Rmu_ssc0000226.1_g000032

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000226.1
OG0002246
Physical Location & Seq
Forward (+)
111099 .. 111752
654 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
atgcgtgttttcacatccatcatagcttcatttcttggtttccaagctaggtccttggttggccgtttcatcaccaccatacatgccacagttctggtcctgatattcagcatgattggcaagctcttgggtggtgccagcttcaatccctccaccactgtcagtttgtacgccgccggcctcaacccaggaacttccctgatctcaatggcagtccagttcccggctcaggcggccggcggagcaggtggcgccaaggccatcctgcaagtgatgcctaaaaagtatgaacattttgtcaaagggccttctctgaaagtggatgtgaaaagtggtgctattgctgaagggttgttcactttttctctttgcttttctctgctttgggttatgcttaggggtcctaagaatctttttctgcaaatatggttggttaaagtggccactgtgggattggctattgtgggaagtgggtacacagggccttctatgaaccctgctaatgcttttggctgggcttatgttgataactggcacaatacttgggagctgttctatgtttattgggttggtcctttaattggagcagctttggctgctagggtcttcaagattttgtttcctccaccagaaacaaaggagaagaaagcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

217

Amino Acids

23.32

Weight (kDa)

9.96

Isoelectric Point (pI)

21.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 25 - 202 7e-17 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
Acc36I ACCTGC 1 cut(s) 236
AccB1I GGYRCC 2 cut(s) 134, 251
AciI CCGC 3 cut(s) 174, 233, 240
AcoI YGGCCR 3 cut(s) 61, 234, 441
AcuI CTGAAG 1 cut(s) 366
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 2 cut(s) 170, 476
AfiI CCNNNNNNNGG 3 cut(s) 223, 229, 581
AgsI TTSAA 2 cut(s) 145, 610
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 6 cut(s) 26, 47, 124, 141, 550, 590
AluI AGCT 6 cut(s) 26, 47, 124, 141, 550, 590
AoxI GGCC 7 cut(s) 61, 178, 234, 258, 305, 441, 482
ApeKI GCWGC 2 cut(s) 587, 596
AspLEI GCGC 1 cut(s) 254
AspS9I GGNCC 6 cut(s) 51, 97, 305, 401, 482, 572
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 1 cut(s) 64
AvaII GGWCC 4 cut(s) 51, 97, 401, 572
BalI TGGCCA 1 cut(s) 443
BanI GGYRCC 2 cut(s) 134, 251
BbsI GAAGAC 1 cut(s) 598
BbvI GCAGC 2 cut(s) 583, 599
BccI CCATC 2 cut(s) 26, 269
BceAI ACGGC 1 cut(s) 48
BciT130I CCWGG 1 cut(s) 189
BcnI CCSGG 1 cut(s) 224
BfaI CTAG 2 cut(s) 48, 600
BfoI RGCGCY 1 cut(s) 255
BfuAI ACCTGC 1 cut(s) 236
BisI GCNGC 4 cut(s) 174, 234, 588, 597
BlsI GCNGC 4 cut(s) 175, 235, 589, 598
Bme1390I CCNGG 2 cut(s) 189, 224
Bme18I GGWCC 4 cut(s) 51, 97, 401, 572
BmgT120I GGNCC 6 cut(s) 51, 97, 305, 401, 482, 572
BmiI GGNNCC 3 cut(s) 136, 253, 402
BmrFI CCNGG 2 cut(s) 189, 224
BmsI GCATC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 598
Bpu10I CCTNAGC 2 cut(s) 228, 395
BpuMI CCSGG 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 3 cut(s) 54, 187, 255
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 229, 581
Bse118I RCCGGY 2 cut(s) 176, 236
Bse1I ACTGG 2 cut(s) 217, 536
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 3 cut(s) 54, 187, 255
BseGI GGATG 3 cut(s) 14, 261, 328
BseLI CCNNNNNNNGG 3 cut(s) 223, 229, 581
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 2 cut(s) 217, 536
BseX3I CGGCCG 1 cut(s) 234
