Rroxscaffold_5G00347260

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005559
Physical Location & Seq
Forward (+)
18563901 .. 18571338
7438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00347260.1

Sequence Viewer

Length: 1068 bp
ATGGCCAAGTGTTTCTATCTTTACGAGCATCTATTTGATTCGAATGTGGCTTTTGAATATGAACACTTACCCCACTTTTGTCTCTTCTGTGGAAAATTTGATCATGTGAGGGCTGAGTGTGAAGAGAGACTGGCAGGGAGAATTACGGTGGAGAAGTATGGGAGGTGGAAGACCTTGTCTAAGAATGTATTCTCCATTGACCCTAATGGGCAACTGGTTGGGCGGTCATTTGGCTTGATTCCTAGTAAGTATAGCTGGTCTCTTAAAGCACCGGAGGCCCCTGTTTGTGGTTTTGTTAGGGAGAGGTCTGATGAAGCAAATGTAGAGGAGGATAGAGTTATGGGAGGAGTGGAATTTCTCAGTATGTCTAAAAGAAGACGGCTAATTTGGGAGCAACCAAATAATGAGGTGGAGATGAGAAGGAAGCAATTCCAAGTTGGACTAGAAGACCCAAGAAGCCCAAGTCAAATAGGAATTAAAAAGAGAGATACAAGGCAGAAACCTAGGACGTTTTTGGGGGACACAAGAGGGGCTGGAGAGCAGATTGGAACGCAAGAAGAGCAAGAGGATGACTTCTCATGGTTCAACCGTCAAAGTTACGAAGCTACACAATGGCCACGCCAAGCTGGCAACATTCCATCATCCTATGAAATCTTGATCGAGCCAAAGCAGCCATCACTTGAGGAGACATTACAAGAACTGATGCAAATTAATGTTATTAATCAACAAATGCTTCAAGACATTCAACAGACTTGCCCATCTATGCCAATTGAGCCAAAGAAGCCAACCCTTGAGGAAACCATGCAACAACTCGCACAAAATCAGATGGAGTTCCAAAAGGCTAATGTTCATATGTTTCGGGCAATTTATGAGACAAACAAAGAGCAAAAAGTAATTGCTGAAGCCATAGCCAAGTTGGAGGTACAAGTCGGTCACATTGCAGCTGCTTTGAATAAAGAAGAGGAAGGAAGAGTTCCCAGTCAAATAATTCCTCATCCAATGGAAGAATTTGAATTCATCTCCCCCACAATCATGCTCAATACCATGAGCAGGTCAACTCCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1

Protein Analysis

355

Amino Acids

41.11

Weight (kDa)

5.5

Isoelectric Point (pI)

