Rroxscaffold_7G00185450

Peptidyl-prolyl cis-trans isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003751
Physical Location & Seq
Forward (+)
24832937 .. 24836550
3614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00185450.1

Sequence Viewer

Length: 1485 bp
ATGGACGACGACTTCGATATGGCAGGCCCCGGCGGCGAGATGATGGACGACTTTGACCTCCCCGACGAGGGCCCCGTTCTCAAGGTCGGCGAGGAGAAGGAGATCGGAAAGCAGGGCTTGAAGAAGAAGCTCCTCAAGGAAGGTGAAAGCTGGGAAACGCCCAAGAGCGGCGACGAGGTTGAAGTACACTACACTGGGACTTTGCTGGACGGGACTCAGTTCGATTCGAGCCGCGATAGGGGGACGCCGTTCAAGTTCACTCTCGGTCAAGGAAAAGTGATTAAAGGATGGGATGAAGGTATTAAAACAATGAAGAAAGGTGAGAATGCCCTTTTCACCATTCCTCCTGATATGGCTTATGGTGAATCTGGATCTGCCCCAACCATCCCTCCAAATGCAACGTTGCAGTTCGATGTTGATCTGCTATCGTGGACCAGTGTAAACGACATAACCAAGGATGTTAAATTTGAGGCCCAGCTCGAAGATAAAACACTTGTAGCAAAATCTGACACCGTGGAATTCACTGTCAAAGAGGATGGATTTGGGGACAAGGGTAAGCCTGCATCTGGTGATGAGGGTGCTGTTCCTCCAAATGCAACTCTTCATATTACTCTAGAGTTGGTATCATGGAGAACAGTCTCAGAACTGACAGATGACAAGAAAGTCATTAAGAAGATCCTAAAAGAAGGGGAAGGGTATGAGCGTCCAAATGAAGGAGCTGTTGTTACAGTGAAATTGATTGGAAAGTTGCAAGATGGTAAGGAATTTTTAAAGAAAGGTCATGGTGAAGGAGAAGAACCATTTGAGTTCAAGACAGATGAGGAGCAAGTCATTGAGGGGCTTGATAGAGCTGTAGTGACAATGAAGAAGGGTGAGATAGCTCTTGTGATTATAGCCCCTGAGTATGGATTTGGCTCGTCAGAGTCCCAGCAGGAGTTGGCAGTGGTGCCTCCTAATTCCACCCTCTATTATGAGGTCGAGCTTGTATCTTTTGTCAAGGACAAGGAATCATGGGACATGAATACTCCGGAGAAAATTGAGGCTGCTGGTAAGAAGAAGGAAGAAGGGAATGCTTTCTTTAAGGCTGGTAAATATGCAAAAGCTTCCAAGAGATATGAGAAGGCTAAGGTGTTGAAGGTTGCTTGCAATCTCAATGATGCTGCTTGCAAATTGAAGCTGAAAGAGTACAAACAGGCTGAAAAGCTGTGTACCAAGGTGTTGGAGCTTGAGGGTAAGAATGTAAAGGCCCTGTACAGAAGAGCTCAGGCATATATCCAGCTGGCTGATTTGGATTTGGCTGAGCTTGACATCAAGAAAGCTCTTGAAATAGACCCTAACAACAGGGATGTGAAGCTGGAGTACAAAACTTTGAAGGAGAAGATGAAAGAATTCAATAAAAAGGAGGCAAAGTTCTATGGCAACATGTTTGCTAAACTGACTAAAAATTCCAATAAGGATGCTGAACCAATGAGCGTAGACAGCTAG

Protein Analysis

494

Amino Acids

54.94

Weight (kDa)

5.09

Isoelectric Point (pI)

