Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0001341

Physical Location & Seq

Genomic Coordinates Reverse (-)

30250295 .. 30253364

Gene Structure 3070 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0280400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1644 bp

ATGGACGACATCTCCCGACGAAAGCAGCTGATGAGCTCTCGCCGACGATCATGGTGTTGCTCATTCACAGACCCACCCTCAAGCCCCGAATCCTTCTCCAACCCTAAACTCTCCAAACCCCTCCAAAAGTACGACACTTTCTCCAAACCCAACTCCAACTCCGTCCCCAGCTCCCCCCAGAGCACCAAATCCGGGTTGAGCCTGGTGGGCCGGATCGACCCCCGTCGGATCTTGTCCCCGGGTCGCGTCTCCCCAATCGATTCCAGCCCGATCCAGCAACTGGGCCCCGCCCCATCTCCTCCTCCGCCTCCCACCGTCGATTCCGCCGCCGCCCAGATGAGATCCCAGAGCTTCCGGGCCCCGGCCCAGGTGAGAGCCGAGAGCGTGTCGAGCTCCGAGTGCAATGTGTTCGATGTGAGGTTGAATTTGAGAGGGAAGAGGGGCGGGGGTTTGGTTCTTGAGCTCAGCGCCGACGTTCTGAGTTCGAATTCGGAGCTTTTCGCCGGATTGATCAGTGAGTATAGAAAGAGTAATAATGGGTCGTCGTCGGCGGCGTCGTCTTCGGGGTCGAAAATGTGTCGAATTGAGGTGCCTGAGGTGGAGAATTTGTCAGTTTTTAGAGAAGCCATTGAGCTCATGTTTGAGGATGATGACATCACCAAGCGCCTCTTCAGGATTGGGGTGTATAGGGTTATTGATGTGCTTGAGGTATCTGCAGGCATCATGTTCACTAAGGGTGTCTTGTCCTGTTTAAAATATCTTGAGGCTGTTCCTTGGACTGAGGAAGAAGAGGAGAAACTTAGGAACCTGTTTACAAGGTTTAAGTTTGATGATGCAACTACTAGAGACATCTTGGCCAGACTTTACTTGCTTGACTCTGTCGATTCTCAACAAAACTTATCCAGACAACTTGTTTCATCTATCACCACTTGTACAGATGCAAATGCAAGGCATGAGCTGAAGTTGTTAGTCAAGGGTCTCCTGTGCAGAAGTTCAGTGTATGAGAAGGACCATCATGACCTAAATAAGGAAGATATTTATGGTGTATGTCGATCATGTCTGTCTTCACTAGTTAGTCTTTTTGAGGAGGCCACTGGCAACATTTCTCATCAGAAAACTGTGAAGGAGACAGGTAAGCCTATGATAGAACGTGTCTCAAGGCAGGTTGATAATATCAACTGGCTGCTGGACATCCTGCTTGATCGGCAAATGGCTGAAGATTTTGTGGATATATGGGCTGAGCAGGGAGAACTTCTTAAAATGCATGGCATAACTTCTCCTATGGTCAGGTATGAGCTGAGCCGAGTTTCTGCAATTTTGTTTGTTGCAATGGGGACCCGAAAACTACAATGCCGGTCAGAGTCGAGATCAGGGCTTCTTCAGGCATGGTTTGGTCCAATGCTTTTGGATTTTGGTTGGCTGCAGAGATGTCGGAAGGGGCTTGACATAAAGAGTTTGGAGGAAGCTCTGGGTCAGACACTCCTTACGCTACCATTGAAGCAGCAGTATATGCTTTTTATGGAATGGTTTCAATATTTCTCAAAGCACAGCACCGACTGCCCTAACCTCAGCAAAGCCTTCCAGATATGGTGGCGTAGATCATTTCTAAGAGGCTCCGAAACTTCTGCTATTGAATCCAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006355 GO:0006357 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0008022 GO:0008150 GO:0008152 GO:0008420 GO:0009628 