Rroxscaffold_2G00121780

DNA excision repair protein ERCC-6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003840
Physical Location & Seq
Reverse (-)
54471461 .. 54481099
9639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00121780.1

Sequence Viewer

Length: 2997 bp
ATGGCGGAGAGGAAGAGCAGCAAGAAGCCCCAAAGCCTCAACGACAGTCACTATCGCCTCCTCCAAGACCTCAGTTCTGCTCCTCCCAAGCCCTCCGAGCAAGTAAAGGCGACTAAGGTGAGGATTGAAGGCGCGCGCCGTCTCTGTAAGATTCGAGTGGCTTCCGATGATGATGATGATCATGGTGAGGGCTTTAATGACGAGGACGCGCCCAGTTTTTCTGGAATTGCTGATTTTGAGTCGCCGCCGCAGCCGCAGAGGAGTGAGGTTGGAAATGGCGGTGGAAATGAGATTAGGGGAATTTTAGATGACTTGAGCTCGAGGCTTGAGTTTTTGTCAATCGAGAGGAAGGGAGCCAGAAAATCTAATAAGGTTGAGGATTCGGGGATTTCGAAGGAAGTTGATGGTGTAAAGAAAGAGAAAGTTGAGTATATGAATGCGGCGCCTTCGTTTTCGATTGCTTCTGACTTGTCTGATTCCTCTGCTGAGACCACTAAGACTGGTGGAAAGGGAGTTGAGAGTGTGGTGGATGAGTATGAAGAGAAAAGTCGTTTTCAATGCAAGTTTGAAGAGGCTGATGATGACAGAGATTGTGTGGTTGTGAGGGCTAAGAAGACGGATAAACAAGTGGGGAGGCGGGGTGGTGGTAGCTATAAGGAGTACTATGATTGTGATGAGGATAACGTGGTTGATGATTCTAGGGATGATTCTGTTCCGGAGGATGATGGTTCTATCACACTGAGTGGTCTCACATATACTTACAAATTACCTGGGAAGATTGCAAAGATGTTGTTTCCGCATCAGCGCGAAGGCTTGAAGTGGCTATGGGCTCTGCATTGCCAGGGTAAGGGAGGAATCTTAGGAGATGACATGGGTTTGGGTAAAACAATGCAGATTTGTGGCTATTTAGCTGGATTATTTCATTCACGCTTGATTAAAAGGGTAATGGTTGTGGCTCCTAAAACTCTACTTTCTCATTGGATAAAAGAATTATCAGCTGTGGGTCTCTCAGACAAGATAAGAGAATACTATGGGACATGTACAAAAGCTCGGAAGTATGAGCTTCAGTATGTACTTCAGGACAAAGGCATTCTTCTGACGACTTATGATATTGTGCGAGTCAACTCAAAATCTCTAAAAGGGAGTGACTATATTCTCGATGATGGAAGTGAGGATATGATATGGGATTATATGATACTTGATGAGGGTCATCTCATAAAGAATCCCAGTACACAAAGAGCCAAAAGTTTGCTAGACATACCCTGTTCACATCGCATTATAGTAAGTGGCACGCCATTGCAAAACAATCTGAAGGAGTTGTGGGCCTTATTTAACTTCTGTTGCCCTGAGCTACTGGGTGATAAGAACTGGTTCAAGGAAAGATTCGAGTCACGCATTTGTCGTGGAAATGAGAAAAATGCTTCAGATAGGGAGAAGCGTATTGGTTCCACGGTAGCACAGGAGCTTAGAGACTGTATTCAACCTTTTTTCCTGCGTCGCCTGAAGAATGAGGTGTTTAAGGAGGATAATGACCAAACAAATGCCACACTTTCTAAAAAGAATGAGATCATTGTTTGGCTAAGATTAACCAGCTGTCAGCGGAAACTTTATGAAGCATATTTAAAGAGTGAGCTGGTTCTTTCTGCTTTTGATGGGTCACCATTGGCTGCACTTACGATTCTGAAGAAAATATGTGATCATCCACTTCTATTGACAAAGCGAGCTGCTGAAGATGTACTAGAAGAGTTGGATTCATTGCTTAAGCCAGATGATGCTTGTATGGCAGAAAAACTAGCAATGCATATAGCAGATGTTGCTGAGAAAGAGGAGTTTGATGAGAACCATGCAAATCTCTCTTGCAAAATATCTTTTATAATGTCATTGTTGGATAACTTGATTCCTGAGGGGCATAATGTTCTCATCTTTTCCCAAACTAGAAAGATGCTTAATCTTATTCAGGAAACACTAATATCCAGCGGTTACAAGTTCCAACGCATTGATGGTACCACAAAAGCTACTGAAAGAATTAGGATTGTCAATGATTTCCAAGAAGGTATTGGGGCTCCCATATTTCTCCTGACTTCTCAAGTTGGTGGTTTGGGTCTTACACTTACAAGAGCTGATCGTGTGATAGTGGTTGATCCTGCTTGGAACCCGAGTACTGATAGCCAGAGTGTGGATCGTGCATATCGAATTGGGCAGAATAAGGATGTCATTGTATATAGGTTAATGACTTGTGCGACTGTTGAAGAAAAGATATACAGAAAACAGATTGCTGCAACTATTTACAAAGGAGGGTTGTTTAGAACTGCAACTGAGCAAAAAGAGCAGATTCGGTACTTTAGTCAACAGGATCTTCGTGAACTTTTCAGTCTCCCACAACAAGGTTTTGATGTTTCCCTTACTCAGAAGCAGCTAAATGAGGAGCATGATCAACAGCACACAATGGAAGTGTCTTTACAAGCCCATATAGAATTCTTGGAAACTCAAGGGATAGCTGGTGTTAGTAACCACAGTTTACTCTTCTCCAAGACAGCACCACCTCTGCCAGAGGTAGACGCGGAGCAGGAGGAAGTTGAAAGGGCTTTGTATTTTCAGTTGATAATATTGGGGCATTGTCCTTTATCTGGTTGCCTTTATTTTCTGGGTGCAGGATTAGGCGAACTTTACTTGCGTAGAAGCTCAACTTCAAGTTCGGCACCCGAATGTAATGTAAATGGGGCTGAATATGCTTTCAAGCCTAAGGATGTAATATTAAATAAGAAGATGCCTTCTTCACCAGCTGATGCGGGAAAAATGACAAAATCAGAGATTAAAGAGAACATCACTAGGCTGTCACAAACTCTTGCAAATAAGGCTTTAGTTTCAAGATTACCTGATAAGGGACAGAAAATACAGAGGCAAATTTCTGAATTGAATGCTGAGCTTCGTAGACTAGAGAAGGACGTAATTGACTTGGATGATGTGACTGAGGAGCTTAAAGTAGTGGTGATATAA

Protein Analysis

998

Amino Acids

112.15

Weight (kDa)

5.65

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 264 - 432 8.5e-07 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 267 - 568 2.6e-62 SNF2-related domain
AAA_34 PF13872 283 - 413 7.3e-07 P-loop containing NTP hydrolase pore-1
HDA2-3 PF11496 609 - 751 1.6e-06 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 624 - 733 5e-22 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1874
Acc65I GGTACC 1 cut(s) 2003
AccB1I GGYRCC 3 cut(s) 442, 2003, 2698
AccB7I CCANNNNNTGG 1 cut(s) 2176
AccI GTMKAC 2 cut(s) 2556, 2932
AccII CGCG 5 cut(s) 134, 136, 209, 807, 2561
AccIII TCCGGA 1 cut(s) 715
AclWI GGATC 3 cut(s) 2135, 2187, 2361
AcsI RAATTY 3 cut(s) 300, 2474, 2904
AcuI CTGAAG 7 cut(s) 1049, 1061, 1331, 1407, 1523, 1703, 1749
AcyI GRCGYC 1 cut(s) 443
AdeI CACNNNGTG 1 cut(s) 743
AfaI GTAC 8 cut(s) 662, 1042, 1074, 1231, 1737, 2005, 2161, 2339
AfiI CCNNNNNNNGG 5 cut(s) 847, 2176, 2384, 2627, 2882
AflII CTTAAG 1 cut(s) 1760
AflIII ACRYGT 1 cut(s) 1037
AjnI CCWGG 2 cut(s) 769, 840
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 6 cut(s) 146, 482, 752, 1010, 1464, 2378
AlwI GGATC 3 cut(s) 2135, 2187, 2361
Ama87I CYCGRG 2 cut(s) 319, 2155
Aor13HI TCCGGA 1 cut(s) 715
AoxI GGCC 1 cut(s) 1323
ApeKI GCWGC 6 cut(s) 18, 250, 1667, 1724, 2276, 2413
ApoI RAATTY 3 cut(s) 300, 2474, 2904
Asp700I GAANNNNTTC 1 cut(s) 1370
Asp718I GGTACC 1 cut(s) 2003
AspLEI GCGC 6 cut(s) 134, 136, 138, 211, 445, 807
