Rorug02G0083000

salt tolerance-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004172
Physical Location & Seq
Forward (+)
6690984 .. 6692690
1707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0083000.1

Sequence Viewer

Length: 1419 bp
ATGGAAGACGTAGCTCAAGCAGTTGCAGTTCCATATAGGCTAGGTAATTTGATTCATGAGGAATCAGCAGTGAAAACCCAAATGGAAATCACTGGTCTCAAGCTAATATCAAATACATCAACCTTGTTATCCAATACTTCTTCAAAACCAAGTCTGCCATGTGAGTCCATTTCTTGTGGAAATCAAAGTTACAGAAGCAAAAATCCACAGGACGAAAATAGAGATCTGATTTCTGGTGAAACCATTAATTGGGGAGGTAAGGAGTGCAAGTTCATGCCTTTATGCAATCGGGTTCTAGGTAGTTCATGCTTCCAATCTTTGGTTCATGATGAGGGTGAGATTTGCAATGGATCTAACACTTGTGATGCATCCACCACTTCGGTGAATCGGGCAAAGAAAATTTGTAGGACAAGCTCCAATGGGAAGGTTTTCGATTTGGATCGTGCACCCTTTTGGGGATACACCTCAATCCGTGGAGGGAGACCAGAGATGGAAGATGATGTTGCAGTTATTCCTAGACTTTTGCAGATTCCTATTCAAATGTTAGTGGATGATTCTGACATGAGCCAAAACCCAAGTCAATTAACTGCCCATTTCTTTGGAGTTTATGATGGACATGGGGGTTGTCAGGTTGCTAATTACTGTCGTGAACGAGTGCATTCAGCTTTGGTTGAAGAGATTGAAATTGCTAAAGCAGGCTTGAATGAAAACACTGGTGAGTCTTGGCAGGAGCAGTGGAAGGAGGCCTTCACCAATTGCTTCATAAAAGTTGATGCTGAGGTTGGAGGAGCTCCCAAAGGCACACATTCAAGCAACACTAGTGCTTCTGAGGATTCTCTTAAACCTATTGCTCCTGAAACTGTTGGTTCTACTGCTGTAGTCGTCGTTGTTTGTCCAACCCATATTATAGTAGCAAACTGTGGTGATTCGAGAGCAGTATTGTACCGCGGAAGAGTGGCGATGCCATTGTCAGTGGATCACAAACCAGATAGAGAAGATGAATATGAAAGGATTGAGGCTGCTGGAGGCAAGGTCATACAGTGGGATGGTTCTCGTGTATTTGGTGTTCTTGCCATGTCACGGTCCATAGGTGACAGATACTTGAAACCATCAATCATACCGGATCCAGAAATCATGTTTGTTTCTCGAGAGAAGGAAGACGATTGCCTAATCCTAGCCAGTGACGGCTTGTGGGATGTAATAACAAATGAGGAGGCCTGTGACATTGCGCGGAGGCGGATCCTTGTATGGCATAAGAAGTATGGTGATACAGTAGAACGGGGCGAGGGAGCTGATCCTGCAGCCGAAGCTGCAGCAGTGTACCTCTCAAGGCTTGCAATCCAAAAGGGGAGCAAAGACAACATAACTGTTGTAGTGGTAGACCTGAAAGCTTCAAGGAAGTTTAAGAAAAAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-B_box

Protein Analysis

472

Amino Acids

51.72

Weight (kDa)

