Rorug02G0115100

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007545
Physical Location & Seq
Reverse (-)
9806301 .. 9808711
2411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0115100.1

Sequence Viewer

Length: 1206 bp
ATGGATGACACCGTCGACTGGTCCAATCTTCCGGTCGAACTCTGGCCACTGATCGGAGAAAAGCTCAAAGTCCGCATCGATTCTCTCCGATTCCGCAGCGTTTGCCATTCATGGCGTTCTTGTTTGCCTCGCTTCCACCCTTCACCTCTTCCGAAATTCCCTCTCCCACTCTCCGATGGACCCACCTCCTTCCTCTCCCAAAACACCGTCTACCTCCTCCAAACCCCCTCCACGTCATCACTGGCGCCGCCGTCGTGTGGAGGTTGGCTGGTCAAGGTCGAAGAGTGCGACAGAATGATTCTTCGGAATCCGATCACCTCCCGGCGAATCAGGTACTCAAACGACTCGTCGTCTCCGGAATGTTTCAATTTGCTGGAGTTCAGAATGATCGAATTAGGGAAGTCGTATTTTCTGAGATACATCCAGGGCTCTGGTTCTGTTGTTGGTTTGAAGAAAGTGATTTTACTGCATCCTGAGATTGTTGATTGTGCTATTCTGGTTATGTCTGATAAAGGAAATTTGGGTTTTGCTAGAAGTGGAGATGAGAAACTAACACGGTTTAGTAATGGAGGTTTTGTTTTTGATGATGTTATTGTTTTTAAGGGTCAGCCATATGTGGTGGATAGCTTGGGTTGTGTTTTTAGGATTGACGTGTCCTCAGAAGCGATGGTGAAGATTTCGTCGAAAGTGGTTGGTTTTGGTGGGAGGAAGCATTTGGTGGAGTCTTGTGGAGAGCTTTATGTGGTCGATAGGTATTTTGATCAAGAACAAAATAAGCAACAAGGGACTGGTGTTGAGTCAGATTTTTCTCGTTTTGTCCTTTGGCGTGGTGGAAGACACCAAAGATATCAGTCTGGTGAACCCAAGGTAGTTGATTTTATGGTTTACAAGTTGGAACTGTTGGATGGAGAGTTGGGCAGATGGGTTGAAGTGAAGAGCTTGGGTGATAGAGCGTTTTTCTTGGCTGTGGATTGCTGTTTCTCTGTTTTGGCTCAAGATTTAGCTGGGTACAAAGGGAATTGCATTTACTTTTCAAATGAAAACAATGTTAATCTTGCTCTTAGAGAAGTAACTAGACCAGAGAGTTTTGTGTTCAACTTAGAGGACCATAGCATTCAGAAGTTGGGATCTTCCCCAGGTGGTTCCCAGATATTTTGGCCACCTCCAATTGAGCTCGGCTCCACATGTTCCTATTCTCTGGATTGA

Protein Analysis

401

Amino Acids

45.1

Weight (kDa)

6.14

Isoelectric Point (pI)