AspS9I GGNCC 1 cut(s) 1323
AsuHPI GGTGA 5 cut(s) 130, 197, 1370, 1650, 2769
AsuII TTCGAA 1 cut(s) 392
AvaI CYCGRG 2 cut(s) 319, 2155
AxyI CCTNAGG 2 cut(s) 1902, 2742
BaeI ACNNNNGTAYC 2 cut(s) 2329, 2362
BanI GGYRCC 3 cut(s) 442, 2003, 2698
BanII GRGCYC 3 cut(s) 320, 832, 2065
BarI GAAGNNNNNNTAC 2 cut(s) 2442, 2474
BbsI GAAGAC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 6 cut(s) 30, 262, 1654, 1711, 2263, 2425
BccI CCATC 5 cut(s) 398, 719, 1157, 1646, 1994
BceAI ACGGC 1 cut(s) 123
BciT130I CCWGG 2 cut(s) 771, 842
BclI TGATCA 3 cut(s) 178, 1696, 2431
BcoDI GTCTC 6 cut(s) 146, 482, 752, 1010, 1464, 2378
BfaI CTAG 7 cut(s) 699, 1253, 1739, 1793, 1935, 2829, 2936
BfoI RGCGCY 1 cut(s) 446
BfrI CTTAAG 1 cut(s) 1760
BlpI GCTNAGC 1 cut(s) 2922
BmcAI AGTACT 2 cut(s) 662, 2161
Bme1390I CCNGG 2 cut(s) 771, 842
BmeT110I CYCGRG 2 cut(s) 319, 2155
BmgT120I GGNCC 1 cut(s) 1323
BmiI GGNNCC 8 cut(s) 355, 444, 957, 1447, 2005, 2064, 2153, 2700
BmrFI CCNGG 2 cut(s) 771, 842
BmrI ACTGGG 3 cut(s) 207, 1221, 1364
BmsI GCATC 5 cut(s) 808, 1762, 1932, 2757, 2776
BmuI ACTGGG 3 cut(s) 207, 1221, 1364
BpiI GAAGAC 1 cut(s) 620
Bpu10I CCTNAGC 1 cut(s) 1347
Bpu1102I GCTNAGC 1 cut(s) 2922
Bpu14I TTCGAA 1 cut(s) 392
BpuEI CTTGAG 4 cut(s) 334, 347, 2070, 2472
BsaBI GATNNNNATC 1 cut(s) 177
BsaHI GRCGYC 1 cut(s) 443
BsaI GGTCTC 3 cut(s) 482, 752, 1010
BsaJI CCNNGG 3 cut(s) 770, 841, 1449
BsaWI WCCGGW 1 cut(s) 715
BsaXI ACNNNNNCTCC 2 cut(s) 625, 655
Bsc4I CCNNNNNNNGG 5 cut(s) 847, 2176, 2384, 2627, 2882
Bse1I ACTGG 5 cut(s) 213, 505, 1227, 1359, 1373
Bse21I CCTNAGG 2 cut(s) 1902, 2742
Bse3DI GCAATG 4 cut(s) 835, 1295, 1754, 1803
Bse8I GATNNNNATC 1 cut(s) 177
BseAI TCCGGA 1 cut(s) 715
BseBI CCWGG 2 cut(s) 771, 842
BseDI CCNNGG 3 cut(s) 770, 841, 1449
BseGI GGATG 7 cut(s) 535, 709, 727, 1699, 2215, 2752, 2965
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 5 cut(s) 847, 2176, 2384, 2627, 2882
BseMI GCAATG 4 cut(s) 835, 1295, 1754, 1803
BseNI ACTGG 5 cut(s) 213, 505, 1227, 1359, 1373
BsePI GCGCGC 2 cut(s) 132, 134
BseRI GAGGAG 6 cut(s) 50, 72, 274, 1841, 2438, 2987
BseXI GCAGC 6 cut(s) 30, 262, 1654, 1711, 2263, 2425
BsgI GTGCAG 2 cut(s) 1653, 2670
Bsh1236I CGCG 5 cut(s) 134, 136, 209, 807, 2561
BshFI GGCC 1 cut(s) 1325
BshNI GGYRCC 3 cut(s) 442, 2003, 2698
BsiHKAI GWGCWC 1 cut(s) 320
BsiHKCI CYCGRG 2 cut(s) 319, 2155
BsiSI CCGG 1 cut(s) 716
BslFI GGGAC 2 cut(s) 1048, 2898
