Rw2G023540

DNA excision repair protein ERCC-6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0003840
Physical Location & Seq
Forward (+)
34415126 .. 34424268
9143 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G023540.1

Sequence Viewer

Length: 2880 bp
ATGGCGGAGAGGAAGAGCAGCAAGAAGCCTCAAAGCCTCAACGACAGTCACTATCGCCTCCTCCAAGACCTCAGTTCTGCTCCTCCCAAGCCCTCCGGTACTCAAGGAAAGGCGACTAAGGTGAGGATTGAAGGCGCGCGCCGTCTCTGTAAGATTCGAGTGGCTTCCGATGATGACGATGATCATGGTGAGGGCTTTAATGATGACGACGCGCCCAGTTTCTCCGGGATTACTGATTTTGAGTCGCCGCCGCAGCCGCAGAGGAGTGAAGTTGGAAATGGCGGTGGAAATGAGATTAGGGGGATTTTAGATGACTTGAGCTCCAGGCTTGAGTTTTTGTCAATCGAGAGGAAGGGAGCCAGAAAATCTAATAAGGTTGAAGATTCGGGGATTTCGAAGGAAGTTGATCGGGTAAAGAAAGAGAAAGTTGAGTACAAGAATGCGGCGCCTTCGTTTTCGATTGCTTCTGACTTGTCTGATTCCTCTGCTGAGACCAGTAAAACTGGTGGAAAGGGAGTTGAGAGTGTGGTGGATGAATATGAAGAGAAAAGTCATTTTCAATGCAAGTTCGAAGAGGCTGATGATGACAGAGATTGTGTGGTTGTGAGGGCTAAGAAGACGGATAAACAAGTGGGGAGGCGGGGTGGTGGTAGCTATAAAGAGTACTATGATTGTAATGAGGATAACGTGGTTGATGATTCTGTTCCGGAGGATGATGGTTCTATCACTCTGAGTGGTCTCACATATACTTACAAATTACCTGGGAAGATTGCAAAGATGTTGTTTCCGCATCAGCGCGAAGGCTTGAAGTGGCTATGGGCTCTGCATTGCCAGGGTAAGGGAGGAATCTTAGGAGATGACATGGGTTTGGGTAAAACAATGCAGATTTGTGGCTATTTAGCTGGATTATTTCATTCACGCTTGGTTAAAAGGGTAATGGTTGTGGCTCCTAAAACACTACTTTCTCATTGGATAAAAGAATTATCAGCTGTGGGTCTCTCAGACAAGATAAGAGAATACTATGGGACATGTACAAAAGCTCGGAAGTATGAGCTTCAGTATGTACTTCAGGACAAAGGCATTCTTCTGACGACTTATGATATTGTGCGAGTCAACTCAAAATCTCTAAAAGGGAGTGACTATATTCTCGATGATGGAAGTGAGGATATGATATGGGATTATATGATACTTGATGAGGGTCATCTCATAAAGAATCCCAGTACACAAAGAGCCAAAAGTTTGCTAGATATACCCTGTTCACATCGCATTATAGTAAGTGGCACGCCATTGCAAAACAATCTGAAGGAGTTGTGGGCCTTATTTAACTTCTGTTGCCCTGAGCTACTGGGTGATAAGAACTGGTTCAAGGAAAGATTCGAGTCACGCATTTGTCGTGGAAATGAGAAAAATGCTTCAGATAGGGAGAAGCGTATTGGTTCCACGGTAGCACAGGAGCTCAGAGACCGTATTCAACCTTTTTTCCTGCGTCGCCTGAAGAATGAGGTGTTTAAGGAGGATAATGACCAAACAAATGCCACACTTTCTAAAAAGAATGAGATCATTGTTTGGCTAAGATTAACCAGCTGTCAGCGGAAACTTTATGAAGCATATTTAAAGAGTGAGCTGGTTCTTTCTGCTTTTGATGGGTCACCATTGGCTGCACTTACGATTCTGAAGAAAATATGTGATCATCCACTTCTATTGACAAAGCGAGCTGCTGAAGATGTACTAGAAGAGTTGGATTCATTGCTTAAGCCAGATGATGCTTGTATGGCAGAAAAACTAGCAATGCATATATTTGATGAGAACCATGCAAATCTCTCTTGCAAAATATCTTTTATAATGTCATTGTTGGATAACTTGATTCCTGAGGGGCATAATGTTCTCATCTTTTCCCAAACTAGAAAGATGCTTAATCTTATTCAGGAAACACTGATATCCAGCGGTTACAAGTTCCAACGCATTGATGGTACCACAAAAGCTACTGAAAGAATTAGGATTGTTAATGATTTCCAAGAAGGTATTGGGGCTCCCATATTTCTCCTGACTTCTCAAGTTGGTGGTTTGGGTCTTACACTTACAAGAGCTGATCGTGTGATAGTGGTTGATCCTGCTTGGAACCCGAGTACTGATAGCCAGAGTGTGGATCGTGCATATCGAATTGGGCAGAATAAGGATGTCATTGTATATAGATTAATGACTTGTGCGACTGTTGAAGAAAAGATATATAGAAAACAGATTTACAAAGGGGGGTTGTTTAGAACTGCAACTGAGCAAAAAGAGCAGATTCGGTACTTTAGTCAACAGGATCTTCGTGAACTTTTCAGTCTCCCACAACAAGGTTTTGATGTTTCCCTTACTCAGAAGCAGCTAAATGAGGAGCATGATCAACAGCACACAATGGAAGAGTCTTTACAAGCCCATATAGAATTCTTGGAAACTCAAGGGATAGCTGGTGTCAGTAACCACAGTTTACTCTTCTCCAAGACAGCACCACCTCTGCCAGAGGTAGACGCGGAGCAGGAGGAAGTTGAAAGGATTAGACAAACTTTACTTGCGAGAAGCTCAACTTCAAGTTCGGCACCTGAATGTAATGTCAATGGGGCTGAATATGCTTTCAAGCCTAAGGATGTAATATTAAATAAGAAGATGCCTTCTTCACCAGCTGATGCAGGAAAAATGACAAAATCAGAGATTAAAGAAAACATCACTAGGCTGTCACAAACTCTTGCAAATAAGGCTTTAGTTTCAAGATTACCTGATAAGGGACAGAAAATACAGAGGCAAATTTCTGAATTGAATGCTGAGCTTCGTAGACTAGACAAGGACGTAATTGACTTGGATGATGTGACTGAGGAGCTTAAAGGAGTGGTGATATAA

Protein Analysis

959

Amino Acids

107.93

Weight (kDa)

6.06

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 261 - 429 1.1e-06 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 264 - 565 7.3e-62 SNF2-related domain
AAA_34 PF13872 280 - 410 4.9e-07 P-loop containing NTP hydrolase pore-1
HDA2-3 PF11496 591 - 740 6.9e-07 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 613 - 722 4.8e-22 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1841
Acc65I GGTACC 1 cut(s) 1970
AccB1I GGYRCC 3 cut(s) 445, 1970, 2581
AccB7I CCANNNNNTGG 1 cut(s) 2143
AccI GTMKAC 2 cut(s) 2511, 2815
AccII CGCG 5 cut(s) 137, 139, 212, 798, 2516
AccIII TCCGGA 1 cut(s) 706
AclWI GGATC 3 cut(s) 2102, 2154, 2316
AcsI RAATTY 2 cut(s) 2429, 2787
AcuI CTGAAG 7 cut(s) 1040, 1052, 1322, 1398, 1514, 1694, 1740
AcyI GRCGYC 1 cut(s) 446
AfiI CCNNNNNNNGG 4 cut(s) 838, 2143, 2339, 2765
AflII CTTAAG 1 cut(s) 1751
AflIII ACRYGT 1 cut(s) 1028
AjnI CCWGG 3 cut(s) 323, 760, 831
Alw21I GWGCWC 2 cut(s) 323, 1458
Alw26I GTCTC 6 cut(s) 149, 485, 743, 1001, 1455, 2333
AlwI GGATC 3 cut(s) 2102, 2154, 2316
Ama87I CYCGRG 1 cut(s) 2122
