Rorug01G0040800

two-component response regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002461
Physical Location & Seq
Forward (+)
6676940 .. 6686710
9771 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0040800.1

Sequence Viewer

Length: 915 bp
ATGCTCCAACTGTGCATTGCAGCACAGTGCCTGGCCACTTTGACAGCTTCGGGGTTCGAACCTAGTTCTTCTTCTTTCTTCTTCCGCTTCTTCACTCCCAAAATCCAAAGCATAACAGAACCACCGCCGAGCTCTCTCTCCGAGCCCAAATCACCAAACCCATTGCGATTCCAGTCCCGTAGCCGATCAGTTCAACACCGCGCCTCGCATTTCGTCGCCGCACAATCTCATCGCCGAGCTTGGTTTCGAGTTGCGCCTCGCATTTCAGCCATTTCTGTGCCAGTAGATAAAGGTGTCAGGCTTAGTGATGCTTCAAAGAAGTTAGGGGAGAAGTTCGCTACAGGAGCTTTTGTTGTTAAGGTTATTCATGAAAATCTGGAAATCATCAAACTAGATGGAGACAGTGTGCGGTCAACATTCTTTAGTGACCAAGATCTAAGAGGTTGTGGTGTGAGTGAATCCACAAGGAAACAATTATGTTTCAGATGTAGAGGGCTAAGGGACACAAATGTTCAAAGAGTGGAATCCCATCAGATTATTCCGGAGGATAACTCTCCGTGGAAACTTATTTGGAACTTCCAGCTTCCTCCCAAACTGAAGACATTCACATGGATTGTTTGTCATGATAAAATCTTGACAAATGTTCAAAGAGCCAAAAGGAATTTGACCAATGACAACTCTTGTCCTTTATGCCATCAAGCTCCTGAGACTCTTGCTCACCTATTTCGAGATTGCCTTGCTGTTCAAGTGATATGGAATTCTTTCAATATTCCATCTTGTGTGGATAATACTTCTCAGATGAATTGGAATGGTTGGCTTATGGCTCATCTTCTGTGCAAGGCTTACTTGGAAAATTTGGATTCTCTTGGCAGATCTCGGAAGATTAAAACTGGGCATGATTCTGGTGCCTGCTAG

Protein Analysis

304

Amino Acids

34.33

Weight (kDa)

9.46

Isoelectric Point (pI)