BseXI GCAGC 2 cut(s) 583, 599
BseYI CCCAGC 1 cut(s) 513
Bsh1285I CGRYCG 1 cut(s) 237
BshFI GGCC 7 cut(s) 63, 180, 236, 260, 307, 443, 484
BshNI GGYRCC 2 cut(s) 134, 251
BsiEI CGRYCG 1 cut(s) 237
BsiSI CCGG 3 cut(s) 177, 224, 237
BslI CCNNNNNNNGG 3 cut(s) 223, 229, 581
BsnI GGCC 7 cut(s) 63, 180, 236, 260, 307, 443, 484
Bsp143I GATC 1 cut(s) 201
BspACI CCGC 3 cut(s) 174, 233, 240
BspANI GGCC 7 cut(s) 63, 180, 236, 260, 307, 443, 484
BspCNI CTCAG 1 cut(s) 241
BspLI GGNNCC 3 cut(s) 136, 253, 402
BspMI ACCTGC 1 cut(s) 236
BspT107I GGYRCC 2 cut(s) 134, 251
BsrFI RCCGGY 2 cut(s) 176, 236
BsrI ACTGG 2 cut(s) 217, 536
BssAI RCCGGY 2 cut(s) 176, 236
BssECI CCNNGG 3 cut(s) 54, 187, 255
BssMI GATC 1 cut(s) 201
BssNI GRCGYC 1 cut(s) 252
BssT1I CCWWGG 2 cut(s) 54, 255
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 3 cut(s) 91, 160, 448
BstACI GRCGYC 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 274
BstC8I GCNNGC 4 cut(s) 122, 139, 178, 238
BstDEI CTNAG 3 cut(s) 228, 395, 405
BstF5I GGATG 3 cut(s) 14, 261, 328
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 1 cut(s) 204
BstMBI GATC 1 cut(s) 201
BstMCI CGRYCG 1 cut(s) 237
BstMWI GCNNNNNNNGC 7 cut(s) 117, 233, 242, 251, 274, 593, 596
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 1 cut(s) 86
BstSCI CCNGG 2 cut(s) 187, 222
BstV1I GCAGC 2 cut(s) 583, 599
BstV2I GAAGAC 1 cut(s) 598
BstXI CCANNNNNNTGG 1 cut(s) 94
BstZI CGGCCG 1 cut(s) 234
BsuRI GGCC 7 cut(s) 63, 180, 236, 260, 307, 443, 484
BtsCI GGATG 3 cut(s) 14, 261, 328
BtsIMutI CAGTG 2 cut(s) 156, 444
BveI ACCTGC 1 cut(s) 236
Cac8I GCNNGC 4 cut(s) 122, 139, 178, 238
CfoI GCGC 1 cut(s) 254
Cfr10I RCCGGY 2 cut(s) 176, 236
Cfr13I GGNCC 6 cut(s) 51, 97, 305, 401, 482, 572
Csp6I GTAC 2 cut(s) 169, 475
CviAII CATG 2 cut(s) 83, 112
CviQI GTAC 2 cut(s) 169, 475
DdeI CTNAG 3 cut(s) 228, 395, 405
DinI GGCGCC 1 cut(s) 253
DpnI GATC 1 cut(s) 203
DpnII GATC 1 cut(s) 201
EaeI YGGCCR 3 cut(s) 61, 234, 441
EagI CGGCCG 1 cut(s) 234
EciI GGCGGA 1 cut(s) 255
EclXI CGGCCG 1 cut(s) 234
Eco130I CCWWGG 2 cut(s) 54, 255
Eco47I GGWCC 4 cut(s) 51, 97, 401, 572
Eco52I CGGCCG 1 cut(s) 234
Eco57I CTGAAG 1 cut(s) 366
EcoO109I RGGNCCY 4 cut(s) 51, 305, 401, 482
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 2 cut(s) 54, 255
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 2 cut(s) 54, 255
FaeI CATG 2 cut(s) 86, 115
FatI CATG 2 cut(s) 82, 111
Fnu4HI GCNGC 4 cut(s) 174, 234, 588, 597
FokI GGATG 2 cut(s) 248, 335
Fsp4HI GCNGC 4 cut(s) 174, 234, 588, 597
FspBI CTAG 2 cut(s) 48, 600
GlaI GCGC 1 cut(s) 253
GluI GCNGC 4 cut(s) 174, 234, 588, 597
GsaI CCCAGC 1 cut(s) 517
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 7 cut(s) 63, 180, 236, 260, 307, 443, 484
HapII CCGG 3 cut(s) 177, 224, 237
HhaI GCGC 1 cut(s) 254
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 2 cut(s) 86, 115
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HinfI GANTC 1 cut(s) 409
HpaII CCGG 3 cut(s) 177, 224, 237