Aor13HI TCCGGA 1 cut(s) 706
AoxI GGCC 1 cut(s) 1314
ApeKI GCWGC 5 cut(s) 18, 253, 1658, 1715, 2368
ApoI RAATTY 2 cut(s) 2429, 2787
AseI ATTAAT 1 cut(s) 2195
Asp700I GAANNNNTTC 1 cut(s) 1361
Asp718I GGTACC 1 cut(s) 1970
AspLEI GCGC 6 cut(s) 137, 139, 141, 214, 448, 798
AspS9I GGNCC 1 cut(s) 1314
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 5 cut(s) 133, 200, 1361, 1641, 2652
AsuII TTCGAA 2 cut(s) 395, 570
AvaI CYCGRG 1 cut(s) 2122
AxyI CCTNAGG 2 cut(s) 1869, 2625
BaeI ACNNNNGTAYC 2 cut(s) 2284, 2317
BanI GGYRCC 3 cut(s) 445, 1970, 2581
BanII GRGCYC 4 cut(s) 323, 823, 1458, 2032
BarI GAAGNNNNNNTAC 2 cut(s) 2397, 2429
BbsI GAAGAC 1 cut(s) 623
Bbv12I GWGCWC 2 cut(s) 323, 1458
BbvI GCAGC 5 cut(s) 30, 265, 1645, 1702, 2380
BccI CCATC 4 cut(s) 710, 1148, 1637, 1961
BceAI ACGGC 1 cut(s) 126
BciT130I CCWGG 3 cut(s) 325, 762, 833
BclI TGATCA 3 cut(s) 181, 1687, 2386
BcnI CCSGG 1 cut(s) 226
BcoDI GTCTC 6 cut(s) 149, 485, 743, 1001, 1455, 2333
BfaI CTAG 6 cut(s) 1244, 1730, 1784, 1902, 2712, 2819
BfoI RGCGCY 1 cut(s) 449
BfrI CTTAAG 1 cut(s) 1751
BisI GCNGC 9 cut(s) 19, 248, 251, 254, 257, 444, 1659, 1716, 2369
BlpI GCTNAGC 1 cut(s) 2805
BlsI GCNGC 9 cut(s) 20, 249, 252, 255, 258, 445, 1660, 1717, 2370
BmcAI AGTACT 2 cut(s) 665, 2128
Bme1390I CCNGG 4 cut(s) 226, 325, 762, 833
BmeT110I CYCGRG 1 cut(s) 2122
BmgT120I GGNCC 1 cut(s) 1314
BmiI GGNNCC 8 cut(s) 358, 447, 948, 1438, 1972, 2031, 2120, 2583
BmrFI CCNGG 4 cut(s) 226, 325, 762, 833
BmrI ACTGGG 3 cut(s) 210, 1212, 1355
BmsI GCATC 5 cut(s) 799, 1753, 1899, 2640, 2659
BmuI ACTGGG 3 cut(s) 210, 1212, 1355
BpiI GAAGAC 1 cut(s) 623
BpmI CTGGAG 1 cut(s) 307
Bpu10I CCTNAGC 1 cut(s) 1338
Bpu1102I GCTNAGC 1 cut(s) 2805
Bpu14I TTCGAA 2 cut(s) 395, 570
BpuEI CTTGAG 5 cut(s) 87, 337, 350, 2037, 2427
BpuMI CCSGG 1 cut(s) 226
BsaHI GRCGYC 1 cut(s) 446
BsaI GGTCTC 4 cut(s) 485, 743, 1001, 1455
BsaJI CCNNGG 3 cut(s) 761, 832, 1440
BsaWI WCCGGW 2 cut(s) 95, 706
BsaXI ACNNNNNCTCC 4 cut(s) 305, 335, 628, 658
Bsc4I CCNNNNNNNGG 4 cut(s) 838, 2143, 2339, 2765
Bse1I ACTGG 6 cut(s) 216, 495, 508, 1218, 1350, 1364
Bse21I CCTNAGG 2 cut(s) 1869, 2625
Bse3DI GCAATG 4 cut(s) 826, 1286, 1745, 1794