51.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 182 - 252 4.2e-18 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 905
AccII CGCG 1 cut(s) 201
AccIII TCCGGA 1 cut(s) 541
AciI CCGC 5 cut(s) 85, 125, 199, 219, 409
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 3 cut(s) 661, 757, 853
AcuI CTGAAG 1 cut(s) 617
AgsI TTSAA 6 cut(s) 194, 315, 515, 647, 746, 766
AjnI CCWGG 1 cut(s) 30
AluBI AGCT 6 cut(s) 47, 132, 239, 347, 583, 701
AluI AGCT 6 cut(s) 47, 132, 239, 347, 583, 701
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 2 cut(s) 393, 701
AlwNI CAGNNNCTG 1 cut(s) 31
Aor13HI TCCGGA 1 cut(s) 541
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 1 cut(s) 20
ApoI RAATTY 3 cut(s) 661, 757, 853
Asp700I GAANNNNTTC 2 cut(s) 602, 761
AspLEI GCGC 2 cut(s) 203, 256
AsuHPI GGTGA 2 cut(s) 144, 710
AsuII TTCGAA 1 cut(s) 57
BalI TGGCCA 1 cut(s) 35
BanI GGYRCC 1 cut(s) 905
BanII GRGCYC 2 cut(s) 134, 147
BbsI GAAGAC 1 cut(s) 605
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 1 cut(s) 32
BccI CCATC 4 cut(s) 389, 537, 702, 781
BciT130I CCWGG 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 393, 701
BfaI CTAG 3 cut(s) 63, 392, 913
BfmI CTRYAG 1 cut(s) 339
BglII AGATCT 2 cut(s) 433, 872
BisI GCNGC 2 cut(s) 21, 219
BlsI GCNGC 2 cut(s) 22, 220
Bme1390I CCNGG 1 cut(s) 32
BmiI GGNNCC 1 cut(s) 907
BmrFI CCNGG 1 cut(s) 32
BmrI ACTGGG 1 cut(s) 900
BmsI GCATC 1 cut(s) 298
BmuI ACTGGG 1 cut(s) 900
BpiI GAAGAC 1 cut(s) 605
BplI GAGNNNNNCTC 2 cut(s) 536, 568
Bpu10I CCTNAGC 1 cut(s) 497
Bpu14I TTCGAA 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 557
BsaWI WCCGGW 1 cut(s) 541
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 390, 420
Bse1I ACTGG 3 cut(s) 172, 281, 895
Bse3DI GCAATG 2 cut(s) 15, 161
BseAI TCCGGA 1 cut(s) 541
BseBI CCWGG 1 cut(s) 32
BseDI CCNNGG 1 cut(s) 557
BseMI GCAATG 2 cut(s) 15, 161
BseMII CTCAG 2 cut(s) 696, 809
BseNI ACTGG 3 cut(s) 172, 281, 895
BseXI GCAGC 1 cut(s) 32
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 1 cut(s) 35
BshNI GGYRCC 1 cut(s) 905
BsiHKAI GWGCWC 1 cut(s) 134
BsiSI CCGG 1 cut(s) 542
BslFI GGGAC 2 cut(s) 160, 515
BsmAI GTCTC 2 cut(s) 393, 701
BsmFI GGGAC 2 cut(s) 160, 515
BsnI GGCC 1 cut(s) 35
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 134, 147
Bsp13I TCCGGA 1 cut(s) 541
Bsp143I GATC 3 cut(s) 185, 433, 872
BspACI CCGC 5 cut(s) 85, 125, 199, 219, 409
BspANI GGCC 1 cut(s) 35
BspCNI CTCAG 2 cut(s) 697, 808
BspEI TCCGGA 1 cut(s) 541
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 2 cut(s) 367, 622
BspLI GGNNCC 1 cut(s) 907
BspT104I TTCGAA 1 cut(s) 57
BspT107I GGYRCC 1 cut(s) 905
BsrDI GCAATG 2 cut(s) 15, 161
BsrI ACTGG 3 cut(s) 172, 281, 895
BssECI CCNNGG 1 cut(s) 557
BssMI GATC 3 cut(s) 185, 433, 872
Bst2UI CCWGG 1 cut(s) 32
Bst4CI ACNGT 3 cut(s) 12, 27, 404
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 1 cut(s) 910
BstDEI CTNAG 5 cut(s) 302, 437, 497, 705, 795
BstDSI CCRYGG 1 cut(s) 557
BstFNI CGCG 1 cut(s) 201
BstHHI GCGC 2 cut(s) 203, 256
BstKTI GATC 3 cut(s) 188, 436, 875
BstMAI GTCTC 2 cut(s) 393, 701
BstMBI GATC 3 cut(s) 185, 433, 872
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 30
BstSFI CTRYAG 1 cut(s) 339
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 32
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 2 cut(s) 433, 872
BstYI RGATCY 2 cut(s) 433, 872
BsuRI GGCC 1 cut(s) 35
BtgI CCRYGG 1 cut(s) 557
BtgZI GCGATG 1 cut(s) 215
BtsIMutI CAGTG 2 cut(s) 32, 409
Cac8I GCNNGC 1 cut(s) 910
CaiI CAGNNNCTG 1 cut(s) 31
CciI TCATGA 2 cut(s) 367, 622
CfoI GCGC 2 cut(s) 203, 256
CviAII CATG 4 cut(s) 368, 609, 623, 896
DdeI CTNAG 5 cut(s) 302, 437, 497, 705, 795
DpnI GATC 3 cut(s) 187, 435, 874
DpnII GATC 3 cut(s) 185, 433, 872
EaeI YGGCCR 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 132
Eco24I GRGCYC 2 cut(s) 134, 147
Eco53kI GAGCTC 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 617