5.4

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 185 - 455 3e-60 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 249, 319
AccI GTMKAC 1 cut(s) 1380
AccII CGCG 2 cut(s) 948, 1231
AciI CCGC 4 cut(s) 946, 948, 1231, 1237
AclWI GGATC 8 cut(s) 358, 447, 984, 1118, 1131, 1234, 1247, 1289
AcsI RAATTY 2 cut(s) 399, 1413
AfaI GTAC 2 cut(s) 944, 1322
AfiI CCNNNNNNNGG 4 cut(s) 249, 319, 455, 1080
AgsI TTSAA 8 cut(s) 144, 539, 674, 683, 703, 810, 1105, 1395
AhlI ACTAGT 1 cut(s) 818
AjuI GAANNNNNNNTTGG 2 cut(s) 306, 338
AleI CACNNNNGTG 1 cut(s) 380
AloI GAACNNNNNNTCC 2 cut(s) 254, 286
AluBI AGCT 8 cut(s) 14, 103, 414, 665, 791, 1292, 1310, 1391
AluI AGCT 8 cut(s) 14, 103, 414, 665, 791, 1292, 1310, 1391
Alw21I GWGCWC 2 cut(s) 448, 793
Alw26I GTCTC 2 cut(s) 101, 475
Alw44I GTGCAC 1 cut(s) 444
AlwI GGATC 8 cut(s) 358, 447, 984, 1118, 1131, 1234, 1247, 1289
Ama87I CYCGRG 1 cut(s) 1146
AoxI GGCC 2 cut(s) 744, 1215
ApaLI GTGCAC 1 cut(s) 444
ApeKI GCWGC 4 cut(s) 1019, 1301, 1310, 1313
ApoI RAATTY 2 cut(s) 399, 1413
ArsI GACNNNNNNTTYG 2 cut(s) 562, 594
AseI ATTAAT 1 cut(s) 246
Asp700I GAANNNNTTC 1 cut(s) 428
AspLEI GCGC 1 cut(s) 1231
AspS9I GGNCC 1 cut(s) 1083
AsuHPI GGTGA 8 cut(s) 248, 347, 394, 728, 742, 935, 1103, 1277
AvaI CYCGRG 1 cut(s) 1146
AvaII GGWCC 1 cut(s) 1083
BaeGI GKGCMC 1 cut(s) 448
BamHI GGATCC 2 cut(s) 1123, 1239
BanII GRGCYC 1 cut(s) 793
BauI CACGAG 1 cut(s) 1053
BbsI GAAGAC 2 cut(s) 12, 1164
Bbv12I GWGCWC 2 cut(s) 448, 793
BbvCI CCTCAGC 1 cut(s) 777
BbvI GCAGC 4 cut(s) 1006, 1297, 1313, 1325
BccI CCATC 4 cut(s) 484, 605, 1040, 1117
BceAI ACGGC 1 cut(s) 1201
BciVI GTATCC 1 cut(s) 452
BcoDI GTCTC 2 cut(s) 101, 475
BcuI ACTAGT 1 cut(s) 818
BfaI CTAG 5 cut(s) 41, 296, 516, 819, 1175
BfmI CTRYAG 3 cut(s) 876, 1299, 1311
BfuI GTATCC 1 cut(s) 452
BglII AGATCT 1 cut(s) 223
BisI GCNGC 4 cut(s) 1020, 1302, 1311, 1314
BlsI GCNGC 4 cut(s) 1021, 1303, 1312, 1315
Bme18I GGWCC 1 cut(s) 1083
BmeT110I CYCGRG 1 cut(s) 1146
BmgT120I GGNCC 1 cut(s) 1083
BmiI GGNNCC 2 cut(s) 1125, 1241
BmsI GCATC 4 cut(s) 355, 377, 763, 951
BpiI GAAGAC 2 cut(s) 12, 1164
BpmI CTGGAG 1 cut(s) 1044
Bpu10I CCTNAGC 1 cut(s) 777
BpuEI CTTGAG 2 cut(s) 83, 1312
BsaBI GATNNNNATC 1 cut(s) 438
BsaI GGTCTC 2 cut(s) 101, 475
BsaJI CCNNGG 2 cut(s) 472, 946
BsaWI WCCGGW 1 cut(s) 1120
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 1017, 1047
Bsc4I CCNNNNNNNGG 4 cut(s) 249, 319, 455, 1080
Bse1I ACTGG 3 cut(s) 97, 718, 1179
Bse3DI GCAATG 2 cut(s) 352, 1224
Bse8I GATNNNNATC 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 472, 946
BseGI GGATG 4 cut(s) 368, 556, 1051, 1201
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 4 cut(s) 249, 319, 455, 1080
BseMI GCAATG 2 cut(s) 352, 1224
BseMII CTCAG 2 cut(s) 768, 819
