Rroxscaffold_4G00303210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007529
Physical Location & Seq
Forward (+)
23594299 .. 23596437
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303210.1

Sequence Viewer

Length: 522 bp
ATGGCTATCGGTGGTACTGCGGCGCAGTGTGTGTCTATGGCGGGGCTCCTCCCGGTGTCCGATTGGGTGGGGCGGTCTCAGTGCTTCAAGACCCTCCCTTTCGAGGATGCTCTACAGTTTGGCTCATTGATCAAGGTGGCGATGTTGTGGTCGGCAACGGAGACGGCAAGAACGGAGCTGCAGACGGCGGGGTTGCTGGTGGTGGAGGTGTCGCTGGCTTGGGACCTATGGCTGCTGGCGCTCGGCTCCATGGTGGCTAGGATTTGCCATGGGCCATGGGCTTTTGCCACAAACCGAAACGTTAAGAATTACTTGCTAGCCCAAGTGTTTAATATGGCAGTGGCACAACTCTCGGCTCTTGCTCTGATTGCGTTTCTGGTTTCTCCGATCTTGGTGATGGTGATGGCAAATTCATACGAAGTTGACTGGTCAACTGAAATTGCTTCCAGTGGTTTTGCTCATGAAAACACTTTCTTTGCTGGAGATGTACTCAGTGAGCGATCTAGAACTTTGGCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

18.66

Weight (kDa)

5.02

Isoelectric Point (pI)

