Rh4BG132000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005559
Physical Location & Seq
Reverse (-)
22199015 .. 22200126
1112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG132000.1

Sequence Viewer

Length: 759 bp
ATGCAATGTCCGAAAATAATTCATATTCATGGGATGATCCTTCATGGCCAACAAGAACCATCTAATAGCAATTTGGGATGGGGAGATCATTCTAACAACTCATGGTCTGAAAATCCATATTATGAAACCGCACAATGGTCATATCCATCTAGCAATTTCCCATCATCTTATCAAGCTCCAATTTATCAACAAGAAGATCCTACTGTTAAGGAGACGGTGGTTGAGCTATTCAAGAGATCAGAAAAATTTGATCACGAGGTTGAAAGGCTTAAACATGATCAATCAAAGCTTGAAGAAAGGCAATCCAAGCTTGCACAAGCACTACTGGAAATTGAACTTCATATATCGCAGCTTGCTAATGCTTGGGAGTTAGAGGACGAAAGGCATGCTAGTGGAAGAGAAGAAAATTTTGATCGTAAGCCCGATGAGGAAGATGACTATTTTGAGCTACATATTATTGATGATCAAGCTACCGAGAGCACCTATCTTCCTTCTTCTACTAAAGTTCCTATGGAGCTATGTGCTAACAAGCCAAAGAATGAAGAACCTGAATTCAAAGATGTGAGATCTCATACATTGGATGCTCTTGATGAAGTCACCACTGTGGTGGTTGATGCACCAAATTTTCAAGCATCTACGGAGTACCAAATTTGCCTCCAAGACTTGAGCTCATCTGTGTTTTCAAGCCTCCCTCAAATTCTAAGGATGAGTTCCTCCCACAATCACTTCAACCTCCTTGGCCACCTCCTCTGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

29.06

Weight (kDa)

4.77

Isoelectric Point (pI)