BseAI TCCGGA 1 cut(s) 706
BseBI CCWGG 3 cut(s) 325, 762, 833
BseDI CCNNGG 3 cut(s) 761, 832, 1440
BseGI GGATG 6 cut(s) 538, 718, 1690, 2182, 2635, 2848
BseLI CCNNNNNNNGG 4 cut(s) 838, 2143, 2339, 2765
BseMI GCAATG 4 cut(s) 826, 1286, 1745, 1794
BseNI ACTGG 6 cut(s) 216, 495, 508, 1218, 1350, 1364
BsePI GCGCGC 2 cut(s) 135, 137
BseRI GAGGAG 5 cut(s) 50, 72, 277, 2393, 2870
BseXI GCAGC 5 cut(s) 30, 265, 1645, 1702, 2380
BsgI GTGCAG 1 cut(s) 1644
Bsh1236I CGCG 5 cut(s) 137, 139, 212, 798, 2516
BshFI GGCC 1 cut(s) 1316
BshNI GGYRCC 3 cut(s) 445, 1970, 2581
BsiHKAI GWGCWC 2 cut(s) 323, 1458
BsiHKCI CYCGRG 1 cut(s) 2122
BsiSI CCGG 3 cut(s) 96, 225, 707
BslFI GGGAC 2 cut(s) 1039, 2781
BslI CCNNNNNNNGG 4 cut(s) 838, 2143, 2339, 2765
BsmAI GTCTC 6 cut(s) 149, 485, 743, 1001, 1455, 2333
BsmBI CGTCTC 1 cut(s) 149
BsmFI GGGAC 2 cut(s) 1039, 2781
BsmI GAATGC 3 cut(s) 445, 1080, 2806
BsnI GGCC 1 cut(s) 1316
Bso31I GGTCTC 4 cut(s) 485, 743, 1001, 1455
BsoBI CYCGRG 1 cut(s) 2122
Bsp119I TTCGAA 2 cut(s) 395, 570
Bsp1286I GDGCHC 4 cut(s) 323, 823, 1458, 2032
Bsp13I TCCGGA 1 cut(s) 706
Bsp1407I TGTACA 1 cut(s) 1031
Bsp143I GATC 9 cut(s) 181, 406, 1557, 1687, 2089, 2107, 2146, 2308, 2386
Bsp1720I GCTNAGC 1 cut(s) 2805
BspANI GGCC 1 cut(s) 1316
BspEI TCCGGA 1 cut(s) 706
BspFNI CGCG 5 cut(s) 137, 139, 212, 798, 2516
BspLI GGNNCC 8 cut(s) 358, 447, 948, 1438, 1972, 2031, 2120, 2583
BspPI GGATC 3 cut(s) 2102, 2154, 2316
BspQI GCTCTTC 1 cut(s) 8
BspT104I TTCGAA 2 cut(s) 395, 570
BspT107I GGYRCC 3 cut(s) 445, 1970, 2581
BspTI CTTAAG 1 cut(s) 1751
BspTNI GGTCTC 4 cut(s) 485, 743, 1001, 1455
BsrDI GCAATG 4 cut(s) 826, 1286, 1745, 1794
BsrGI TGTACA 1 cut(s) 1031
BsrI ACTGG 6 cut(s) 216, 495, 508, 1218, 1350, 1364
BssECI CCNNGG 3 cut(s) 761, 832, 1440
BssHII GCGCGC 2 cut(s) 135, 137
BssMI GATC 9 cut(s) 181, 406, 1557, 1687, 2089, 2107, 2146, 2308, 2386
BssNI GRCGYC 1 cut(s) 446
Bst2UI CCWGG 3 cut(s) 325, 762, 833
Bst4CI ACNGT 5 cut(s) 47, 1444, 1466, 2212, 2471
Bst6I CTCTTC 6 cut(s) 8, 537, 567, 1728, 2400, 2483
BstACI GRCGYC 1 cut(s) 446
BstAFI CTTAAG 1 cut(s) 1751
BstAUI TGTACA 1 cut(s) 1031
