Rorug07G0086300

Peptidyl-prolyl cis-trans isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003751
Physical Location & Seq
Reverse (-)
6774677 .. 6775794
1118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0086300.1

Sequence Viewer

Length: 684 bp
ATGAGACCACCACCGAGAATACTACTAGAGGCGGCAGTAGCAGCAGTAGCCGAGTTCGGCGGCACAGTCCACTTCTCTCCACCACCACTCTCCACCTCGCCACCTCCGACGCCACCGCCATGCGTGGCTCACGCCTGCCGCTGGTGGCCCTACTCCGGCTCTAAAGAGTTCGAGGCCAACGCAGCCATGGTCCTCATCATCCTCCTCTCGGCTCTCATCTGCGCCCTCGGCCTCAACGCCGCCATCCGCTGCTTCCTCCGCGGCACTCACCTAGTGGAGGGGCAGCTCGACCGCCGCAGCAGCAACCCTACCCAGACGCATCACGAGTTGGCTCGCGTGCAGCTCAAGACCAATGCGGCCGCTGCTCCCTGCTTTGATGGGGGACCCACGCTGGTGTACTCGCCGGAGATGAAGGCGGAGCTGGCGGGGGCAGCGGCGGAGTGCGCCATTTGCTTGTCGGAGTTCGTGGATGGGGAGGGGATAAGGGTGTTGGAGAGGTGTAAGCATGGGTTCCACGCCCACTGTATACAAGCCTGGCTGTCTTCTCACTCTTCTTGTCCAACGTGCCGTCGCACGTGCCTCCCTCCCTCCTCCCCTCCTCCGCCTCCTCCTCCGCCGCCGCCGCCGCAGCAATCCACCGAGGTGGAGGACCAACCGCCCTTGCCGGAAAGTATTGCTCCGTGA

Protein Analysis

227

Amino Acids

24.17

Weight (kDa)

6.29

Isoelectric Point (pI)