GO:0009651 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0016311 GO:0016591 GO:0016787 GO:0016788 GO:0016791 GO:0019219 GO:0019222 GO:0019538 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032991 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070940 GO:0071704 GO:0080090 GO:0140096 GO:1901564 GO:1902494 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRCT NIF PTCB-BRCT

Protein Analysis

547

Amino Acids

61.69

Weight (kDa)

8.5

Isoelectric Point (pI)

66.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB-POZ_ANK-like	PF25553	294 - 537	1.6e-133	BTB/POZ Ankyrin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001341)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g10330
rosa_chinensis	RchiOBHm_Chr2g0124471 RchiOBHm_Chr2g0128771 RchiOBHm_Chr3g0488911 RchiOBHm_Chr7g0210561 RchiOBHm_Chr7g0211191 RchiOBHm_Chr7g0211371 RchiOBHm_Chr7g0211471 RchiOBHm_Chr7g0211491
rosa_laevigata	RLG00000003001 RLG00000018803 RLG00000022902
rosa_multiflora	Rmu_co8429473.1_g000001 Rmu_sc0001208.1_g000002 Rmu_sc0001303.1_g000009 Rmu_sc0002283.1_g000067 Rmu_sc0005514.1_g000010 Rmu_sc0007204.1_g000013 Rmu_sc0018619.1_g000009
rosa_roxburghii	Rroxscaffold_1G00018290 Rroxscaffold_2G00115300 Rroxscaffold_2G00115330 Rroxscaffold_2G00118520 Rroxscaffold_2G00118830 Rroxscaffold_2G00119890 Rroxscaffold_3G00247870 Rroxscaffold_7G00186360 Rroxscaffold_7G00207860
rosa_rugosa	Rorug02G0280300 Rorug02G0280400 Rorug07G0125400
rosa_samantha	Rh2AG309500 Rh2AG309600 Rh2BG318300 Rh2BG341600 Rh2CG296400 Rh2CG301400 Rh2DG333500 Rh2DG358300 Rh3AG290600 Rh3DG323500 Rh7AG256900 Rh7AG257900 Rh7BG252000 Rh7CG273400 Rh7CG274500 Rh7CG274600 Rh7DG263300 Rh7DG263400 Rh7DG263900 Rh7DG265100 Rh7DG272000
rosa_wichuraiana	Rw2G025010 Rw7G022690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1153
AccB1I	GGYRCC	1 cut(s)	589
AccB7I	CCANNNNNTGG	1 cut(s)	280
AccII	CGCG	1 cut(s)	246
AciI	CCGC	7 cut(s)	288, 305, 324, 327, 330, 444, 551
AclWI	GGATC	4 cut(s)	221, 236, 265, 336
AcoI	YGGCCR	1 cut(s)	855
AcsI	RAATTY	3 cut(s)	424, 487, 604
AcuI	CTGAAG	4 cut(s)	655, 980, 1236, 1364
