Rroxscaffold_4G00279850

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003948
Physical Location & Seq
Forward (+)
2520061 .. 2520774
714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00279850.1

Sequence Viewer

Length: 714 bp
ATGCGGGAGGTCGACGGTAAGCTATACGCTCTCTCTGGCGATTACATGGAAGTTGCGGCTTTTGAGGTATTTGACCCCAAAACCAATCATGTGTGGGCTCCTCTCCCCAGTCTTCCAATTGGGAATGATAGGCCTCCCTGGAAAGCGCTTTCTTCATCCTCCTGTGCAGTTGTAGGCACCAAGATCTTGGTCTCACTTACGTTAGACTCTAGGGAGACAGTAGTGAAGATACTCTGCTTCGACGTGGCTCACCCTGAGAGATCATGGACTAGACTTGAACCTCCCTGGTGGCAGTTTCATAGTTCTTTCCCCGAATTTTACAACGAGGTTTTAGTGTTGGACTTGGAGGATGACGACTGTGAGAAGCTATTGTTTACTTATGATTTTAATTATTGCCAAATACGAGTCTCCAGCATGAAGCTGTCCAATGAAGGTGAACCTAAGTGTATTCAACCGCTAGATGACATAGATTTGCCTGATGAGTTTAAGAACTTGGATGATTTTTTAGCAACTTCATACAGCTGTGCCCATCTTGGAGGTCGAAGGGTATGCTTTTCATTTGTGCATTTGCTAACTCTTCTCGTGTTCTCTTTCGAATTTTCCTATCCGATTTCTGGATCATTCTCTGTCAAAGCCCTGGGTCATCGCCTGTTCGAATACAACTCGAGTGAGGAACTTTCTTCTGTCAAATTGGGTGGTTGCTTTGTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

237

Amino Acids

26.94

Weight (kDa)