36.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 20, 41, 73, 188
AclI AACGTT 1 cut(s) 300
AcsI RAATTY 1 cut(s) 409
AfaI GTAC 2 cut(s) 16, 489
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 1 cut(s) 88
AluBI AGCT 2 cut(s) 178, 518
AluI AGCT 2 cut(s) 178, 518
Alw26I GTCTC 2 cut(s) 81, 155
AoxI GGCC 1 cut(s) 272
ApeKI GCWGC 3 cut(s) 178, 232, 515
ApoI RAATTY 1 cut(s) 409
AspLEI GCGC 2 cut(s) 25, 241
AspS9I GGNCC 2 cut(s) 223, 272
AsuC2I CCSGG 1 cut(s) 53
AsuHPI GGTGA 2 cut(s) 406, 412
AsuNHI GCTAGC 1 cut(s) 316
AvaII GGWCC 1 cut(s) 223
BanII GRGCYC 1 cut(s) 48
BbvI GCAGC 2 cut(s) 165, 219
BccI CCATC 2 cut(s) 391, 397
BceAI ACGGC 2 cut(s) 180, 201
BcgI CGANNNNNNTGC 2 cut(s) 333, 367
BclI TGATCA 1 cut(s) 129
BcnI CCSGG 1 cut(s) 53
BcoDI GTCTC 2 cut(s) 81, 155
BfaI CTAG 3 cut(s) 258, 317, 504
BfmI CTRYAG 2 cut(s) 113, 179
BfoI RGCGCY 1 cut(s) 242
BisI GCNGC 4 cut(s) 21, 179, 233, 516
BlsI GCNGC 4 cut(s) 22, 180, 234, 517
Bme1390I CCNGG 1 cut(s) 53
Bme18I GGWCC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 223, 272
BmiI GGNNCC 3 cut(s) 47, 224, 247
BmrFI CCNGG 1 cut(s) 53
BmsI GCATC 1 cut(s) 97
BmtI GCTAGC 1 cut(s) 320
BplI GAGNNNNNCTC 2 cut(s) 474, 506
BpmI CTGGAG 1 cut(s) 501
BpuMI CCSGG 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 81
BsaJI CCNNGG 3 cut(s) 249, 268, 275
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 2 cut(s) 431, 447
BseDI CCNNGG 3 cut(s) 249, 268, 275
BseGI GGATG 1 cut(s) 112
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMII CTCAG 2 cut(s) 92, 505
BseNI ACTGG 2 cut(s) 431, 447
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 2 cut(s) 165, 219
BshFI GGCC 1 cut(s) 274
BsiSI CCGG 1 cut(s) 53
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 2 cut(s) 81, 155
BsmBI CGTCTC 1 cut(s) 155
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 1 cut(s) 274
Bso31I GGTCTC 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 3 cut(s) 129, 387, 500
Bsp19I CCATGG 3 cut(s) 249, 268, 275
BspACI CCGC 4 cut(s) 20, 41, 73, 188
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 2 cut(s) 91, 504
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 3 cut(s) 47, 224, 247
BspMAI CTGCAG 1 cut(s) 183
BspOI GCTAGC 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 81
BsrI ACTGG 2 cut(s) 431, 447
BssECI CCNNGG 3 cut(s) 249, 268, 275
BssMI GATC 3 cut(s) 129, 387, 500
BssT1I CCWWGG 3 cut(s) 249, 268, 275
Bst4CI ACNGT 1 cut(s) 117
BstC8I GCNNGC 3 cut(s) 216, 237, 318
BstDEI CTNAG 3 cut(s) 78, 491, 519
BstDSI CCRYGG 3 cut(s) 249, 268, 275
BstF5I GGATG 1 cut(s) 112
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 2 cut(s) 25, 241
BstKTI GATC 3 cut(s) 132, 390, 503
BstMAI GTCTC 2 cut(s) 81, 155
BstMBI GATC 3 cut(s) 129, 387, 500
BstMWI GCNNNNNNNGC 2 cut(s) 238, 368
BstSCI CCNGG 1 cut(s) 51
BstSFI CTRYAG 2 cut(s) 113, 179
BstV1I GCAGC 2 cut(s) 165, 219
BsuRI GGCC 1 cut(s) 274
BtgI CCRYGG 3 cut(s) 249, 268, 275
BtgZI GCGATG 1 cut(s) 155
BtsCI GGATG 1 cut(s) 112
BtsI GCAGTG 2 cut(s) 32, 345
BtsIMutI CAGTG 5 cut(s) 32, 86, 345, 454, 499
Cac8I GCNNGC 3 cut(s) 216, 237, 318
CciI TCATGA 1 cut(s) 460
CfoI GCGC 2 cut(s) 25, 241
Cfr13I GGNCC 2 cut(s) 223, 272
Csp6I GTAC 2 cut(s) 15, 488
CviAII CATG 4 cut(s) 250, 269, 276, 461
CviQI GTAC 2 cut(s) 15, 488
DdeI CTNAG 3 cut(s) 78, 491, 519
DpnI GATC 3 cut(s) 131, 389, 502
DpnII GATC 3 cut(s) 129, 387, 500
Eco130I CCWWGG 3 cut(s) 249, 268, 275
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 223
EcoT14I CCWWGG 3 cut(s) 249, 268, 275
