Rroxscaffold_3G00242200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006403
Physical Location & Seq
Forward (+)
34585776 .. 34588018
2243 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00242200.1

Sequence Viewer

Length: 582 bp
ATGGGCATGGCTTTCCGCAGAGGCAAAGCCAAGTTCACAATTTCTCCGACAACAATCCTACTTGCACAAAAGCACGAGGGTGTATTCACCAAGCTGCCGTCCATGGTGACAGACCCAGCAAAGCGAGATCAATCCCCGTACTACAAGTTCCATAAAGAATACTCGCACCCTACCAATAATTGCCGGCACCCGAGGCAACAATTCGAGGATCCGATGGACCATGGAAAACTCCTTCAATACAAAATCCATAGTGGAGAAAGAAGGAGACAAAGTCTTGAAAAGGGTCGTGGAAAAACTAAAGACGGAGAGACGGAAAAATTTAATCCAACATGGGAAGGCCCCTACGAAGTCTTAAAAGTGTTTGGCAACCGCGGAAATAGCGATTGGAAGAACATTGTCGTGCTCGTGAAACGCAATTCACATGCGGAAGTACTATGCTTGACAATGATGCGACCAAAAGGCAGCCGTCGCAAGCATCAAGAAGGTGAAATACAATATCCTCAAGGAGGAGAGAATTCCCTCGAGGTAGGTTTAGTCGAGGTGAGACACAATCCCCTCAAGGTGGATATGGGCATAGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

22.24

Weight (kDa)