BseNI ACTGG 3 cut(s) 97, 718, 1179
BseRI GAGGAG 2 cut(s) 801, 1226
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 4 cut(s) 1006, 1297, 1313, 1325
Bsh1236I CGCG 2 cut(s) 948, 1231
BshFI GGCC 2 cut(s) 746, 1217
BsiHKAI GWGCWC 2 cut(s) 448, 793
BsiHKCI CYCGRG 1 cut(s) 1146
BsiSI CCGG 1 cut(s) 1121
BslI CCNNNNNNNGG 4 cut(s) 249, 319, 455, 1080
BsmAI GTCTC 2 cut(s) 101, 475
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 2 cut(s) 746, 1217
Bso31I GGTCTC 2 cut(s) 101, 475
BsoBI CYCGRG 1 cut(s) 1146
Bsp1286I GDGCHC 2 cut(s) 448, 793
Bsp143I GATC 7 cut(s) 223, 350, 439, 976, 1123, 1239, 1294
BspACI CCGC 4 cut(s) 946, 948, 1231, 1237
BspANI GGCC 2 cut(s) 746, 1217
BspCNI CTCAG 2 cut(s) 769, 820
BspFNI CGCG 2 cut(s) 948, 1231
BspHI TCATGA 2 cut(s) 55, 325
BspLI GGNNCC 2 cut(s) 1125, 1241
BspMAI CTGCAG 2 cut(s) 1303, 1315
BspPI GGATC 8 cut(s) 358, 447, 984, 1118, 1131, 1234, 1247, 1289
BspTNI GGTCTC 2 cut(s) 101, 475
BsrDI GCAATG 2 cut(s) 352, 1224
BsrI ACTGG 3 cut(s) 97, 718, 1179
BssECI CCNNGG 2 cut(s) 472, 946
BssMI GATC 7 cut(s) 223, 350, 439, 976, 1123, 1239, 1294
BssSI CACGAG 1 cut(s) 1053
Bst2BI CACGAG 1 cut(s) 1053
Bst4CI ACNGT 7 cut(s) 644, 862, 920, 1041, 1083, 1273, 1369
Bst6I CTCTTC 2 cut(s) 669, 946
BstC8I GCNNGC 2 cut(s) 697, 1335
BstDEI CTNAG 2 cut(s) 777, 828
BstDSI CCRYGG 2 cut(s) 472, 946
BstF5I GGATG 4 cut(s) 368, 556, 1051, 1201
BstFNI CGCG 2 cut(s) 948, 1231
BstHHI GCGC 1 cut(s) 1231
BstKTI GATC 7 cut(s) 226, 353, 442, 979, 1126, 1242, 1297
BstMAI GTCTC 2 cut(s) 101, 475
BstMBI GATC 7 cut(s) 223, 350, 439, 976, 1123, 1239, 1294
BstMWI GCNNNNNNNGC 3 cut(s) 1298, 1307, 1310
BstSFI CTRYAG 3 cut(s) 876, 1299, 1311
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 2 cut(s) 948, 1231
BstV1I GCAGC 4 cut(s) 1006, 1297, 1313, 1325
BstV2I GAAGAC 2 cut(s) 12, 1164
BstX2I RGATCY 4 cut(s) 223, 350, 1123, 1239
BstXI CCANNNNNNTGG 1 cut(s) 599
BstYI RGATCY 4 cut(s) 223, 350, 1123, 1239
BsuI GTATCC 1 cut(s) 452
BsuRI GGCC 2 cut(s) 746, 1217
BtgI CCRYGG 2 cut(s) 472, 946
BtgZI GCGATG 1 cut(s) 974
BtsCI GGATG 4 cut(s) 368, 556, 1051, 1201
BtsI GCAGTG 3 cut(s) 75, 740, 1323
BtsIMutI CAGTG 8 cut(s) 75, 90, 711, 740, 978, 1046, 1186, 1323
Cac8I GCNNGC 2 cut(s) 697, 1335
CciI TCATGA 2 cut(s) 55, 325
CfoI GCGC 1 cut(s) 1231
Cfr13I GGNCC 1 cut(s) 1083
Cfr42I CCGCGG 1 cut(s) 949
Csp6I GTAC 2 cut(s) 943, 1321
CviAII CATG 9 cut(s) 56, 159, 274, 306, 326, 562, 617, 1075, 1135
CviQI GTAC 2 cut(s) 943, 1321
DdeI CTNAG 2 cut(s) 777, 828
DpnI GATC 7 cut(s) 225, 352, 441, 978, 1125, 1241, 1296
DpnII GATC 7 cut(s) 223, 350, 439, 976, 1123, 1239, 1294
Eam1104I CTCTTC 2 cut(s) 669, 946
EarI CTCTTC 2 cut(s) 669, 946
EciI GGCGGA 1 cut(s) 1252
Ecl136II GAGCTC 1 cut(s) 791
Eco147I AGGCCT 2 cut(s) 746, 1217