47.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 66 - 353 2.6e-41 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 244
AccI GTMKAC 2 cut(s) 15, 210
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 3 cut(s) 73, 94, 248
AclWI GGATC 1 cut(s) 1135
AcoI YGGCCR 2 cut(s) 44, 1157
AcsI RAATTY 2 cut(s) 155, 517
AcyI GRCGYC 1 cut(s) 245
AfaI GTAC 2 cut(s) 335, 1010
AfiI CCNNNNNNNGG 3 cut(s) 18, 53, 257
AflIII ACRYGT 2 cut(s) 651, 1184
AgsI TTSAA 5 cut(s) 367, 451, 929, 1035, 1096
AhdI GACNNNNNGTC 1 cut(s) 349
AjiI CACGTC 2 cut(s) 234, 652
AjnI CCWGG 2 cut(s) 423, 1135
AluBI AGCT 6 cut(s) 64, 627, 736, 939, 1004, 1174
AluI AGCT 6 cut(s) 64, 627, 736, 939, 1004, 1174
Alw21I GWGCWC 1 cut(s) 1176
Alw26I GTCTC 1 cut(s) 357
AlwI GGATC 1 cut(s) 1135
Aor13HI TCCGGA 1 cut(s) 355
AoxI GGCC 2 cut(s) 44, 1157
ApeKI GCWGC 1 cut(s) 96
ApoI RAATTY 2 cut(s) 155, 517
ArsI GACNNNNNNTTYG 2 cut(s) 332, 364
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 3 cut(s) 21, 179, 1105
AsuC2I CCSGG 1 cut(s) 322
AsuHPI GGTGA 5 cut(s) 135, 307, 682, 869, 956
AvaII GGWCC 3 cut(s) 21, 179, 1105
BalI TGGCCA 2 cut(s) 46, 1159
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 2 cut(s) 431, 1176
BbsI GAAGAC 1 cut(s) 841
Bbv12I GWGCWC 1 cut(s) 1176
BbvI GCAGC 1 cut(s) 108
BccI CCATC 4 cut(s) 170, 661, 899, 915
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 2 cut(s) 425, 1137
BclI TGATCA 1 cut(s) 760
BcnI CCSGG 1 cut(s) 322
BcoDI GTCTC 1 cut(s) 357
BfaI CTAG 2 cut(s) 531, 1074
BfoI RGCGCY 1 cut(s) 248
BisI GCNGC 2 cut(s) 97, 248
BlsI GCNGC 2 cut(s) 98, 249
Bme1390I CCNGG 3 cut(s) 322, 425, 1137
Bme18I GGWCC 3 cut(s) 21, 179, 1105
BmeRI GACNNNNNGTC 1 cut(s) 349
BmgBI CACGTC 2 cut(s) 234, 652
BmgT120I GGNCC 3 cut(s) 21, 179, 1105
BmiI GGNNCC 4 cut(s) 181, 246, 1144, 1180
BmrFI CCNGG 3 cut(s) 322, 425, 1137
BmsI GCATC 2 cut(s) 84, 478
BpiI GAAGAC 1 cut(s) 841
BplI GAGNNNNNCTC 4 cut(s) 48, 80, 1163, 1195
BpmI CTGGAG 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 978
BpuMI CCSGG 1 cut(s) 322
Bsa29I ATCGAT 1 cut(s) 78
BsaHI GRCGYC 1 cut(s) 245
BsaJI CCNNGG 3 cut(s) 424, 864, 1135
BsaWI WCCGGW 2 cut(s) 31, 355
BsaXI ACNNNNNCTCC 2 cut(s) 561, 591
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 53, 257
Bse1I ACTGG 3 cut(s) 23, 246, 793
BseAI TCCGGA 1 cut(s) 355
BseBI CCWGG 2 cut(s) 425, 1137
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 3 cut(s) 424, 864, 1135
BseGI GGATG 4 cut(s) 10, 420, 469, 910
BseLI CCNNNNNNNGG 3 cut(s) 18, 53, 257
BseMII CTCAG 3 cut(s) 404, 465, 672
BseNI ACTGG 3 cut(s) 23, 246, 793