AcyI	GRCGYC	1 cut(s)	554
AfaI	GTAC	2 cut(s)	131, 934
AfiI	CCNNNNNNNGG	5 cut(s)	192, 280, 361, 367, 1027
AflIII	ACRYGT	1 cut(s)	1150
AgsI	TTSAA	4 cut(s)	424, 1498, 1532, 1634
AhlI	ACTAGT	1 cut(s)	1069
AjnI	CCWGG	2 cut(s)	201, 366
AjuI	GAANNNNNNNTTGG	2 cut(s)	653, 685
Alw21I	GWGCWC	5 cut(s)	38, 185, 395, 465, 636
Alw26I	GTCTC	5 cut(s)	253, 840, 983, 1121, 1159
AlwI	GGATC	4 cut(s)	221, 236, 265, 336
AlwNI	CAGNNNCTG	1 cut(s)	280
Ama87I	CYCGRG	1 cut(s)	238
AoxI	GGCC	6 cut(s)	208, 283, 357, 363, 855, 1089
ApaI	GGGCCC	2 cut(s)	287, 361
ApeKI	GCWGC	4 cut(s)	25, 1183, 1420, 1501
ApoI	RAATTY	3 cut(s)	424, 487, 604
Asp700I	GAANNNNTTC	1 cut(s)	1527
AspLEI	GCGC	2 cut(s)	470, 666
AspS9I	GGNCC	9 cut(s)	208, 283, 284, 357, 358, 364, 1009, 1335, 1394
AsuC2I	CCSGG	5 cut(s)	193, 239, 240, 356, 362
AsuHPI	GGTGA	3 cut(s)	382, 649, 916
AsuII	TTCGAA	1 cut(s)	485
AvaI	CYCGRG	1 cut(s)	238
AvaII	GGWCC	3 cut(s)	1009, 1335, 1394
AxyI	CCTNAGG	1 cut(s)	594
BaeGI	GKGCMC	2 cut(s)	287, 361
BalI	TGGCCA	1 cut(s)	857
BanI	GGYRCC	1 cut(s)	589
BanII	GRGCYC	6 cut(s)	38, 287, 361, 395, 465, 636
BbsI	GAAGAC	2 cut(s)	552, 1056
Bbv12I	GWGCWC	5 cut(s)	38, 185, 395, 465, 636
BbvCI	CCTCAGC	1 cut(s)	1568
BbvI	GCAGC	4 cut(s)	37, 1170, 1407, 1513
BccI	CCATC	2 cut(s)	301, 1020
BcgI	CGANNNNNNTGC	6 cut(s)	391, 425, 1412, 1446, 1607, 1641
BciT130I	CCWGG	2 cut(s)	203, 368
BclI	TGATCA	1 cut(s)	510
BcnI	CCSGG	5 cut(s)	193, 239, 240, 356, 362
BcoDI	GTCTC	5 cut(s)	253, 840, 983, 1121, 1159
BcuI	ACTAGT	1 cut(s)	1069
BfaI	CTAG	2 cut(s)	843, 1070
BfmI	CTRYAG	2 cut(s)	714, 1421
BfoI	RGCGCY	2 cut(s)	471, 667
BfuAI	ACCTGC	1 cut(s)	1153
BglI	GCCNNNNNGGC	1 cut(s)	207
BisI	GCNGC	7 cut(s)	26, 327, 330, 552, 1184, 1421, 1502
BlpI	GCTNAGC	3 cut(s)	464, 1239, 1298
BlsI	GCNGC	7 cut(s)	27, 328, 331, 553, 1185, 1422, 1503
Bme1390I	CCNGG	7 cut(s)	193, 203, 239, 240, 356, 362, 368
Bme18I	GGWCC	3 cut(s)	1009, 1335, 1394
BmeT110I	CYCGRG	1 cut(s)	238
BmgT120I	GGNCC	9 cut(s)	208, 283, 284, 357, 358, 364, 1009, 1335, 1394
BmiI	GGNNCC	9 cut(s)	285, 286, 359, 360, 591, 806, 1336, 1337, 1615
BmrFI	CCNGG	7 cut(s)	193, 203, 239, 240, 356, 362, 368