BslI CCNNNNNNNGG 5 cut(s) 847, 2176, 2384, 2627, 2882
BsmAI GTCTC 6 cut(s) 146, 482, 752, 1010, 1464, 2378
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 2 cut(s) 1048, 2898
BsmI GAATGC 3 cut(s) 442, 1089, 2923
BsnI GGCC 1 cut(s) 1325
Bso31I GGTCTC 3 cut(s) 482, 752, 1010
BsoBI CYCGRG 2 cut(s) 319, 2155
Bsp119I TTCGAA 1 cut(s) 392
Bsp1286I GDGCHC 3 cut(s) 320, 832, 2065
Bsp13I TCCGGA 1 cut(s) 715
Bsp1407I TGTACA 1 cut(s) 1040
Bsp143I GATC 8 cut(s) 178, 1566, 1696, 2122, 2140, 2179, 2353, 2431
Bsp1720I GCTNAGC 1 cut(s) 2922
BspANI GGCC 1 cut(s) 1325
BspEI TCCGGA 1 cut(s) 715
BspFNI CGCG 5 cut(s) 134, 136, 209, 807, 2561
BspLI GGNNCC 8 cut(s) 355, 444, 957, 1447, 2005, 2064, 2153, 2700
BspPI GGATC 3 cut(s) 2135, 2187, 2361
BspQI GCTCTTC 1 cut(s) 8
BspT104I TTCGAA 1 cut(s) 392
BspT107I GGYRCC 3 cut(s) 442, 2003, 2698
BspTI CTTAAG 1 cut(s) 1760
BspTNI GGTCTC 3 cut(s) 482, 752, 1010
BsrDI GCAATG 4 cut(s) 835, 1295, 1754, 1803
BsrGI TGTACA 1 cut(s) 1040
BsrI ACTGG 5 cut(s) 213, 505, 1227, 1359, 1373
BssECI CCNNGG 3 cut(s) 770, 841, 1449
BssHII GCGCGC 2 cut(s) 132, 134
BssMI GATC 8 cut(s) 178, 1566, 1696, 2122, 2140, 2179, 2353, 2431
BssNI GRCGYC 1 cut(s) 443
Bst2UI CCWGG 2 cut(s) 771, 842
Bst4CI ACNGT 5 cut(s) 47, 1453, 1475, 2245, 2516
Bst6I CTCTTC 5 cut(s) 8, 534, 564, 1737, 2528
BstACI GRCGYC 1 cut(s) 443
BstAFI CTTAAG 1 cut(s) 1760
BstAPI GCANNNNNTGC 1 cut(s) 1814
BstAUI TGTACA 1 cut(s) 1040
BstBI TTCGAA 1 cut(s) 392
BstC8I GCNNGC 4 cut(s) 134, 136, 1292, 1722
BstDSI CCRYGG 1 cut(s) 1449
BstEII GGTNACC 1 cut(s) 1656
BstF5I GGATG 7 cut(s) 535, 709, 727, 1699, 2215, 2752, 2965
BstFNI CGCG 5 cut(s) 134, 136, 209, 807, 2561
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 6 cut(s) 134, 136, 138, 211, 445, 807
BstKTI GATC 8 cut(s) 181, 1569, 1699, 2125, 2143, 2182, 2356, 2434
BstMAI GTCTC 6 cut(s) 146, 482, 752, 1010, 1464, 2378
BstMBI GATC 8 cut(s) 178, 1566, 1696, 2122, 2140, 2179, 2353, 2431
BstMWI GCNNNNNNNGC 8 cut(s) 97, 250, 253, 1781, 1814, 2326, 2729, 2855
BstNI CCWGG 2 cut(s) 771, 842
BstNSI RCATGY 1 cut(s) 1041
BstPI GGTNACC 1 cut(s) 1656
BstSCI CCNGG 2 cut(s) 769, 840
BstUI CGCG 5 cut(s) 134, 136, 209, 807, 2561
BstV1I GCAGC 6 cut(s) 30, 262, 1654, 1711, 2263, 2425
BstV2I GAAGAC 1 cut(s) 620
BstX2I RGATCY 1 cut(s) 2353
BstYI RGATCY 1 cut(s) 2353
Bsu36I CCTNAGG 2 cut(s) 1902, 2742
BsuRI GGCC 1 cut(s) 1325
BtgI CCRYGG 1 cut(s) 1449
