Rmu_ssc0000286.1_g000021

Thymidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000286.1
OG0006534
Physical Location & Seq
Reverse (-)
143879 .. 144388
510 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
atgaaactcttcctcttcccatcttctctctccttctcttccctaattttcaatcccttttctcactgtaaccctaaatccatggcctccttcaaacccacaatccccctgaccagcgaatccgccccttcccgccgccccccctcgggcgaggtccacgtcatcatgggtcccatgttcgccggcaagaccaccgctctcctgcgccggatcaagtccgagggagccaacggcaggaatgtggcgatgataaaatcgagcaaggataacagatacgccgtcgattcggtggtgactcatgatggggcgaaatttccatgctgggctctgccggatctgtcctcctttaggcacaaatttggaggtgatgcttatgataaggtaattttggagctgggtaatatataa

Protein Analysis

135

Amino Acids

14.78

Weight (kDa)

9.74

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TK PF00265 50 - 106 1.6e-14 Thymidine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 197
AciI CCGC 4 cut(s) 123, 133, 136, 195
AclWI GGATC 2 cut(s) 218, 342
AcsI RAATTY 2 cut(s) 311, 356
AfiI CCNNNNNNNGG 4 cut(s) 145, 146, 234, 348
AgsI TTSAA 2 cut(s) 52, 94
AjiI CACGTC 1 cut(s) 160
AluBI AGCT 1 cut(s) 394
AluI AGCT 1 cut(s) 394
AlwI GGATC 2 cut(s) 218, 342
Ama87I CYCGRG 1 cut(s) 145
AoxI GGCC 1 cut(s) 84
ApoI RAATTY 2 cut(s) 311, 356
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 2 cut(s) 154, 170
AsuHPI GGTGA 2 cut(s) 304, 377
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 2 cut(s) 154, 170
BanII GRGCYC 1 cut(s) 328
BccI CCATC 2 cut(s) 28, 296
BceAI ACGGC 2 cut(s) 247, 263
BisI GCNGC 1 cut(s) 136
BlsI GCNGC 1 cut(s) 137
Bme18I GGWCC 2 cut(s) 154, 170
BmeT110I CYCGRG 1 cut(s) 145
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 2 cut(s) 154, 170
BmiI GGNNCC 3 cut(s) 171, 172, 226
BmsI GCATC 1 cut(s) 358
BsaJI CCNNGG 3 cut(s) 81, 144, 219
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 146, 234, 348
Bse118I RCCGGY 1 cut(s) 182
BseDI CCNNGG 3 cut(s) 81, 144, 219
BseLI CCNNNNNNNGG 4 cut(s) 145, 146, 234, 348
BseYI CCCAGC 2 cut(s) 321, 394
BshFI GGCC 1 cut(s) 86
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 3 cut(s) 183, 208, 332
BslFI GGGAC 1 cut(s) 156
BslI CCNNNNNNNGG 4 cut(s) 145, 146, 234, 348
BsmFI GGGAC 1 cut(s) 156
BsnI GGCC 1 cut(s) 86
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 1 cut(s) 328
Bsp143I GATC 2 cut(s) 210, 334
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 4 cut(s) 123, 133, 136, 195
BspANI GGCC 1 cut(s) 86
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 3 cut(s) 171, 172, 226
BspPI GGATC 2 cut(s) 218, 342
BsrBI CCGCTC 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 182
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 3 cut(s) 81, 144, 219
BssMI GATC 2 cut(s) 210, 334
BssT1I CCWWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 68
Bst6I CTCTTC 3 cut(s) 14, 20, 43
BstC8I GCNNGC 1 cut(s) 184
BstDSI CCRYGG 1 cut(s) 81
BstENI CCTNNNNNAGG 1 cut(s) 346
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 2 cut(s) 213, 337
BstMBI GATC 2 cut(s) 210, 334
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 1 cut(s) 86
BtgI CCRYGG 1 cut(s) 81
BtgZI GCGATG 1 cut(s) 260
BtrI CACGTC 1 cut(s) 160
BtsIMutI CAGTG 1 cut(s) 64
Cac8I GCNNGC 1 cut(s) 184
CciI TCATGA 1 cut(s) 298
CfoI GCGC 1 cut(s) 207
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 2 cut(s) 154, 170
CviAII CATG 5 cut(s) 82, 166, 175, 299, 318
CviJI RGCY 4 cut(s) 86, 227, 326, 394
CviKI_1 RGCY 4 cut(s) 86, 227, 326, 394
DpnI GATC 2 cut(s) 212, 336
