Rh1DG422200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0003948
Physical Location & Seq
Reverse (-)
63648989 .. 63650224
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG422200.1

Sequence Viewer

Length: 1236 bp
ATGGGTGGAGCAGCAAGGTTTCGGGCGAGGGCGAAGAAGAGGGCGGAAAAAGAAGAGGAGAGTAGGAAAGCCTACGAGGCCCGGGAAAGGAAGAAACCCGACGAGGCTGAATCATTGCTGTATCTATGCACTTCCGAGATCTACGACCATAAAGGTTTAGCTGGATACCGTGTCCAGGTCATCAGATTGAGCGACTTGTTCATTCCTTCCGATACTCCCATCACCATATTTGGCCCCAAATCCAACTCCAACGAGGTGCTACAACAAGTTGCTTATTTAGACGGCGAACACGTTCCCGGCCATATGGGTTTTGGTGTCTTGGGCTCTCGAATTGTTCTGGCCGGCGGCTGGTTTCTCAATCCGTGGATGAGACAGAGACCTAAACCAACCTATGTTTTGGAGACTGATCGAAGTGTGGAACCCAACCCCAGGTTCATCAGCACCAGAGGTACTCCTAATCCTAAACCCCCCTATATGATTCCGGAATTGGAACCGGAAAAACCTCGTCCGTGGATGCGGGAGGTCGACGGTAAGCTATACGCTCTCTCTGGCGATTACATGGAAGTTGCGGCTTTTGAGGTATTTGACCCCAAAACCAATCATGTGTGGGCTCCTCTCCCCAGTCTTCCAATTGGGAATGATAGACCTCCCTGGAAAGCGCTTTCTTCATCCTCCTGTGCAGTTGTAGGCACCAAGATCTTGGTCACACTTACGTTAGACTCTAGGGCCTCTACGGCGACAGTAGTGAAGATACTCTGCTTCGACGTGGCTCACCCTGAGAGATCATGGACTAGACTTGAACCACCCTGGTGGCGGTTTCATAGTTCTTTCCCCGAATTTTACAACGAGGTTTTAGTGTTGGACTTGGAGGATGACGACTGTGAGAAGCTATTGTTTACTTATGATTTTGATTATTGCCAAATACGAGTCTCCAGCATGAAGCTGTCCAATGATGGTGAACCTAATTGTATTCAACCGCTAGATGAGATAGATTTGCCTGATGAGTTTAAGAACATGGATGATTTTTTAGCAACTTCATACAGCTGTGCTCATCTTGGAGGTCGAAGAGTATGCTTTTCATTCGTGCATTTGCTAACTCTTCTTGTGTTCACTTTCGAATTTTCCTATTCGATTTCTGGATCATTCTTTGTCAAAGCCCTGGGTCATCGCCTGTTCGAGTACAACTCAAGTGAGGAACTTTCTTCTGTCAAGTTGGGTGGTTGCTTTGTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

411

Amino Acids

46.74

Weight (kDa)

5.61

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 159 - 266 8.1e-07 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 689
AccI GTMKAC 1 cut(s) 525
AccIII TCCGGA 1 cut(s) 481
AciI CCGC 6 cut(s) 44, 345, 517, 569, 814, 977
AclWI GGATC 1 cut(s) 1147
AcoI YGGCCR 2 cut(s) 298, 339
AcsI RAATTY 2 cut(s) 836, 1118
AfaI GTAC 2 cut(s) 451, 1181
AfeI AGCGCT 1 cut(s) 660
AfiI CCNNNNNNNGG 5 cut(s) 87, 175, 348, 429, 813
AflIII ACRYGT 1 cut(s) 289
AgsI TTSAA 2 cut(s) 800, 974
AjiI CACGTC 1 cut(s) 766
AjnI CCWGG 5 cut(s) 174, 428, 650, 806, 1158
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AleI CACNNNNGTG 1 cut(s) 808
AluBI AGCT 5 cut(s) 161, 535, 889, 943, 1044
AluI AGCT 5 cut(s) 161, 535, 889, 943, 1044
Alw21I GWGCWC 1 cut(s) 1051
Alw26I GTCTC 4 cut(s) 364, 370, 395, 934
AlwI GGATC 1 cut(s) 1147
Ama87I CYCGRG 1 cut(s) 81