BtgZI GCGATG 1 cut(s) 1256
BtsCI GGATG 7 cut(s) 535, 709, 727, 1699, 2215, 2752, 2965
BtsIMutI CAGTG 1 cut(s) 737
Cac8I GCNNGC 4 cut(s) 134, 136, 1292, 1722
CfoI GCGC 6 cut(s) 134, 136, 138, 211, 445, 807
Cfr13I GGNCC 1 cut(s) 1323
CseI GACGC 3 cut(s) 215, 1484, 2567
Csp6I GTAC 8 cut(s) 661, 1041, 1073, 1230, 1736, 2004, 2160, 2338
CspCI CAANNNNNGTGG 2 cut(s) 1692, 1727
CviAII CATG 5 cut(s) 182, 871, 1038, 1844, 2429
CviQI GTAC 8 cut(s) 661, 1041, 1073, 1230, 1736, 2004, 2160, 2338
DinI GGCGCC 1 cut(s) 444
DpnI GATC 8 cut(s) 180, 1568, 1698, 2124, 2142, 2181, 2355, 2433
DpnII GATC 8 cut(s) 178, 1566, 1696, 2122, 2140, 2179, 2353, 2431
DraI TTTAAA 1 cut(s) 1623
DraIII CACNNNGTG 1 cut(s) 743
Eam1104I CTCTTC 5 cut(s) 8, 534, 564, 1737, 2528
EarI CTCTTC 5 cut(s) 8, 534, 564, 1737, 2528
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 318
Eco24I GRGCYC 3 cut(s) 320, 832, 2065
Eco31I GGTCTC 3 cut(s) 482, 752, 1010
Eco53kI GAGCTC 1 cut(s) 318
Eco57I CTGAAG 7 cut(s) 1049, 1061, 1331, 1407, 1523, 1703, 1749
Eco81I CCTNAGG 2 cut(s) 1902, 2742
Eco88I CYCGRG 2 cut(s) 319, 2155
Eco91I GGTNACC 1 cut(s) 1656
EcoICRI GAGCTC 1 cut(s) 318
EcoO65I GGTNACC 1 cut(s) 1656
EcoRI GAATTC 1 cut(s) 2474
EcoRII CCWGG 2 cut(s) 769, 840
EcoT22I ATGCAT 1 cut(s) 1803
EcoT38I GRGCYC 3 cut(s) 320, 832, 2065
EgeI GGCGCC 1 cut(s) 444
EheI GGCGCC 1 cut(s) 444
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 5 cut(s) 185, 874, 1041, 1847, 2432
FalI AAGNNNNNCTT 4 cut(s) 1077, 1109, 2671, 2703
FaqI GGGAC 2 cut(s) 1048, 2898
FatI CATG 5 cut(s) 181, 870, 1037, 1843, 2428
FauI CCCGC 2 cut(s) 630, 2782
FbaI TGATCA 3 cut(s) 178, 1696, 2431
FblI GTMKAC 2 cut(s) 2556, 2932
FokI GGATG 7 cut(s) 542, 716, 734, 1686, 2222, 2759, 2972
FriOI GRGCYC 3 cut(s) 320, 832, 2065
FspBI CTAG 7 cut(s) 699, 1253, 1739, 1793, 1935, 2829, 2936
GlaI GCGC 6 cut(s) 133, 135, 137, 210, 444, 806
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 1 cut(s) 1325
HapII CCGG 1 cut(s) 716
HgaI GACGC 3 cut(s) 215, 1484, 2567
HhaI GCGC 6 cut(s) 134, 136, 138, 211, 445, 807
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 5 cut(s) 185, 874, 1041, 1847, 2432
Hin6I GCGC 6 cut(s) 132, 134, 136, 209, 443, 805
HinP1I GCGC 6 cut(s) 132, 134, 136, 209, 443, 805
HincII GTYRAC 2 cut(s) 1123, 2348
HindII GTYRAC 2 cut(s) 1123, 2348
HpaII CCGG 1 cut(s) 716
HphI GGTGA 5 cut(s) 130, 197, 1370, 1650, 2769