63.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 14 - 41 5.7e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1041
AciI CCGC 1 cut(s) 223
AcoI YGGCCR 2 cut(s) 3, 614
AcsI RAATTY 4 cut(s) 95, 353, 1007, 1013
AcuI CTGAAG 1 cut(s) 921
AfaI GTAC 1 cut(s) 924
AfiI CCNNNNNNNGG 2 cut(s) 287, 1050
AgsI TTSAA 6 cut(s) 56, 586, 737, 746, 952, 1013
AluBI AGCT 4 cut(s) 255, 605, 626, 944
AluI AGCT 4 cut(s) 255, 605, 626, 944
Alw26I GTCTC 5 cut(s) 86, 121, 264, 680, 866
AoxI GGCC 3 cut(s) 3, 276, 614
ApeKI GCWGC 3 cut(s) 670, 941, 944
ApoI RAATTY 4 cut(s) 95, 353, 1007, 1013
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
AseI ATTAAT 2 cut(s) 711, 720
Asp700I GAANNNNTTC 2 cut(s) 188, 428
AspA2I CCTAGG 1 cut(s) 503
AspS9I GGNCC 1 cut(s) 277
AsuII TTCGAA 1 cut(s) 41
AvrII CCTAGG 1 cut(s) 503
BalI TGGCCA 2 cut(s) 5, 616
BbsI GAAGAC 3 cut(s) 176, 382, 453
BbvI GCAGC 3 cut(s) 682, 931, 953
BccI CCATC 4 cut(s) 646, 682, 766, 820
BceAI ACGGC 1 cut(s) 395
BclI TGATCA 1 cut(s) 100
BcoDI GTCTC 5 cut(s) 86, 121, 264, 680, 866
BfaI CTAG 3 cut(s) 243, 443, 504
BfuAI ACCTGC 1 cut(s) 1041
BglI GCCNNNNNGGC 1 cut(s) 627
BisI GCNGC 3 cut(s) 671, 942, 945
BlnI CCTAGG 1 cut(s) 503
BlsI GCNGC 3 cut(s) 672, 943, 946
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 1 cut(s) 279
BmrI ACTGGG 1 cut(s) 972
BmsI GCATC 2 cut(s) 37, 693
BmuI ACTGGG 1 cut(s) 972
BpiI GAAGAC 3 cut(s) 176, 382, 453
BpmI CTGGAG 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 41
BpuEI CTTGAG 2 cut(s) 701, 812
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 1 cut(s) 503
BsaWI WCCGGW 1 cut(s) 271
Bsc4I CCNNNNNNNGG 2 cut(s) 287, 1050
Bse1I ACTGG 3 cut(s) 135, 219, 978
Bse3DI GCAATG 1 cut(s) 936
BseDI CCNNGG 1 cut(s) 503
BseGI GGATG 3 cut(s) 574, 641, 994
BseLI CCNNNNNNNGG 2 cut(s) 287, 1050
BseMI GCAATG 1 cut(s) 936
BseMII CTCAG 2 cut(s) 105, 373
BseNI ACTGG 3 cut(s) 135, 219, 978
BseRI GAGGAG 3 cut(s) 341, 360, 698
BseXI GCAGC 3 cut(s) 682, 931, 953
BshFI GGCC 3 cut(s) 5, 278, 616
BsiSI CCGG 1 cut(s) 272
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 2 cut(s) 287, 1050
BsmAI GTCTC 5 cut(s) 86, 121, 264, 680, 866
BsmFI GGGAC 1 cut(s) 533
BsnI GGCC 3 cut(s) 5, 278, 616
Bso31I GGTCTC 1 cut(s) 264
Bsp119I TTCGAA 1 cut(s) 41
Bsp143I GATC 2 cut(s) 100, 657
BspACI CCGC 1 cut(s) 223
BspANI GGCC 3 cut(s) 5, 278, 616
BspCNI CTCAG 2 cut(s) 106, 372
BspLI GGNNCC 1 cut(s) 279
BspMI ACCTGC 1 cut(s) 1041
BspQI GCTCTTC 1 cut(s) 552
BspT104I TTCGAA 1 cut(s) 41
BspTNI GGTCTC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 936
BsrI ACTGG 3 cut(s) 135, 219, 978
BssECI CCNNGG 1 cut(s) 503
BssMI GATC 2 cut(s) 100, 657
BssT1I CCWWGG 1 cut(s) 503
Bst4CI ACNGT 2 cut(s) 148, 590
Bst6I CTCTTC 5 cut(s) 89, 117, 552, 954, 964
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 628
BstDEI CTNAG 3 cut(s) 114, 180, 359
BstF5I GGATG 3 cut(s) 574, 641, 994
BstKTI GATC 2 cut(s) 103, 660
BstMAI GTCTC 5 cut(s) 86, 121, 264, 680, 866
BstMBI GATC 2 cut(s) 100, 657
BstMWI GCNNNNNNNGC 6 cut(s) 275, 559, 627, 670, 772, 781
BstV1I GCAGC 3 cut(s) 682, 931, 953
BstV2I GAAGAC 3 cut(s) 176, 382, 453
BsuRI GGCC 3 cut(s) 5, 278, 616
BtsCI GGATG 3 cut(s) 574, 641, 994
BveI ACCTGC 1 cut(s) 1041
Cac8I GCNNGC 1 cut(s) 628
Cfr13I GGNCC 1 cut(s) 277
Csp6I GTAC 1 cut(s) 923
CviAII CATG 5 cut(s) 104, 579, 802, 1033, 1045
CviQI GTAC 1 cut(s) 923
DdeI CTNAG 3 cut(s) 114, 180, 359
DpnI GATC 2 cut(s) 102, 659
DpnII GATC 2 cut(s) 100, 657
EaeI YGGCCR 2 cut(s) 3, 614
Eam1104I CTCTTC 5 cut(s) 89, 117, 552, 954, 964
EarI CTCTTC 5 cut(s) 89, 117, 552, 954, 964
Eco130I CCWWGG 1 cut(s) 503
Eco31I GGTCTC 1 cut(s) 264