Eco24I GRGCYC 1 cut(s) 793
Eco31I GGTCTC 2 cut(s) 101, 475
Eco47I GGWCC 1 cut(s) 1083
Eco53kI GAGCTC 1 cut(s) 791
Eco88I CYCGRG 1 cut(s) 1146
EcoICRI GAGCTC 1 cut(s) 791
EcoT22I ATGCAT 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 793
FaeI CATG 9 cut(s) 59, 162, 277, 309, 329, 565, 620, 1078, 1138
FalI AAGNNNNNCTT 2 cut(s) 731, 763
FatI CATG 9 cut(s) 55, 158, 273, 305, 325, 561, 616, 1074, 1134
FblI GTMKAC 1 cut(s) 1380
Fnu4HI GCNGC 4 cut(s) 1020, 1302, 1311, 1314
FokI GGATG 4 cut(s) 355, 563, 1058, 1208
FriOI GRGCYC 1 cut(s) 793
Fsp4HI GCNGC 4 cut(s) 1020, 1302, 1311, 1314
FspBI CTAG 5 cut(s) 41, 296, 516, 819, 1175
GlaI GCGC 1 cut(s) 1230
GluI GCNGC 4 cut(s) 1020, 1302, 1311, 1314
GsuI CTGGAG 1 cut(s) 1044
HaeIII GGCC 2 cut(s) 746, 1217
HapII CCGG 1 cut(s) 1121
HhaI GCGC 1 cut(s) 1231
Hin1II CATG 9 cut(s) 59, 162, 277, 309, 329, 565, 620, 1078, 1138
Hin6I GCGC 1 cut(s) 1229
HinP1I GCGC 1 cut(s) 1229
HindIII AAGCTT 1 cut(s) 1389
HinfI GANTC 9 cut(s) 52, 62, 164, 385, 529, 554, 719, 833, 926
HpaII CCGG 1 cut(s) 1121
HphI GGTGA 8 cut(s) 248, 347, 394, 728, 742, 935, 1103, 1277
Hpy166II GTNNAC 4 cut(s) 446, 650, 1321, 1381
Hpy188I TCNGA 3 cut(s) 228, 559, 829
Hpy188III TCNNGA 8 cut(s) 56, 326, 647, 854, 930, 1127, 1146, 1148
Hpy8I GTNNAC 4 cut(s) 446, 650, 1321, 1381
Hpy99I CGWCG 1 cut(s) 887
HpyAV CCTTC 4 cut(s) 418, 733, 757, 1147
HpyCH4III ACNGT 7 cut(s) 644, 862, 920, 1041, 1083, 1273, 1369
HpyCH4IV ACGT 1 cut(s) 9
HpyF10VI GCNNNNNNNGC 3 cut(s) 1298, 1307, 1310
HpyF3I CTNAG 2 cut(s) 777, 828
HpySE526I ACGT 1 cut(s) 9
Hsp92II CATG 9 cut(s) 59, 162, 277, 309, 329, 565, 620, 1078, 1138
HspAI GCGC 1 cut(s) 1229
KspI CCGCGG 1 cut(s) 949
Kzo9I GATC 7 cut(s) 223, 350, 439, 976, 1123, 1239, 1294
LmnI GCTCC 7 cut(s) 419, 730, 788, 796, 856, 1289, 1350
Lsp1109I GCAGC 4 cut(s) 1006, 1297, 1313, 1325
LweI GCATC 4 cut(s) 355, 377, 763, 951
MaeI CTAG 5 cut(s) 41, 296, 516, 819, 1175
MaeII ACGT 1 cut(s) 9
MaeIII GTNAC 5 cut(s) 188, 1077, 1091, 1181, 1220
MalI GATC 7 cut(s) 225, 352, 441, 978, 1125, 1241, 1296
MboI GATC 7 cut(s) 223, 350, 439, 976, 1123, 1239, 1294
MboII GAAGA 7 cut(s) 17, 132, 506, 686, 963, 1007, 1169
MfeI CAATTG 1 cut(s) 754
MflI RGATCY 4 cut(s) 223, 350, 1123, 1239
MhlI GDGCHC 2 cut(s) 448, 793
MluCI AATT 8 cut(s) 46, 247, 399, 581, 637, 684, 754, 1413
MlyI GAGTC 2 cut(s) 173, 728
MmeI TCCRAC 2 cut(s) 763, 920
Mph1103I ATGCAT 1 cut(s) 370
MroXI GAANNNNTTC 1 cut(s) 428
MseI TTAA 5 cut(s) 246, 584, 840, 1404, 1417
MslI CAYNNNNRTG 1 cut(s) 380
MspA1I CMGCKG 1 cut(s) 948
MspI CCGG 1 cut(s) 1121
MunI CAATTG 1 cut(s) 754
Mva1269I GAATGC 1 cut(s) 658
MvnI CGCG 2 cut(s) 948, 1231
MwoI GCNNNNNNNGC 3 cut(s) 1298, 1307, 1310
NdeII GATC 7 cut(s) 223, 350, 439, 976, 1123, 1239, 1294