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 2 cut(s) 357, 477
Hpy188I TCNGA 1 cut(s) 315
Hpy188III TCNNGA 2 cut(s) 100, 610
Hpy8I GTNNAC 2 cut(s) 357, 477
HpyAV CCTTC 3 cut(s) 318, 341, 495
HpyCH4III ACNGT 3 cut(s) 91, 160, 448
HpyCH4V TGCA 2 cut(s) 268, 421
HpyF10VI GCNNNNNNNGC 7 cut(s) 117, 233, 242, 251, 274, 593, 596
HpyF3I CTNAG 3 cut(s) 228, 395, 405
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 2 cut(s) 86, 115
HspAI GCGC 1 cut(s) 252
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 2 cut(s) 176, 236
KroNI GCCGGC 2 cut(s) 178, 238
Kzo9I GATC 1 cut(s) 201
LmnI GCTCC 3 cut(s) 242, 547, 584
Lsp1109I GCAGC 2 cut(s) 583, 599
LweI GCATC 1 cut(s) 264
MaeI CTAG 2 cut(s) 48, 600
MalI GATC 1 cut(s) 203
MboI GATC 1 cut(s) 201
MboII GAAGA 1 cut(s) 598
MlsI TGGCCA 1 cut(s) 443
MluCI AATT 1 cut(s) 579
MluNI TGGCCA 1 cut(s) 443
Mly113I GGCGCC 1 cut(s) 252
MnlI CCTC 3 cut(s) 160, 191, 633
Mox20I TGGCCA 1 cut(s) 443
MroNI GCCGGC 2 cut(s) 176, 236
MscI TGGCCA 1 cut(s) 443
MseI TTAA 2 cut(s) 435, 578
Msp20I TGGCCA 1 cut(s) 443
MspI CCGG 3 cut(s) 177, 224, 237
MspR9I CCNGG 2 cut(s) 189, 224
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 7 cut(s) 117, 233, 242, 251, 274, 593, 596
NaeI GCCGGC 2 cut(s) 178, 238
NarI GGCGCC 1 cut(s) 252
NciI CCSGG 1 cut(s) 224
NdeII GATC 1 cut(s) 201
NgoMIV GCCGGC 2 cut(s) 176, 236
NlaIII CATG 2 cut(s) 86, 115
NlaIV GGNNCC 3 cut(s) 136, 253, 402
NspI RCATGY 1 cut(s) 86
PaqCI CACCTGC 1 cut(s) 236
PdiI GCCGGC 2 cut(s) 178, 238
PfeI GAWTC 1 cut(s) 409
PkrI GCNGC 4 cut(s) 175, 235, 589, 598
PluTI GGCGCC 1 cut(s) 255
PpuMI RGGWCCY 2 cut(s) 51, 401
Psp5II RGGWCCY 2 cut(s) 51, 401
Psp6I CCWGG 1 cut(s) 187
PspFI CCCAGC 1 cut(s) 513
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 3 cut(s) 136, 253, 402
PspPI GGNCC 6 cut(s) 51, 97, 305, 401, 482, 572
PspPPI RGGWCCY 2 cut(s) 51, 401
RsaI GTAC 2 cut(s) 170, 476
RsaNI GTAC 2 cut(s) 169, 475
SaqAI TTAA 2 cut(s) 435, 578
SatI GCNGC 4 cut(s) 174, 234, 588, 597
Sau3AI GATC 1 cut(s) 201
Sau96I GGNCC 6 cut(s) 51, 97, 305, 401, 482, 572
ScrFI CCNGG 2 cut(s) 189, 224
SetI ASST 8 cut(s) 28, 49, 53, 126, 143, 250, 552, 592
SfaNI GCATC 1 cut(s) 264
SfoI GGCGCC 1 cut(s) 253
SinI GGWCC 4 cut(s) 51, 97, 401, 572
Sse9I AATT 1 cut(s) 579
SsiI CCGC 3 cut(s) 174, 233, 240
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 2 cut(s) 48, 600
StyD4I CCNGG 2 cut(s) 187, 222
StyI CCWWGG 2 cut(s) 54, 255
TaaI ACNGT 3 cut(s) 91, 160, 448
TasI AATT 1 cut(s) 579
TauI GCSGC 2 cut(s) 176, 236
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 2 cut(s) 435, 578
Tru9I TTAA 2 cut(s) 435, 578
TscAI CASTG 2 cut(s) 163, 451
TseI GCWGC 2 cut(s) 587, 596
TspDTI ATGAA 4 cut(s) 18, 58, 303, 506
TspRI CASTG 2 cut(s) 163, 451
VpaK11BI GGWCC 4 cut(s) 51, 97, 401, 572
XceI RCATGY 1 cut(s) 86
XcmI CCANNNNNNNNNTGG 1 cut(s) 451
XspI CTAG 2 cut(s) 48, 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.