BmrI	ACTGGG	1 cut(s)	290
BmsI	GCATC	3 cut(s)	729, 823, 928
BmuI	ACTGGG	1 cut(s)	290
BoxI	GACNNNNGTC	1 cut(s)	222
BpiI	GAAGAC	2 cut(s)	552, 1056
Bpu10I	CCTNAGC	1 cut(s)	1568
Bpu1102I	GCTNAGC	3 cut(s)	464, 1239, 1298
Bpu14I	TTCGAA	1 cut(s)	485
BpuEI	CTTGAG	5 cut(s)	64, 479, 725, 782, 1141
BpuMI	CCSGG	5 cut(s)	193, 239, 240, 356, 362
Bsa29I	ATCGAT	1 cut(s)	258
BsaHI	GRCGYC	1 cut(s)	554
BsaI	GGTCTC	1 cut(s)	983
BsaJI	CCNNGG	5 cut(s)	237, 238, 360, 366, 773
BsaXI	ACNNNNNCTCC	7 cut(s)	26, 125, 143, 155, 173, 593, 623
Bsc4I	CCNNNNNNNGG	5 cut(s)	192, 280, 361, 367, 1027
Bse118I	RCCGGY	1 cut(s)	1353
Bse1I	ACTGG	3 cut(s)	285, 1099, 1184
Bse21I	CCTNAGG	1 cut(s)	594
Bse3DI	GCAATG	2 cut(s)	409, 1335
BseBI	CCWGG	2 cut(s)	203, 368
BseCI	ATCGAT	1 cut(s)	258
BseDI	CCNNGG	5 cut(s)	237, 238, 360, 366, 773
BseGI	GGATG	2 cut(s)	652, 1191
BseLI	CCNNNNNNNGG	5 cut(s)	192, 280, 361, 367, 1027
BseMI	GCAATG	2 cut(s)	409, 1335
BseMII	CTCAG	7 cut(s)	470, 478, 585, 771, 1230, 1289, 1582
BseNI	ACTGG	3 cut(s)	285, 1099, 1184
BseRI	GAGGAG	4 cut(s)	288, 291, 806, 1100
BseSI	GKGCMC	2 cut(s)	287, 361
BseXI	GCAGC	4 cut(s)	37, 1170, 1407, 1513
BseYI	CCCAGC	1 cut(s)	167
BsgI	GTGCAG	1 cut(s)	1006
Bsh1236I	CGCG	1 cut(s)	246
BshFI	GGCC	6 cut(s)	210, 285, 359, 365, 857, 1091
BshNI	GGYRCC	1 cut(s)	589
BshVI	ATCGAT	1 cut(s)	258
BsiHKAI	GWGCWC	5 cut(s)	38, 185, 395, 465, 636
BsiHKCI	CYCGRG	1 cut(s)	238
BsiSI	CCGG	7 cut(s)	192, 211, 239, 355, 362, 504, 1354
BslFI	GGGAC	3 cut(s)	149, 220, 1348
BslI	CCNNNNNNNGG	5 cut(s)	192, 280, 361, 367, 1027
BsmAI	GTCTC	5 cut(s)	253, 840, 983, 1121, 1159
BsmBI	CGTCTC	1 cut(s)	253
BsmFI	GGGAC	3 cut(s)	149, 220, 1348
BsnI	GGCC	6 cut(s)	210, 285, 359, 365, 857, 1091
Bso31I	GGTCTC	1 cut(s)	983
BsoBI	CYCGRG	1 cut(s)	238
Bsp119I	TTCGAA	1 cut(s)	485
Bsp120I	GGGCCC	2 cut(s)	283, 357
Bsp1286I	GDGCHC	7 cut(s)	38, 185, 287, 361, 395, 465, 636
Bsp1407I	TGTACA	1 cut(s)	932
Bsp1720I	GCTNAGC	3 cut(s)	464, 1239, 1298
BspACI	CCGC	7 cut(s)	288, 305, 324, 327, 330, 444, 551
BspANI	GGCC	6 cut(s)	210, 285, 359, 365, 857, 1091
BspCNI	CTCAG	7 cut(s)	471, 477, 586, 772, 1231, 1290, 1581
BspDI	ATCGAT	1 cut(s)	258
