Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0008163

Physical Location & Seq

Genomic Coordinates Reverse (-)

30057865 .. 30064504

Gene Structure 6640 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00352780.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 717 bp

ATGAAGTTCCGGATTGGATGTGCGTTGTTTGTTGTGTTGTGCCTTTGTTGTTCGGGAGCGAGTCCCGGTGCAGCAGTAGCAAAAGCTGCGATGCTTTGGGTTCGAGTCGGTTTTTGGGTCGACTTCTTCGGATTATACGCCAAAGAAGGAACGGTGAGGATGGTCGGGGTGTACGGAATACTCGGGAAGGAACAAGAGATGTGGGAGACTACCGATAAGAGCTTGCGGGATGCTTTCCGGGACTTGAATGCTCTCATGAGTAAAGCTAAAGAGATGGTGATGCTAGCGAGAGAAAATGAGGCAGAAACTTTTATCCGGCTCGACTTCTCGGCTGGTGCGGCGAGAGGACGAGGAATTGGGTTCCAAACAAGACATGCAAGGCGGTTACCGAGTTGTTTGAGGAACAGAATTGATCTCACCGGAGGATCTGCTGCAAGCATGTTTTCTTTGGGAGAAGTTGATGTCCCGTGTCCAAAACCATTGTTGGAGTTGAATTGCAGCTTGGATGGAATTGGTTCCCGAGTTGGAGAAGAAGAGATGGTTGTCCTTGATGACCTACCTGTTCCTAGTTCTAATTTAACAGATGTGCGTGGTGTCCATGTTACCCGGGTTGTGGGTAGGGAGGGTGCTACAGCTATCGACTCGGCGACCACCGTCGGCCTCGAAGATGCCGAGTTCACCACCATCTCCGCCGGACGAGGAATGGACGACGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000814 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005770 GO:0005886 GO:0006508 GO:0006511 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007033 GO:0007034 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010008 GO:0010154 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0019538 GO:0019941 GO:0022414 GO:0030163 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0032182 GO:0032501 GO:0032502 GO:0032509 GO:0032511 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0036452 GO:0042886 GO:0043130 GO:0043162 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043328 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0045324 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0061458 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071944 GO:0071985 GO:0072594 GO:0072665 GO:0072666 GO:0090351 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

EAP30 Vps36_ESCRT-II

Protein Analysis

238

Amino Acids

25.68

Weight (kDa)

5.25

Isoelectric Point (pI)

27.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EAP30	PF04157	55 - 99	5e-06	EAP30/Vps36 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008163)