62.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 129
AclWI GGATC 2 cut(s) 31, 191
AcoI YGGCCR 2 cut(s) 46, 739
AcsI RAATTY 6 cut(s) 245, 406, 551, 622, 648, 696
AfaI GTAC 1 cut(s) 644
AfiI CCNNNNNNNGG 1 cut(s) 135
AgsI TTSAA 8 cut(s) 232, 263, 293, 335, 556, 629, 684, 730
AleI CACNNNNGTG 2 cut(s) 602, 605
AluBI AGCT 9 cut(s) 176, 226, 289, 310, 352, 448, 470, 517, 669
AluI AGCT 9 cut(s) 176, 226, 289, 310, 352, 448, 470, 517, 669
Alw21I GWGCWC 2 cut(s) 482, 671
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 2 cut(s) 31, 191
AoxI GGCC 2 cut(s) 46, 739
ApeKI GCWGC 1 cut(s) 349
ApoI RAATTY 6 cut(s) 245, 406, 551, 622, 648, 696
AsuHPI GGTGA 1 cut(s) 589
BalI TGGCCA 2 cut(s) 48, 741
BanII GRGCYC 1 cut(s) 671
BauI CACGAG 1 cut(s) 254
Bbv12I GWGCWC 2 cut(s) 482, 671
BbvI GCAGC 1 cut(s) 361
BccI CCATC 4 cut(s) 67, 72, 154, 169
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BclI TGATCA 3 cut(s) 250, 277, 463
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 2 cut(s) 150, 390
BglII AGATCT 1 cut(s) 566
BisI GCNGC 1 cut(s) 350
BlsI GCNGC 1 cut(s) 351
BmsI GCATC 3 cut(s) 571, 604, 641
BpuEI CTTGAG 1 cut(s) 685
BsaJI CCNNGG 1 cut(s) 736
Bsc4I CCNNNNNNNGG 1 cut(s) 135
Bse1I ACTGG 1 cut(s) 330
Bse3DI GCAATG 1 cut(s) 11
BseDI CCNNGG 1 cut(s) 736
BseGI GGATG 4 cut(s) 39, 83, 586, 711
BseLI CCNNNNNNNGG 1 cut(s) 135
BseMI GCAATG 1 cut(s) 11
BseNI ACTGG 1 cut(s) 330
BseRI GAGGAG 1 cut(s) 737
BseXI GCAGC 1 cut(s) 361
BshFI GGCC 2 cut(s) 48, 741
BsiHKAI GWGCWC 2 cut(s) 482, 671
BslI CCNNNNNNNGG 1 cut(s) 135
BsmAI GTCTC 1 cut(s) 206
BsmBI CGTCTC 1 cut(s) 206
BsnI GGCC 2 cut(s) 48, 741
Bsp1286I GDGCHC 2 cut(s) 482, 671
Bsp143I GATC 9 cut(s) 36, 85, 196, 236, 250, 277, 412, 463, 566
BspACI CCGC 1 cut(s) 129
BspANI GGCC 2 cut(s) 48, 741
BspPI GGATC 2 cut(s) 31, 191
BsrDI GCAATG 1 cut(s) 11
BsrI ACTGG 1 cut(s) 330
BssECI CCNNGG 1 cut(s) 736
BssMI GATC 9 cut(s) 36, 85, 196, 236, 250, 277, 412, 463, 566
BssSI CACGAG 1 cut(s) 254
BssT1I CCWWGG 1 cut(s) 736
Bst2BI CACGAG 1 cut(s) 254
Bst4CI ACNGT 3 cut(s) 205, 217, 604
Bst6I CTCTTC 1 cut(s) 391
BstC8I GCNNGC 3 cut(s) 312, 354, 387
BstDEI CTNAG 1 cut(s) 701
BstF5I GGATG 4 cut(s) 39, 83, 586, 711
BstKTI GATC 9 cut(s) 39, 88, 199, 239, 253, 280, 415, 466, 569
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 9 cut(s) 36, 85, 196, 236, 250, 277, 412, 463, 566
BstMWI GCNNNNNNNGC 1 cut(s) 307
BstNSI RCATGY 1 cut(s) 389
BstV1I GCAGC 1 cut(s) 361
BstX2I RGATCY 2 cut(s) 196, 566
BstXI CCANNNNNNTGG 1 cut(s) 607
BstYI RGATCY 2 cut(s) 196, 566
BsuRI GGCC 2 cut(s) 48, 741
BtsCI GGATG 4 cut(s) 39, 83, 586, 711
BtsIMutI CAGTG 1 cut(s) 600
Cac8I GCNNGC 3 cut(s) 312, 354, 387
Csp6I GTAC 1 cut(s) 643
CviAII CATG 5 cut(s) 29, 44, 102, 275, 386
CviQI GTAC 1 cut(s) 643
DdeI CTNAG 1 cut(s) 701
DpnI GATC 9 cut(s) 38, 87, 198, 238, 252, 279, 414, 465, 568
DpnII GATC 9 cut(s) 36, 85, 196, 236, 250, 277, 412, 463, 566
EaeI YGGCCR 2 cut(s) 46, 739
Eam1104I CTCTTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 391
Ecl136II GAGCTC 1 cut(s) 669