BspFNI	CGCG	1 cut(s)	246
BspHI	TCATGA	1 cut(s)	1015
BspLI	GGNNCC	9 cut(s)	285, 286, 359, 360, 591, 806, 1336, 1337, 1615
BspMAI	CTGCAG	2 cut(s)	718, 1425
BspMI	ACCTGC	1 cut(s)	1153
BspPI	GGATC	4 cut(s)	221, 236, 265, 336
BspT104I	TTCGAA	1 cut(s)	485
BspT107I	GGYRCC	1 cut(s)	589
BspTNI	GGTCTC	1 cut(s)	983
BsrDI	GCAATG	2 cut(s)	409, 1335
BsrFI	RCCGGY	1 cut(s)	1353
BsrGI	TGTACA	1 cut(s)	932
BsrI	ACTGG	3 cut(s)	285, 1099, 1184
BssAI	RCCGGY	1 cut(s)	1353
BssECI	CCNNGG	5 cut(s)	237, 238, 360, 366, 773
BssNI	GRCGYC	1 cut(s)	554
BssT1I	CCWWGG	1 cut(s)	773
Bst2UI	CCWGG	2 cut(s)	203, 368
Bst4CI	ACNGT	2 cut(s)	316, 1120
Bst6I	CTCTTC	3 cut(s)	431, 674, 783
BstACI	GRCGYC	1 cut(s)	554
BstAPI	GCANNNNNTGC	2 cut(s)	1510, 1557
BstAUI	TGTACA	1 cut(s)	932
BstBI	TTCGAA	1 cut(s)	485
BstC8I	GCNNGC	1 cut(s)	718
BstENI	CCTNNNNNAGG	1 cut(s)	1025
BstF5I	GGATG	2 cut(s)	652, 1191
BstFNI	CGCG	1 cut(s)	246
BstH2I	RGCGCY	2 cut(s)	471, 667
BstHHI	GCGC	2 cut(s)	470, 666
BstMAI	GTCTC	5 cut(s)	253, 840, 983, 1121, 1159
BstMWI	GCNNNNNNNGC	6 cut(s)	207, 390, 399, 1204, 1510, 1557
BstNI	CCWGG	2 cut(s)	203, 368
BstPAI	GACNNNNGTC	1 cut(s)	222
BstSCI	CCNGG	7 cut(s)	191, 201, 237, 238, 354, 360, 366
BstSFI	CTRYAG	2 cut(s)	714, 1421
BstSLI	GKGCMC	2 cut(s)	287, 361
BstUI	CGCG	1 cut(s)	246
BstV1I	GCAGC	4 cut(s)	37, 1170, 1407, 1513
BstV2I	GAAGAC	2 cut(s)	552, 1056
BstX2I	RGATCY	2 cut(s)	228, 341
BstYI	RGATCY	2 cut(s)	228, 341
Bsu15I	ATCGAT	1 cut(s)	258
Bsu36I	CCTNAGG	1 cut(s)	594
BsuRI	GGCC	6 cut(s)	210, 285, 359, 365, 857, 1091
BsuTUI	ATCGAT	1 cut(s)	258
BtsCI	GGATG	2 cut(s)	652, 1191
BtsIMutI	CAGTG	3 cut(s)	520, 1002, 1092
BveI	ACCTGC	1 cut(s)	1153
Cac8I	GCNNGC	1 cut(s)	718
CaiI	CAGNNNCTG	1 cut(s)	280
CciI	TCATGA	1 cut(s)	1015
CfoI	GCGC	2 cut(s)	470, 666
Cfr10I	RCCGGY	1 cut(s)	1353
Cfr13I	GGNCC	9 cut(s)	208, 283, 284, 357, 358, 364, 1009, 1335, 1394
Cfr9I	CCCGGG	1 cut(s)	238
ClaI	ATCGAT	1 cut(s)	258
CseI	GACGC	2 cut(s)	235, 543
Csp6I	GTAC	2 cut(s)	130, 933
CviAII	CATG	8 cut(s)	51, 637, 724, 953, 1016, 1056, 1265, 1386
CviQI	GTAC	2 cut(s)	130, 933
DraI	TTTAAA	1 cut(s)	753
EaeI	YGGCCR	1 cut(s)	855