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 249, 268, 275
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 4 cut(s) 253, 272, 279, 464
FaiI YATR 8 cut(s) 38, 229, 251, 270, 277, 335, 415, 462
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FaqI GGGAC 1 cut(s) 236
FatI CATG 4 cut(s) 249, 268, 275, 460
FauI CCCGC 2 cut(s) 34, 181
FbaI TGATCA 1 cut(s) 129
Fnu4HI GCNGC 4 cut(s) 21, 179, 233, 516
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 4 cut(s) 21, 179, 233, 516
FspBI CTAG 3 cut(s) 258, 317, 504
GlaI GCGC 2 cut(s) 24, 240
GluI GCNGC 4 cut(s) 21, 179, 233, 516
GsuI CTGGAG 1 cut(s) 501
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 1 cut(s) 274
HapII CCGG 1 cut(s) 53
HhaI GCGC 2 cut(s) 25, 241
Hin1II CATG 4 cut(s) 253, 272, 279, 464
Hin6I GCGC 2 cut(s) 23, 239
HinP1I GCGC 2 cut(s) 23, 239
HincII GTYRAC 2 cut(s) 424, 432
HindII GTYRAC 2 cut(s) 424, 432
HpaII CCGG 1 cut(s) 53
HphI GGTGA 2 cut(s) 406, 412
Hpy166II GTNNAC 2 cut(s) 424, 432
Hpy188I TCNGA 3 cut(s) 61, 366, 387
Hpy188III TCNNGA 3 cut(s) 88, 461, 504
Hpy8I GTNNAC 2 cut(s) 424, 432
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 1 cut(s) 181
HpyF10VI GCNNNNNNNGC 2 cut(s) 238, 368
HpyF3I CTNAG 3 cut(s) 78, 491, 519
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 4 cut(s) 253, 272, 279, 464
HspAI GCGC 2 cut(s) 23, 239
Ksp22I TGATCA 1 cut(s) 129
Kzo9I GATC 3 cut(s) 129, 387, 500
LmnI GCTCC 3 cut(s) 51, 175, 251
LpnPI CCDG 8 cut(s) 66, 182, 200, 221, 362, 412, 460, 465
Lsp1109I GCAGC 2 cut(s) 165, 219
LweI GCATC 1 cut(s) 97
MaeI CTAG 3 cut(s) 258, 317, 504
MaeII ACGT 1 cut(s) 300
MalI GATC 3 cut(s) 131, 389, 502
MboI GATC 3 cut(s) 129, 387, 500
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 3 cut(s) 307, 409, 438
MnlI CCTC 4 cut(s) 59, 97, 104, 199
MseI TTAA 2 cut(s) 303, 330
MspI CCGG 1 cut(s) 53
MspR9I CCNGG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 238, 368
NciI CCSGG 1 cut(s) 53
NcoI CCATGG 3 cut(s) 249, 268, 275
NdeII GATC 3 cut(s) 129, 387, 500
NheI GCTAGC 1 cut(s) 316
NlaIII CATG 4 cut(s) 253, 272, 279, 464
NlaIV GGNNCC 3 cut(s) 47, 224, 247
NmeAIII GCCGAG 2 cut(s) 222, 332
PagI TCATGA 1 cut(s) 460
PkrI GCNGC 4 cut(s) 22, 180, 234, 517
PpuMI RGGWCCY 1 cut(s) 223
Psp1406I AACGTT 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 223
PspN4I GGNNCC 3 cut(s) 47, 224, 247
PspPI GGNCC 2 cut(s) 223, 272
PspPPI RGGWCCY 1 cut(s) 223
PstI CTGCAG 1 cut(s) 183
RsaI GTAC 2 cut(s) 16, 489
RsaNI GTAC 2 cut(s) 15, 488
SaqAI TTAA 2 cut(s) 303, 330
SatI GCNGC 4 cut(s) 21, 179, 233, 516
Sau3AI GATC 3 cut(s) 129, 387, 500
Sau96I GGNCC 2 cut(s) 223, 272
ScrFI CCNGG 1 cut(s) 53
SduI GDGCHC 1 cut(s) 48
SetI ASST 6 cut(s) 138, 180, 210, 228, 303, 520
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 2 cut(s) 113, 179
SinI GGWCC 1 cut(s) 223
Sse9I AATT 3 cut(s) 307, 409, 438
SsiI CCGC 4 cut(s) 20, 41, 73, 188
SspMI CTAG 3 cut(s) 258, 317, 504
StyD4I CCNGG 1 cut(s) 51
StyI CCWWGG 3 cut(s) 249, 268, 275
TaaI ACNGT 1 cut(s) 117
TaiI ACGT 1 cut(s) 303
TaqI TCGA 1 cut(s) 102
TasI AATT 3 cut(s) 307, 409, 438
TatI WGTACW 1 cut(s) 487
TauI GCSGC 1 cut(s) 23
Tru1I TTAA 2 cut(s) 303, 330
Tru9I TTAA 2 cut(s) 303, 330
TscAI CASTG 5 cut(s) 32, 86, 345, 454, 499
TseI GCWGC 3 cut(s) 178, 232, 515
TspDTI ATGAA 2 cut(s) 402, 477
TspGWI ACGGA 2 cut(s) 173, 188
TspRI CASTG 5 cut(s) 32, 86, 345, 454, 499
VpaK11BI GGWCC 1 cut(s) 223
XapI RAATTY 1 cut(s) 409
XbaI TCTAGA 1 cut(s) 503
XspI CTAG 3 cut(s) 258, 317, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.