Eco57I CTGAAG 1 cut(s) 921
EcoO109I RGGNCCY 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 1013
EcoT14I CCWWGG 1 cut(s) 503
ErhI CCWWGG 1 cut(s) 503
FaeI CATG 5 cut(s) 107, 582, 805, 1036, 1048
FaqI GGGAC 1 cut(s) 533
FatI CATG 5 cut(s) 103, 578, 801, 1032, 1044
FauNDI CATATG 1 cut(s) 852
FbaI TGATCA 1 cut(s) 100
Fnu4HI GCNGC 3 cut(s) 671, 942, 945
FokI GGATG 3 cut(s) 581, 628, 981
Fsp4HI GCNGC 3 cut(s) 671, 942, 945
FspBI CTAG 3 cut(s) 243, 443, 504
GluI GCNGC 3 cut(s) 671, 942, 945
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 3 cut(s) 5, 278, 616
HapII CCGG 1 cut(s) 272
Hin1II CATG 5 cut(s) 107, 582, 805, 1036, 1048
HincII GTYRAC 1 cut(s) 1056
HindII GTYRAC 1 cut(s) 1056
HinfI GANTC 2 cut(s) 38, 238
HpaII CCGG 1 cut(s) 272
Hpy166II GTNNAC 1 cut(s) 1056
Hpy188I TCNGA 2 cut(s) 310, 825
Hpy188III TCNNGA 2 cut(s) 655, 737
Hpy8I GTNNAC 1 cut(s) 1056
HpyAV CCTTC 2 cut(s) 414, 959
HpyCH4III ACNGT 2 cut(s) 148, 590
HpyCH4IV ACGT 1 cut(s) 509
HpyCH4V TGCA 3 cut(s) 706, 805, 941
HpyF10VI GCNNNNNNNGC 6 cut(s) 275, 559, 627, 670, 772, 781
HpyF3I CTNAG 3 cut(s) 114, 180, 359
HpySE526I ACGT 1 cut(s) 509
Hsp92II CATG 5 cut(s) 107, 582, 805, 1036, 1048
Ksp22I TGATCA 1 cut(s) 100
Kzo9I GATC 2 cut(s) 100, 657
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 1 cut(s) 391
Lsp1109I GCAGC 3 cut(s) 682, 931, 953
LweI GCATC 2 cut(s) 37, 693
MaeI CTAG 3 cut(s) 243, 443, 504
MaeII ACGT 1 cut(s) 509
MaeIII GTNAC 2 cut(s) 596, 932
MalI GATC 2 cut(s) 102, 659
MboI GATC 2 cut(s) 100, 657
MboII GAAGA 9 cut(s) 76, 134, 181, 387, 458, 569, 971, 981, 1016
MfeI CAATTG 1 cut(s) 768
MlsI TGGCCA 2 cut(s) 5, 616
MluNI TGGCCA 2 cut(s) 5, 616
MmeI TCCRAC 2 cut(s) 418, 897
Mox20I TGGCCA 2 cut(s) 5, 616
MroXI GAANNNNTTC 2 cut(s) 188, 428
MscI TGGCCA 2 cut(s) 5, 616
MseI TTAA 4 cut(s) 264, 477, 711, 720
MslI CAYNNNNRTG 1 cut(s) 1031
Msp20I TGGCCA 2 cut(s) 5, 616
MspA1I CMGCKG 1 cut(s) 944
MspI CCGG 1 cut(s) 272
MunI CAATTG 1 cut(s) 768
MwoI GCNNNNNNNGC 6 cut(s) 275, 559, 627, 670, 772, 781
NdeI CATATG 1 cut(s) 852
NdeII GATC 2 cut(s) 100, 657
NlaIII CATG 5 cut(s) 107, 582, 805, 1036, 1048
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 932
NspV TTCGAA 1 cut(s) 41
PciSI GCTCTTC 1 cut(s) 552
PdmI GAANNNNTTC 2 cut(s) 188, 428
PfeI GAWTC 2 cut(s) 38, 238
PflFI GACNNNGTC 1 cut(s) 175
PkrI GCNGC 3 cut(s) 672, 943, 946
PshBI ATTAAT 2 cut(s) 711, 720
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 277
PsyI GACNNNGTC 1 cut(s) 175
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 1 cut(s) 924
RsaNI GTAC 1 cut(s) 923
RseI CAYNNNNRTG 1 cut(s) 1031
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 4 cut(s) 264, 477, 711, 720
SatI GCNGC 3 cut(s) 671, 942, 945
Sau3AI GATC 2 cut(s) 100, 657
Sau96I GGNCC 1 cut(s) 277
SfaNI GCATC 2 cut(s) 37, 693
SfuI TTCGAA 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 1031
SmlI CTYRAG 2 cut(s) 680, 791
SmoI CTYRAG 2 cut(s) 680, 791
SsiI CCGC 1 cut(s) 223
SspMI CTAG 3 cut(s) 243, 443, 504
StyI CCWWGG 1 cut(s) 503
TaaI ACNGT 2 cut(s) 148, 590
TaiI ACGT 1 cut(s) 512
TaqI TCGA 2 cut(s) 41, 660
TaqII GACCGA 1 cut(s) 920
TfiI GAWTC 2 cut(s) 38, 238
Tru1I TTAA 4 cut(s) 264, 477, 711, 720
Tru9I TTAA 4 cut(s) 264, 477, 711, 720
TseFI GTSAC 1 cut(s) 932
TseI GCWGC 3 cut(s) 670, 941, 944
Tsp45I GTSAC 1 cut(s) 932
TspDTI ATGAA 5 cut(s) 75, 327, 663, 839, 1006
Tth111I GACNNNGTC 1 cut(s) 175
VspI ATTAAT 2 cut(s) 711, 720
XapI RAATTY 4 cut(s) 95, 353, 1007, 1013
XcmI CCANNNNNNNNNTGG 1 cut(s) 913
XmaJI CCTAGG 1 cut(s) 503
XmnI GAANNNNTTC 2 cut(s) 188, 428
XspI CTAG 3 cut(s) 243, 443, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.