25.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKBP_C PF00254 52 - 142 7.7e-38 FKBP-type peptidyl-prolyl cis-trans isomerase
FKBP_C PF00254 233 - 329 5.5e-24 FKBP-type peptidyl-prolyl cis-trans isomerase
TPR_19 PF14559 391 - 451 6e-06 Tetratricopeptide repeat
TPR_1 PF00515 414 - 447 3.6e-07 Tetratricopeptide repeat
TPR_8 PF13181 415 - 447 4.3e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 662
AccB1I GGYRCC 1 cut(s) 946
AccBSI CCGCTC 1 cut(s) 168
AccI GTMKAC 1 cut(s) 1476
AccII CGCG 1 cut(s) 234
AccIII TCCGGA 1 cut(s) 1027
AciI CCGC 3 cut(s) 33, 168, 232
AclI AACGTT 1 cut(s) 401
AclWI GGATC 2 cut(s) 379, 670
AcsI RAATTY 5 cut(s) 464, 518, 764, 1388, 1444
AcyI GRCGYC 1 cut(s) 245
AfaI GTAC 5 cut(s) 186, 1187, 1210, 1253, 1361
AfiI CCNNNNNNNGG 7 cut(s) 67, 68, 167, 238, 566, 713, 905
AflIII ACRYGT 1 cut(s) 1422
AgsI TTSAA 9 cut(s) 121, 182, 253, 811, 1135, 1174, 1325, 1372, 1393
AloI GAACNNNNNNTCC 2 cut(s) 1394, 1426
Alw21I GWGCWC 1 cut(s) 1264
Alw26I GTCTC 1 cut(s) 643
AlwI GGATC 2 cut(s) 379, 670
Aor13HI TCCGGA 1 cut(s) 1027
AoxI GGCC 4 cut(s) 25, 70, 471, 1245
ApaI GGGCCC 1 cut(s) 74
ApeKI GCWGC 2 cut(s) 1043, 1160
ApoI RAATTY 5 cut(s) 464, 518, 764, 1388, 1444
ArsI GACNNNNNNTTYG 1 cut(s) 28
Asp700I GAANNNNTTC 2 cut(s) 1073, 1388
AspS9I GGNCC 6 cut(s) 26, 70, 71, 432, 472, 1246
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 7 cut(s) 155, 328, 332, 374, 581, 797, 884
AvaII GGWCC 1 cut(s) 432
BaeGI GKGCMC 1 cut(s) 74
BanI GGYRCC 1 cut(s) 946
BanII GRGCYC 2 cut(s) 74, 1264
BarI GAAGNNNNNNTAC 2 cut(s) 1343, 1375
Bbv12I GWGCWC 1 cut(s) 1264
BbvI GCAGC 2 cut(s) 1030, 1147
BccI CCATC 5 cut(s) 37, 282, 392, 530, 749
BceAI ACGGC 1 cut(s) 232
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 643
BfaI CTAG 2 cut(s) 614, 1483
BfmI CTRYAG 1 cut(s) 852
BglI GCCNNNNNGGC 1 cut(s) 33
BisI GCNGC 5 cut(s) 34, 169, 232, 1044, 1161
BlpI GCTNAGC 1 cut(s) 1299
BlsI GCNGC 5 cut(s) 35, 170, 233, 1045, 1162
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 432
BmgT120I GGNCC 6 cut(s) 26, 70, 71, 432, 472, 1246
BmiI GGNNCC 4 cut(s) 28, 72, 73, 948
BmrFI CCNGG 1 cut(s) 30
BmrI ACTGGG 1 cut(s) 204
BmsI GCATC 3 cut(s) 572, 1147, 1447
BmuI ACTGGG 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 1376
Bpu10I CCTNAGC 2 cut(s) 1125, 1263
Bpu1102I GCTNAGC 1 cut(s) 1299
BpuEI CTTGAG 3 cut(s) 65, 119, 1247
BpuMI CCSGG 1 cut(s) 30
BsaBI GATNNNNATC 1 cut(s) 417
BsaHI GRCGYC 1 cut(s) 245
BsaJI CCNNGG 4 cut(s) 28, 453, 513, 1212
BsaWI WCCGGW 1 cut(s) 1027
BsaXI ACNNNNNCTCC 8 cut(s) 328, 358, 373, 403, 708, 738, 1394, 1424
Bsc4I CCNNNNNNNGG 7 cut(s) 67, 68, 167, 238, 566, 713, 905
Bse1I ACTGG 2 cut(s) 199, 435
Bse8I GATNNNNATC 1 cut(s) 417
BseAI TCCGGA 1 cut(s) 1027
BseDI CCNNGG 4 cut(s) 28, 453, 513, 1212