BstBI TTCGAA 2 cut(s) 395, 570
BstC8I GCNNGC 4 cut(s) 137, 139, 1283, 1713
BstDSI CCRYGG 1 cut(s) 1440
BstEII GGTNACC 1 cut(s) 1647
BstF5I GGATG 6 cut(s) 538, 718, 1690, 2182, 2635, 2848
BstFNI CGCG 5 cut(s) 137, 139, 212, 798, 2516
BstH2I RGCGCY 1 cut(s) 449
BstHHI GCGC 6 cut(s) 137, 139, 141, 214, 448, 798
BstKTI GATC 9 cut(s) 184, 409, 1560, 1690, 2092, 2110, 2149, 2311, 2389
BstMAI GTCTC 6 cut(s) 149, 485, 743, 1001, 1455, 2333
BstMBI GATC 9 cut(s) 181, 406, 1557, 1687, 2089, 2107, 2146, 2308, 2386
BstMWI GCNNNNNNNGC 6 cut(s) 253, 256, 1772, 2281, 2612, 2738
BstNI CCWGG 3 cut(s) 325, 762, 833
BstNSI RCATGY 1 cut(s) 1032
BstPI GGTNACC 1 cut(s) 1647
BstSCI CCNGG 4 cut(s) 224, 323, 760, 831
BstUI CGCG 5 cut(s) 137, 139, 212, 798, 2516
BstV1I GCAGC 5 cut(s) 30, 265, 1645, 1702, 2380
BstV2I GAAGAC 1 cut(s) 623
BstX2I RGATCY 1 cut(s) 2308
BstYI RGATCY 1 cut(s) 2308
Bsu36I CCTNAGG 2 cut(s) 1869, 2625
BsuRI GGCC 1 cut(s) 1316
BtgI CCRYGG 1 cut(s) 1440
BtgZI GCGATG 1 cut(s) 1247
BtsCI GGATG 6 cut(s) 538, 718, 1690, 2182, 2635, 2848
BtsIMutI CAGTG 1 cut(s) 1931
Cac8I GCNNGC 4 cut(s) 137, 139, 1283, 1713
CfoI GCGC 6 cut(s) 137, 139, 141, 214, 448, 798
Cfr13I GGNCC 1 cut(s) 1314
CseI GACGC 3 cut(s) 218, 1475, 2522
CspCI CAANNNNNGTGG 2 cut(s) 1683, 1718
CviAII CATG 5 cut(s) 185, 862, 1029, 1811, 2384
DinI GGCGCC 1 cut(s) 447
DpnI GATC 9 cut(s) 183, 408, 1559, 1689, 2091, 2109, 2148, 2310, 2388
DpnII GATC 9 cut(s) 181, 406, 1557, 1687, 2089, 2107, 2146, 2308, 2386
DraI TTTAAA 1 cut(s) 1614
Eam1104I CTCTTC 6 cut(s) 8, 537, 567, 1728, 2400, 2483
EarI CTCTTC 6 cut(s) 8, 537, 567, 1728, 2400, 2483
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 321, 1456
Eco24I GRGCYC 4 cut(s) 323, 823, 1458, 2032
Eco31I GGTCTC 4 cut(s) 485, 743, 1001, 1455
Eco32I GATATC 1 cut(s) 1938
Eco53kI GAGCTC 2 cut(s) 321, 1456
Eco57I CTGAAG 7 cut(s) 1040, 1052, 1322, 1398, 1514, 1694, 1740
Eco81I CCTNAGG 2 cut(s) 1869, 2625
Eco88I CYCGRG 1 cut(s) 2122
Eco91I GGTNACC 1 cut(s) 1647
EcoICRI GAGCTC 2 cut(s) 321, 1456
EcoO65I GGTNACC 1 cut(s) 1647
EcoRI GAATTC 1 cut(s) 2429
EcoRII CCWGG 3 cut(s) 323, 760, 831
EcoRV GATATC 1 cut(s) 1938