4.76

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 176
AccI GTMKAC 1 cut(s) 12
AciI CCGC 3 cut(s) 4, 56, 455
AclWI GGATC 1 cut(s) 625
AcsI RAATTY 2 cut(s) 314, 596
AfeI AGCGCT 1 cut(s) 147
AfiI CCNNNNNNNGG 1 cut(s) 614
AgsI TTSAA 2 cut(s) 278, 452
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 3 cut(s) 137, 284, 636
AluBI AGCT 4 cut(s) 22, 367, 421, 522
AluI AGCT 4 cut(s) 22, 367, 421, 522
Alw26I GTCTC 3 cut(s) 196, 209, 412
AlwI GGATC 1 cut(s) 625
Ama87I CYCGRG 1 cut(s) 664
Aor51HI AGCGCT 1 cut(s) 147
AoxI GGCC 1 cut(s) 131
ApoI RAATTY 2 cut(s) 314, 596
AspLEI GCGC 1 cut(s) 148
AsuHPI GGTGA 2 cut(s) 242, 446
AsuII TTCGAA 2 cut(s) 594, 654
AvaI CYCGRG 1 cut(s) 664
BaeGI GKGCMC 1 cut(s) 529
BanI GGYRCC 1 cut(s) 176
BanII GRGCYC 1 cut(s) 100
BauI CACGAG 1 cut(s) 581
BbsI GAAGAC 1 cut(s) 104
BccI CCATC 1 cut(s) 537
BcgI CGANNNNNNTGC 2 cut(s) 531, 565
BciT130I CCWGG 3 cut(s) 139, 286, 638
BcoDI GTCTC 3 cut(s) 196, 209, 412
BfaI CTAG 3 cut(s) 210, 270, 458
BfoI RGCGCY 1 cut(s) 149
BglII AGATCT 1 cut(s) 183
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
Bme1390I CCNGG 3 cut(s) 139, 286, 638
BmeT110I CYCGRG 1 cut(s) 664
BmgBI CACGTC 1 cut(s) 244
BmiI GGNNCC 2 cut(s) 99, 178
BmrFI CCNGG 3 cut(s) 139, 286, 638
BmrI ACTGGG 1 cut(s) 102
BmuI ACTGGG 1 cut(s) 102
BpiI GAAGAC 1 cut(s) 104
BpmI CTGGAG 1 cut(s) 394
Bpu14I TTCGAA 2 cut(s) 594, 654
BsaI GGTCTC 1 cut(s) 196
BsaJI CCNNGG 4 cut(s) 137, 284, 636, 637
Bsc4I CCNNNNNNNGG 1 cut(s) 614
Bse1I ACTGG 1 cut(s) 108
BseBI CCWGG 3 cut(s) 139, 286, 638
BseDI CCNNGG 4 cut(s) 137, 284, 636, 637
BseGI GGATG 3 cut(s) 155, 355, 502
BseLI CCNNNNNNNGG 1 cut(s) 614
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 108
BseRI GAGGAG 1 cut(s) 90
BseSI GKGCMC 1 cut(s) 529
BsgI GTGCAG 1 cut(s) 186
BshFI GGCC 1 cut(s) 133
BshNI GGYRCC 1 cut(s) 176
BsiHKCI CYCGRG 1 cut(s) 664
BslI CCNNNNNNNGG 1 cut(s) 614
BsmAI GTCTC 3 cut(s) 196, 209, 412
BsnI GGCC 1 cut(s) 133
Bso31I GGTCTC 1 cut(s) 196
BsoBI CYCGRG 1 cut(s) 664
Bsp119I TTCGAA 2 cut(s) 594, 654
Bsp1286I GDGCHC 2 cut(s) 100, 529
Bsp143I GATC 3 cut(s) 183, 260, 617
BspACI CCGC 3 cut(s) 4, 56, 455
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 99, 178
BspPI GGATC 1 cut(s) 625
BspT104I TTCGAA 2 cut(s) 594, 654
BspT107I GGYRCC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 196
BsrI ACTGG 1 cut(s) 108
BssECI CCNNGG 4 cut(s) 137, 284, 636, 637
BssMI GATC 3 cut(s) 183, 260, 617
BssSI CACGAG 1 cut(s) 581
Bst2BI CACGAG 1 cut(s) 581
Bst2UI CCWGG 3 cut(s) 139, 286, 638
Bst4CI ACNGT 3 cut(s) 17, 220, 359
Bst6I CTCTTC 1 cut(s) 582
BstBI TTCGAA 2 cut(s) 594, 654
BstDEI CTNAG 2 cut(s) 255, 441
BstF5I GGATG 3 cut(s) 155, 355, 502
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 3 cut(s) 186, 263, 620
BstMAI GTCTC 3 cut(s) 196, 209, 412
BstMBI GATC 3 cut(s) 183, 260, 617
BstNI CCWGG 3 cut(s) 139, 286, 638
BstSCI CCNGG 3 cut(s) 137, 284, 636
BstSLI GKGCMC 1 cut(s) 529
BstV2I GAAGAC 1 cut(s) 104
BstX2I RGATCY 1 cut(s) 183
BstXI CCANNNNNNTGG 1 cut(s) 187
BstYI RGATCY 1 cut(s) 183
BsuRI GGCC 1 cut(s) 133
BtgZI GCGATG 1 cut(s) 629
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 3 cut(s) 155, 355, 502
CfoI GCGC 1 cut(s) 148
CspCI CAANNNNNGTGG 2 cut(s) 676, 711
CviAII CATG 4 cut(s) 46, 89, 264, 415
CviJI RGCY 9 cut(s) 22, 59, 98, 133, 248, 367, 421, 522, 635
CviKI_1 RGCY 9 cut(s) 22, 59, 98, 133, 248, 367, 421, 522, 635
DdeI CTNAG 2 cut(s) 255, 441
DpnI GATC 3 cut(s) 185, 262, 619