BseGI GGATG 7 cut(s) 293, 298, 384, 463, 541, 1351, 1462
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 7 cut(s) 67, 68, 167, 238, 566, 713, 905
BseMII CTCAG 5 cut(s) 230, 654, 891, 1277, 1290
BseNI ACTGG 2 cut(s) 199, 435
BseRI GAGGAG 3 cut(s) 107, 122, 836
BseSI GKGCMC 1 cut(s) 74
BseXI GCAGC 2 cut(s) 1030, 1147
BseYI CCCAGC 3 cut(s) 150, 474, 927
Bsh1236I CGCG 1 cut(s) 234
BshFI GGCC 4 cut(s) 27, 72, 473, 1247
BshNI GGYRCC 1 cut(s) 946
BsiHKAI GWGCWC 1 cut(s) 1264
BsiSI CCGG 2 cut(s) 30, 1028
BslFI GGGAC 6 cut(s) 211, 226, 256, 560, 910, 1028
BslI CCNNNNNNNGG 7 cut(s) 67, 68, 167, 238, 566, 713, 905
BsmAI GTCTC 1 cut(s) 643
BsmFI GGGAC 6 cut(s) 211, 226, 256, 560, 910, 1028
BsmI GAATGC 2 cut(s) 331, 1075
BsnI GGCC 4 cut(s) 27, 72, 473, 1247
Bsp120I GGGCCC 1 cut(s) 70
Bsp1286I GDGCHC 2 cut(s) 74, 1264
Bsp13I TCCGGA 1 cut(s) 1027
Bsp1407I TGTACA 1 cut(s) 1251
Bsp143I GATC 4 cut(s) 102, 371, 418, 675
Bsp1720I GCTNAGC 1 cut(s) 1299
BspACI CCGC 3 cut(s) 33, 168, 232
BspANI GGCC 4 cut(s) 27, 72, 473, 1247
BspCNI CTCAG 5 cut(s) 229, 653, 892, 1276, 1291
BspEI TCCGGA 1 cut(s) 1027
BspFNI CGCG 1 cut(s) 234
BspLI GGNNCC 4 cut(s) 28, 72, 73, 948
BspPI GGATC 2 cut(s) 379, 670
BspQI GCTCTTC 1 cut(s) 1252
BspT107I GGYRCC 1 cut(s) 946
BsrBI CCGCTC 1 cut(s) 168
BsrGI TGTACA 1 cut(s) 1251
BsrI ACTGG 2 cut(s) 199, 435
BssECI CCNNGG 4 cut(s) 28, 453, 513, 1212
BssMI GATC 4 cut(s) 102, 371, 418, 675
BssNI GRCGYC 1 cut(s) 245
BssT1I CCWWGG 2 cut(s) 453, 1212
Bst4CI ACNGT 4 cut(s) 514, 526, 637, 730
Bst6I CTCTTC 2 cut(s) 606, 1252
BstACI GRCGYC 1 cut(s) 245
BstAUI TGTACA 1 cut(s) 1251
BstC8I GCNNGC 5 cut(s) 25, 561, 1144, 1165, 1281
BstDEI CTNAG 6 cut(s) 216, 640, 900, 1125, 1263, 1299
BstDSI CCRYGG 1 cut(s) 513
BstF5I GGATG 7 cut(s) 293, 298, 384, 463, 541, 1351, 1462
BstFNI CGCG 1 cut(s) 234
BstKTI GATC 4 cut(s) 105, 374, 421, 678
BstMAI GTCTC 1 cut(s) 643
BstMBI GATC 4 cut(s) 102, 371, 418, 675
BstMWI GCNNNNNNNGC 2 cut(s) 33, 1479
BstNSI RCATGY 1 cut(s) 1426
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 1 cut(s) 852
BstSLI GKGCMC 1 cut(s) 74
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 2 cut(s) 1030, 1147
BstX2I RGATCY 2 cut(s) 371, 675
BstXI CCANNNNNNTGG 1 cut(s) 1219
BstYI RGATCY 2 cut(s) 371, 675
BsuRI GGCC 4 cut(s) 27, 72, 473, 1247
BtgI CCRYGG 1 cut(s) 513
BtsCI GGATG 7 cut(s) 293, 298, 384, 463, 541, 1351, 1462
BtsI GCAGTG 1 cut(s) 948
BtsIMutI CAGTG 5 cut(s) 192, 442, 522, 735, 948
Cac8I GCNNGC 5 cut(s) 25, 561, 1144, 1165, 1281
Cfr13I GGNCC 6 cut(s) 26, 70, 71, 432, 472, 1246
CseI GACGC 2 cut(s) 253, 692
Csp6I GTAC 5 cut(s) 185, 1186, 1209, 1252, 1360
CviAII CATG 5 cut(s) 627, 782, 1011, 1018, 1423
CviQI GTAC 5 cut(s) 185, 1186, 1209, 1252, 1360
DdeI CTNAG 6 cut(s) 216, 640, 900, 1125, 1263, 1299