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 108
BseYI CCCAGC 1 cut(s) 1004
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 46, 1159
BshNI GGYRCC 1 cut(s) 244
BshVI ATCGAT 1 cut(s) 78
BsiEI CGRYCG 1 cut(s) 36
BsiHKAI GWGCWC 1 cut(s) 1176
BsiSI CCGG 3 cut(s) 32, 322, 356
BslFI GGGAC 1 cut(s) 799
BslI CCNNNNNNNGG 3 cut(s) 18, 53, 257
BsmAI GTCTC 1 cut(s) 357
BsmBI CGTCTC 1 cut(s) 357
BsmFI GGGAC 1 cut(s) 799
BsmI GAATGC 1 cut(s) 1113
BsnI GGCC 2 cut(s) 46, 1159
Bsp1286I GDGCHC 2 cut(s) 431, 1176
Bsp13I TCCGGA 1 cut(s) 355
Bsp143I GATC 5 cut(s) 51, 312, 387, 760, 1127
BspACI CCGC 3 cut(s) 73, 94, 248
BspANI GGCC 2 cut(s) 46, 1159
BspCNI CTCAG 3 cut(s) 405, 466, 671
BspDI ATCGAT 1 cut(s) 78
BspEI TCCGGA 1 cut(s) 355
BspLI GGNNCC 4 cut(s) 181, 246, 1144, 1180
BspPI GGATC 1 cut(s) 1135
BspQI GCTCTTC 1 cut(s) 929
BspT107I GGYRCC 1 cut(s) 244
BsrI ACTGG 3 cut(s) 23, 246, 793
BssECI CCNNGG 3 cut(s) 424, 864, 1135
BssMI GATC 5 cut(s) 51, 312, 387, 760, 1127
BssNI GRCGYC 1 cut(s) 245
BssT1I CCWWGG 1 cut(s) 864
Bst2UI CCWGG 2 cut(s) 425, 1137
Bst4CI ACNGT 4 cut(s) 13, 208, 558, 900
Bst6I CTCTTC 3 cut(s) 153, 276, 929
BstACI GRCGYC 1 cut(s) 245
BstAPI GCANNNNNTGC 1 cut(s) 102
BstDEI CTNAG 5 cut(s) 413, 474, 658, 1061, 1099
BstF5I GGATG 4 cut(s) 10, 420, 469, 910
BstH2I RGCGCY 1 cut(s) 248
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 5 cut(s) 54, 315, 390, 763, 1130
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 5 cut(s) 51, 312, 387, 760, 1127
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 1 cut(s) 102
BstNI CCWGG 2 cut(s) 425, 1137
BstNSI RCATGY 1 cut(s) 1188
BstSCI CCNGG 3 cut(s) 320, 423, 1135
BstV1I GCAGC 1 cut(s) 108
BstV2I GAAGAC 1 cut(s) 841
BstX2I RGATCY 1 cut(s) 1127
BstXI CCANNNNNNTGG 1 cut(s) 431
BstYI RGATCY 1 cut(s) 1127
Bsu15I ATCGAT 1 cut(s) 78
BsuRI GGCC 2 cut(s) 46, 1159
BsuTUI ATCGAT 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 680
BtrI CACGTC 2 cut(s) 234, 652
BtsCI GGATG 4 cut(s) 10, 420, 469, 910
BtsIMutI CAGTG 2 cut(s) 47, 239
CfoI GCGC 1 cut(s) 247
Cfr13I GGNCC 3 cut(s) 21, 179, 1105
ClaI ATCGAT 1 cut(s) 78
Csp6I GTAC 2 cut(s) 334, 1009
CviAII CATG 2 cut(s) 111, 1185
CviQI GTAC 2 cut(s) 334, 1009
DdeI CTNAG 5 cut(s) 413, 474, 658, 1061, 1099
DinI GGCGCC 1 cut(s) 246
DpnI GATC 5 cut(s) 53, 314, 389, 762, 1129
DpnII GATC 5 cut(s) 51, 312, 387, 760, 1127
DriI GACNNNNNGTC 1 cut(s) 349
EaeI YGGCCR 2 cut(s) 44, 1157
Eam1104I CTCTTC 3 cut(s) 153, 276, 929
Eam1105I GACNNNNNGTC 1 cut(s) 349
EarI CTCTTC 3 cut(s) 153, 276, 929
Ecl136II GAGCTC 1 cut(s) 1174