EcoT22I ATGCAT 1 cut(s) 1794
EcoT38I GRGCYC 4 cut(s) 323, 823, 1458, 2032
EgeI GGCGCC 1 cut(s) 447
EheI GGCGCC 1 cut(s) 447
Esp3I CGTCTC 1 cut(s) 149
FaeI CATG 5 cut(s) 188, 865, 1032, 1814, 2387
FalI AAGNNNNNCTT 4 cut(s) 1068, 1100, 2554, 2586
FaqI GGGAC 2 cut(s) 1039, 2781
FatI CATG 5 cut(s) 184, 861, 1028, 1810, 2383
FauI CCCGC 1 cut(s) 633
FbaI TGATCA 3 cut(s) 181, 1687, 2386
FblI GTMKAC 2 cut(s) 2511, 2815
Fnu4HI GCNGC 9 cut(s) 19, 248, 251, 254, 257, 444, 1659, 1716, 2369
FokI GGATG 6 cut(s) 545, 725, 1677, 2189, 2642, 2855
FriOI GRGCYC 4 cut(s) 323, 823, 1458, 2032
Fsp4HI GCNGC 9 cut(s) 19, 248, 251, 254, 257, 444, 1659, 1716, 2369
FspBI CTAG 6 cut(s) 1244, 1730, 1784, 1902, 2712, 2819
GlaI GCGC 6 cut(s) 136, 138, 140, 213, 447, 797
GluI GCNGC 9 cut(s) 19, 248, 251, 254, 257, 444, 1659, 1716, 2369
GsuI CTGGAG 1 cut(s) 307
HaeII RGCGCY 1 cut(s) 449
HaeIII GGCC 1 cut(s) 1316
HapII CCGG 3 cut(s) 96, 225, 707
HgaI GACGC 3 cut(s) 218, 1475, 2522
HhaI GCGC 6 cut(s) 137, 139, 141, 214, 448, 798
Hin1I GRCGYC 1 cut(s) 446
Hin1II CATG 5 cut(s) 188, 865, 1032, 1814, 2387
Hin6I GCGC 6 cut(s) 135, 137, 139, 212, 446, 796
HinP1I GCGC 6 cut(s) 135, 137, 139, 212, 446, 796
HincII GTYRAC 2 cut(s) 1114, 2303
HindII GTYRAC 2 cut(s) 1114, 2303
HpaII CCGG 3 cut(s) 96, 225, 707
HphI GGTGA 5 cut(s) 133, 200, 1361, 1641, 2652
Hpy166II GTNNAC 8 cut(s) 1114, 1223, 1259, 2303, 2318, 2474, 2512, 2816
Hpy188III TCNNGA 9 cut(s) 346, 707, 1070, 1148, 1868, 1925, 2044, 2315, 2751
Hpy8I GTNNAC 8 cut(s) 1114, 1223, 1259, 2303, 2318, 2474, 2512, 2816
Hpy99I CGWCG 2 cut(s) 212, 1491
HpyAV CCTTC 8 cut(s) 125, 346, 391, 459, 794, 1297, 2012, 2664
HpyCH4III ACNGT 5 cut(s) 47, 1444, 1466, 2212, 2471
HpyCH4IV ACGT 2 cut(s) 687, 2829
HpyF10VI GCNNNNNNNGC 6 cut(s) 253, 256, 1772, 2281, 2612, 2738
HpySE526I ACGT 2 cut(s) 687, 2829
Hsp92I GRCGYC 1 cut(s) 446
Hsp92II CATG 5 cut(s) 188, 865, 1032, 1814, 2387
HspAI GCGC 6 cut(s) 135, 137, 139, 212, 446, 796
KasI GGCGCC 1 cut(s) 445
Kpn2I TCCGGA 1 cut(s) 706
KpnI GGTACC 1 cut(s) 1974
Ksp22I TGATCA 3 cut(s) 181, 1687, 2386
Kzo9I GATC 9 cut(s) 181, 406, 1557, 1687, 2089, 2107, 2146, 2308, 2386