DpnI GATC 4 cut(s) 104, 373, 420, 677
DpnII GATC 4 cut(s) 102, 371, 418, 675
DraI TTTAAA 1 cut(s) 771
DrdI GACNNNNNNGTC 1 cut(s) 662
DseDI GACNNNNNNGTC 1 cut(s) 662
Eam1104I CTCTTC 2 cut(s) 606, 1252
EarI CTCTTC 2 cut(s) 606, 1252
Ecl136II GAGCTC 1 cut(s) 1262
Eco130I CCWWGG 2 cut(s) 453, 1212
Eco24I GRGCYC 2 cut(s) 74, 1264
Eco47I GGWCC 1 cut(s) 432
Eco53kI GAGCTC 1 cut(s) 1262
EcoICRI GAGCTC 1 cut(s) 1262
EcoO109I RGGNCCY 4 cut(s) 26, 70, 71, 1246
EcoRI GAATTC 2 cut(s) 518, 1388
EcoT14I CCWWGG 2 cut(s) 453, 1212
EcoT38I GRGCYC 2 cut(s) 74, 1264
ErhI CCWWGG 2 cut(s) 453, 1212
FaeI CATG 5 cut(s) 630, 785, 1014, 1021, 1426
FalI AAGNNNNNCTT 2 cut(s) 101, 133
FaqI GGGAC 6 cut(s) 211, 226, 256, 560, 910, 1028
FatI CATG 5 cut(s) 626, 781, 1010, 1017, 1422
FblI GTMKAC 1 cut(s) 1476
Fnu4HI GCNGC 5 cut(s) 34, 169, 232, 1044, 1161
FokI GGATG 7 cut(s) 300, 305, 371, 470, 548, 1358, 1469
FriOI GRGCYC 2 cut(s) 74, 1264
Fsp4HI GCNGC 5 cut(s) 34, 169, 232, 1044, 1161
FspBI CTAG 2 cut(s) 614, 1483
GluI GCNGC 5 cut(s) 34, 169, 232, 1044, 1161
GsaI CCCAGC 3 cut(s) 154, 478, 931
GsuI CTGGAG 1 cut(s) 1376
HaeIII GGCC 4 cut(s) 27, 72, 473, 1247
HapII CCGG 2 cut(s) 30, 1028
HgaI GACGC 2 cut(s) 253, 692
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 5 cut(s) 630, 785, 1014, 1021, 1426
HindIII AAGCTT 1 cut(s) 1101
HinfI GANTC 5 cut(s) 214, 224, 365, 923, 1007
HpaII CCGG 2 cut(s) 30, 1028
HphI GGTGA 7 cut(s) 155, 328, 332, 374, 581, 797, 884
Hpy166II GTNNAC 6 cut(s) 187, 258, 432, 442, 1209, 1477
Hpy188I TCNGA 4 cut(s) 107, 508, 643, 922
Hpy188III TCNNGA 7 cut(s) 347, 369, 614, 811, 1028, 1312, 1322
Hpy8I GTNNAC 6 cut(s) 187, 258, 432, 442, 1209, 1477
Hpy99I CGWCG 3 cut(s) 11, 68, 176
HpyCH4III ACNGT 4 cut(s) 514, 526, 637, 730
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 8 cut(s) 398, 406, 563, 596, 751, 1097, 1146, 1167
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 1479
HpyF3I CTNAG 6 cut(s) 216, 640, 900, 1125, 1263, 1299
HpySE526I ACGT 1 cut(s) 401
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 5 cut(s) 630, 785, 1014, 1021, 1426
Kpn2I TCCGGA 1 cut(s) 1027
Kzo9I GATC 4 cut(s) 102, 371, 418, 675
LguI GCTCTTC 1 cut(s) 1252
LmnI GCTCC 4 cut(s) 135, 716, 823, 1222
Lsp1109I GCAGC 2 cut(s) 1030, 1147
LweI GCATC 3 cut(s) 572, 1147, 1447
MaeI CTAG 2 cut(s) 614, 1483
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 2 cut(s) 724, 856
MalI GATC 4 cut(s) 104, 373, 420, 677
MbiI CCGCTC 1 cut(s) 168
MboI GATC 4 cut(s) 102, 371, 418, 675
MflI RGATCY 2 cut(s) 371, 675
MhlI GDGCHC 2 cut(s) 74, 1264
MluCI AATT 9 cut(s) 464, 518, 734, 764, 955, 1035, 1169, 1388, 1444
MlyI GAGTC 2 cut(s) 208, 932
MmeI TCCRAC 1 cut(s) 1200
MroI TCCGGA 1 cut(s) 1027
MroXI GAANNNNTTC 2 cut(s) 1073, 1388
