Rmu_ssc0000355.1_g000001

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000355.1
OG0002861
Physical Location & Seq
Forward (+)
1 .. 576
576 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
gcaggaattaaaatctccacaaaaaatgacttcgattcaccacgggactggtttggtcatggaagtcacacttcgtcaacagctgtaggtaaccatgtacccggcgcaagccatttcagatatgcaaaaggcacggcaagaggggtggcgccacgagcccatgttgccatatacaaggtcgcctgggcaacagactcggaaaagagtttcgctagtgatgtgcttgcaggcatggaccaagcaatttctgatggtgttgatataatgtcactgtctcttgcctttgacttccttccttatttcaacgatgtcattgccattgcttctctttctgtaatcgaacaaggaattgttgtggtctgtgctgctggaaatgacgggaaatacagtacaacatacaatggagcaccctggatcacaacagtaagagccggaacttttgatcgcaccttcactgcaacaatgactctagataatggtttgacggttgaagcaacatcgtacttccccgagagcgtttacattactgatgtgccattgtactacggcaaagacaatgcaacgaaagcaatatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

191

Amino Acids

20.41

Weight (kDa)

5.4

Isoelectric Point (pI)

18.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 7 - 149 2.6e-15 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 148
AclWI GGATC 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 4 cut(s) 99, 391, 503, 542
AgsI TTSAA 2 cut(s) 304, 491
AjnI CCWGG 2 cut(s) 182, 410
AluBI AGCT 1 cut(s) 83
AluI AGCT 1 cut(s) 83
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 1 cut(s) 279
AlwI GGATC 1 cut(s) 422
Ama87I CYCGRG 1 cut(s) 509
ApeKI GCWGC 1 cut(s) 365
AspLEI GCGC 2 cut(s) 107, 151
AspS9I GGNCC 1 cut(s) 235
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 30
AvaI CYCGRG 1 cut(s) 509
AvaII GGWCC 1 cut(s) 235
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 160
BauI CACGAG 1 cut(s) 153
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 1 cut(s) 352
BccI CCATC 1 cut(s) 245
BceAI ACGGC 2 cut(s) 150, 562
BcgI CGANNNNNNTGC 4 cut(s) 177, 211, 296, 330
BciT130I CCWGG 2 cut(s) 184, 412
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 279
BfaI CTAG 2 cut(s) 213, 470
BfmI CTRYAG 1 cut(s) 84
BfoI RGCGCY 1 cut(s) 152
BisI GCNGC 1 cut(s) 366
BlsI GCNGC 1 cut(s) 367
Bme1390I CCNGG 3 cut(s) 102, 184, 412
Bme18I GGWCC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 509
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 150
BmrFI CCNGG 3 cut(s) 102, 184, 412
BpuMI CCSGG 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 149
BsaJI CCNNGG 3 cut(s) 41, 183, 410
Bse1I ACTGG 1 cut(s) 53
Bse3DI GCAATG 2 cut(s) 312, 318
BseBI CCWGG 2 cut(s) 184, 412
BseDI CCNNGG 3 cut(s) 41, 183, 410
BseMI GCAATG 2 cut(s) 312, 318
BseNI ACTGG 1 cut(s) 53
BseXI GCAGC 1 cut(s) 352
