487, 723
BspTNI GGTCTC 1 cut(s) 302
BsrDI GCAATG 2 cut(s) 522, 986
BsrGI TGTACA 3 cut(s) 11, 190, 519
BsrI ACTGG 1 cut(s) 580
BssECI CCNNGG 6 cut(s) 22, 23, 100, 1045, 1131, 1194
BssMI GATC 6 cut(s) 214, 328, 479, 501, 728, 868
BssT1I CCWWGG 1 cut(s) 1194
Bst2UI CCWGG 3 cut(s) 24, 102, 1132
Bst4CI ACNGT 4 cut(s) 372, 519, 1046, 1081
Bst6I CTCTTC 1 cut(s) 1001
BstAPI GCANNNNNTGC 1 cut(s) 652
BstAUI TGTACA 3 cut(s) 11, 190, 519
BstC8I GCNNGC 7 cut(s) 97, 118, 224, 248, 797, 1058, 1123
BstDEI CTNAG 2 cut(s) 306, 1002
BstDSI CCRYGG 2 cut(s) 1045, 1194
BstF5I GGATG 6 cut(s) 65, 82, 391, 591, 655, 898
BstFNI CGCG 1 cut(s) 546
BstH2I RGCGCY 1 cut(s) 831
BstHHI GCGC 2 cut(s) 548, 830
BstKTI GATC 6 cut(s) 217, 331, 482, 504, 731, 871
BstMAI GTCTC 1 cut(s) 302
BstMBI GATC 6 cut(s) 214, 328, 479, 501, 728, 868
BstMWI GCNNNNNNNGC 7 cut(s) 92, 267, 407, 457, 652, 836, 1122
BstNI CCWGG 3 cut(s) 24, 102, 1132
BstSCI CCNGG 3 cut(s) 22, 100, 1130
BstSLI GKGCMC 2 cut(s) 297, 1139
BstUI CGCG 1 cut(s) 546
BstV1I GCAGC 3 cut(s) 125, 482, 970
BstX2I RGATCY 2 cut(s) 479, 728
BstXI CCANNNNNNTGG 1 cut(s) 382
BstYI RGATCY 2 cut(s) 479, 728
BsuRI GGCC 7 cut(s) 99, 197, 261, 303, 451, 817, 1137
BtgI CCRYGG 2 cut(s) 1045, 1194
BtrI CACGTC 1 cut(s) 360
BtsCI GGATG 6 cut(s) 65, 82, 391, 591, 655, 898
BtsIMutI CAGTG 2 cut(s) 387, 515
BveI ACCTGC 1 cut(s) 844
Cac8I GCNNGC 7 cut(s) 97, 118, 224, 248, 797, 1058, 1123
CfoI GCGC 2 cut(s) 548, 830
Cfr13I GGNCC 6 cut(s) 98, 236, 260, 302, 1135, 1136
Csp6I GTAC 5 cut(s) 12, 191, 520, 584, 1021
CviAII CATG 4 cut(s) 376, 851, 866, 1195
CviQI GTAC 5 cut(s) 12, 191, 520, 584, 1021
DdeI CTNAG 2 cut(s) 306, 1002
DpnI GATC 6 cut(s) 216, 330, 481, 503, 730, 870
DpnII GATC 6 cut(s) 214, 328, 479, 501, 728, 868
DrdI GACNNNNNNGTC 2 cut(s) 1049, 1079
DseDI GACNNNNNNGTC 2 cut(s) 1049, 1079
Eam1104I CTCTTC 1 cut(s) 1001
EarI CTCTTC 1 cut(s) 1001
Ecl136II GAGCTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 1194
Eco24I GRGCYC 2 cut(s) 248, 1139
Eco31I GGTCTC 1 cut(s) 302
Eco47I GGWCC 1 cut(s) 236
Eco47III AGCGCT 1 cut(s) 829
Eco53kI GAGCTC 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 758
EcoICRI GAGCTC 1 cut(s) 246
EcoO109I RGGNCCY 2 cut(s) 1135, 1136
EcoRII CCWGG 3 cut(s) 22, 100, 1130
EcoT14I CCWWGG 1 cut(s) 1194
EcoT22I ATGCAT 1 cut(s) 648
EcoT38I GRGCYC 2 cut(s) 248, 1139
ErhI CCWWGG 1 cut(s) 1194
FaeI CATG 4 cut(s) 379, 854, 869, 1198
FalI AAGNNNNNCTT 2 cut(s) 120, 152
FaqI GGGAC 1 cut(s) 222