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 9 cut(s) 85, 326, 356, 952, 1453, 2035, 2380, 2518, 2857
Lsp1109I GCAGC 5 cut(s) 30, 265, 1645, 1702, 2380
LweI GCATC 5 cut(s) 799, 1753, 1899, 2640, 2659
MaeI CTAG 6 cut(s) 1244, 1730, 1784, 1902, 2712, 2819
MaeII ACGT 2 cut(s) 687, 2829
MaeIII GTNAC 8 cut(s) 47, 1136, 1380, 1647, 1946, 2462, 2718, 2848
MalI GATC 9 cut(s) 183, 408, 1559, 1689, 2091, 2109, 2148, 2310, 2388
MboI GATC 9 cut(s) 181, 406, 1557, 1687, 2089, 2107, 2146, 2308, 2386
MflI RGATCY 1 cut(s) 2308
MhlI GDGCHC 4 cut(s) 323, 823, 1458, 2032
MluCI AATT 8 cut(s) 755, 980, 1992, 2160, 2429, 2787, 2795, 2832
Mly113I GGCGCC 1 cut(s) 446
MlyI GAGTC 4 cut(s) 251, 1119, 1388, 2417
MmeI TCCRAC 4 cut(s) 253, 1719, 1833, 1981
Mph1103I ATGCAT 1 cut(s) 1794
MroI TCCGGA 1 cut(s) 706
MroXI GAANNNNTTC 1 cut(s) 1361
MslI CAYNNNNRTG 1 cut(s) 2587
MspA1I CMGCKG 5 cut(s) 989, 1584, 1591, 1944, 2666
MspCI CTTAAG 1 cut(s) 1751
MspI CCGG 3 cut(s) 96, 225, 707
MspR9I CCNGG 4 cut(s) 226, 325, 762, 833
Mva1269I GAATGC 3 cut(s) 445, 1080, 2806
MvaI CCWGG 3 cut(s) 325, 762, 833
MvnI CGCG 5 cut(s) 137, 139, 212, 798, 2516
MwoI GCNNNNNNNGC 6 cut(s) 253, 256, 1772, 2281, 2612, 2738
NarI GGCGCC 1 cut(s) 446
NciI CCSGG 1 cut(s) 226
NdeII GATC 9 cut(s) 181, 406, 1557, 1687, 2089, 2107, 2146, 2308, 2386
NlaIII CATG 5 cut(s) 188, 865, 1032, 1814, 2387
NlaIV GGNNCC 8 cut(s) 358, 447, 948, 1438, 1972, 2031, 2120, 2583
NmuCI GTSAC 6 cut(s) 47, 1136, 1380, 1647, 2718, 2848
NsiI ATGCAT 1 cut(s) 1794
NspI RCATGY 1 cut(s) 1032
NspV TTCGAA 2 cut(s) 395, 570
PauI GCGCGC 2 cut(s) 135, 137
PciI ACATGT 1 cut(s) 1028
PciSI GCTCTTC 1 cut(s) 8
PcsI WCGNNNNNNNCGW 3 cut(s) 392, 1390, 2155
PctI GAATGC 3 cut(s) 445, 1080, 2806
PdmI GAANNNNTTC 1 cut(s) 1361
PflMI CCANNNNNTGG 1 cut(s) 2143
PfoI TCCNGGA 1 cut(s) 224
PkrI GCNGC 9 cut(s) 20, 249, 252, 255, 258, 445, 1660, 1717, 2370
PleI GAGTC 4 cut(s) 250, 1118, 1387, 2416
PluTI GGCGCC 1 cut(s) 449
PpsI GAGTC 4 cut(s) 250, 1118, 1387, 2416
PscI ACATGT 1 cut(s) 1028
PshBI ATTAAT 1 cut(s) 2195
PsiI TTATAA 1 cut(s) 1841
Psp124BI GAGCTC 2 cut(s) 323, 1458
Psp6I CCWGG 3 cut(s) 323, 760, 831