Eam1104I	CTCTTC	3 cut(s)	431, 674, 783
EarI	CTCTTC	3 cut(s)	431, 674, 783
EciI	GGCGGA	2 cut(s)	294, 313
Ecl136II	GAGCTC	4 cut(s)	36, 393, 463, 634
Eco130I	CCWWGG	1 cut(s)	773
Eco24I	GRGCYC	6 cut(s)	38, 287, 361, 395, 465, 636
Eco31I	GGTCTC	1 cut(s)	983
Eco47I	GGWCC	3 cut(s)	1009, 1335, 1394
Eco53kI	GAGCTC	4 cut(s)	36, 393, 463, 634
Eco57I	CTGAAG	4 cut(s)	655, 980, 1236, 1364
Eco81I	CCTNAGG	1 cut(s)	594
Eco88I	CYCGRG	1 cut(s)	238
EcoICRI	GAGCTC	4 cut(s)	36, 393, 463, 634
EcoNI	CCTNNNNNAGG	1 cut(s)	1025
EcoO109I	RGGNCCY	3 cut(s)	284, 358, 1335
EcoRI	GAATTC	1 cut(s)	487
EcoRII	CCWGG	2 cut(s)	201, 366
EcoT14I	CCWWGG	1 cut(s)	773
EcoT22I	ATGCAT	1 cut(s)	1266
EcoT38I	GRGCYC	6 cut(s)	38, 287, 361, 395, 465, 636
ErhI	CCWWGG	1 cut(s)	773
Esp3I	CGTCTC	1 cut(s)	253
FaeI	CATG	8 cut(s)	54, 640, 727, 956, 1019, 1059, 1268, 1389
FalI	AAGNNNNNCTT	4 cut(s)	653, 685, 725, 757
FaqI	GGGAC	3 cut(s)	149, 220, 1348
FatI	CATG	8 cut(s)	50, 636, 723, 952, 1015, 1055, 1264, 1385
FauI	CCCGC	2 cut(s)	295, 437
FbaI	TGATCA	1 cut(s)	510
Fnu4HI	GCNGC	7 cut(s)	26, 327, 330, 552, 1184, 1421, 1502
FokI	GGATG	2 cut(s)	659, 1178
FriOI	GRGCYC	6 cut(s)	38, 287, 361, 395, 465, 636
Fsp4HI	GCNGC	7 cut(s)	26, 327, 330, 552, 1184, 1421, 1502
FspBI	CTAG	2 cut(s)	843, 1070
GlaI	GCGC	2 cut(s)	469, 665
GluI	GCNGC	7 cut(s)	26, 327, 330, 552, 1184, 1421, 1502
GsaI	CCCAGC	1 cut(s)	171
HaeII	RGCGCY	2 cut(s)	471, 667
HaeIII	GGCC	6 cut(s)	210, 285, 359, 365, 857, 1091
HapII	CCGG	7 cut(s)	192, 211, 239, 355, 362, 504, 1354
HgaI	GACGC	2 cut(s)	235, 543
HhaI	GCGC	2 cut(s)	470, 666
Hin1I	GRCGYC	1 cut(s)	554
Hin1II	CATG	8 cut(s)	54, 640, 727, 956, 1019, 1059, 1268, 1389
Hin6I	GCGC	2 cut(s)	468, 664
HinP1I	GCGC	2 cut(s)	468, 664
HinfI	GANTC	7 cut(s)	89, 260, 320, 875, 884, 1361, 1634
HpaII	CCGG	7 cut(s)	192, 211, 239, 355, 362, 504, 1354
HphI	GGTGA	3 cut(s)	382, 649, 916
Hpy166II	GTNNAC	2 cut(s)	729, 813
Hpy188I	TCNGA	9 cut(s)	228, 397, 480, 493, 1113, 1360, 1434, 1476, 1618
Hpy188III	TCNNGA	9 cut(s)	15, 458, 673, 761, 903, 1016, 1365, 1582, 1638
Hpy8I	GTNNAC	2 cut(s)	729, 813
Hpy99I	CGWCG	8 cut(s)	21, 48, 228, 320, 476, 547, 550, 559