Eco130I CCWWGG 1 cut(s) 864
Eco24I GRGCYC 2 cut(s) 431, 1176
Eco32I GATATC 1 cut(s) 848
Eco47I GGWCC 3 cut(s) 21, 179, 1105
Eco53kI GAGCTC 1 cut(s) 1174
EcoICRI GAGCTC 1 cut(s) 1174
EcoRII CCWGG 2 cut(s) 423, 1135
EcoRV GATATC 1 cut(s) 848
EcoT14I CCWWGG 1 cut(s) 864
EcoT38I GRGCYC 2 cut(s) 431, 1176
EgeI GGCGCC 1 cut(s) 246
EheI GGCGCC 1 cut(s) 246
ErhI CCWWGG 1 cut(s) 864
Esp3I CGTCTC 1 cut(s) 357
FaeI CATG 2 cut(s) 114, 1188
FaiI YATR 8 cut(s) 112, 503, 613, 615, 741, 881, 1110, 1186
FaqI GGGAC 1 cut(s) 799
FatI CATG 2 cut(s) 110, 1184
FauNDI CATATG 1 cut(s) 613
FbaI TGATCA 1 cut(s) 760
FblI GTMKAC 2 cut(s) 15, 210
Fnu4HI GCNGC 2 cut(s) 97, 248
FokI GGATG 4 cut(s) 17, 407, 456, 917
FriOI GRGCYC 2 cut(s) 431, 1176
Fsp4HI GCNGC 2 cut(s) 97, 248
FspBI CTAG 2 cut(s) 531, 1074
GlaI GCGC 1 cut(s) 246
GluI GCNGC 2 cut(s) 97, 248
GsaI CCCAGC 1 cut(s) 1008
GsuI CTGGAG 1 cut(s) 395
HaeII RGCGCY 1 cut(s) 248
HaeIII GGCC 2 cut(s) 46, 1159
HapII CCGG 3 cut(s) 32, 322, 356
HhaI GCGC 1 cut(s) 247
Hin1I GRCGYC 1 cut(s) 245
Hin1II CATG 2 cut(s) 114, 1188
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 8 cut(s) 80, 90, 298, 307, 327, 344, 722, 797
HpaII CCGG 3 cut(s) 32, 322, 356
HphI GGTGA 5 cut(s) 135, 307, 682, 869, 956
Hpy166II GTNNAC 4 cut(s) 16, 211, 860, 886
Hpy188III TCNNGA 5 cut(s) 356, 473, 764, 995, 1199
Hpy8I GTNNAC 4 cut(s) 16, 211, 860, 886
Hpy99I CGWCG 4 cut(s) 17, 256, 352, 685
HpyAV CCTTC 2 cut(s) 150, 199
HpyCH4III ACNGT 4 cut(s) 13, 208, 558, 900
HpyCH4IV ACGT 2 cut(s) 233, 651
HpyCH4V TGCA 2 cut(s) 469, 1023
HpyF10VI GCNNNNNNNGC 1 cut(s) 102
HpyF3I CTNAG 5 cut(s) 413, 474, 658, 1061, 1099
HpySE526I ACGT 2 cut(s) 233, 651
Hsp92I GRCGYC 1 cut(s) 245
Hsp92II CATG 2 cut(s) 114, 1188
HspAI GCGC 1 cut(s) 245
KasI GGCGCC 1 cut(s) 244
Kpn2I TCCGGA 1 cut(s) 355
Ksp22I TGATCA 1 cut(s) 760
Kzo9I GATC 5 cut(s) 51, 312, 387, 760, 1127
LguI GCTCTTC 1 cut(s) 929
LmnI GCTCC 1 cut(s) 1184
Lsp1109I GCAGC 1 cut(s) 108
LweI GCATC 2 cut(s) 84, 478
MaeI CTAG 2 cut(s) 531, 1074
MaeII ACGT 2 cut(s) 233, 651
MaeIII GTNAC 1 cut(s) 1069
MalI GATC 5 cut(s) 53, 314, 389, 762, 1129
MboI GATC 5 cut(s) 51, 312, 387, 760, 1127
MboII GAAGA 9 cut(s) 20, 140, 293, 293, 463, 685, 846, 946, 1122
MfeI CAATTG 1 cut(s) 1167
MflI RGATCY 1 cut(s) 1127
MhlI GDGCHC 2 cut(s) 431, 1176
MlsI TGGCCA 2 cut(s) 46, 1159
MluCI AATT 6 cut(s) 155, 367, 392, 517, 1018, 1167
MluNI TGGCCA 2 cut(s) 46, 1159
Mly113I GGCGCC 1 cut(s) 245
MlyI GAGTC 3 cut(s) 338, 731, 806
MmeI TCCRAC 2 cut(s) 873, 882
Mox20I TGGCCA 2 cut(s) 46, 1159
MroI TCCGGA 1 cut(s) 355