Aor13HI TCCGGA 1 cut(s) 481
Aor51HI AGCGCT 1 cut(s) 660
AoxI GGCC 5 cut(s) 78, 232, 298, 339, 726
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 2 cut(s) 836, 1118
Asp700I GAANNNNTTC 1 cut(s) 291
AspLEI GCGC 1 cut(s) 661
AspS9I GGNCC 3 cut(s) 79, 233, 726
AsuC2I CCSGG 3 cut(s) 82, 83, 297
AsuHPI GGTGA 3 cut(s) 214, 764, 968
AsuII TTCGAA 1 cut(s) 1116
AvaI CYCGRG 1 cut(s) 81
BanI GGYRCC 1 cut(s) 689
BanII GRGCYC 2 cut(s) 326, 613
BbsI GAAGAC 1 cut(s) 617
Bbv12I GWGCWC 1 cut(s) 1051
BbvI GCAGC 1 cut(s) 23
BccI CCATC 2 cut(s) 227, 947
BceAI ACGGC 2 cut(s) 298, 750
BcgI CGANNNNNNTGC 2 cut(s) 1053, 1087
BciT130I CCWGG 5 cut(s) 176, 430, 652, 808, 1160
BciVI GTATCC 1 cut(s) 158
BcnI CCSGG 3 cut(s) 82, 83, 297
BcoDI GTCTC 4 cut(s) 364, 370, 395, 934
BfaI CTAG 3 cut(s) 723, 792, 980
BfoI RGCGCY 1 cut(s) 662
BfuI GTATCC 1 cut(s) 158
BglI GCCNNNNNGGC 2 cut(s) 77, 734
BglII AGATCT 2 cut(s) 138, 696
BisI GCNGC 3 cut(s) 12, 346, 570
BlsI GCNGC 3 cut(s) 13, 347, 571
Bme1390I CCNGG 8 cut(s) 82, 83, 176, 297, 430, 652, 808, 1160
BmeT110I CYCGRG 1 cut(s) 81
BmgBI CACGTC 1 cut(s) 766
BmgT120I GGNCC 3 cut(s) 79, 233, 726
BmiI GGNNCC 5 cut(s) 235, 420, 492, 612, 691
BmrFI CCNGG 8 cut(s) 82, 83, 176, 297, 430, 652, 808, 1160
BmrI ACTGGG 1 cut(s) 615
BmsI GCATC 1 cut(s) 504
BmuI ACTGGG 1 cut(s) 615
BpiI GAAGAC 1 cut(s) 617
BplI GAGNNNNNCTC 2 cut(s) 1169, 1201
BpmI CTGGAG 1 cut(s) 916
Bpu14I TTCGAA 1 cut(s) 1116
BpuEI CTTGAG 1 cut(s) 1171
BpuMI CCSGG 3 cut(s) 82, 83, 297
BsaI GGTCTC 1 cut(s) 370
BsaJI CCNNGG 8 cut(s) 81, 362, 428, 509, 650, 806, 1158, 1159
BsaWI WCCGGW 2 cut(s) 481, 493
Bsc4I CCNNNNNNNGG 5 cut(s) 87, 175, 348, 429, 813
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 1 cut(s) 621
Bse3DI GCAATG 1 cut(s) 113
BseAI TCCGGA 1 cut(s) 481
BseBI CCWGG 5 cut(s) 176, 430, 652, 808, 1160
BseDI CCNNGG 8 cut(s) 81, 362, 428, 509, 650, 806, 1158, 1159
BseGI GGATG 5 cut(s) 372, 519, 668, 877, 1024
BseLI CCNNNNNNNGG 5 cut(s) 87, 175, 348, 429, 813
BseMI GCAATG 1 cut(s) 113
BseMII CTCAG 1 cut(s) 768
BseNI ACTGG 1 cut(s) 621
BseRI GAGGAG 2 cut(s) 71, 603
BseXI GCAGC 1 cut(s) 23
BsgI GTGCAG 1 cut(s) 699
BshFI GGCC 5 cut(s) 80, 234, 300, 341, 728
BshNI GGYRCC 1 cut(s) 689
BsiHKAI GWGCWC 1 cut(s) 1051
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 5 cut(s) 82, 297, 342, 482, 494
BslI CCNNNNNNNGG 5 cut(s) 87, 175, 348, 429, 813
BsmAI GTCTC 4 cut(s) 364, 370, 395, 934