66.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 147 - 190 3.1e-14 Ring finger domain
zf-RING_11 PF17123 147 - 176 1.3e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 147 - 189 5e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 148 - 189 3.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 162 - 190 1.5e-07 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 163 - 192 1e-05 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 526
AccII CGCG 2 cut(s) 261, 336
AcoI YGGCCR 1 cut(s) 357
AcvI CACGTG 1 cut(s) 576
AcyI GRCGYC 1 cut(s) 110
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 1 cut(s) 398
AfiI CCNNNNNNNGG 4 cut(s) 141, 155, 208, 277
AjnI CCWGG 1 cut(s) 533
AleI CACNNNNGTG 2 cut(s) 392, 641
AluBI AGCT 3 cut(s) 286, 343, 421
AluI AGCT 3 cut(s) 286, 343, 421
AoxI GGCC 4 cut(s) 146, 174, 229, 357
AspLEI GCGC 2 cut(s) 224, 446
AspS9I GGNCC 4 cut(s) 147, 190, 383, 649
AsuHPI GGTGA 1 cut(s) 260
AvaII GGWCC 3 cut(s) 190, 383, 649
BauI CACGAG 1 cut(s) 323
BbrPI CACGTG 1 cut(s) 576
BbsI GAAGAC 1 cut(s) 534
BccI CCATC 3 cut(s) 251, 371, 464
BceAI ACGGC 1 cut(s) 552
BciT130I CCWGG 1 cut(s) 535
BfaI CTAG 2 cut(s) 26, 272
Bme1390I CCNGG 1 cut(s) 535
Bme18I GGWCC 3 cut(s) 190, 383, 649
BmgT120I GGNCC 4 cut(s) 147, 190, 383, 649
BmiI GGNNCC 3 cut(s) 384, 385, 512
BmrFI CCNGG 1 cut(s) 535
BmsI GCATC 1 cut(s) 328
BpiI GAAGAC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 329
BsaAI YACGTR 1 cut(s) 576
BsaHI GRCGYC 1 cut(s) 110
BsaJI CCNNGG 4 cut(s) 186, 226, 259, 639
Bsc4I CCNNNNNNNGG 4 cut(s) 141, 155, 208, 277
BseBI CCWGG 1 cut(s) 535
BseDI CCNNGG 4 cut(s) 186, 226, 259, 639
BseGI GGATG 3 cut(s) 198, 243, 475
BseLI CCNNNNNNNGG 4 cut(s) 141, 155, 208, 277
BseRI GAGGAG 5 cut(s) 194, 580, 588, 597, 600
BseX3I CGGCCG 1 cut(s) 357
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 2 cut(s) 261, 336
Bsh1285I CGRYCG 2 cut(s) 292, 360
BshFI GGCC 4 cut(s) 148, 176, 231, 359
BsiEI CGRYCG 2 cut(s) 292, 360
BsiSI CCGG 3 cut(s) 156, 404, 665
BslFI GGGAC 1 cut(s) 396
BslI CCNNNNNNNGG 4 cut(s) 141, 155, 208, 277
BsmFI GGGAC 1 cut(s) 396
BsnI GGCC 4 cut(s) 148, 176, 231, 359
Bsp19I CCATGG 1 cut(s) 186
BspANI GGCC 4 cut(s) 148, 176, 231, 359
BspFNI CGCG 2 cut(s) 261, 336
BspLI GGNNCC 3 cut(s) 384, 385, 512
BssECI CCNNGG 4 cut(s) 186, 226, 259, 639
BssNAI GTATAC 1 cut(s) 527
BssNI GRCGYC 1 cut(s) 110
BssSI CACGAG 1 cut(s) 323
BssT1I CCWWGG 1 cut(s) 186
Bst1107I GTATAC 1 cut(s) 527
Bst2BI CACGAG 1 cut(s) 323
Bst2UI CCWGG 1 cut(s) 535
Bst4CI ACNGT 2 cut(s) 67, 524
Bst6I CTCTTC 1 cut(s) 556
BstACI GRCGYC 1 cut(s) 110
BstBAI YACGTR 1 cut(s) 576
BstC8I GCNNGC 4 cut(s) 136, 334, 338, 423
BstDSI CCRYGG 2 cut(s) 186, 259
BstENI CCTNNNNNAGG 1 cut(s) 275
BstF5I GGATG 3 cut(s) 198, 243, 475
BstFNI CGCG 2 cut(s) 261, 336
BstHHI GCGC 2 cut(s) 224, 446
BstMCI CGRYCG 2 cut(s) 292, 360
BstNI CCWGG 1 cut(s) 535
BstSCI CCNGG 1 cut(s) 533
BstUI CGCG 2 cut(s) 261, 336
BstV2I GAAGAC 1 cut(s) 534
BstXI CCANNNNNNTGG 1 cut(s) 643
BstZ17I GTATAC 1 cut(s) 527
BstZI CGGCCG 1 cut(s) 357
BsuRI GGCC 4 cut(s) 148, 176, 231, 359
BtgI CCRYGG 2 cut(s) 186, 259
BtsCI GGATG 3 cut(s) 198, 243, 475
BtsIMutI CAGTG 1 cut(s) 520
Cac8I GCNNGC 4 cut(s) 136, 334, 338, 423
CciNI GCGGCCGC 1 cut(s) 357
CfoI GCGC 2 cut(s) 224, 446
Cfr13I GGNCC 4 cut(s) 147, 190, 383, 649
Cfr42I CCGCGG 1 cut(s) 262
CseI GACGC 2 cut(s) 118, 325
Csp6I GTAC 1 cut(s) 397