EcoICRI GAGCTC 1 cut(s) 132
EcoRI GAATTC 1 cut(s) 757
EcoRII CCWGG 1 cut(s) 30
EcoT38I GRGCYC 2 cut(s) 134, 147
FaeI CATG 4 cut(s) 371, 612, 626, 899
FaiI YATR 9 cut(s) 113, 369, 478, 610, 624, 691, 754, 821, 897
FalI AAGNNNNNCTT 2 cut(s) 830, 862
FaqI GGGAC 2 cut(s) 160, 515
FatI CATG 4 cut(s) 367, 608, 622, 895
Fnu4HI GCNGC 2 cut(s) 21, 219
FriOI GRGCYC 2 cut(s) 134, 147
Fsp4HI GCNGC 2 cut(s) 21, 219
FspBI CTAG 3 cut(s) 63, 392, 913
GlaI GCGC 2 cut(s) 202, 255
GluI GCNGC 2 cut(s) 21, 219
HaeIII GGCC 1 cut(s) 35
HapII CCGG 1 cut(s) 542
HhaI GCGC 2 cut(s) 203, 256
Hin1II CATG 4 cut(s) 371, 612, 626, 899
Hin6I GCGC 2 cut(s) 201, 254
HinP1I GCGC 2 cut(s) 201, 254
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 6 cut(s) 168, 458, 524, 709, 860, 899
HpaII CCGG 1 cut(s) 542
HphI GGTGA 2 cut(s) 144, 710
Hpy166II GTNNAC 1 cut(s) 414
Hpy188I TCNGA 5 cut(s) 142, 485, 534, 798, 879
Hpy188III TCNNGA 7 cut(s) 368, 377, 542, 623, 634, 704, 728
Hpy8I GTNNAC 1 cut(s) 414
Hpy99I CGWCG 1 cut(s) 218
HpyCH4III ACNGT 3 cut(s) 12, 27, 404
HpyCH4V TGCA 3 cut(s) 15, 20, 837
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 5 cut(s) 302, 437, 497, 705, 795
Hsp92II CATG 4 cut(s) 371, 612, 626, 899
HspAI GCGC 2 cut(s) 201, 254
Kpn2I TCCGGA 1 cut(s) 541
Kzo9I GATC 3 cut(s) 185, 433, 872
LmnI GCTCC 3 cut(s) 9, 344, 706
Lsp1109I GCAGC 1 cut(s) 32
LweI GCATC 1 cut(s) 298
MaeI CTAG 3 cut(s) 63, 392, 913
MaeIII GTNAC 1 cut(s) 425
MalI GATC 3 cut(s) 187, 435, 874
MboI GATC 3 cut(s) 185, 433, 872
MboII GAAGA 8 cut(s) 60, 63, 70, 73, 82, 610, 821, 892
MflI RGATCY 2 cut(s) 433, 872
MhlI GDGCHC 2 cut(s) 134, 147
MlsI TGGCCA 1 cut(s) 35
MluCI AATT 5 cut(s) 473, 661, 757, 802, 853
MluNI TGGCCA 1 cut(s) 35
MlyI GAGTC 1 cut(s) 703
MmeI TCCRAC 1 cut(s) 31
MnlI CCTC 6 cut(s) 214, 267, 434, 485, 538, 597
Mox20I TGGCCA 1 cut(s) 35
MroI TCCGGA 1 cut(s) 541
MroXI GAANNNNTTC 2 cut(s) 602, 761
MscI TGGCCA 1 cut(s) 35
MseI TTAA 2 cut(s) 357, 885
MslI CAYNNNNRTG 2 cut(s) 275, 607
Msp20I TGGCCA 1 cut(s) 35
MspI CCGG 1 cut(s) 542
MspR9I CCNGG 1 cut(s) 32
MvaI CCWGG 1 cut(s) 32
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 1 cut(s) 344
NdeII GATC 3 cut(s) 185, 433, 872
NlaIII CATG 4 cut(s) 371, 612, 626, 899
NlaIV GGNNCC 1 cut(s) 907
NmeAIII GCCGAG 2 cut(s) 153, 260
NmuCI GTSAC 1 cut(s) 425
NspV TTCGAA 1 cut(s) 57
PagI TCATGA 2 cut(s) 367, 622
PdmI GAANNNNTTC 2 cut(s) 602, 761
PfeI GAWTC 5 cut(s) 168, 458, 524, 860, 899
PkrI GCNGC 2 cut(s) 22, 220
PleI GAGTC 1 cut(s) 703
PpsI GAGTC 1 cut(s) 703
Psp124BI GAGCTC 1 cut(s) 134
Psp6I CCWGG 1 cut(s) 30
PspGI CCWGG 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 907
PstNI CAGNNNCTG 1 cut(s) 31
PsuI RGATCY 2 cut(s) 433, 872
RseI CAYNNNNRTG 2 cut(s) 275, 607
SacI GAGCTC 1 cut(s) 134
SaqAI TTAA 2 cut(s) 357, 885
SatI GCNGC 2 cut(s) 21, 219
Sau3AI GATC 3 cut(s) 185, 433, 872
SchI GAGTC 1 cut(s) 703
ScrFI CCNGG 1 cut(s) 32
SduI GDGCHC 2 cut(s) 134, 147
SfaNI GCATC 1 cut(s) 298
SfcI CTRYAG 1 cut(s) 339
SfuI TTCGAA 1 cut(s) 57
SmiMI CAYNNNNRTG 2 cut(s) 275, 607
Sse9I AATT 5 cut(s) 473, 661, 757, 802, 853
SsiI CCGC 5 cut(s) 85, 125, 199, 219, 409
SspI AATATT 1 cut(s) 769
SspMI CTAG 3 cut(s) 63, 392, 913
SstI GAGCTC 1 cut(s) 134
StyD4I CCNGG 1 cut(s) 30
TaaI ACNGT 3 cut(s) 12, 27, 404
TaqI TCGA 3 cut(s) 57, 247, 727
TasI AATT 5 cut(s) 473, 661, 757, 802, 853
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 5 cut(s) 168, 458, 524, 860, 899
Tru1I TTAA 2 cut(s) 357, 885
Tru9I TTAA 2 cut(s) 357, 885
TscAI CASTG 2 cut(s) 32, 409
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 1 cut(s) 20
Tsp45I GTSAC 1 cut(s) 425
TspDTI ATGAA 3 cut(s) 356, 384, 815
TspGWI ACGGA 1 cut(s) 546
TspRI CASTG 2 cut(s) 32, 409
XapI RAATTY 3 cut(s) 661, 757, 853
XmnI GAANNNNTTC 2 cut(s) 602, 761
XspI CTAG 3 cut(s) 63, 392, 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.