Rroxscaffold_3G00256390

Histone deacetylase complex subunit SAP18-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005137
Physical Location & Seq
Reverse (-)
50754910 .. 50756891
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256390.1

Sequence Viewer

Length: 516 bp
ATGGAGAGGAGAAGCAGAGACGAAAAGATGGGCGGAGGAGGAGGAGGACCTGGTCCCCGAGGTGGCCCACCCGGTCGACCTCTCCCTCCACCTCCTCCTCTTCCTCAGTCCCGCGGCCCACCTCCCCCGCCGCCCCGCCGCTTCGAACCCGTCGACCGTGAAAAGACTTGCCCTTTGTTGCTTCGGGTCTTCACTAAGATTGGAGGTCATCACAATCAGGAGGACTTTGCAGTGAGAGGCAAAGAACCCAAAGATGAGGTTCAGATTTATACATGGAAAGATGCCACACTACGCGAACTAACTGATCTTGTCAAGGAGATTGCCCCAGCGGCTAGGAGAAGAAATGCAAAACTGTCATTTGCTTTTGTATACCCTGACAAGGGTGGTCGTTTTGTCTTGAAACAGGCGGGGATAACACATGCTTATGGAAACATGGGGAGACGTCTAGATGACAGCAAGTCCTTGAATGAGCTCAAGTTCCAGATTGGAGATTATTTGGATGTGGCAGTCCTATAG

Protein Analysis

171

Amino Acids

18.98

Weight (kDa)

9.89

Isoelectric Point (pI)