MseI TTAA 6 cut(s) 282, 303, 462, 669, 770, 1080
MspA1I CMGCKG 1 cut(s) 1279
MspI CCGG 2 cut(s) 30, 1028
MspR9I CCNGG 1 cut(s) 30
Mva1269I GAATGC 2 cut(s) 331, 1075
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 2 cut(s) 33, 1479
NciI CCSGG 1 cut(s) 30
NdeII GATC 4 cut(s) 102, 371, 418, 675
NlaIII CATG 5 cut(s) 630, 785, 1014, 1021, 1426
NlaIV GGNNCC 4 cut(s) 28, 72, 73, 948
NmuCI GTSAC 1 cut(s) 856
NspI RCATGY 1 cut(s) 1426
PciI ACATGT 1 cut(s) 1422
PciSI GCTCTTC 1 cut(s) 1252
PcsI WCGNNNNNNNCGW 2 cut(s) 12, 72
PctI GAATGC 2 cut(s) 331, 1075
PdmI GAANNNNTTC 2 cut(s) 1073, 1388
PfeI GAWTC 3 cut(s) 224, 365, 1007
PkrI GCNGC 5 cut(s) 35, 170, 233, 1045, 1162
PleI GAGTC 2 cut(s) 208, 931
PpsI GAGTC 2 cut(s) 208, 931
PscI ACATGT 1 cut(s) 1422
Psp124BI GAGCTC 1 cut(s) 1264
Psp1406I AACGTT 1 cut(s) 401
PspFI CCCAGC 3 cut(s) 150, 474, 927
PspN4I GGNNCC 4 cut(s) 28, 72, 73, 948
PspOMI GGGCCC 1 cut(s) 70
PspPI GGNCC 6 cut(s) 26, 70, 71, 432, 472, 1246
PsuI RGATCY 2 cut(s) 371, 675
PvuII CAGCTG 1 cut(s) 1279
RsaI GTAC 5 cut(s) 186, 1187, 1210, 1253, 1361
RsaNI GTAC 5 cut(s) 185, 1186, 1209, 1252, 1360
SacI GAGCTC 1 cut(s) 1264
SapI GCTCTTC 1 cut(s) 1252
SaqAI TTAA 6 cut(s) 282, 303, 462, 669, 770, 1080
SatI GCNGC 5 cut(s) 34, 169, 232, 1044, 1161
Sau3AI GATC 4 cut(s) 102, 371, 418, 675
Sau96I GGNCC 6 cut(s) 26, 70, 71, 432, 472, 1246
SchI GAGTC 2 cut(s) 208, 932
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 2 cut(s) 74, 1264
SfaNI GCATC 3 cut(s) 572, 1147, 1447
SfcI CTRYAG 1 cut(s) 852
SinI GGWCC 1 cut(s) 432
SmlI CTYRAG 3 cut(s) 80, 134, 1226
SmoI CTYRAG 3 cut(s) 80, 134, 1226
Sse9I AATT 9 cut(s) 464, 518, 734, 764, 955, 1035, 1169, 1388, 1444
SsiI CCGC 3 cut(s) 33, 168, 232
SspMI CTAG 2 cut(s) 614, 1483
SstI GAGCTC 1 cut(s) 1264
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 2 cut(s) 453, 1212
TaaI ACNGT 4 cut(s) 514, 526, 637, 730
TaiI ACGT 1 cut(s) 404
TaqI TCGA 6 cut(s) 15, 222, 227, 411, 480, 978
TaqII GACCGA 1 cut(s) 254
TasI AATT 9 cut(s) 464, 518, 734, 764, 955, 1035, 1169, 1388, 1444
TatI WGTACW 4 cut(s) 184, 1185, 1251, 1359
TauI GCSGC 3 cut(s) 36, 171, 234
TfiI GAWTC 3 cut(s) 224, 365, 1007
Tru1I TTAA 6 cut(s) 282, 303, 462, 669, 770, 1080
Tru9I TTAA 6 cut(s) 282, 303, 462, 669, 770, 1080
TscAI CASTG 5 cut(s) 199, 442, 529, 735, 948
TseFI GTSAC 1 cut(s) 856
TseI GCWGC 2 cut(s) 1043, 1160
Tsp45I GTSAC 1 cut(s) 856
TspDTI ATGAA 7 cut(s) 309, 326, 593, 726, 878, 1034, 1397
TspRI CASTG 5 cut(s) 199, 442, 529, 735, 948
VpaK11BI GGWCC 1 cut(s) 432
XapI RAATTY 5 cut(s) 464, 518, 764, 1388, 1444
XbaI TCTAGA 1 cut(s) 613
XceI RCATGY 1 cut(s) 1426
XmiI GTMKAC 1 cut(s) 1476
XmnI GAANNNNTTC 2 cut(s) 1073, 1388
XspI CTAG 2 cut(s) 614, 1483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.