Rroxscaffold_3G00237800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008163
Physical Location & Seq
Reverse (-)
25504130 .. 25505030
901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00237800.1

Sequence Viewer

Length: 462 bp
ATGGAATCGACGAACGAAACAGCTGCAGGCGGAGGGTCCGAGGGCGGAGAAGCATGCTTGAAACGAAATCCGAGTTTGATCGCTTCGGGGTTGGAGTTGAAGGGCGCCGGAGATAGACTTCGCCCCGTTCGTCGGGGACCGAGACCCGATGAACCCCGGCGGAAGGTCAACGGAGCGGAACCTCCGGTGGCCGACGACGGAGATGGACCAGTCCCGAGCTCGGCTACTCTAATTGCAGTCGCTCGATTTGAAAGAATGGGAATTATGGGTCCGGTGCTCTTCGGTTATAGAAGCTTGGCAAATGTTGAGGACACCCAACAAGGAGGGAGAAACTATTGGTTCAGCTTAATTGGTATGGGAGATTGGTGTCCAAAACCATTGTTGGAGTTGGATTGCAGCTTGGATGGAATTGGTTCCCGAGTTGGAGAAGAAGAGATGGGTAATGCTCCTTTGAGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.15

Weight (kDa)

4.85

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 104
AccBSI CCGCTC 1 cut(s) 176
AciI CCGC 4 cut(s) 30, 45, 160, 176
AcoI YGGCCR 1 cut(s) 189
AcyI GRCGYC 1 cut(s) 105
AfiI CCNNNNNNNGG 3 cut(s) 132, 163, 220
AgsI TTSAA 3 cut(s) 61, 100, 251
AluBI AGCT 5 cut(s) 23, 219, 294, 345, 399
AluI AGCT 5 cut(s) 23, 219, 294, 345, 399
Alw21I GWGCWC 2 cut(s) 221, 279
Alw26I GTCTC 1 cut(s) 136
Ama87I CYCGRG 2 cut(s) 214, 417
AoxI GGCC 1 cut(s) 189
ApeKI GCWGC 2 cut(s) 23, 396
Asp700I GAANNNNTTC 1 cut(s) 412
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 4 cut(s) 36, 137, 206, 269
AsuC2I CCSGG 1 cut(s) 157
AvaI CYCGRG 2 cut(s) 214, 417
AvaII GGWCC 4 cut(s) 36, 137, 206, 269
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 1 cut(s) 221
Bbv12I GWGCWC 2 cut(s) 221, 279
BbvI GCAGC 2 cut(s) 10, 408
BccI CCATC 3 cut(s) 197, 398, 430
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 1 cut(s) 136
BfmI CTRYAG 1 cut(s) 24
BfoI RGCGCY 1 cut(s) 108
BisI GCNGC 2 cut(s) 24, 397
BlsI GCNGC 2 cut(s) 25, 398
Bme1390I CCNGG 1 cut(s) 157
Bme18I GGWCC 4 cut(s) 36, 137, 206, 269
BmeT110I CYCGRG 2 cut(s) 214, 417
BmgT120I GGNCC 4 cut(s) 36, 137, 206, 269
BmiI GGNNCC 6 cut(s) 37, 106, 138, 180, 270, 415
BmrFI CCNGG 1 cut(s) 157
BpuMI CCSGG 1 cut(s) 157
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 2 cut(s) 39, 155
BsaWI WCCGGW 2 cut(s) 184, 271
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 163, 220
Bse1I ACTGG 1 cut(s) 209
BseDI CCNNGG 2 cut(s) 39, 155
BseGI GGATG 1 cut(s) 409
BseLI CCNNNNNNNGG 3 cut(s) 132, 163, 220
BseNI ACTGG 1 cut(s) 209
BseXI GCAGC 2 cut(s) 10, 408
BshFI GGCC 1 cut(s) 191
BshNI GGYRCC 1 cut(s) 104
BsiHKAI GWGCWC 2 cut(s) 221, 279
BsiHKCI CYCGRG 2 cut(s) 214, 417