BshNI GGYRCC 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 409
BsiHKCI CYCGRG 1 cut(s) 509
BsiSI CCGG 2 cut(s) 102, 432
BslFI GGGAC 1 cut(s) 59
BsmAI GTCTC 1 cut(s) 279
BsmFI GGGAC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 509
Bsp1286I GDGCHC 2 cut(s) 160, 409
Bsp143I GATC 2 cut(s) 414, 442
BspLI GGNNCC 1 cut(s) 150
BspPI GGATC 1 cut(s) 422
BspT107I GGYRCC 1 cut(s) 148
BsrDI GCAATG 2 cut(s) 312, 318
BsrI ACTGG 1 cut(s) 53
BssECI CCNNGG 3 cut(s) 41, 183, 410
BssMI GATC 2 cut(s) 414, 442
BssNI GRCGYC 1 cut(s) 149
BssSI CACGAG 1 cut(s) 153
Bst2BI CACGAG 1 cut(s) 153
Bst2UI CCWGG 2 cut(s) 184, 412
Bst4CI ACNGT 4 cut(s) 273, 389, 424, 487
BstACI GRCGYC 1 cut(s) 149
BstC8I GCNNGC 3 cut(s) 109, 225, 229
BstDSI CCRYGG 1 cut(s) 41
BstEII GGTNACC 1 cut(s) 89
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 2 cut(s) 107, 151
BstKTI GATC 2 cut(s) 417, 445
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 2 cut(s) 414, 442
BstMWI GCNNNNNNNGC 3 cut(s) 155, 164, 566
BstNI CCWGG 2 cut(s) 184, 412
BstPI GGTNACC 1 cut(s) 89
BstSCI CCNGG 3 cut(s) 100, 182, 410
BstSFI CTRYAG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 352
BstXI CCANNNNNNTGG 1 cut(s) 48
BtgI CCRYGG 1 cut(s) 41
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 269, 453
Cac8I GCNNGC 3 cut(s) 109, 225, 229
CfoI GCGC 2 cut(s) 107, 151
Cfr13I GGNCC 1 cut(s) 235
Csp6I GTAC 4 cut(s) 98, 390, 502, 541
CspCI CAANNNNNGTGG 2 cut(s) 126, 161
CviAII CATG 4 cut(s) 59, 95, 161, 232
CviJI RGCY 4 cut(s) 83, 111, 158, 431
CviKI_1 RGCY 4 cut(s) 83, 111, 158, 431
CviQI GTAC 4 cut(s) 98, 390, 502, 541
DinI GGCGCC 1 cut(s) 150
DpnI GATC 2 cut(s) 416, 444
DpnII GATC 2 cut(s) 414, 442
Eco24I GRGCYC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 235
Eco88I CYCGRG 1 cut(s) 509
Eco91I GGTNACC 1 cut(s) 89
EcoO65I GGTNACC 1 cut(s) 89
EcoRII CCWGG 2 cut(s) 182, 410
EcoT38I GRGCYC 1 cut(s) 160
EgeI GGCGCC 1 cut(s) 150
EheI GGCGCC 1 cut(s) 150
FaeI CATG 4 cut(s) 62, 98, 164, 235
FalI AAGNNNNNCTT 2 cut(s) 55, 87
FaqI GGGAC 1 cut(s) 59
FatI CATG 4 cut(s) 58, 94, 160, 231
Fnu4HI GCNGC 1 cut(s) 366
FriOI GRGCYC 1 cut(s) 160
Fsp4HI GCNGC 1 cut(s) 366
FspBI CTAG 2 cut(s) 213, 470
GlaI GCGC 2 cut(s) 106, 150
GluI GCNGC 1 cut(s) 366
HaeII RGCGCY 1 cut(s) 152
HapII CCGG 2 cut(s) 102, 432
HhaI GCGC 2 cut(s) 107, 151
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 4 cut(s) 62, 98, 164, 235
Hin6I GCGC 2 cut(s) 105, 149
HinP1I GCGC 2 cut(s) 105, 149
HincII GTYRAC 1 cut(s) 78
HindII GTYRAC 1 cut(s) 78
HinfI GANTC 3 cut(s) 35, 194, 466
HpaII CCGG 2 cut(s) 102, 432