9.77

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 521
AccB1I GGYRCC 1 cut(s) 186
AccII CGCG 1 cut(s) 372
AciI CCGC 4 cut(s) 16, 370, 372, 425
AclWI GGATC 2 cut(s) 203, 216
AcsI RAATTY 2 cut(s) 317, 514
AfaI GTAC 2 cut(s) 140, 432
AfiI CCNNNNNNNGG 2 cut(s) 506, 562
AgsI TTSAA 2 cut(s) 236, 278
AjuI GAANNNNNNNTTGG 2 cut(s) 367, 399
AleI CACNNNNGTG 1 cut(s) 78
AluBI AGCT 1 cut(s) 94
AluI AGCT 1 cut(s) 94
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 3 cut(s) 259, 302, 538
AlwI GGATC 2 cut(s) 203, 216
Ama87I CYCGRG 2 cut(s) 190, 521
AoxI GGCC 1 cut(s) 337
ApeKI GCWGC 2 cut(s) 94, 462
ApoI RAATTY 2 cut(s) 317, 514
AspS9I GGNCC 2 cut(s) 217, 338
AsuHPI GGTGA 4 cut(s) 79, 118, 497, 553
AvaI CYCGRG 2 cut(s) 190, 521
AvaII GGWCC 1 cut(s) 217
BamHI GGATCC 1 cut(s) 208
BanI GGYRCC 1 cut(s) 186
BarI GAAGNNNNNNTAC 2 cut(s) 474, 506
BauI CACGAG 2 cut(s) 74, 404
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 81, 474
BccI CCATC 1 cut(s) 208
BceAI ACGGC 2 cut(s) 82, 450
BcoDI GTCTC 3 cut(s) 259, 302, 538
BisI GCNGC 2 cut(s) 95, 463
BlsI GCNGC 2 cut(s) 96, 464
BmcAI AGTACT 1 cut(s) 432
Bme18I GGWCC 1 cut(s) 217
BmeT110I CYCGRG 2 cut(s) 190, 521
BmgT120I GGNCC 2 cut(s) 217, 338
BmiI GGNNCC 3 cut(s) 188, 210, 340
BmsI GCATC 2 cut(s) 438, 484
BpuEI CTTGAG 2 cut(s) 486, 542
BsaJI CCNNGG 4 cut(s) 102, 191, 220, 370
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 256, 286
Bsc4I CCNNNNNNNGG 2 cut(s) 506, 562
Bse118I RCCGGY 1 cut(s) 183
BseDI CCNNGG 4 cut(s) 102, 191, 220, 370
BseLI CCNNNNNNNGG 2 cut(s) 506, 562
BseRI GAGGAG 1 cut(s) 522
BseXI GCAGC 2 cut(s) 81, 474
BseYI CCCAGC 1 cut(s) 115
Bsh1236I CGCG 1 cut(s) 372
BshFI GGCC 1 cut(s) 339
BshNI GGYRCC 1 cut(s) 186
BsiHKAI GWGCWC 1 cut(s) 405
BsiHKCI CYCGRG 2 cut(s) 190, 521
BsiSI CCGG 1 cut(s) 184
BslI CCNNNNNNNGG 2 cut(s) 506, 562
BsmAI GTCTC 3 cut(s) 259, 302, 538
BsmBI CGTCTC 1 cut(s) 302
BsnI GGCC 1 cut(s) 339
BsoBI CYCGRG 2 cut(s) 190, 521
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 2 cut(s) 127, 208
Bsp19I CCATGG 2 cut(s) 102, 220
BspACI CCGC 4 cut(s) 16, 370, 372, 425
BspANI GGCC 1 cut(s) 339
BspFNI CGCG 1 cut(s) 372
BspLI GGNNCC 3 cut(s) 188, 210, 340
BspPI GGATC 2 cut(s) 203, 216
BspT107I GGYRCC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 183
BssAI RCCGGY 1 cut(s) 183
BssECI CCNNGG 4 cut(s) 102, 191, 220, 370
BssMI GATC 2 cut(s) 127, 208
BssSI CACGAG 2 cut(s) 74, 404
BssT1I CCWWGG 2 cut(s) 102, 220
Bst2BI CACGAG 2 cut(s) 74, 404
BstC8I GCNNGC 2 cut(s) 185, 473
BstDSI CCRYGG 3 cut(s) 102, 220, 370
BstENI CCTNNNNNAGG 1 cut(s) 504
BstFNI CGCG 1 cut(s) 372
BstKTI GATC 2 cut(s) 130, 211
BstMAI GTCTC 3 cut(s) 259, 302, 538
BstMBI GATC 2 cut(s) 127, 208
BstMWI GCNNNNNNNGC 3 cut(s) 193, 378, 468
BstNSI RCATGY 1 cut(s) 425
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 81, 474
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BsuRI GGCC 1 cut(s) 339
BtgI CCRYGG 3 cut(s) 102, 220, 370
Cac8I GCNNGC 2 cut(s) 185, 473
Cfr10I RCCGGY 1 cut(s) 183
Cfr13I GGNCC 2 cut(s) 217, 338
Cfr42I CCGCGG 1 cut(s) 373
Csp6I GTAC 2 cut(s) 139, 431
CviAII CATG 5 cut(s) 7, 103, 221, 330, 422
CviJI RGCY 5 cut(s) 11, 29, 94, 339, 465
CviKI_1 RGCY 5 cut(s) 11, 29, 94, 339, 465
CviQI GTAC 2 cut(s) 139, 431
DpnI GATC 2 cut(s) 129, 210
DpnII GATC 2 cut(s) 127, 208
Eco130I CCWWGG 2 cut(s) 102, 220
Eco47I GGWCC 1 cut(s) 217