BsnI GGCC 5 cut(s) 80, 234, 300, 341, 728
Bso31I GGTCTC 1 cut(s) 370
BsoBI CYCGRG 1 cut(s) 81
Bsp119I TTCGAA 1 cut(s) 1116
Bsp1286I GDGCHC 3 cut(s) 326, 613, 1051
Bsp13I TCCGGA 1 cut(s) 481
Bsp143I GATC 5 cut(s) 138, 406, 696, 782, 1139
BspACI CCGC 6 cut(s) 44, 345, 517, 569, 814, 977
BspANI GGCC 5 cut(s) 80, 234, 300, 341, 728
BspCNI CTCAG 1 cut(s) 769
BspEI TCCGGA 1 cut(s) 481
BspLI GGNNCC 5 cut(s) 235, 420, 492, 612, 691
BspPI GGATC 1 cut(s) 1147
BspT104I TTCGAA 1 cut(s) 1116
BspT107I GGYRCC 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 370
BsrDI GCAATG 1 cut(s) 113
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 1 cut(s) 621
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 8 cut(s) 81, 362, 428, 509, 650, 806, 1158, 1159
BssMI GATC 5 cut(s) 138, 406, 696, 782, 1139
Bst2UI CCWGG 5 cut(s) 176, 430, 652, 808, 1160
Bst4CI ACNGT 4 cut(s) 170, 530, 742, 881
Bst6I CTCTTC 4 cut(s) 32, 48, 1060, 1104
BstBI TTCGAA 1 cut(s) 1116
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 1 cut(s) 777
BstDSI CCRYGG 2 cut(s) 362, 509
BstF5I GGATG 5 cut(s) 372, 519, 668, 877, 1024
BstH2I RGCGCY 1 cut(s) 662
BstHHI GCGC 1 cut(s) 661
BstKTI GATC 5 cut(s) 141, 409, 699, 785, 1142
BstMAI GTCTC 4 cut(s) 364, 370, 395, 934
BstMBI GATC 5 cut(s) 138, 406, 696, 782, 1139
BstMWI GCNNNNNNNGC 2 cut(s) 77, 734
BstNI CCWGG 5 cut(s) 176, 430, 652, 808, 1160
BstSCI CCNGG 8 cut(s) 80, 81, 174, 295, 428, 650, 806, 1158
BstV1I GCAGC 1 cut(s) 23
BstV2I GAAGAC 1 cut(s) 617
BstX2I RGATCY 2 cut(s) 138, 696
BstXI CCANNNNNNTGG 2 cut(s) 700, 810
BstYI RGATCY 2 cut(s) 138, 696
BsuI GTATCC 1 cut(s) 158
BsuRI GGCC 5 cut(s) 80, 234, 300, 341, 728
BtgI CCRYGG 2 cut(s) 362, 509
BtgZI GCGATG 1 cut(s) 1151
BtrI CACGTC 1 cut(s) 766
BtsCI GGATG 5 cut(s) 372, 519, 668, 877, 1024
Cac8I GCNNGC 1 cut(s) 343
CfoI GCGC 1 cut(s) 661
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 3 cut(s) 79, 233, 726
Cfr9I CCCGGG 1 cut(s) 81
Csp6I GTAC 2 cut(s) 450, 1180
CspCI CAANNNNNGTGG 2 cut(s) 1198, 1233
CviAII CATG 5 cut(s) 559, 602, 786, 937, 1015
CviQI GTAC 2 cut(s) 450, 1180
DdeI CTNAG 1 cut(s) 777
DpnI GATC 5 cut(s) 140, 408, 698, 784, 1141
DpnII GATC 5 cut(s) 138, 406, 696, 782, 1139
EaeI YGGCCR 2 cut(s) 298, 339
Eam1104I CTCTTC 4 cut(s) 32, 48, 1060, 1104
EarI CTCTTC 4 cut(s) 32, 48, 1060, 1104
EciI GGCGGA 1 cut(s) 59
Eco24I GRGCYC 2 cut(s) 326, 613
Eco31I GGTCTC 1 cut(s) 370