HphI GGTGA 1 cut(s) 30
Hpy166II GTNNAC 2 cut(s) 78, 520
Hpy188I TCNGA 3 cut(s) 119, 199, 250
Hpy188III TCNNGA 1 cut(s) 470
Hpy8I GTNNAC 2 cut(s) 78, 520
HpyAV CCTTC 2 cut(s) 302, 460
HpyCH4III ACNGT 4 cut(s) 273, 389, 424, 487
HpyCH4V TGCA 4 cut(s) 125, 227, 458, 560
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 164, 566
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 4 cut(s) 62, 98, 164, 235
HspAI GCGC 2 cut(s) 105, 149
KasI GGCGCC 1 cut(s) 148
Kzo9I GATC 2 cut(s) 414, 442
LmnI GCTCC 1 cut(s) 404
LpnPI CCDG 9 cut(s) 34, 115, 169, 196, 213, 354, 397, 424, 445
Lsp1109I GCAGC 1 cut(s) 352
MaeI CTAG 2 cut(s) 213, 470
MaeIII GTNAC 3 cut(s) 65, 89, 267
MalI GATC 2 cut(s) 416, 444
MboI GATC 2 cut(s) 414, 442
MhlI GDGCHC 2 cut(s) 160, 409
MluCI AATT 3 cut(s) 6, 243, 348
Mly113I GGCGCC 1 cut(s) 149
MlyI GAGTC 2 cut(s) 188, 460
MnlI CCTC 1 cut(s) 134
MseI TTAA 1 cut(s) 9
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 2 cut(s) 102, 432
MspR9I CCNGG 3 cut(s) 102, 184, 412
MvaI CCWGG 2 cut(s) 184, 412
MwoI GCNNNNNNNGC 3 cut(s) 155, 164, 566
NarI GGCGCC 1 cut(s) 149
NciI CCSGG 1 cut(s) 102
NdeII GATC 2 cut(s) 414, 442
NlaIII CATG 4 cut(s) 62, 98, 164, 235
NlaIV GGNNCC 1 cut(s) 150
NmuCI GTSAC 2 cut(s) 65, 267
PfeI GAWTC 1 cut(s) 35
PkrI GCNGC 1 cut(s) 367
PleI GAGTC 2 cut(s) 188, 460
PluTI GGCGCC 1 cut(s) 152
PpsI GAGTC 2 cut(s) 188, 460
Psp6I CCWGG 2 cut(s) 182, 410
PspEI GGTNACC 1 cut(s) 89
PspGI CCWGG 2 cut(s) 182, 410
PspN4I GGNNCC 1 cut(s) 150
PspPI GGNCC 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 4 cut(s) 99, 391, 503, 542
RsaNI GTAC 4 cut(s) 98, 390, 502, 541
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 1 cut(s) 366
Sau3AI GATC 2 cut(s) 414, 442
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 2 cut(s) 188, 460
ScrFI CCNGG 3 cut(s) 102, 184, 412
SduI GDGCHC 2 cut(s) 160, 409
SetI ASST 4 cut(s) 85, 91, 180, 452
SfcI CTRYAG 1 cut(s) 84
SfoI GGCGCC 1 cut(s) 150
SinI GGWCC 1 cut(s) 235
Sse9I AATT 3 cut(s) 6, 243, 348
SspDI GGCGCC 1 cut(s) 148
SspMI CTAG 2 cut(s) 213, 470
StyD4I CCNGG 3 cut(s) 100, 182, 410
TaaI ACNGT 4 cut(s) 273, 389, 424, 487
TaqI TCGA 2 cut(s) 33, 339
TasI AATT 3 cut(s) 6, 243, 348
TatI WGTACW 2 cut(s) 389, 540
TfiI GAWTC 1 cut(s) 35
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 2 cut(s) 276, 460
TseFI GTSAC 2 cut(s) 65, 267
TseI GCWGC 1 cut(s) 365
Tsp45I GTSAC 2 cut(s) 65, 267
TspRI CASTG 2 cut(s) 276, 460
VpaK11BI GGWCC 1 cut(s) 235
XbaI TCTAGA 1 cut(s) 469
XspI CTAG 2 cut(s) 213, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.