Species	Orthologous Gene IDs
pyrus_communis	pycom11g15310 pycom11g15370
rosa_laevigata	RLG00000006965
rosa_multiflora	Rmu_co8253563.1_g000001 Rmu_sc0003371.1_g000003 Rmu_sc0013146.1_g000005 Rmu_sc0013284.1_g000001 Rmu_sc0021708.1_g000005
rosa_roxburghii	Rroxscaffold_1G00027810 Rroxscaffold_1G00042300 Rroxscaffold_1G00042310 Rroxscaffold_3G00237790 Rroxscaffold_3G00237800 Rroxscaffold_4G00285110 Rroxscaffold_5G00345700 Rroxscaffold_5G00345710 Rroxscaffold_5G00352780 Rroxscaffold_7G00192560 Rroxscaffold_7G00204520
rosa_rugosa	Rorug05G0196200
rosa_samantha	Rh6BG433000 Rh6CG482800 Rh7AG241200 Rh7DG247200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	120
AccIII	TCCGGA	1 cut(s)	9
AciI	CCGC	4 cut(s)	226, 338, 382, 690
AclWI	GGATC	1 cut(s)	433
AfaI	GTAC	1 cut(s)	173
AfiI	CCNNNNNNNGG	1 cut(s)	613
AgsI	TTSAA	2 cut(s)	247, 493
AjuI	GAANNNNNNNTTGG	2 cut(s)	485, 517
AluBI	AGCT	5 cut(s)	86, 222, 266, 501, 635
AluI	AGCT	5 cut(s)	86, 222, 266, 501, 635
Alw26I	GTCTC	1 cut(s)	200
AlwI	GGATC	1 cut(s)	433
Ama87I	CYCGRG	3 cut(s)	182, 519, 606
Aor13HI	TCCGGA	1 cut(s)	9
AoxI	GGCC	1 cut(s)	658
ApeKI	GCWGC	4 cut(s)	71, 86, 431, 498
Asp700I	GAANNNNTTC	1 cut(s)	514
AsuC2I	CCSGG	4 cut(s)	66, 239, 607, 608
AsuHPI	GGTGA	4 cut(s)	166, 289, 409, 670
AsuNHI	GCTAGC	1 cut(s)	283
AvaI	CYCGRG	3 cut(s)	182, 519, 606
BbvI	GCAGC	4 cut(s)	73, 83, 418, 510
BccI	CCATC	5 cut(s)	154, 268, 500, 532, 692
BcnI	CCSGG	4 cut(s)	66, 239, 607, 608
BcoDI	GTCTC	1 cut(s)	200
BfaI	CTAG	2 cut(s)	284, 567
BfmI	CTRYAG	1 cut(s)	630
BisI	GCNGC	5 cut(s)	72, 87, 339, 432, 499
BlsI	GCNGC	5 cut(s)	73, 88, 340, 433, 500
Bme1390I	CCNGG	4 cut(s)	66, 239, 607, 608
BmeT110I	CYCGRG	3 cut(s)	182, 519, 606
BmiI	GGNNCC	2 cut(s)	362, 517
BmrFI	CCNGG	4 cut(s)	66, 239, 607, 608
BmsI	GCATC	4 cut(s)	81, 220, 270, 658
BmtI	GCTAGC	1 cut(s)	287
BoxI	GACNNNNGTC	1 cut(s)	653
BpuMI	CCSGG	4 cut(s)	66, 239, 607, 608
BsaJI	CCNNGG	1 cut(s)	606
BsaWI	WCCGGW	2 cut(s)	9, 419
Bsc4I	CCNNNNNNNGG	1 cut(s)	613
BseAI	TCCGGA	1 cut(s)	9
BseDI	CCNNGG	1 cut(s)	606
BseGI	GGATG	4 cut(s)	23, 165, 235, 511
BseLI	CCNNNNNNNGG	1 cut(s)	613
BseXI	GCAGC	4 cut(s)	73, 83, 418, 510
BsgI	GTGCAG	1 cut(s)	90
BshFI	GGCC	1 cut(s)	660
BsiHKCI	CYCGRG	3 cut(s)	182, 519, 606
BsiSI	CCGG	7 cut(s)	10, 66, 238, 316, 420, 607, 693
BslFI	GGGAC	3 cut(s)	48, 254, 449
BslI	CCNNNNNNNGG	1 cut(s)	613
BsmAI	GTCTC	1 cut(s)	200
BsmFI	GGGAC	3 cut(s)	48, 254, 449
BsmI	GAATGC	1 cut(s)	253
BsnI	GGCC	1 cut(s)	660
BsoBI	CYCGRG	3 cut(s)	182, 519, 606
Bsp13I	TCCGGA	1 cut(s)	9