Eco47III AGCGCT 1 cut(s) 660
Eco88I CYCGRG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 726
EcoRII CCWGG 5 cut(s) 174, 428, 650, 806, 1158
EcoT38I GRGCYC 2 cut(s) 326, 613
FaeI CATG 5 cut(s) 562, 605, 789, 940, 1018
FatI CATG 5 cut(s) 558, 601, 785, 936, 1014
FauI CCCGC 1 cut(s) 510
FauNDI CATATG 1 cut(s) 303
FblI GTMKAC 1 cut(s) 525
Fnu4HI GCNGC 3 cut(s) 12, 346, 570
FokI GGATG 5 cut(s) 379, 526, 655, 884, 1031
FriOI GRGCYC 2 cut(s) 326, 613
Fsp4HI GCNGC 3 cut(s) 12, 346, 570
FspBI CTAG 3 cut(s) 723, 792, 980
GlaI GCGC 1 cut(s) 660
GluI GCNGC 3 cut(s) 12, 346, 570
GsuI CTGGAG 1 cut(s) 916
HaeII RGCGCY 1 cut(s) 662
HaeIII GGCC 5 cut(s) 80, 234, 300, 341, 728
HapII CCGG 5 cut(s) 82, 297, 342, 482, 494
HhaI GCGC 1 cut(s) 661
Hin1II CATG 5 cut(s) 562, 605, 789, 940, 1018
Hin6I GCGC 1 cut(s) 659
HinP1I GCGC 1 cut(s) 659
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HinfI GANTC 4 cut(s) 110, 478, 719, 927
HpaII CCGG 5 cut(s) 82, 297, 342, 482, 494
HphI GGTGA 3 cut(s) 214, 764, 968
Hpy166II GTNNAC 4 cut(s) 526, 897, 959, 1110
Hpy188I TCNGA 3 cut(s) 136, 185, 211
Hpy188III TCNNGA 3 cut(s) 327, 482, 1137
Hpy8I GTNNAC 4 cut(s) 526, 897, 959, 1110
Hpy99I CGWCG 3 cut(s) 104, 530, 767
HpyAV CCTTC 1 cut(s) 216
HpyCH4III ACNGT 4 cut(s) 170, 530, 742, 881
HpyCH4IV ACGT 3 cut(s) 291, 713, 765
HpyCH4V TGCA 3 cut(s) 129, 680, 1087
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 734
HpyF3I CTNAG 1 cut(s) 777
HpySE526I ACGT 3 cut(s) 291, 713, 765
Hsp92II CATG 5 cut(s) 562, 605, 789, 940, 1018
HspAI GCGC 1 cut(s) 659
Kpn2I TCCGGA 1 cut(s) 481
KroI GCCGGC 1 cut(s) 341
KroNI GCCGGC 1 cut(s) 343
Kzo9I GATC 5 cut(s) 138, 406, 696, 782, 1139
LmnI GCTCC 2 cut(s) 8, 616
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 1 cut(s) 504
MaeI CTAG 3 cut(s) 723, 792, 980
MaeII ACGT 3 cut(s) 291, 713, 765
MaeIII GTNAC 1 cut(s) 703
MalI GATC 5 cut(s) 140, 408, 698, 784, 1141
MboI GATC 5 cut(s) 138, 406, 696, 782, 1139
MfeI CAATTG 1 cut(s) 630
MflI RGATCY 2 cut(s) 138, 696
MhlI GDGCHC 3 cut(s) 326, 613, 1051
MluCI AATT 6 cut(s) 330, 485, 630, 836, 964, 1118
MlyI GAGTC 2 cut(s) 713, 936
MmeI TCCRAC 3 cut(s) 267, 273, 840
MroI TCCGGA 1 cut(s) 481
MroNI GCCGGC 1 cut(s) 341
MroXI GAANNNNTTC 1 cut(s) 291
MseI TTAA 1 cut(s) 1008
MslI CAYNNNNRTG 1 cut(s) 808
MspA1I CMGCKG 1 cut(s) 1044
MspI CCGG 5 cut(s) 82, 297, 342, 482, 494
MspR9I CCNGG 8 cut(s) 82, 83, 176, 297, 430, 652, 808, 1160