DpnII GATC 2 cut(s) 210, 334
Eam1104I CTCTTC 3 cut(s) 14, 20, 43
EarI CTCTTC 3 cut(s) 14, 20, 43
EciI GGCGGA 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 328
Eco47I GGWCC 2 cut(s) 154, 170
Eco88I CYCGRG 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 346
EcoO109I RGGNCCY 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 1 cut(s) 328
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 5 cut(s) 85, 169, 178, 302, 321
FaiI YATR 8 cut(s) 83, 167, 176, 300, 319, 375, 404, 406
FaqI GGGAC 1 cut(s) 156
FatI CATG 5 cut(s) 81, 165, 174, 298, 317
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 1 cut(s) 136
FriOI GRGCYC 1 cut(s) 328
Fsp4HI GCNGC 1 cut(s) 136
GlaI GCGC 1 cut(s) 206
GluI GCNGC 1 cut(s) 136
GsaI CCCAGC 2 cut(s) 325, 398
HaeIII GGCC 1 cut(s) 86
HapII CCGG 3 cut(s) 183, 208, 332
HhaI GCGC 1 cut(s) 207
Hin1II CATG 5 cut(s) 85, 169, 178, 302, 321
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HinfI GANTC 3 cut(s) 119, 284, 295
HpaII CCGG 3 cut(s) 183, 208, 332
HphI GGTGA 2 cut(s) 304, 377
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 1 cut(s) 299
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 3 cut(s) 43, 100, 138
HpyCH4III ACNGT 1 cut(s) 68
HpyCH4IV ACGT 1 cut(s) 159
HpySE526I ACGT 1 cut(s) 159
Hsp92II CATG 5 cut(s) 85, 169, 178, 302, 321
HspAI GCGC 1 cut(s) 205
KflI GGGWCCC 1 cut(s) 170
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
Kzo9I GATC 2 cut(s) 210, 334
LmnI GCTCC 2 cut(s) 224, 391
LpnPI CCDG 9 cut(s) 122, 127, 196, 215, 220, 221, 307, 345, 380
LweI GCATC 1 cut(s) 358
MaeII ACGT 1 cut(s) 159
MaeIII GTNAC 2 cut(s) 68, 292
MalI GATC 2 cut(s) 212, 336
MbiI CCGCTC 1 cut(s) 197
MboI GATC 2 cut(s) 210, 334
MboII GAAGA 3 cut(s) 7, 15, 30
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 4 cut(s) 45, 311, 356, 384
MlyI GAGTC 1 cut(s) 289
MnlI CCTC 7 cut(s) 23, 97, 145, 154, 214, 352, 356
MroNI GCCGGC 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 8
MspI CCGG 3 cut(s) 183, 208, 332
NaeI GCCGGC 1 cut(s) 184
NcoI CCATGG 1 cut(s) 81
NdeII GATC 2 cut(s) 210, 334
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 5 cut(s) 85, 169, 178, 302, 321
NlaIV GGNNCC 3 cut(s) 171, 172, 226
NmuCI GTSAC 1 cut(s) 292
PagI TCATGA 1 cut(s) 298
PdiI GCCGGC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 2 cut(s) 119, 284
PkrI GCNGC 1 cut(s) 137
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
PpuMI RGGWCCY 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 170
PspFI CCCAGC 2 cut(s) 321, 394
PspN4I GGNNCC 3 cut(s) 171, 172, 226
PspPI GGNCC 2 cut(s) 154, 170
PspPPI RGGWCCY 1 cut(s) 170
PsuI RGATCY 1 cut(s) 334
SatI GCNGC 1 cut(s) 136
Sau3AI GATC 2 cut(s) 210, 334
Sau96I GGNCC 2 cut(s) 154, 170
SchI GAGTC 1 cut(s) 289
SduI GDGCHC 1 cut(s) 328
SetI ASST 5 cut(s) 156, 162, 367, 384, 396
SfaNI GCATC 1 cut(s) 358
SinI GGWCC 2 cut(s) 154, 170
Sse9I AATT 4 cut(s) 45, 311, 356, 384
SsiI CCGC 4 cut(s) 123, 133, 136, 195
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 68
TaiI ACGT 1 cut(s) 162
TaqI TCGA 2 cut(s) 257, 282
TasI AATT 4 cut(s) 45, 311, 356, 384
TauI GCSGC 1 cut(s) 138
TfiI GAWTC 2 cut(s) 119, 284
TscAI CASTG 1 cut(s) 71
TseFI GTSAC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 71
VpaK11BI GGWCC 2 cut(s) 154, 170
XagI CCTNNNNNAGG 1 cut(s) 346
XapI RAATTY 2 cut(s) 311, 356
XmnI GAANNNNTTC 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.