63.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP18 PF06487 51 - 170 1.4e-40 Sin3 associated polypeptide p18 (SAP18)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 445
AccI GTMKAC 3 cut(s) 76, 153, 369
AccII CGCG 2 cut(s) 114, 294
AciI CCGC 9 cut(s) 33, 112, 114, 128, 131, 136, 139, 329, 407
AcyI GRCGYC 1 cut(s) 442
AfiI CCNNNNNNNGG 3 cut(s) 62, 379, 380
AgsI TTSAA 2 cut(s) 400, 466
AhdI GACNNNNNGTC 1 cut(s) 457
AjnI CCWGG 1 cut(s) 49
AluBI AGCT 1 cut(s) 472
AluI AGCT 1 cut(s) 472
Alw21I GWGCWC 1 cut(s) 474
Alw26I GTCTC 2 cut(s) 12, 433
Ama87I CYCGRG 1 cut(s) 57
AoxI GGCC 2 cut(s) 64, 115
ArsI GACNNNNNNTTYG 2 cut(s) 157, 189
AspS9I GGNCC 4 cut(s) 47, 53, 65, 116
AsuC2I CCSGG 1 cut(s) 72
AsuII TTCGAA 1 cut(s) 144
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 2 cut(s) 47, 53
BanII GRGCYC 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 474
BccI CCATC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 51
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 2 cut(s) 12, 433
BfaI CTAG 2 cut(s) 333, 446
BfmI CTRYAG 1 cut(s) 512
BglI GCCNNNNNGGC 1 cut(s) 329
BisI GCNGC 4 cut(s) 115, 131, 139, 330
BlsI GCNGC 4 cut(s) 116, 132, 140, 331
Bme1390I CCNGG 2 cut(s) 51, 72
Bme18I GGWCC 2 cut(s) 47, 53
BmeRI GACNNNNNGTC 1 cut(s) 457
BmeT110I CYCGRG 1 cut(s) 57
BmgT120I GGNCC 4 cut(s) 47, 53, 65, 116
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 51, 72
BmsI GCATC 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 181
Bpu14I TTCGAA 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 442
BsaJI CCNNGG 2 cut(s) 58, 112
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 379, 380
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 58, 112
BseGI GGATG 1 cut(s) 505
BseLI CCNNNNNNNGG 3 cut(s) 62, 379, 380
BseMII CTCAG 1 cut(s) 119
BseRI GAGGAG 6 cut(s) 22, 51, 54, 57, 84, 87
BseYI CCCAGC 1 cut(s) 325
Bsh1236I CGCG 2 cut(s) 114, 294
Bsh1285I CGRYCG 2 cut(s) 76, 157
BshFI GGCC 2 cut(s) 66, 117
BsiEI CGRYCG 2 cut(s) 76, 157
BsiHKAI GWGCWC 1 cut(s) 474
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 2 cut(s) 39, 94
BslI CCNNNNNNNGG 3 cut(s) 62, 379, 380
BsmAI GTCTC 2 cut(s) 12, 433
BsmBI CGTCTC 2 cut(s) 12, 433
BsmFI GGGAC 2 cut(s) 39, 94
BsnI GGCC 2 cut(s) 66, 117
BsoBI CYCGRG 1 cut(s) 57
Bsp119I TTCGAA 1 cut(s) 144
Bsp1286I GDGCHC 1 cut(s) 474
Bsp143I GATC 1 cut(s) 304
BspACI CCGC 9 cut(s) 33, 112, 114, 128, 131, 136, 139, 329, 407
BspANI GGCC 2 cut(s) 66, 117
BspCNI CTCAG 1 cut(s) 118
BspFNI CGCG 2 cut(s) 114, 294
BspLI GGNNCC 1 cut(s) 55
BspT104I TTCGAA 1 cut(s) 144
BssECI CCNNGG 2 cut(s) 58, 112
BssMI GATC 1 cut(s) 304
BssNAI GTATAC 1 cut(s) 370
BssNI GRCGYC 1 cut(s) 442
Bst1107I GTATAC 1 cut(s) 370
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 2 cut(s) 158, 354
Bst6I CTCTTC 1 cut(s) 105
BstACI GRCGYC 1 cut(s) 442
BstBI TTCGAA 1 cut(s) 144
BstDEI CTNAG 2 cut(s) 105, 195
BstDSI CCRYGG 1 cut(s) 112
BstF5I GGATG 1 cut(s) 505
BstFNI CGCG 2 cut(s) 114, 294
BstKTI GATC 1 cut(s) 307
BstMAI GTCTC 2 cut(s) 12, 433
BstMBI GATC 1 cut(s) 304
BstMCI CGRYCG 2 cut(s) 76, 157
BstMWI GCNNNNNNNGC 1 cut(s) 329
BstNI CCWGG 1 cut(s) 51
BstNSI RCATGY 1 cut(s) 422
BstSCI CCNGG 2 cut(s) 49, 70
BstSFI CTRYAG 1 cut(s) 512
BstUI CGCG 2 cut(s) 114, 294
BstV2I GAAGAC 1 cut(s) 181
BstZ17I GTATAC 1 cut(s) 370
BsuRI GGCC 2 cut(s) 66, 117
BtgI CCRYGG 1 cut(s) 112
BtsCI GGATG 1 cut(s) 505
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 237
Cfr13I GGNCC 4 cut(s) 47, 53, 65, 116
Cfr42I CCGCGG 1 cut(s) 115
CsiI ACCWGGT 1 cut(s) 49
CviAII CATG 3 cut(s) 273, 419, 433
CviJI RGCY 4 cut(s) 66, 117, 332, 472
CviKI_1 RGCY 4 cut(s) 66, 117, 332, 472
DdeI CTNAG 2 cut(s) 105, 195
DpnI GATC 1 cut(s) 306
DpnII GATC 1 cut(s) 304
DriI GACNNNNNGTC 1 cut(s) 457
Eam1104I CTCTTC 1 cut(s) 105