FatI CATG 4 cut(s) 375, 850, 865, 1194
FauI CCCGC 2 cut(s) 551, 1183
FbaI TGATCA 2 cut(s) 328, 868
Fnu4HI GCNGC 5 cut(s) 114, 301, 471, 984, 999
FokI GGATG 6 cut(s) 52, 89, 398, 578, 642, 905
FriOI GRGCYC 2 cut(s) 248, 1139
Fsp4HI GCNGC 5 cut(s) 114, 301, 471, 984, 999
FspBI CTAG 2 cut(s) 726, 1122
GlaI GCGC 2 cut(s) 547, 829
GluI GCNGC 5 cut(s) 114, 301, 471, 984, 999
GsaI CCCAGC 1 cut(s) 557
GsuI CTGGAG 1 cut(s) 597
HaeII RGCGCY 1 cut(s) 831
HaeIII GGCC 7 cut(s) 99, 197, 261, 303, 451, 817, 1137
HhaI GCGC 2 cut(s) 548, 830
Hin1II CATG 4 cut(s) 379, 854, 869, 1198
Hin6I GCGC 2 cut(s) 546, 828
HinP1I GCGC 2 cut(s) 546, 828
HinfI GANTC 8 cut(s) 230, 254, 423, 539, 626, 665, 897, 1100
HphI GGTGA 4 cut(s) 182, 323, 548, 938
Hpy166II GTNNAC 2 cut(s) 357, 803
Hpy188I TCNGA 7 cut(s) 109, 219, 364, 635, 892, 969, 1027
Hpy188III TCNNGA 5 cut(s) 340, 659, 726, 920, 1097
Hpy8I GTNNAC 2 cut(s) 357, 803
HpyAV CCTTC 2 cut(s) 122, 562
HpyCH4III ACNGT 4 cut(s) 372, 519, 1046, 1081
HpyCH4IV ACGT 2 cut(s) 359, 531
HpyCH4V TGCA 8 cut(s) 65, 113, 470, 646, 655, 839, 850, 986
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 267, 407, 457, 652, 836, 1122
HpyF3I CTNAG 2 cut(s) 306, 1002
HpySE526I ACGT 2 cut(s) 359, 531
Hsp92II CATG 4 cut(s) 379, 854, 869, 1198
HspAI GCGC 2 cut(s) 546, 828
Ksp22I TGATCA 2 cut(s) 328, 868
Kzo9I GATC 6 cut(s) 214, 328, 479, 501, 728, 868
LmnI GCTCC 2 cut(s) 772, 904
Lsp1109I GCAGC 3 cut(s) 125, 482, 970
LweI GCATC 5 cut(s) 74, 100, 633, 664, 916
MaeI CTAG 2 cut(s) 726, 1122
MaeII ACGT 2 cut(s) 359, 531
MaeIII GTNAC 4 cut(s) 170, 436, 536, 944
MalI GATC 6 cut(s) 216, 330, 481, 503, 730, 870
MboI GATC 6 cut(s) 214, 328, 479, 501, 728, 868
MboII GAAGA 3 cut(s) 428, 1018, 1045
MflI RGATCY 2 cut(s) 479, 728
MhlI GDGCHC 5 cut(s) 248, 297, 612, 967, 1139
MluCI AATT 7 cut(s) 7, 202, 343, 465, 819, 840, 872
MlyI GAGTC 7 cut(s) 239, 263, 533, 635, 659, 891, 1094
MmeI TCCRAC 2 cut(s) 31, 457
Mph1103I ATGCAT 1 cut(s) 648
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 3 cut(s) 765, 824, 1167
MslI CAYNNNNRTG 2 cut(s) 165, 380
MspA1I CMGCKG 4 cut(s) 17, 116, 598, 957
MspR9I CCNGG 3 cut(s) 24, 102, 1132
Mva1269I GAATGC 2 cut(s) 646, 1210
MvaI CCWGG 3 cut(s) 24, 102, 1132
MvnI CGCG 1 cut(s) 546
MwoI GCNNNNNNNGC 7 cut(s) 92, 267, 407, 457, 652, 836, 1122
NcoI CCATGG 1 cut(s) 1194
NdeII GATC 6 cut(s) 214, 328, 479, 501, 728, 868
NheI GCTAGC 1 cut(s) 1121
NlaIII CATG 4 cut(s) 379, 854, 869, 1198
NlaIV GGNNCC 6 cut(s) 106, 238, 262, 774, 1136, 1137