NlaIII CATG 9 cut(s) 59, 162, 277, 309, 329, 565, 620, 1078, 1138
NlaIV GGNNCC 2 cut(s) 1125, 1241
NmuCI GTSAC 4 cut(s) 1077, 1091, 1181, 1220
NsiI ATGCAT 1 cut(s) 370
OliI CACNNNNGTG 1 cut(s) 380
PaeR7I CTCGAG 1 cut(s) 1146
PagI TCATGA 2 cut(s) 55, 325
PceI AGGCCT 2 cut(s) 746, 1217
PctI GAATGC 1 cut(s) 658
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 7 cut(s) 52, 62, 385, 529, 554, 833, 926
PflMI CCANNNNNTGG 2 cut(s) 249, 319
PkrI GCNGC 4 cut(s) 1021, 1303, 1312, 1315
PleI GAGTC 2 cut(s) 172, 727
PpsI GAGTC 2 cut(s) 172, 727
PshBI ATTAAT 1 cut(s) 246
Psp124BI GAGCTC 1 cut(s) 793
PspN4I GGNNCC 2 cut(s) 1125, 1241
PspPI GGNCC 1 cut(s) 1083
PsrI GAACNNNNNNTAC 2 cut(s) 1050, 1082
PstI CTGCAG 2 cut(s) 1303, 1315
PsuI RGATCY 4 cut(s) 223, 350, 1123, 1239
RsaI GTAC 2 cut(s) 944, 1322
RsaNI GTAC 2 cut(s) 943, 1321
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 1 cut(s) 793
SacII CCGCGG 1 cut(s) 949
SaqAI TTAA 5 cut(s) 246, 584, 840, 1404, 1417
SatI GCNGC 4 cut(s) 1020, 1302, 1311, 1314
Sau3AI GATC 7 cut(s) 223, 350, 439, 976, 1123, 1239, 1294
Sau96I GGNCC 1 cut(s) 1083
SchI GAGTC 2 cut(s) 173, 728
SduI GDGCHC 2 cut(s) 448, 793
SfaNI GCATC 4 cut(s) 355, 377, 763, 951
SfcI CTRYAG 3 cut(s) 876, 1299, 1311
Sfr274I CTCGAG 1 cut(s) 1146
Sfr303I CCGCGG 1 cut(s) 949
SgrBI CCGCGG 1 cut(s) 949
SinI GGWCC 1 cut(s) 1083
SlaI CTCGAG 1 cut(s) 1146
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 4 cut(s) 15, 98, 1146, 1327
SmoI CTYRAG 4 cut(s) 15, 98, 1146, 1327
SpeI ACTAGT 1 cut(s) 818
Sse9I AATT 8 cut(s) 46, 247, 399, 581, 637, 684, 754, 1413
SseBI AGGCCT 2 cut(s) 746, 1217
SsiI CCGC 4 cut(s) 946, 948, 1231, 1237
SspMI CTAG 5 cut(s) 41, 296, 516, 819, 1175
SstI GAGCTC 1 cut(s) 793
StuI AGGCCT 2 cut(s) 746, 1217
TaaI ACNGT 7 cut(s) 644, 862, 920, 1041, 1083, 1273, 1369
TaiI ACGT 1 cut(s) 12
TaqI TCGA 3 cut(s) 432, 929, 1147
TasI AATT 8 cut(s) 46, 247, 399, 581, 637, 684, 754, 1413
TfiI GAWTC 7 cut(s) 52, 62, 385, 529, 554, 833, 926
Tru1I TTAA 5 cut(s) 246, 584, 840, 1404, 1417
Tru9I TTAA 5 cut(s) 246, 584, 840, 1404, 1417
TscAI CASTG 8 cut(s) 75, 97, 718, 740, 978, 1046, 1186, 1323
TseFI GTSAC 4 cut(s) 1077, 1091, 1181, 1220
TseI GCWGC 4 cut(s) 1019, 1301, 1310, 1313
Tsp45I GTSAC 4 cut(s) 1077, 1091, 1181, 1220
TspDTI ATGAA 8 cut(s) 44, 262, 294, 314, 720, 751, 1014, 1020
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 8 cut(s) 75, 97, 718, 740, 978, 1046, 1186, 1323
Van91I CCANNNNNTGG 2 cut(s) 249, 319
VneI GTGCAC 1 cut(s) 444
VpaK11BI GGWCC 1 cut(s) 1083
VspI ATTAAT 1 cut(s) 246
XapI RAATTY 2 cut(s) 399, 1413
XhoI CTCGAG 1 cut(s) 1146
XmiI GTMKAC 1 cut(s) 1380
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 5 cut(s) 41, 296, 516, 819, 1175
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.