Eam1105I GACNNNNNGTC 1 cut(s) 457
EarI CTCTTC 1 cut(s) 105
EciI GGCGGA 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 472
Eco24I GRGCYC 1 cut(s) 474
Eco47I GGWCC 2 cut(s) 47, 53
Eco53kI GAGCTC 1 cut(s) 472
Eco88I CYCGRG 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 472
EcoO109I RGGNCCY 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 49
EcoT38I GRGCYC 1 cut(s) 474
Esp3I CGTCTC 2 cut(s) 12, 433
FaeI CATG 3 cut(s) 276, 422, 436
FaiI YATR 7 cut(s) 270, 274, 370, 420, 426, 434, 514
FaqI GGGAC 2 cut(s) 39, 94
FatI CATG 3 cut(s) 272, 418, 432
FauI CCCGC 4 cut(s) 119, 135, 143, 400
FblI GTMKAC 3 cut(s) 76, 153, 369
Fnu4HI GCNGC 4 cut(s) 115, 131, 139, 330
FokI GGATG 1 cut(s) 512
FriOI GRGCYC 1 cut(s) 474
Fsp4HI GCNGC 4 cut(s) 115, 131, 139, 330
FspBI CTAG 2 cut(s) 333, 446
GluI GCNGC 4 cut(s) 115, 131, 139, 330
GsaI CCCAGC 1 cut(s) 329
HaeIII GGCC 2 cut(s) 66, 117
HapII CCGG 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 442
Hin1II CATG 3 cut(s) 276, 422, 436
HincII GTYRAC 2 cut(s) 77, 154
HindII GTYRAC 2 cut(s) 77, 154
HpaII CCGG 1 cut(s) 72
Hpy166II GTNNAC 3 cut(s) 77, 154, 370
Hpy188I TCNGA 1 cut(s) 264
Hpy188III TCNNGA 4 cut(s) 218, 397, 446, 481
Hpy8I GTNNAC 3 cut(s) 77, 154, 370
Hpy99I CGWCG 1 cut(s) 155
HpyCH4III ACNGT 2 cut(s) 158, 354
HpyCH4IV ACGT 1 cut(s) 442
HpyCH4V TGCA 2 cut(s) 230, 347
HpyF10VI GCNNNNNNNGC 1 cut(s) 329
HpyF3I CTNAG 2 cut(s) 105, 195
HpySE526I ACGT 1 cut(s) 442
Hsp92I GRCGYC 1 cut(s) 442
Hsp92II CATG 3 cut(s) 276, 422, 436
KspI CCGCGG 1 cut(s) 115
Kzo9I GATC 1 cut(s) 304
LpnPI CCDG 8 cut(s) 36, 63, 85, 203, 339, 387, 389, 494
LweI GCATC 1 cut(s) 271
MabI ACCWGGT 1 cut(s) 49
MaeI CTAG 2 cut(s) 333, 446
MaeII ACGT 1 cut(s) 442
MalI GATC 1 cut(s) 306
MboI GATC 1 cut(s) 304
MboII GAAGA 3 cut(s) 92, 181, 351
MhlI GDGCHC 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 423
MspA1I CMGCKG 2 cut(s) 114, 329
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 2 cut(s) 51, 72
MvaI CCWGG 1 cut(s) 51
MvnI CGCG 2 cut(s) 114, 294
MwoI GCNNNNNNNGC 1 cut(s) 329
NciI CCSGG 1 cut(s) 72
NdeII GATC 1 cut(s) 304
NlaIII CATG 3 cut(s) 276, 422, 436
NlaIV GGNNCC 1 cut(s) 55
NspI RCATGY 1 cut(s) 422
NspV TTCGAA 1 cut(s) 144
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PflFI GACNNNGTC 1 cut(s) 51
PkrI GCNGC 4 cut(s) 116, 132, 140, 331
PpuMI RGGWCCY 1 cut(s) 47
Psp124BI GAGCTC 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 49
PspFI CCCAGC 1 cut(s) 325
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 4 cut(s) 47, 53, 65, 116
PspPPI RGGWCCY 1 cut(s) 47
PsyI GACNNNGTC 1 cut(s) 51
RseI CAYNNNNRTG 1 cut(s) 423
SacI GAGCTC 1 cut(s) 474
SacII CCGCGG 1 cut(s) 115
SalI GTCGAC 2 cut(s) 75, 152
SatI GCNGC 4 cut(s) 115, 131, 139, 330
Sau3AI GATC 1 cut(s) 304
Sau96I GGNCC 4 cut(s) 47, 53, 65, 116
ScrFI CCNGG 2 cut(s) 51, 72
SduI GDGCHC 1 cut(s) 474
SetI ASST 9 cut(s) 52, 64, 82, 94, 124, 208, 261, 445, 474
SexAI ACCWGGT 1 cut(s) 49
SfaNI GCATC 1 cut(s) 271
SfcI CTRYAG 1 cut(s) 512
Sfr303I CCGCGG 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 144
SgrBI CCGCGG 1 cut(s) 115
SinI GGWCC 2 cut(s) 47, 53
SmiMI CAYNNNNRTG 1 cut(s) 423
SmlI CTYRAG 1 cut(s) 473
SmoI CTYRAG 1 cut(s) 473
SsiI CCGC 9 cut(s) 33, 112, 114, 128, 131, 136, 139, 329, 407
SspMI CTAG 2 cut(s) 333, 446
SstI GAGCTC 1 cut(s) 474
StyD4I CCNGG 2 cut(s) 49, 70
TaaI ACNGT 2 cut(s) 158, 354
TaiI ACGT 1 cut(s) 445
TaqI TCGA 3 cut(s) 76, 144, 153
TauI GCSGC 4 cut(s) 117, 133, 141, 332
TscAI CASTG 1 cut(s) 237
TspRI CASTG 1 cut(s) 237
Tth111I GACNNNGTC 1 cut(s) 51
VpaK11BI GGWCC 2 cut(s) 47, 53
XbaI TCTAGA 1 cut(s) 445
XceI RCATGY 1 cut(s) 422
XmiI GTMKAC 3 cut(s) 76, 153, 369
XspI CTAG 2 cut(s) 333, 446
ZraI GACGTC 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.