Eco88I CYCGRG 2 cut(s) 190, 521
EcoNI CCTNNNNNAGG 1 cut(s) 504
EcoO109I RGGNCCY 1 cut(s) 338
EcoRI GAATTC 1 cut(s) 514
EcoT14I CCWWGG 2 cut(s) 102, 220
ErhI CCWWGG 2 cut(s) 102, 220
Esp3I CGTCTC 1 cut(s) 302
FaeI CATG 5 cut(s) 10, 106, 224, 333, 425
FatI CATG 5 cut(s) 6, 102, 220, 329, 421
Fnu4HI GCNGC 2 cut(s) 95, 463
Fsp4HI GCNGC 2 cut(s) 95, 463
GluI GCNGC 2 cut(s) 95, 463
GsaI CCCAGC 1 cut(s) 119
HaeIII GGCC 1 cut(s) 339
HapII CCGG 1 cut(s) 184
Hin1II CATG 5 cut(s) 10, 106, 224, 333, 425
HpaII CCGG 1 cut(s) 184
HphI GGTGA 4 cut(s) 79, 118, 497, 553
Hpy166II GTNNAC 1 cut(s) 36
Hpy188I TCNGA 2 cut(s) 48, 213
Hpy188III TCNNGA 3 cut(s) 275, 406, 479
Hpy8I GTNNAC 1 cut(s) 36
Hpy99I CGWCG 1 cut(s) 471
HpyAV CCTTC 4 cut(s) 242, 255, 329, 476
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 378, 468
Hsp92II CATG 5 cut(s) 10, 106, 224, 333, 425
KroI GCCGGC 1 cut(s) 183
KroNI GCCGGC 1 cut(s) 185
KspI CCGCGG 1 cut(s) 373
Kzo9I GATC 2 cut(s) 127, 208
LpnPI CCDG 2 cut(s) 129, 197
Lsp1109I GCAGC 2 cut(s) 81, 474
LweI GCATC 2 cut(s) 438, 484
MaeIII GTNAC 1 cut(s) 106
MalI GATC 2 cut(s) 129, 210
MboI GATC 2 cut(s) 127, 208
MboII GAAGA 1 cut(s) 400
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 405
MluCI AATT 6 cut(s) 39, 178, 200, 317, 415, 514
MmeI TCCRAC 2 cut(s) 71, 350
MroNI GCCGGC 1 cut(s) 183
MseI TTAA 2 cut(s) 321, 353
MslI CAYNNNNRTG 2 cut(s) 78, 398
MspA1I CMGCKG 1 cut(s) 372
MspI CCGG 1 cut(s) 184
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 3 cut(s) 193, 378, 468
NaeI GCCGGC 1 cut(s) 185
NcoI CCATGG 2 cut(s) 102, 220
NdeII GATC 2 cut(s) 127, 208
NgoMIV GCCGGC 1 cut(s) 183
NlaIII CATG 5 cut(s) 10, 106, 224, 333, 425
NlaIV GGNNCC 3 cut(s) 188, 210, 340
NmuCI GTSAC 1 cut(s) 106
NspI RCATGY 1 cut(s) 425
OliI CACNNNNGTG 1 cut(s) 78
PaeR7I CTCGAG 1 cut(s) 521
PdiI GCCGGC 1 cut(s) 185
PflFI GACNNNGTC 1 cut(s) 270
PkrI GCNGC 2 cut(s) 96, 464
PspFI CCCAGC 1 cut(s) 115
PspN4I GGNNCC 3 cut(s) 188, 210, 340
PspPI GGNCC 2 cut(s) 217, 338
PspXI VCTCGAGB 1 cut(s) 521
PsrI GAACNNNNNNTAC 2 cut(s) 131, 163
PsuI RGATCY 1 cut(s) 208
PsyI GACNNNGTC 1 cut(s) 270
RsaI GTAC 2 cut(s) 140, 432
RsaNI GTAC 2 cut(s) 139, 431
RseI CAYNNNNRTG 2 cut(s) 78, 398
SacII CCGCGG 1 cut(s) 373
SaqAI TTAA 2 cut(s) 321, 353
SatI GCNGC 2 cut(s) 95, 463
Sau3AI GATC 2 cut(s) 127, 208
Sau96I GGNCC 2 cut(s) 217, 338
ScaI AGTACT 1 cut(s) 432
SduI GDGCHC 1 cut(s) 405
SetI ASST 6 cut(s) 96, 487, 528, 532, 543, 564
SfaNI GCATC 2 cut(s) 438, 484
Sfr274I CTCGAG 1 cut(s) 521
Sfr303I CCGCGG 1 cut(s) 373
SgrBI CCGCGG 1 cut(s) 373
SinI GGWCC 1 cut(s) 217
SlaI CTCGAG 1 cut(s) 521
SmiMI CAYNNNNRTG 2 cut(s) 78, 398
SmlI CTYRAG 3 cut(s) 501, 521, 557
SmoI CTYRAG 3 cut(s) 501, 521, 557
Sse9I AATT 6 cut(s) 39, 178, 200, 317, 415, 514
SsiI CCGC 4 cut(s) 16, 370, 372, 425
StyI CCWWGG 2 cut(s) 102, 220
TaqI TCGA 3 cut(s) 204, 522, 537
TasI AATT 6 cut(s) 39, 178, 200, 317, 415, 514
TatI WGTACW 1 cut(s) 430
Tru1I TTAA 2 cut(s) 321, 353
Tru9I TTAA 2 cut(s) 321, 353
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 2 cut(s) 94, 462
Tsp45I GTSAC 1 cut(s) 106
TspGWI ACGGA 2 cut(s) 318, 326
Tth111I GACNNNGTC 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 217
XagI CCTNNNNNAGG 1 cut(s) 504
XapI RAATTY 2 cut(s) 317, 514
XceI RCATGY 1 cut(s) 425
XhoI CTCGAG 1 cut(s) 521
ZrmI AGTACT 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.