Bsp143I	GATC	2 cut(s)	412, 425
BspACI	CCGC	4 cut(s)	226, 338, 382, 690
BspANI	GGCC	1 cut(s)	660
BspEI	TCCGGA	1 cut(s)	9
BspHI	TCATGA	1 cut(s)	255
BspLI	GGNNCC	2 cut(s)	362, 517
BspOI	GCTAGC	1 cut(s)	287
BspPI	GGATC	1 cut(s)	433
BssECI	CCNNGG	1 cut(s)	606
BssMI	GATC	2 cut(s)	412, 425
Bst4CI	ACNGT	2 cut(s)	154, 655
Bst6I	CTCTTC	1 cut(s)	528
BstAPI	GCANNNNNTGC	1 cut(s)	86
BstC8I	GCNNGC	3 cut(s)	224, 285, 436
BstEII	GGTNACC	1 cut(s)	384
BstF5I	GGATG	4 cut(s)	23, 165, 235, 511
BstKTI	GATC	2 cut(s)	415, 428
BstMAI	GTCTC	1 cut(s)	200
BstMBI	GATC	2 cut(s)	412, 425
BstMWI	GCNNNNNNNGC	3 cut(s)	77, 86, 338
BstNSI	RCATGY	2 cut(s)	377, 442
BstPAI	GACNNNNGTC	1 cut(s)	653
BstPI	GGTNACC	1 cut(s)	384
BstSCI	CCNGG	4 cut(s)	64, 237, 605, 606
BstSFI	CTRYAG	1 cut(s)	630
BstV1I	GCAGC	4 cut(s)	73, 83, 418, 510
BstX2I	RGATCY	1 cut(s)	425
BstYI	RGATCY	1 cut(s)	425
BsuRI	GGCC	1 cut(s)	660
BtgZI	GCGATG	1 cut(s)	104
BtsCI	GGATG	4 cut(s)	23, 165, 235, 511
Cac8I	GCNNGC	3 cut(s)	224, 285, 436
CciI	TCATGA	1 cut(s)	255
Cfr9I	CCCGGG	1 cut(s)	606
Csp6I	GTAC	1 cut(s)	172
CspCI	CAANNNNNGTGG	2 cut(s)	182, 217
CviAII	CATG	4 cut(s)	256, 374, 439, 599
CviJI	RGCY	8 cut(s)	86, 222, 266, 319, 332, 501, 635, 660
CviKI_1	RGCY	8 cut(s)	86, 222, 266, 319, 332, 501, 635, 660
CviQI	GTAC	1 cut(s)	172
DpnI	GATC	2 cut(s)	414, 427
DpnII	GATC	2 cut(s)	412, 425
Eam1104I	CTCTTC	1 cut(s)	528
EarI	CTCTTC	1 cut(s)	528
EciI	GGCGGA	1 cut(s)	679
Eco88I	CYCGRG	3 cut(s)	182, 519, 606
Eco91I	GGTNACC	1 cut(s)	384
EcoO65I	GGTNACC	1 cut(s)	384
FaeI	CATG	4 cut(s)	259, 377, 442, 602
FaiI	YATR	5 cut(s)	136, 257, 375, 440, 600
FaqI	GGGAC	3 cut(s)	48, 254, 449
FatI	CATG	4 cut(s)	255, 373, 438, 598
FauI	CCCGC	1 cut(s)	219
FblI	GTMKAC	1 cut(s)	120
Fnu4HI	GCNGC	5 cut(s)	72, 87, 339, 432, 499
FokI	GGATG	4 cut(s)	30, 172, 242, 518
Fsp4HI	GCNGC	5 cut(s)	72, 87, 339, 432, 499
FspBI	CTAG	2 cut(s)	284, 567
GluI	GCNGC	5 cut(s)	72, 87, 339, 432, 499
HaeIII	GGCC	1 cut(s)	660
HapII	CCGG	7 cut(s)	10, 66, 238, 316, 420, 607, 693
Hin1II	CATG	4 cut(s)	259, 377, 442, 602
HincII	GTYRAC	1 cut(s)	121
HindII	GTYRAC	1 cut(s)	121
HinfI	GANTC	3 cut(s)	61, 105, 641
HpaII	CCGG	7 cut(s)	10, 66, 238, 316, 420, 607, 693
HphI	GGTGA	4 cut(s)	166, 289, 409, 670
Hpy166II	GTNNAC	3 cut(s)	121, 172, 678
Hpy188I	TCNGA	1 cut(s)	131
Hpy188III	TCNNGA	5 cut(s)	10, 54, 184, 256, 519
Hpy8I	GTNNAC	3 cut(s)	121, 172, 678
Hpy99I	CGWCG	2 cut(s)	659, 713
HpyAV	CCTTC	2 cut(s)	140, 181
HpyCH4III	ACNGT	2 cut(s)	154, 655
HpyCH4V	TGCA	4 cut(s)	71, 377, 434, 498