BsiSI CCGG 4 cut(s) 108, 157, 185, 272
BslFI GGGAC 2 cut(s) 150, 197
BslI CCNNNNNNNGG 3 cut(s) 132, 163, 220
BsmAI GTCTC 1 cut(s) 136
BsmFI GGGAC 2 cut(s) 150, 197
BsnI GGCC 1 cut(s) 191
Bso31I GGTCTC 1 cut(s) 136
BsoBI CYCGRG 2 cut(s) 214, 417
Bsp1286I GDGCHC 2 cut(s) 221, 279
Bsp143I GATC 1 cut(s) 78
BspACI CCGC 4 cut(s) 30, 45, 160, 176
BspANI GGCC 1 cut(s) 191
BspLI GGNNCC 6 cut(s) 37, 106, 138, 180, 270, 415
BspMAI CTGCAG 1 cut(s) 28
BspQI GCTCTTC 1 cut(s) 284
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 136
BsrBI CCGCTC 1 cut(s) 176
BsrI ACTGG 1 cut(s) 209
BssECI CCNNGG 2 cut(s) 39, 155
BssMI GATC 1 cut(s) 78
BssNI GRCGYC 1 cut(s) 105
Bst6I CTCTTC 2 cut(s) 284, 426
BstACI GRCGYC 1 cut(s) 105
BstC8I GCNNGC 2 cut(s) 28, 55
BstF5I GGATG 1 cut(s) 409
BstH2I RGCGCY 1 cut(s) 108
BstHHI GCGC 1 cut(s) 107
BstKTI GATC 1 cut(s) 81
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 1 cut(s) 78
BstNSI RCATGY 1 cut(s) 57
BstSCI CCNGG 1 cut(s) 155
BstSFI CTRYAG 1 cut(s) 24
BstV1I GCAGC 2 cut(s) 10, 408
BsuRI GGCC 1 cut(s) 191
BtsCI GGATG 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 28, 55
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 4 cut(s) 36, 137, 206, 269
CviAII CATG 1 cut(s) 54
CviJI RGCY 7 cut(s) 23, 191, 219, 224, 294, 345, 399
CviKI_1 RGCY 7 cut(s) 23, 191, 219, 224, 294, 345, 399
DinI GGCGCC 1 cut(s) 106
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
EaeI YGGCCR 1 cut(s) 189
Eam1104I CTCTTC 2 cut(s) 284, 426
EarI CTCTTC 2 cut(s) 284, 426
EciI GGCGGA 3 cut(s) 45, 60, 175
Ecl136II GAGCTC 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 136
Eco47I GGWCC 4 cut(s) 36, 137, 206, 269
Eco53kI GAGCTC 1 cut(s) 219
Eco88I CYCGRG 2 cut(s) 214, 417
EcoICRI GAGCTC 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 221
EgeI GGCGCC 1 cut(s) 106
EheI GGCGCC 1 cut(s) 106
FaeI CATG 1 cut(s) 57
FaiI YATR 4 cut(s) 55, 266, 288, 356
FaqI GGGAC 2 cut(s) 150, 197
FatI CATG 1 cut(s) 53
Fnu4HI GCNGC 2 cut(s) 24, 397
FokI GGATG 1 cut(s) 416
FriOI GRGCYC 1 cut(s) 221
Fsp4HI GCNGC 2 cut(s) 24, 397
GlaI GCGC 1 cut(s) 106
GluI GCNGC 2 cut(s) 24, 397
HaeII RGCGCY 1 cut(s) 108
HaeIII GGCC 1 cut(s) 191
HapII CCGG 4 cut(s) 108, 157, 185, 272
HhaI GCGC 1 cut(s) 107
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 1 cut(s) 57
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 292
HinfI GANTC 1 cut(s) 5
HpaII CCGG 4 cut(s) 108, 157, 185, 272
Hpy166II GTNNAC 1 cut(s) 169
Hpy188I TCNGA 2 cut(s) 40, 72