Hpy166II GTNNAC 8 cut(s) 1123, 1232, 1268, 2348, 2363, 2519, 2557, 2933
Hpy8I GTNNAC 8 cut(s) 1123, 1232, 1268, 2348, 2363, 2519, 2557, 2933
Hpy99I CGWCG 1 cut(s) 1500
HpyAV CCTTC 9 cut(s) 122, 343, 388, 456, 803, 1306, 2045, 2781, 2935
HpyCH4III ACNGT 5 cut(s) 47, 1453, 1475, 2245, 2516
HpyCH4IV ACGT 2 cut(s) 684, 2946
HpyF10VI GCNNNNNNNGC 8 cut(s) 97, 250, 253, 1781, 1814, 2326, 2729, 2855
HpySE526I ACGT 2 cut(s) 684, 2946
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 5 cut(s) 185, 874, 1041, 1847, 2432
HspAI GCGC 6 cut(s) 132, 134, 136, 209, 443, 805
KasI GGCGCC 1 cut(s) 442
Kpn2I TCCGGA 1 cut(s) 715
KpnI GGTACC 1 cut(s) 2007
Ksp22I TGATCA 3 cut(s) 178, 1696, 2431
Kzo9I GATC 8 cut(s) 178, 1566, 1696, 2122, 2140, 2179, 2353, 2431
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 8 cut(s) 85, 353, 961, 1462, 2068, 2425, 2563, 2974
Lsp1109I GCAGC 6 cut(s) 30, 262, 1654, 1711, 2263, 2425
LweI GCATC 5 cut(s) 808, 1762, 1932, 2757, 2776
MaeI CTAG 7 cut(s) 699, 1253, 1739, 1793, 1935, 2829, 2936
MaeII ACGT 2 cut(s) 684, 2946
MaeIII GTNAC 8 cut(s) 47, 1145, 1389, 1656, 1979, 2507, 2835, 2965
MalI GATC 8 cut(s) 180, 1568, 1698, 2124, 2142, 2181, 2355, 2433
MboI GATC 8 cut(s) 178, 1566, 1696, 2122, 2140, 2179, 2353, 2431
MflI RGATCY 1 cut(s) 2353
MhlI GDGCHC 3 cut(s) 320, 832, 2065
Mly113I GGCGCC 1 cut(s) 443
MlyI GAGTC 3 cut(s) 248, 1128, 1397
MmeI TCCRAC 4 cut(s) 250, 1728, 1866, 2014
Mph1103I ATGCAT 1 cut(s) 1803
MroI TCCGGA 1 cut(s) 715
MroXI GAANNNNTTC 1 cut(s) 1370
MslI CAYNNNNRTG 1 cut(s) 2704
MspA1I CMGCKG 5 cut(s) 998, 1593, 1600, 1977, 2783
MspCI CTTAAG 1 cut(s) 1760
MspI CCGG 1 cut(s) 716
MspR9I CCNGG 2 cut(s) 771, 842
Mva1269I GAATGC 3 cut(s) 442, 1089, 2923
MvaI CCWGG 2 cut(s) 771, 842
MvnI CGCG 5 cut(s) 134, 136, 209, 807, 2561
MwoI GCNNNNNNNGC 8 cut(s) 97, 250, 253, 1781, 1814, 2326, 2729, 2855
NarI GGCGCC 1 cut(s) 443
NdeII GATC 8 cut(s) 178, 1566, 1696, 2122, 2140, 2179, 2353, 2431
NlaIII CATG 5 cut(s) 185, 874, 1041, 1847, 2432
NlaIV GGNNCC 8 cut(s) 355, 444, 957, 1447, 2005, 2064, 2153, 2700
NmuCI GTSAC 6 cut(s) 47, 1145, 1389, 1656, 2835, 2965
NsiI ATGCAT 1 cut(s) 1803
NspI RCATGY 1 cut(s) 1041
NspV TTCGAA 1 cut(s) 392
PaeR7I CTCGAG 1 cut(s) 319
PauI GCGCGC 2 cut(s) 132, 134
PciI ACATGT 1 cut(s) 1037
PciSI GCTCTTC 1 cut(s) 8
PcsI WCGNNNNNNNCGW 3 cut(s) 389, 1399, 2188
PctI GAATGC 3 cut(s) 442, 1089, 2923
PdmI GAANNNNTTC 1 cut(s) 1370
