Rroxscaffold_1G00058680

Haloacid dehalogenase-like hydrolase domain-containing protein At3g48420

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002631
Physical Location & Seq
Reverse (-)
80582934 .. 80676708
93775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058680.1

Sequence Viewer

Length: 342 bp
ATGAAAGCAGTTTCTGCTGAGAAACAAAGGATTGCAGAGGAGGTTGCGTCGATGCTAAAGTTGAATGTAGAGATTGATACTACCTCATCAGAAAGCTTACAGAAGATCATAGCTGCCTTGAGGGCAGGGGCAGAAAATGCTGGACTACCTGTCTCCAATTGTGTTCTAATTGCAGGAAGCCAGTCAGGAGTGGTAGGAGCTCAGCAAGTGGGCATGCCATGTGTTGTTTTAAGAAGCAGTTTGACGGCTAGAGCCGAGTTCCCTTCAGCAAATGCGATAATGGATGGATTTGGAGGAGCAGACCTAACTATTCCTAAACTTAAAAACAAGAAATGGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

113

Amino Acids

11.77

Weight (kDa)

8.68

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 249
AgsI TTSAA 1 cut(s) 64
AhdI GACNNNNNGTC 1 cut(s) 149
AluBI AGCT 3 cut(s) 96, 113, 200
AluI AGCT 3 cut(s) 96, 113, 200
Alw21I GWGCWC 1 cut(s) 202
Alw26I GTCTC 1 cut(s) 157
AlwNI CAGNNNCTG 1 cut(s) 14
ApeKI GCWGC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 202
BbvI GCAGC 1 cut(s) 100
BccI CCATC 1 cut(s) 278
BceAI ACGGC 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 157
BfaI CTAG 1 cut(s) 249
BglI GCCNNNNNGGC 1 cut(s) 122
BisI GCNGC 1 cut(s) 114
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 115
BmeRI GACNNNNNGTC 1 cut(s) 149
BmsI GCATC 1 cut(s) 42
Bpu1102I GCTNAGC 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 139
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bse1I ACTGG 1 cut(s) 181
BseGI GGATG 1 cut(s) 289
BseMII CTCAG 2 cut(s) 9, 215
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 2 cut(s) 53, 309
BseXI GCAGC 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 157
Bsp1286I GDGCHC 1 cut(s) 202
Bsp143I GATC 1 cut(s) 105
Bsp1720I GCTNAGC 1 cut(s) 201
BspCNI CTCAG 2 cut(s) 10, 214
BsrI ACTGG 1 cut(s) 181
BssMI GATC 1 cut(s) 105
BstAPI GCANNNNNTGC 2 cut(s) 14, 137
BstC8I GCNNGC 1 cut(s) 215
BstDEI CTNAG 2 cut(s) 18, 201
BstF5I GGATG 1 cut(s) 289
BstKTI GATC 1 cut(s) 108
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 1 cut(s) 105
BstMWI GCNNNNNNNGC 3 cut(s) 14, 122, 137
BstNSI RCATGY 1 cut(s) 217
BstV1I GCAGC 1 cut(s) 100
BtsCI GGATG 1 cut(s) 289
Cac8I GCNNGC 1 cut(s) 215
CaiI CAGNNNCTG 1 cut(s) 14
CseI GACGC 1 cut(s) 36
CviAII CATG 2 cut(s) 214, 219
CviJI RGCY 6 cut(s) 96, 113, 180, 200, 248, 254
CviKI_1 RGCY 6 cut(s) 96, 113, 180, 200, 248, 254
DdeI CTNAG 2 cut(s) 18, 201
DpnI GATC 1 cut(s) 107
DpnII GATC 1 cut(s) 105
DriI GACNNNNNGTC 1 cut(s) 149
Eam1105I GACNNNNNGTC 1 cut(s) 149
Ecl136II GAGCTC 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 200
EcoT38I GRGCYC 1 cut(s) 202
FaeI CATG 2 cut(s) 217, 222
FaiI YATR 4 cut(s) 110, 215, 220, 340
FatI CATG 2 cut(s) 213, 218
Fnu4HI GCNGC 1 cut(s) 114
FokI GGATG 1 cut(s) 296
FriOI GRGCYC 1 cut(s) 202
Fsp4HI GCNGC 1 cut(s) 114
FspBI CTAG 1 cut(s) 249
GluI GCNGC 1 cut(s) 114
HgaI GACGC 1 cut(s) 36
Hin1II CATG 2 cut(s) 217, 222
HindIII AAGCTT 1 cut(s) 94
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 1 cut(s) 186
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 1 cut(s) 273
HpyCH4V TGCA 2 cut(s) 35, 173
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 122, 137
HpyF3I CTNAG 2 cut(s) 18, 201
Hsp92II CATG 2 cut(s) 217, 222
Kzo9I GATC 1 cut(s) 105
LmnI GCTCC 2 cut(s) 197, 296
LpnPI CCDG 6 cut(s) 111, 126, 159, 162, 171, 194
Lsp1109I GCAGC 1 cut(s) 100
LweI GCATC 1 cut(s) 42
MaeI CTAG 1 cut(s) 249
MalI GATC 1 cut(s) 107
MboI GATC 1 cut(s) 105
MboII GAAGA 1 cut(s) 115
MfeI CAATTG 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 202
MluCI AATT 2 cut(s) 157, 168
MnlI CCTC 5 cut(s) 31, 34, 94, 114, 287
MseI TTAA 2 cut(s) 230, 321
MunI CAATTG 1 cut(s) 157
MwoI GCNNNNNNNGC 3 cut(s) 14, 122, 137
NdeII GATC 1 cut(s) 105
NlaIII CATG 2 cut(s) 217, 222
NmeAIII GCCGAG 1 cut(s) 280
NspI RCATGY 1 cut(s) 217
PaeI GCATGC 1 cut(s) 217
PkrI GCNGC 1 cut(s) 115
Psp124BI GAGCTC 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 14
SacI GAGCTC 1 cut(s) 202
SaqAI TTAA 2 cut(s) 230, 321
SatI GCNGC 1 cut(s) 114
Sau3AI GATC 1 cut(s) 105
SduI GDGCHC 1 cut(s) 202
SetI ASST 7 cut(s) 45, 86, 98, 115, 151, 202, 306
SfaNI GCATC 1 cut(s) 42
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
SphI GCATGC 1 cut(s) 217
Sse9I AATT 2 cut(s) 157, 168
SspMI CTAG 1 cut(s) 249
SstI GAGCTC 1 cut(s) 202
TaqI TCGA 1 cut(s) 50
TasI AATT 2 cut(s) 157, 168
Tru1I TTAA 2 cut(s) 230, 321
Tru9I TTAA 2 cut(s) 230, 321
TseI GCWGC 1 cut(s) 113
TspDTI ATGAA 1 cut(s) 17
XceI RCATGY 1 cut(s) 217
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.