HpyAV	CCTTC	5 cut(s)	103, 1000, 1117, 1429, 1588
HpyCH4III	ACNGT	2 cut(s)	316, 1120
HpyCH4IV	ACGT	2 cut(s)	474, 1150
HpyF10VI	GCNNNNNNNGC	6 cut(s)	207, 390, 399, 1204, 1510, 1557
HpySE526I	ACGT	2 cut(s)	474, 1150
Hsp92I	GRCGYC	1 cut(s)	554
Hsp92II	CATG	8 cut(s)	54, 640, 727, 956, 1019, 1059, 1268, 1389
HspAI	GCGC	2 cut(s)	468, 664
KflI	GGGWCCC	1 cut(s)	1335
Ksp22I	TGATCA	1 cut(s)	510
LmnI	GCTCC	4 cut(s)	176, 398, 493, 1619
Lsp1109I	GCAGC	4 cut(s)	37, 1170, 1407, 1513
LweI	GCATC	3 cut(s)	729, 823, 928
MaeI	CTAG	2 cut(s)	843, 1070
MaeII	ACGT	2 cut(s)	474, 1150
MboII	GAAGA	9 cut(s)	448, 552, 661, 797, 800, 1043, 1056, 1229, 1370
MflI	RGATCY	2 cut(s)	228, 341
MhlI	GDGCHC	7 cut(s)	38, 185, 287, 361, 395, 465, 636
MlsI	TGGCCA	1 cut(s)	857
MluCI	AATT	5 cut(s)	424, 487, 582, 604, 1314
MluNI	TGGCCA	1 cut(s)	857
MlyI	GAGTC	2 cut(s)	869, 1370
MmeI	TCCRAC	4 cut(s)	123, 180, 206, 1412
Mox20I	TGGCCA	1 cut(s)	857
Mph1103I	ATGCAT	1 cut(s)	1266
MroXI	GAANNNNTTC	1 cut(s)	1527
MscI	TGGCCA	1 cut(s)	857
MseI	TTAA	3 cut(s)	752, 822, 1257
Msp20I	TGGCCA	1 cut(s)	857
MspA1I	CMGCKG	1 cut(s)	28
MspI	CCGG	7 cut(s)	192, 211, 239, 355, 362, 504, 1354
MspR9I	CCNGG	7 cut(s)	193, 203, 239, 240, 356, 362, 368
MvaI	CCWGG	2 cut(s)	203, 368
MvnI	CGCG	1 cut(s)	246
MwoI	GCNNNNNNNGC	6 cut(s)	207, 390, 399, 1204, 1510, 1557
NciI	CCSGG	5 cut(s)	193, 239, 240, 356, 362
NlaIII	CATG	8 cut(s)	54, 640, 727, 956, 1019, 1059, 1268, 1389
NlaIV	GGNNCC	9 cut(s)	285, 286, 359, 360, 591, 806, 1336, 1337, 1615
NmeAIII	GCCGAG	2 cut(s)	403, 1328
NsiI	ATGCAT	1 cut(s)	1266
NspV	TTCGAA	1 cut(s)	485
PagI	TCATGA	1 cut(s)	1015
PcsI	WCGNNNNNNNCGW	2 cut(s)	551, 554
PdmI	GAANNNNTTC	1 cut(s)	1527
PfeI	GAWTC	5 cut(s)	89, 260, 320, 884, 1634
PflFI	GACNNNGTC	1 cut(s)	878
PflMI	CCANNNNNTGG	1 cut(s)	280
PkrI	GCNGC	7 cut(s)	27, 328, 331, 553, 1185, 1422, 1503
PleI	GAGTC	2 cut(s)	869, 1369
PpsI	GAGTC	2 cut(s)	869, 1369
PpuMI	RGGWCCY	1 cut(s)	1335
PshAI	GACNNNNGTC	1 cut(s)	222
Psp124BI	GAGCTC	4 cut(s)	38, 395, 465, 636
Psp5II	RGGWCCY	1 cut(s)	1335
Psp6I	CCWGG	2 cut(s)	201, 366
PspFI	CCCAGC	1 cut(s)	167
PspGI	CCWGG	2 cut(s)	201, 366