PflMI CCANNNNNTGG 1 cut(s) 2176
PleI GAGTC 3 cut(s) 247, 1127, 1396
PluTI GGCGCC 1 cut(s) 446
PpsI GAGTC 3 cut(s) 247, 1127, 1396
PscI ACATGT 1 cut(s) 1037
PsiI TTATAA 1 cut(s) 1874
Psp124BI GAGCTC 1 cut(s) 320
Psp6I CCWGG 2 cut(s) 769, 840
PspEI GGTNACC 1 cut(s) 1656
PspGI CCWGG 2 cut(s) 769, 840
PspN4I GGNNCC 8 cut(s) 355, 444, 957, 1447, 2005, 2064, 2153, 2700
PspPI GGNCC 1 cut(s) 1323
PspXI VCTCGAGB 1 cut(s) 319
PsuI RGATCY 1 cut(s) 2353
PteI GCGCGC 2 cut(s) 132, 134
PvuII CAGCTG 3 cut(s) 998, 1593, 2783
RsaI GTAC 8 cut(s) 662, 1042, 1074, 1231, 1737, 2005, 2161, 2339
RsaNI GTAC 8 cut(s) 661, 1041, 1073, 1230, 1736, 2004, 2160, 2338
RseI CAYNNNNRTG 1 cut(s) 2704
SacI GAGCTC 1 cut(s) 320
SapI GCTCTTC 1 cut(s) 8
Sau3AI GATC 8 cut(s) 178, 1566, 1696, 2122, 2140, 2179, 2353, 2431
Sau96I GGNCC 1 cut(s) 1323
ScaI AGTACT 2 cut(s) 662, 2161
SchI GAGTC 3 cut(s) 248, 1128, 1397
ScrFI CCNGG 2 cut(s) 771, 842
SduI GDGCHC 3 cut(s) 320, 832, 2065
SfaNI GCATC 5 cut(s) 808, 1762, 1932, 2757, 2776
SfoI GGCGCC 1 cut(s) 444
Sfr274I CTCGAG 1 cut(s) 319
SfuI TTCGAA 1 cut(s) 392
SlaI CTCGAG 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 2704
SmlI CTYRAG 6 cut(s) 313, 319, 326, 1760, 2085, 2487
SmoI CTYRAG 6 cut(s) 313, 319, 326, 1760, 2085, 2487
SspDI GGCGCC 1 cut(s) 442
SspI AATATT 2 cut(s) 2607, 2754
SspMI CTAG 7 cut(s) 699, 1253, 1739, 1793, 1935, 2829, 2936
SstI GAGCTC 1 cut(s) 320
StyD4I CCNGG 2 cut(s) 769, 840
TaaI ACNGT 5 cut(s) 47, 1453, 1475, 2245, 2516
TaiI ACGT 2 cut(s) 687, 2949
TaqI TCGA 8 cut(s) 154, 320, 342, 392, 455, 1158, 1386, 2191
TatI WGTACW 6 cut(s) 660, 1040, 1072, 1229, 1735, 2159
TauI GCSGC 4 cut(s) 247, 250, 256, 443
TscAI CASTG 1 cut(s) 744
TseFI GTSAC 6 cut(s) 47, 1145, 1389, 1656, 2835, 2965
TseI GCWGC 6 cut(s) 18, 250, 1667, 1724, 2276, 2413
Tsp45I GTSAC 6 cut(s) 47, 1145, 1389, 1656, 2835, 2965
TspDTI ATGAA 5 cut(s) 449, 552, 911, 1626, 1743
TspGWI ACGGA 1 cut(s) 632
TspRI CASTG 1 cut(s) 744
Van91I CCANNNNNTGG 1 cut(s) 2176
Vha464I CTTAAG 1 cut(s) 1760
XapI RAATTY 3 cut(s) 300, 2474, 2904
XceI RCATGY 1 cut(s) 1041
XcmI CCANNNNNNNNNTGG 2 cut(s) 1997, 2054
XhoI CTCGAG 1 cut(s) 319
XmiI GTMKAC 2 cut(s) 2556, 2932
XmnI GAANNNNTTC 1 cut(s) 1370
XspI CTAG 7 cut(s) 699, 1253, 1739, 1793, 1935, 2829, 2936
ZrmI AGTACT 2 cut(s) 662, 2161
Zsp2I ATGCAT 1 cut(s) 1803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.