Eco130I CCWWGG 1 cut(s) 736
Eco24I GRGCYC 1 cut(s) 671
Eco53kI GAGCTC 1 cut(s) 669
EcoICRI GAGCTC 1 cut(s) 669
EcoRI GAATTC 1 cut(s) 551
EcoT14I CCWWGG 1 cut(s) 736
EcoT38I GRGCYC 1 cut(s) 671
ErhI CCWWGG 1 cut(s) 736
Esp3I CGTCTC 1 cut(s) 206
FaeI CATG 5 cut(s) 32, 47, 105, 278, 389
FatI CATG 5 cut(s) 28, 43, 101, 274, 385
FbaI TGATCA 3 cut(s) 250, 277, 463
Fnu4HI GCNGC 1 cut(s) 350
FokI GGATG 4 cut(s) 46, 90, 593, 718
FriOI GRGCYC 1 cut(s) 671
Fsp4HI GCNGC 1 cut(s) 350
FspBI CTAG 2 cut(s) 150, 390
GluI GCNGC 1 cut(s) 350
HaeIII GGCC 2 cut(s) 48, 741
Hin1II CATG 5 cut(s) 32, 47, 105, 278, 389
HindIII AAGCTT 2 cut(s) 287, 308
HphI GGTGA 1 cut(s) 589
Hpy188I TCNGA 3 cut(s) 12, 109, 241
Hpy188III TCNNGA 3 cut(s) 232, 254, 587
HpyAV CCTTC 2 cut(s) 50, 501
HpyCH4III ACNGT 3 cut(s) 205, 217, 604
HpyCH4V TGCA 4 cut(s) 4, 314, 617, 753
HpyF10VI GCNNNNNNNGC 1 cut(s) 307
HpyF3I CTNAG 1 cut(s) 701
Hsp92II CATG 5 cut(s) 32, 47, 105, 278, 389
Ksp22I TGATCA 3 cut(s) 250, 277, 463
Kzo9I GATC 9 cut(s) 36, 85, 196, 236, 250, 277, 412, 463, 566
LmnI GCTCC 2 cut(s) 181, 514
LpnPI CCDG 2 cut(s) 311, 561
Lsp1109I GCAGC 1 cut(s) 361
LweI GCATC 3 cut(s) 571, 604, 641
MaeI CTAG 2 cut(s) 150, 390
MaeIII GTNAC 1 cut(s) 595
MalI GATC 9 cut(s) 38, 87, 198, 238, 252, 279, 414, 465, 568
MboI GATC 9 cut(s) 36, 85, 196, 236, 250, 277, 412, 463, 566
MboII GAAGA 8 cut(s) 206, 305, 408, 413, 443, 479, 486, 554
MfeI CAATTG 1 cut(s) 754
MflI RGATCY 2 cut(s) 196, 566
MhlI GDGCHC 2 cut(s) 482, 671
MlsI TGGCCA 2 cut(s) 48, 741
MluNI TGGCCA 2 cut(s) 48, 741
Mox20I TGGCCA 2 cut(s) 48, 741
MscI TGGCCA 2 cut(s) 48, 741
MseI TTAA 2 cut(s) 207, 270
MslI CAYNNNNRTG 4 cut(s) 27, 390, 602, 605
Msp20I TGGCCA 2 cut(s) 48, 741
MunI CAATTG 1 cut(s) 754
MwoI GCNNNNNNNGC 1 cut(s) 307
NdeII GATC 9 cut(s) 36, 85, 196, 236, 250, 277, 412, 463, 566
NlaIII CATG 5 cut(s) 32, 47, 105, 278, 389
NmuCI GTSAC 1 cut(s) 595
NspI RCATGY 1 cut(s) 389
OliI CACNNNNGTG 2 cut(s) 602, 605
PaeI GCATGC 1 cut(s) 389
PkrI GCNGC 1 cut(s) 351
Psp124BI GAGCTC 1 cut(s) 671
PsuI RGATCY 2 cut(s) 196, 566
RsaI GTAC 1 cut(s) 644
RsaNI GTAC 1 cut(s) 643
RseI CAYNNNNRTG 4 cut(s) 27, 390, 602, 605
SacI GAGCTC 1 cut(s) 671
SaqAI TTAA 2 cut(s) 207, 270
SatI GCNGC 1 cut(s) 350
Sau3AI GATC 9 cut(s) 36, 85, 196, 236, 250, 277, 412, 463, 566
SduI GDGCHC 2 cut(s) 482, 671
SfaNI GCATC 3 cut(s) 571, 604, 641
SmiMI CAYNNNNRTG 4 cut(s) 27, 390, 602, 605
SmlI CTYRAG 1 cut(s) 664
SmoI CTYRAG 1 cut(s) 664
SphI GCATGC 1 cut(s) 389
SsiI CCGC 1 cut(s) 129
SspMI CTAG 2 cut(s) 150, 390
SstI GAGCTC 1 cut(s) 671
StyI CCWWGG 1 cut(s) 736
TaaI ACNGT 3 cut(s) 205, 217, 604
Tru1I TTAA 2 cut(s) 207, 270
Tru9I TTAA 2 cut(s) 207, 270
TscAI CASTG 1 cut(s) 607
TseFI GTSAC 1 cut(s) 595
TseI GCWGC 1 cut(s) 349
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 7 cut(s) 11, 17, 32, 138, 329, 555, 606
TspGWI ACGGA 1 cut(s) 653
TspRI CASTG 1 cut(s) 607
XapI RAATTY 6 cut(s) 245, 406, 551, 622, 648, 696
XceI RCATGY 1 cut(s) 389
XspI CTAG 2 cut(s) 150, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.