CviAII CATG 3 cut(s) 120, 187, 506
CviQI GTAC 1 cut(s) 397
DraIII CACNNNGTG 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 357
EagI CGGCCG 1 cut(s) 357
Eam1104I CTCTTC 1 cut(s) 556
EarI CTCTTC 1 cut(s) 556
EciI GGCGGA 4 cut(s) 431, 452, 591, 603
EclXI CGGCCG 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 186
Eco47I GGWCC 3 cut(s) 190, 383, 649
Eco52I CGGCCG 1 cut(s) 357
Eco72I CACGTG 1 cut(s) 576
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 533
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 3 cut(s) 123, 190, 509
FaiI YATR 4 cut(s) 121, 188, 507, 527
FaqI GGGAC 1 cut(s) 396
FatI CATG 3 cut(s) 119, 186, 505
FauI CCCGC 1 cut(s) 418
FblI GTMKAC 1 cut(s) 526
FokI GGATG 3 cut(s) 185, 230, 482
FspBI CTAG 2 cut(s) 26, 272
GlaI GCGC 2 cut(s) 223, 445
HaeIII GGCC 4 cut(s) 148, 176, 231, 359
HapII CCGG 3 cut(s) 156, 404, 665
HgaI GACGC 2 cut(s) 118, 325
HhaI GCGC 2 cut(s) 224, 446
Hin1I GRCGYC 1 cut(s) 110
Hin1II CATG 3 cut(s) 123, 190, 509
Hin6I GCGC 2 cut(s) 222, 444
HinP1I GCGC 2 cut(s) 222, 444
HpaII CCGG 3 cut(s) 156, 404, 665
HphI GGTGA 1 cut(s) 260
Hpy166II GTNNAC 3 cut(s) 70, 397, 527
Hpy188I TCNGA 2 cut(s) 108, 460
Hpy188III TCNNGA 2 cut(s) 323, 346
Hpy8I GTNNAC 3 cut(s) 70, 397, 527
Hpy99I CGWCG 2 cut(s) 112, 573
HpyAV CCTTC 1 cut(s) 406
HpyCH4III ACNGT 2 cut(s) 67, 524
HpyCH4IV ACGT 2 cut(s) 563, 575
HpyCH4V TGCA 1 cut(s) 340
HpySE526I ACGT 2 cut(s) 563, 575
Hsp92I GRCGYC 1 cut(s) 110
Hsp92II CATG 3 cut(s) 123, 190, 509
HspAI GCGC 2 cut(s) 222, 444
KflI GGGWCCC 1 cut(s) 383
KspI CCGCGG 1 cut(s) 262
LmnI GCTCC 3 cut(s) 370, 418, 682
LweI GCATC 1 cut(s) 328
MaeI CTAG 2 cut(s) 26, 272
MaeII ACGT 2 cut(s) 563, 575
MboII GAAGA 2 cut(s) 534, 543
MmeI TCCRAC 4 cut(s) 131, 438, 471, 584
MslI CAYNNNNRTG 3 cut(s) 118, 392, 641
MspA1I CMGCKG 5 cut(s) 141, 249, 261, 362, 434
MspI CCGG 3 cut(s) 156, 404, 665
MspR9I CCNGG 1 cut(s) 535
MvaI CCWGG 1 cut(s) 535
MvnI CGCG 2 cut(s) 261, 336
NcoI CCATGG 1 cut(s) 186
NlaIII CATG 3 cut(s) 123, 190, 509
NlaIV GGNNCC 3 cut(s) 384, 385, 512
NmeAIII GCCGAG 3 cut(s) 76, 188, 207
NotI GCGGCCGC 1 cut(s) 357
OliI CACNNNNGTG 2 cut(s) 392, 641
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PmaCI CACGTG 1 cut(s) 576
PmlI CACGTG 1 cut(s) 576
Ppu21I YACGTR 1 cut(s) 576
PpuMI RGGWCCY 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 533
PspCI CACGTG 1 cut(s) 576
PspGI CCWGG 1 cut(s) 533
PspN4I GGNNCC 3 cut(s) 384, 385, 512
PspPI GGNCC 4 cut(s) 147, 190, 383, 649
PspPPI RGGWCCY 1 cut(s) 383
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
RseI CAYNNNNRTG 3 cut(s) 118, 392, 641
SacII CCGCGG 1 cut(s) 262
Sau96I GGNCC 4 cut(s) 147, 190, 383, 649
ScrFI CCNGG 1 cut(s) 535
SfaNI GCATC 1 cut(s) 328
Sfr303I CCGCGG 1 cut(s) 262
SgrBI CCGCGG 1 cut(s) 262
SinI GGWCC 3 cut(s) 190, 383, 649
SmiMI CAYNNNNRTG 3 cut(s) 118, 392, 641
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
SspMI CTAG 2 cut(s) 26, 272
StyD4I CCNGG 1 cut(s) 533
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 2 cut(s) 67, 524
TaiI ACGT 2 cut(s) 566, 578
TaqI TCGA 2 cut(s) 171, 288
TatI WGTACW 1 cut(s) 396
TscAI CASTG 1 cut(s) 527
TspDTI ATGAA 1 cut(s) 425
TspGWI ACGGA 1 cut(s) 669
TspRI CASTG 1 cut(s) 527
VpaK11BI GGWCC 3 cut(s) 190, 383, 649
XagI CCTNNNNNAGG 1 cut(s) 275
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 526
XspI CTAG 2 cut(s) 26, 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.