HpyF10VI	GCNNNNNNNGC	3 cut(s)	77, 86, 338
Hsp92II	CATG	4 cut(s)	259, 377, 442, 602
Kpn2I	TCCGGA	1 cut(s)	9
Kzo9I	GATC	2 cut(s)	412, 425
LmnI	GCTCC	1 cut(s)	56
LpnPI	CCDG	9 cut(s)	23, 79, 251, 318, 329, 433, 573, 620, 706
Lsp1109I	GCAGC	4 cut(s)	73, 83, 418, 510
LweI	GCATC	4 cut(s)	81, 220, 270, 658
MaeI	CTAG	2 cut(s)	284, 567
MaeIII	GTNAC	2 cut(s)	384, 601
MalI	GATC	2 cut(s)	414, 427
MboI	GATC	2 cut(s)	412, 425
MboII	GAAGA	4 cut(s)	118, 542, 545, 677
MflI	RGATCY	1 cut(s)	425
MluCI	AATT	5 cut(s)	354, 408, 493, 510, 574
MlyI	GAGTC	3 cut(s)	70, 114, 635
MmeI	TCCRAC	2 cut(s)	465, 505
MnlI	CCTC	9 cut(s)	150, 292, 338, 344, 393, 416, 616, 671, 692
MroI	TCCGGA	1 cut(s)	9
MroXI	GAANNNNTTC	1 cut(s)	514
MseI	TTAA	1 cut(s)	578
MspI	CCGG	7 cut(s)	10, 66, 238, 316, 420, 607, 693
MspR9I	CCNGG	4 cut(s)	66, 239, 607, 608
Mva1269I	GAATGC	1 cut(s)	253
MwoI	GCNNNNNNNGC	3 cut(s)	77, 86, 338
NciI	CCSGG	4 cut(s)	66, 239, 607, 608
NdeII	GATC	2 cut(s)	412, 425
NheI	GCTAGC	1 cut(s)	283
NlaIII	CATG	4 cut(s)	259, 377, 442, 602
NlaIV	GGNNCC	2 cut(s)	362, 517
NmeAIII	GCCGAG	3 cut(s)	308, 623, 697
NspI	RCATGY	2 cut(s)	377, 442
PagI	TCATGA	1 cut(s)	255
PcsI	WCGNNNNNNNCGW	1 cut(s)	669
PctI	GAATGC	1 cut(s)	253
PdmI	GAANNNNTTC	1 cut(s)	514
PfoI	TCCNGGA	1 cut(s)	237
PkrI	GCNGC	5 cut(s)	73, 88, 340, 433, 500
PleI	GAGTC	3 cut(s)	69, 113, 635
PpsI	GAGTC	3 cut(s)	69, 113, 635
PshAI	GACNNNNGTC	1 cut(s)	653
PspEI	GGTNACC	1 cut(s)	384
PspN4I	GGNNCC	2 cut(s)	362, 517
PsuI	RGATCY	1 cut(s)	425
RsaI	GTAC	1 cut(s)	173
RsaNI	GTAC	1 cut(s)	172
SalI	GTCGAC	1 cut(s)	119
SaqAI	TTAA	1 cut(s)	578
SatI	GCNGC	5 cut(s)	72, 87, 339, 432, 499
Sau3AI	GATC	2 cut(s)	412, 425
SchI	GAGTC	3 cut(s)	70, 114, 635
ScrFI	CCNGG	4 cut(s)	66, 239, 607, 608
SetI	ASST	7 cut(s)	88, 224, 268, 503, 558, 562, 637
SfaNI	GCATC	4 cut(s)	81, 220, 270, 658
SfcI	CTRYAG	1 cut(s)	630
SmaI	CCCGGG	1 cut(s)	608
Sse9I	AATT	5 cut(s)	354, 408, 493, 510, 574
SsiI	CCGC	4 cut(s)	226, 338, 382, 690
SspMI	CTAG	2 cut(s)	284, 567
StyD4I	CCNGG	4 cut(s)	64, 237, 605, 606
TaaI	ACNGT	2 cut(s)	154, 655
TaqI	TCGA	5 cut(s)	103, 120, 321, 639, 663
TasI	AATT	5 cut(s)	354, 408, 493, 510, 574
TauI	GCSGC	1 cut(s)	341
Tru1I	TTAA	1 cut(s)	578
Tru9I	TTAA	1 cut(s)	578
TseI	GCWGC	4 cut(s)	71, 86, 431, 498
TspDTI	ATGAA	1 cut(s)	17
TspGWI	ACGGA	1 cut(s)	189
TspMI	CCCGGG	1 cut(s)	606
XceI	RCATGY	2 cut(s)	377, 442
XmaI	CCCGGG	1 cut(s)	606
XmiI	GTMKAC	1 cut(s)	120
XmnI	GAANNNNTTC	1 cut(s)	514
XspI	CTAG	2 cut(s)	284, 567

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.