MscI TGGCCA 2 cut(s) 46, 1159
MseI TTAA 2 cut(s) 600, 1050
Msp20I TGGCCA 2 cut(s) 46, 1159
MspI CCGG 3 cut(s) 32, 322, 356
MspR9I CCNGG 3 cut(s) 322, 425, 1137
MunI CAATTG 1 cut(s) 1167
Mva1269I GAATGC 1 cut(s) 1113
MvaI CCWGG 2 cut(s) 425, 1137
MwoI GCNNNNNNNGC 1 cut(s) 102
NarI GGCGCC 1 cut(s) 245
NciI CCSGG 1 cut(s) 322
NdeI CATATG 1 cut(s) 613
NdeII GATC 5 cut(s) 51, 312, 387, 760, 1127
NlaIII CATG 2 cut(s) 114, 1188
NlaIV GGNNCC 4 cut(s) 181, 246, 1144, 1180
NmeAIII GCCGAG 1 cut(s) 1155
NspI RCATGY 1 cut(s) 1188
PciI ACATGT 1 cut(s) 1184
PciSI GCTCTTC 1 cut(s) 929
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 1 cut(s) 1113
PfeI GAWTC 5 cut(s) 80, 90, 298, 307, 327
PflFI GACNNNGTC 1 cut(s) 11
PkrI GCNGC 2 cut(s) 98, 249
PleI GAGTC 3 cut(s) 338, 730, 805
PluTI GGCGCC 1 cut(s) 248
PpsI GAGTC 3 cut(s) 338, 730, 805
PscI ACATGT 1 cut(s) 1184
Psp124BI GAGCTC 1 cut(s) 1176
Psp6I CCWGG 2 cut(s) 423, 1135
PspFI CCCAGC 1 cut(s) 1004
PspGI CCWGG 2 cut(s) 423, 1135
PspN4I GGNNCC 4 cut(s) 181, 246, 1144, 1180
PspPI GGNCC 3 cut(s) 21, 179, 1105
PsuI RGATCY 1 cut(s) 1127
PsyI GACNNNGTC 1 cut(s) 11
RsaI GTAC 2 cut(s) 335, 1010
RsaNI GTAC 2 cut(s) 334, 1009
SacI GAGCTC 1 cut(s) 1176
SalI GTCGAC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 929
SaqAI TTAA 2 cut(s) 600, 1050
SatI GCNGC 2 cut(s) 97, 248
Sau3AI GATC 5 cut(s) 51, 312, 387, 760, 1127
Sau96I GGNCC 3 cut(s) 21, 179, 1105
SchI GAGTC 3 cut(s) 338, 731, 806
ScrFI CCNGG 3 cut(s) 322, 425, 1137
SduI GDGCHC 2 cut(s) 431, 1176
SfaNI GCATC 2 cut(s) 84, 478
SfoI GGCGCC 1 cut(s) 246
SinI GGWCC 3 cut(s) 21, 179, 1105
SmlI CTYRAG 1 cut(s) 993
SmoI CTYRAG 1 cut(s) 993
Sse9I AATT 6 cut(s) 155, 367, 392, 517, 1018, 1167
SsiI CCGC 3 cut(s) 73, 94, 248
SspDI GGCGCC 1 cut(s) 244
SspMI CTAG 2 cut(s) 531, 1074
SstI GAGCTC 1 cut(s) 1176
StyD4I CCNGG 3 cut(s) 320, 423, 1135
StyI CCWWGG 1 cut(s) 864
TaaI ACNGT 4 cut(s) 13, 208, 558, 900
TaiI ACGT 2 cut(s) 236, 654
TaqI TCGA 7 cut(s) 15, 36, 78, 279, 390, 683, 747
TasI AATT 6 cut(s) 155, 367, 392, 517, 1018, 1167
TauI GCSGC 1 cut(s) 250
TfiI GAWTC 5 cut(s) 80, 90, 298, 307, 327
Tru1I TTAA 2 cut(s) 600, 1050
Tru9I TTAA 2 cut(s) 600, 1050
TscAI CASTG 2 cut(s) 54, 246
TseI GCWGC 1 cut(s) 96
TspDTI ATGAA 2 cut(s) 99, 1053
TspRI CASTG 2 cut(s) 54, 246
Tth111I GACNNNGTC 1 cut(s) 11
VpaK11BI GGWCC 3 cut(s) 21, 179, 1105
XapI RAATTY 2 cut(s) 155, 517
XceI RCATGY 1 cut(s) 1188
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XmiI GTMKAC 2 cut(s) 15, 210
XspI CTAG 2 cut(s) 531, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.