MunI CAATTG 1 cut(s) 630
MvaI CCWGG 5 cut(s) 176, 430, 652, 808, 1160
MwoI GCNNNNNNNGC 2 cut(s) 77, 734
NaeI GCCGGC 1 cut(s) 343
NciI CCSGG 3 cut(s) 82, 83, 297
NdeI CATATG 1 cut(s) 303
NdeII GATC 5 cut(s) 138, 406, 696, 782, 1139
NgoMIV GCCGGC 1 cut(s) 341
NlaIII CATG 5 cut(s) 562, 605, 789, 940, 1018
NlaIV GGNNCC 5 cut(s) 235, 420, 492, 612, 691
NmuCI GTSAC 1 cut(s) 703
NspV TTCGAA 1 cut(s) 1116
OliI CACNNNNGTG 1 cut(s) 808
PasI CCCWGGG 1 cut(s) 1159
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PdiI GCCGGC 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 2 cut(s) 110, 478
PkrI GCNGC 3 cut(s) 13, 347, 571
PleI GAGTC 2 cut(s) 713, 935
PpsI GAGTC 2 cut(s) 713, 935
Psp6I CCWGG 5 cut(s) 174, 428, 650, 806, 1158
PspGI CCWGG 5 cut(s) 174, 428, 650, 806, 1158
PspN4I GGNNCC 5 cut(s) 235, 420, 492, 612, 691
PspPI GGNCC 3 cut(s) 79, 233, 726
PsuI RGATCY 2 cut(s) 138, 696
PvuII CAGCTG 1 cut(s) 1044
RsaI GTAC 2 cut(s) 451, 1181
RsaNI GTAC 2 cut(s) 450, 1180
RseI CAYNNNNRTG 1 cut(s) 808
SalI GTCGAC 1 cut(s) 524
SaqAI TTAA 1 cut(s) 1008
SatI GCNGC 3 cut(s) 12, 346, 570
Sau3AI GATC 5 cut(s) 138, 406, 696, 782, 1139
Sau96I GGNCC 3 cut(s) 79, 233, 726
SchI GAGTC 2 cut(s) 713, 936
ScrFI CCNGG 8 cut(s) 82, 83, 176, 297, 430, 652, 808, 1160
SduI GDGCHC 3 cut(s) 326, 613, 1051
SfaNI GCATC 1 cut(s) 504
SfuI TTCGAA 1 cut(s) 1116
SmaI CCCGGG 1 cut(s) 83
SmiMI CAYNNNNRTG 1 cut(s) 808
SmlI CTYRAG 1 cut(s) 1186
SmoI CTYRAG 1 cut(s) 1186
Sse9I AATT 6 cut(s) 330, 485, 630, 836, 964, 1118
SsiI CCGC 6 cut(s) 44, 345, 517, 569, 814, 977
SspMI CTAG 3 cut(s) 723, 792, 980
StyD4I CCNGG 8 cut(s) 80, 81, 174, 295, 428, 650, 806, 1158
TaaI ACNGT 4 cut(s) 170, 530, 742, 881
TaiI ACGT 3 cut(s) 294, 716, 768
TaqI TCGA 8 cut(s) 328, 409, 525, 762, 1063, 1116, 1130, 1176
TasI AATT 6 cut(s) 330, 485, 630, 836, 964, 1118
TatI WGTACW 1 cut(s) 1179
TauI GCSGC 2 cut(s) 348, 572
TfiI GAWTC 2 cut(s) 110, 478
Tru1I TTAA 1 cut(s) 1008
Tru9I TTAA 1 cut(s) 1008
TseFI GTSAC 1 cut(s) 703
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 703
TspDTI ATGAA 7 cut(s) 190, 424, 657, 809, 953, 1026, 1068
TspGWI ACGGA 2 cut(s) 351, 498
TspMI CCCGGG 1 cut(s) 81
XapI RAATTY 2 cut(s) 836, 1118
XcmI CCANNNNNNNNNTGG 1 cut(s) 308
XmaI CCCGGG 1 cut(s) 81
XmiI GTMKAC 1 cut(s) 525
XmnI GAANNNNTTC 1 cut(s) 291
XspI CTAG 3 cut(s) 723, 792, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.