NmuCI GTSAC 3 cut(s) 170, 536, 944
NsiI ATGCAT 1 cut(s) 648
PasI CCCWGGG 1 cut(s) 23
PctI GAATGC 2 cut(s) 646, 1210
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 1 cut(s) 423
PflMI CCANNNNNTGG 1 cut(s) 447
PkrI GCNGC 5 cut(s) 115, 302, 472, 985, 1000
PleI GAGTC 7 cut(s) 238, 262, 533, 634, 659, 891, 1094
PpsI GAGTC 7 cut(s) 238, 262, 533, 634, 659, 891, 1094
PshBI ATTAAT 1 cut(s) 1167
Psp124BI GAGCTC 1 cut(s) 248
Psp1406I AACGTT 1 cut(s) 531
Psp6I CCWGG 3 cut(s) 22, 100, 1130
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 3 cut(s) 22, 100, 1130
PspN4I GGNNCC 6 cut(s) 106, 238, 262, 774, 1136, 1137
PspOMI GGGCCC 1 cut(s) 1135
PspPI GGNCC 6 cut(s) 98, 236, 260, 302, 1135, 1136
PsuI RGATCY 2 cut(s) 479, 728
PvuII CAGCTG 3 cut(s) 17, 116, 598
RsaI GTAC 5 cut(s) 13, 192, 521, 585, 1022
RsaNI GTAC 5 cut(s) 12, 191, 520, 584, 1021
RseI CAYNNNNRTG 2 cut(s) 165, 380
SacI GAGCTC 1 cut(s) 248
SaqAI TTAA 3 cut(s) 765, 824, 1167
SatI GCNGC 5 cut(s) 114, 301, 471, 984, 999
Sau3AI GATC 6 cut(s) 214, 328, 479, 501, 728, 868
Sau96I GGNCC 6 cut(s) 98, 236, 260, 302, 1135, 1136
SchI GAGTC 7 cut(s) 239, 263, 533, 635, 659, 891, 1094
ScrFI CCNGG 3 cut(s) 24, 102, 1132
SduI GDGCHC 5 cut(s) 248, 297, 612, 967, 1139
SfaNI GCATC 5 cut(s) 74, 100, 633, 664, 916
SinI GGWCC 1 cut(s) 236
SmiMI CAYNNNNRTG 2 cut(s) 165, 380
SmlI CTYRAG 4 cut(s) 209, 227, 241, 251
SmoI CTYRAG 4 cut(s) 209, 227, 241, 251
Sse9I AATT 7 cut(s) 7, 202, 343, 465, 819, 840, 872
SsiI CCGC 6 cut(s) 300, 544, 957, 998, 1054, 1176
SspMI CTAG 2 cut(s) 726, 1122
SstI GAGCTC 1 cut(s) 248
StyD4I CCNGG 3 cut(s) 22, 100, 1130
StyI CCWWGG 1 cut(s) 1194
TaaI ACNGT 4 cut(s) 372, 519, 1046, 1081
TaiI ACGT 2 cut(s) 362, 534
TaqI TCGA 1 cut(s) 1103
TasI AATT 7 cut(s) 7, 202, 343, 465, 819, 840, 872
TatI WGTACW 3 cut(s) 11, 190, 519
TauI GCSGC 2 cut(s) 303, 1001
TfiI GAWTC 1 cut(s) 423
Tru1I TTAA 3 cut(s) 765, 824, 1167
Tru9I TTAA 3 cut(s) 765, 824, 1167
TscAI CASTG 2 cut(s) 387, 522
TseFI GTSAC 3 cut(s) 170, 536, 944
TseI GCWGC 3 cut(s) 113, 470, 983
Tsp45I GTSAC 3 cut(s) 170, 536, 944
TspDTI ATGAA 1 cut(s) 867
TspGWI ACGGA 1 cut(s) 409
TspRI CASTG 2 cut(s) 387, 522
Van91I CCANNNNNTGG 1 cut(s) 447
VpaK11BI GGWCC 1 cut(s) 236
VspI ATTAAT 1 cut(s) 1167
XapI RAATTY 1 cut(s) 343
XbaI TCTAGA 1 cut(s) 725
XcmI CCANNNNNNNNNTGG 2 cut(s) 76, 230
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 2 cut(s) 726, 1122
Zsp2I ATGCAT 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.