Hpy188III TCNNGA 2 cut(s) 214, 417
Hpy8I GTNNAC 1 cut(s) 169
Hpy99I CGWCG 4 cut(s) 13, 135, 197, 200
HpyAV CCTTC 2 cut(s) 94, 157
HpyCH4V TGCA 3 cut(s) 26, 236, 396
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 1 cut(s) 57
HspAI GCGC 1 cut(s) 105
KasI GGCGCC 1 cut(s) 104
Kzo9I GATC 1 cut(s) 78
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 2 cut(s) 173, 451
LpnPI CCDG 6 cut(s) 12, 121, 170, 198, 222, 285
Lsp1109I GCAGC 2 cut(s) 10, 408
MalI GATC 1 cut(s) 80
MbiI CCGCTC 1 cut(s) 176
MboI GATC 1 cut(s) 78
MboII GAAGA 3 cut(s) 271, 440, 443
MhlI GDGCHC 2 cut(s) 221, 279
MluCI AATT 4 cut(s) 231, 261, 348, 408
Mly113I GGCGCC 1 cut(s) 105
MmeI TCCRAC 4 cut(s) 72, 363, 369, 403
MnlI CCTC 5 cut(s) 26, 34, 192, 301, 317
MroXI GAANNNNTTC 1 cut(s) 412
MseI TTAA 1 cut(s) 347
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 4 cut(s) 108, 157, 185, 272
MspR9I CCNGG 1 cut(s) 157
NarI GGCGCC 1 cut(s) 105
NciI CCSGG 1 cut(s) 157
NdeII GATC 1 cut(s) 78
NlaIII CATG 1 cut(s) 57
NlaIV GGNNCC 6 cut(s) 37, 106, 138, 180, 270, 415
NmeAIII GCCGAG 1 cut(s) 200
NspI RCATGY 1 cut(s) 57
PaeI GCATGC 1 cut(s) 57
PciSI GCTCTTC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 127
PdmI GAANNNNTTC 1 cut(s) 412
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 2 cut(s) 25, 398
PluTI GGCGCC 1 cut(s) 108
Psp124BI GAGCTC 1 cut(s) 221
PspN4I GGNNCC 6 cut(s) 37, 106, 138, 180, 270, 415
PspPI GGNCC 4 cut(s) 36, 137, 206, 269
PstI CTGCAG 1 cut(s) 28
PvuII CAGCTG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 221
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 1 cut(s) 347
SatI GCNGC 2 cut(s) 24, 397
Sau3AI GATC 1 cut(s) 78
Sau96I GGNCC 4 cut(s) 36, 137, 206, 269
ScrFI CCNGG 1 cut(s) 157
SduI GDGCHC 2 cut(s) 221, 279
SetI ASST 7 cut(s) 25, 168, 184, 221, 296, 347, 401
SfcI CTRYAG 1 cut(s) 24
SfoI GGCGCC 1 cut(s) 106
SinI GGWCC 4 cut(s) 36, 137, 206, 269
SphI GCATGC 1 cut(s) 57
Sse9I AATT 4 cut(s) 231, 261, 348, 408
SsiI CCGC 4 cut(s) 30, 45, 160, 176
SspDI GGCGCC 1 cut(s) 104
SstI GAGCTC 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 155
TaqI TCGA 2 cut(s) 8, 244
TaqII GACCGA 1 cut(s) 154
TasI AATT 4 cut(s) 231, 261, 348, 408
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 347
Tru9I TTAA 1 cut(s) 347
TseI GCWGC 2 cut(s) 23, 396
TspDTI ATGAA 1 cut(s) 165
TspGWI ACGGA 2 cut(s) 186, 213
VpaK11BI GGWCC 4 cut(s) 36, 137, 206, 269
XceI RCATGY 1 cut(s) 57
XmnI GAANNNNTTC 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.