PspN4I	GGNNCC	9 cut(s)	285, 286, 359, 360, 591, 806, 1336, 1337, 1615
PspOMI	GGGCCC	2 cut(s)	283, 357
PspPI	GGNCC	9 cut(s)	208, 283, 284, 357, 358, 364, 1009, 1335, 1394
PspPPI	RGGWCCY	1 cut(s)	1335
PstI	CTGCAG	2 cut(s)	718, 1425
PstNI	CAGNNNCTG	1 cut(s)	280
PsuI	RGATCY	2 cut(s)	228, 341
PsyI	GACNNNGTC	1 cut(s)	878
PvuII	CAGCTG	1 cut(s)	28
RsaI	GTAC	2 cut(s)	131, 934
RsaNI	GTAC	2 cut(s)	130, 933
SacI	GAGCTC	4 cut(s)	38, 395, 465, 636
SaqAI	TTAA	3 cut(s)	752, 822, 1257
SatI	GCNGC	7 cut(s)	26, 327, 330, 552, 1184, 1421, 1502
Sau96I	GGNCC	9 cut(s)	208, 283, 284, 357, 358, 364, 1009, 1335, 1394
SchI	GAGTC	2 cut(s)	869, 1370
ScrFI	CCNGG	7 cut(s)	193, 203, 239, 240, 356, 362, 368
SduI	GDGCHC	7 cut(s)	38, 185, 287, 361, 395, 465, 636
SfaNI	GCATC	3 cut(s)	729, 823, 928
SfcI	CTRYAG	2 cut(s)	714, 1421
SfuI	TTCGAA	1 cut(s)	485
SinI	GGWCC	3 cut(s)	1009, 1335, 1394
SmaI	CCCGGG	1 cut(s)	240
SmlI	CTYRAG	5 cut(s)	79, 458, 704, 761, 1156
SmoI	CTYRAG	5 cut(s)	79, 458, 704, 761, 1156
SpeI	ACTAGT	1 cut(s)	1069
Sse9I	AATT	5 cut(s)	424, 487, 582, 604, 1314
SsiI	CCGC	7 cut(s)	288, 305, 324, 327, 330, 444, 551
SspI	AATATT	1 cut(s)	1535
SspMI	CTAG	2 cut(s)	843, 1070
SstI	GAGCTC	4 cut(s)	38, 395, 465, 636
StyD4I	CCNGG	7 cut(s)	191, 201, 237, 238, 354, 360, 366
StyI	CCWWGG	1 cut(s)	773
TaaI	ACNGT	2 cut(s)	316, 1120
TaiI	ACGT	2 cut(s)	477, 1153
TasI	AATT	5 cut(s)	424, 487, 582, 604, 1314
TatI	WGTACW	1 cut(s)	932
TauI	GCSGC	3 cut(s)	329, 332, 554
TfiI	GAWTC	5 cut(s)	89, 260, 320, 884, 1634
Tru1I	TTAA	3 cut(s)	752, 822, 1257
Tru9I	TTAA	3 cut(s)	752, 822, 1257
TscAI	CASTG	3 cut(s)	520, 1002, 1099
TseI	GCWGC	4 cut(s)	25, 1183, 1420, 1501
TspDTI	ATGAA	1 cut(s)	906
TspGWI	ACGGA	1 cut(s)	151
TspMI	CCCGGG	1 cut(s)	238
TspRI	CASTG	3 cut(s)	520, 1002, 1099
Tth111I	GACNNNGTC	1 cut(s)	878
Van91I	CCANNNNNTGG	1 cut(s)	280
VpaK11BI	GGWCC	3 cut(s)	1009, 1335, 1394
XagI	CCTNNNNNAGG	1 cut(s)	1025
XapI	RAATTY	3 cut(s)	424, 487, 604
XmaI	CCCGGG	1 cut(s)	238
XmnI	GAANNNNTTC	1 cut(s)	1527
XspI	CTAG	2 cut(s)	843, 1070
Zsp2I	ATGCAT	1 cut(s)	1266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.