PspEI GGTNACC 1 cut(s) 1647
PspGI CCWGG 3 cut(s) 323, 760, 831
PspN4I GGNNCC 8 cut(s) 358, 447, 948, 1438, 1972, 2031, 2120, 2583
PspPI GGNCC 1 cut(s) 1314
PsuI RGATCY 1 cut(s) 2308
PteI GCGCGC 2 cut(s) 135, 137
PvuII CAGCTG 3 cut(s) 989, 1584, 2666
RseI CAYNNNNRTG 1 cut(s) 2587
SacI GAGCTC 2 cut(s) 323, 1458
SapI GCTCTTC 1 cut(s) 8
SatI GCNGC 9 cut(s) 19, 248, 251, 254, 257, 444, 1659, 1716, 2369
Sau3AI GATC 9 cut(s) 181, 406, 1557, 1687, 2089, 2107, 2146, 2308, 2386
Sau96I GGNCC 1 cut(s) 1314
ScaI AGTACT 2 cut(s) 665, 2128
SchI GAGTC 4 cut(s) 251, 1119, 1388, 2417
ScrFI CCNGG 4 cut(s) 226, 325, 762, 833
SduI GDGCHC 4 cut(s) 323, 823, 1458, 2032
SfaNI GCATC 5 cut(s) 799, 1753, 1899, 2640, 2659
SfoI GGCGCC 1 cut(s) 447
SfuI TTCGAA 2 cut(s) 395, 570
SmiMI CAYNNNNRTG 1 cut(s) 2587
SmlI CTYRAG 6 cut(s) 102, 316, 329, 1751, 2052, 2442
SmoI CTYRAG 6 cut(s) 102, 316, 329, 1751, 2052, 2442
Sse9I AATT 8 cut(s) 755, 980, 1992, 2160, 2429, 2787, 2795, 2832
SspDI GGCGCC 1 cut(s) 445
SspI AATATT 1 cut(s) 2637
SspMI CTAG 6 cut(s) 1244, 1730, 1784, 1902, 2712, 2819
SstI GAGCTC 2 cut(s) 323, 1458
StyD4I CCNGG 4 cut(s) 224, 323, 760, 831
TaaI ACNGT 5 cut(s) 47, 1444, 1466, 2212, 2471
TaiI ACGT 2 cut(s) 690, 2832
TaqI TCGA 8 cut(s) 157, 345, 395, 458, 570, 1149, 1377, 2158
TasI AATT 8 cut(s) 755, 980, 1992, 2160, 2429, 2787, 2795, 2832
TatI WGTACW 7 cut(s) 432, 663, 1031, 1063, 1220, 1726, 2126
TauI GCSGC 4 cut(s) 250, 253, 259, 446
TscAI CASTG 1 cut(s) 1938
TseFI GTSAC 6 cut(s) 47, 1136, 1380, 1647, 2718, 2848
TseI GCWGC 5 cut(s) 18, 253, 1658, 1715, 2368
Tsp45I GTSAC 6 cut(s) 47, 1136, 1380, 1647, 2718, 2848
TspDTI ATGAA 5 cut(s) 549, 555, 902, 1617, 1734
TspGWI ACGGA 1 cut(s) 635
TspRI CASTG 1 cut(s) 1938
Van91I CCANNNNNTGG 1 cut(s) 2143
Vha464I CTTAAG 1 cut(s) 1751
VspI ATTAAT 1 cut(s) 2195
XapI RAATTY 2 cut(s) 2429, 2787
XceI RCATGY 1 cut(s) 1032
XcmI CCANNNNNNNNNTGG 2 cut(s) 1964, 2021
XmiI GTMKAC 2 cut(s) 2511, 2815
XmnI GAANNNNTTC 1 cut(s) 1361
XspI CTAG 6 cut(s) 1244, 1730, 1784, 1902, 2712, 2819
ZrmI AGTACT 2 cut(s) 665, 2128
Zsp2I ATGCAT 1 cut(s) 1794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.