DpnII GATC 3 cut(s) 183, 260, 617
Eam1104I CTCTTC 1 cut(s) 582
EarI CTCTTC 1 cut(s) 582
Eco147I AGGCCT 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 100
Eco31I GGTCTC 1 cut(s) 196
Eco47III AGCGCT 1 cut(s) 147
Eco88I CYCGRG 1 cut(s) 664
EcoRII CCWGG 3 cut(s) 137, 284, 636
EcoT38I GRGCYC 1 cut(s) 100
FaeI CATG 4 cut(s) 49, 92, 267, 418
FatI CATG 4 cut(s) 45, 88, 263, 414
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 1 cut(s) 57
FokI GGATG 3 cut(s) 142, 362, 509
FriOI GRGCYC 1 cut(s) 100
Fsp4HI GCNGC 1 cut(s) 57
FspBI CTAG 3 cut(s) 210, 270, 458
GlaI GCGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 57
GsuI CTGGAG 1 cut(s) 394
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 1 cut(s) 133
HhaI GCGC 1 cut(s) 148
Hin1II CATG 4 cut(s) 49, 92, 267, 418
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 2 cut(s) 206, 405
HphI GGTGA 2 cut(s) 242, 446
Hpy166II GTNNAC 3 cut(s) 13, 375, 437
Hpy188I TCNGA 1 cut(s) 609
Hpy188III TCNNGA 1 cut(s) 615
Hpy8I GTNNAC 3 cut(s) 13, 375, 437
Hpy99I CGWCG 2 cut(s) 17, 245
HpyAV CCTTC 2 cut(s) 425, 537
HpyCH4III ACNGT 3 cut(s) 17, 220, 359
HpyCH4IV ACGT 2 cut(s) 200, 243
HpyCH4V TGCA 2 cut(s) 167, 565
HpyF3I CTNAG 2 cut(s) 255, 441
HpySE526I ACGT 2 cut(s) 200, 243
Hsp92II CATG 4 cut(s) 49, 92, 267, 418
HspAI GCGC 1 cut(s) 146
Kzo9I GATC 3 cut(s) 183, 260, 617
LmnI GCTCC 1 cut(s) 103
MaeI CTAG 3 cut(s) 210, 270, 458
MaeII ACGT 2 cut(s) 200, 243
MalI GATC 3 cut(s) 185, 262, 619
MboI GATC 3 cut(s) 183, 260, 617
MboII GAAGA 5 cut(s) 104, 144, 238, 569, 672
MfeI CAATTG 1 cut(s) 117
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 2 cut(s) 100, 529
MluCI AATT 5 cut(s) 117, 314, 388, 596, 689
MlyI GAGTC 2 cut(s) 200, 414
MmeI TCCRAC 1 cut(s) 318
MnlI CCTC 9 cut(s) 58, 111, 144, 169, 291, 319, 340, 530, 664
MseI TTAA 2 cut(s) 387, 486
MspA1I CMGCKG 1 cut(s) 522
MspR9I CCNGG 3 cut(s) 139, 286, 638
MunI CAATTG 1 cut(s) 117
MvaI CCWGG 3 cut(s) 139, 286, 638
NdeII GATC 3 cut(s) 183, 260, 617
NlaIII CATG 4 cut(s) 49, 92, 267, 418
NlaIV GGNNCC 2 cut(s) 99, 178
NspV TTCGAA 2 cut(s) 594, 654
PaeR7I CTCGAG 1 cut(s) 664
PasI CCCWGGG 1 cut(s) 637
PceI AGGCCT 1 cut(s) 133
PkrI GCNGC 1 cut(s) 58
PleI GAGTC 2 cut(s) 200, 413
PpsI GAGTC 2 cut(s) 200, 413
Psp6I CCWGG 3 cut(s) 137, 284, 636
PspGI CCWGG 3 cut(s) 137, 284, 636
PspN4I GGNNCC 2 cut(s) 99, 178
PspXI VCTCGAGB 1 cut(s) 664
PsuI RGATCY 1 cut(s) 183
PvuII CAGCTG 1 cut(s) 522
SalI GTCGAC 1 cut(s) 11
SaqAI TTAA 2 cut(s) 387, 486
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 3 cut(s) 183, 260, 617
SchI GAGTC 2 cut(s) 200, 414
ScrFI CCNGG 3 cut(s) 139, 286, 638
SduI GDGCHC 2 cut(s) 100, 529
Sfr274I CTCGAG 1 cut(s) 664
SfuI TTCGAA 2 cut(s) 594, 654
SlaI CTCGAG 1 cut(s) 664
SmlI CTYRAG 1 cut(s) 664
SmoI CTYRAG 1 cut(s) 664
Sse9I AATT 5 cut(s) 117, 314, 388, 596, 689
SseBI AGGCCT 1 cut(s) 133
SsiI CCGC 3 cut(s) 4, 56, 455
SspMI CTAG 3 cut(s) 210, 270, 458
StuI AGGCCT 1 cut(s) 133
StyD4I CCNGG 3 cut(s) 137, 284, 636
TaaI ACNGT 3 cut(s) 17, 220, 359
TaiI ACGT 2 cut(s) 203, 246
TaqI TCGA 6 cut(s) 12, 240, 541, 594, 654, 665
TasI AATT 5 cut(s) 117, 314, 388, 596, 689
TauI GCSGC 1 cut(s) 59
Tru1I TTAA 2 cut(s) 387, 486
Tru9I TTAA 2 cut(s) 387, 486
TspDTI ATGAA 6 cut(s) 144, 287, 431, 444, 504, 546
XapI RAATTY 2 cut(s) 314, 596
XhoI CTCGAG 1 cut(s) 664
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 3 cut(s) 210, 270, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.