Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0004791

Physical Location & Seq

Genomic Coordinates Forward (+)

5418739 .. 5423905

Gene Structure 5167 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0062400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2901 bp

ATGATGGCTAAGCCACAGTTCAAAGATCAGAGAGAAATACAAAAAATGGAGGTTGCAAGCAAAGAGAATCAGCTGTGGGCACTGATCCATGCCAAAGGTCTTTTGCACTCTGATGTTCAGGACTTGTACCGGAAAGTGAGGTCTTGTTATGAGAATATCATCCTGAATGATAATGCACAGCTGGAACTTCAAGACATTGAGTATTCTTTGTGGAAGCTTTACTATAAGCTTATAGATGAATTTCGTAAAAGAATAAAGAAAAGTTCAGCAGCGCCTCAACATGATAGTCATATAGAAGGATTAAAACTCTTTCTATCTGAAGGAATTCAGTTCTACCAAAATTTGATTGTGAAAATAAGAGAATGCTATGGCCTCACAGAAGAGTCTGTGTTCTACAGAAAGGGAGGCATAATTACCCTTGGGGAACAAAAGAAGATGCAGAAATGCCAATTTTTGTGCCATCGCTTTTTGGTATGCCTCGGGGATCTTGCTAGATATAAAGAACAATATGAAAAACCAGATGTCCATAGCCGAAATTGGTCAGTTGCAGCCACCCACTACTTGGAAGCAACAAGAATTTGGCCAGATAGTGGAAACCCCCAAAATCAGTTAGCTGTATTGGCGATGTACATTGGTGATGAATTGCTCGCGTTATACCACTGTATAAGAAGTTTAGCTGTTAAAAATCCCTTTCCTGAGGCCAAGGATAACCTCACCTTACTGTTTGAAAAGAACAGCTCATCTCATTTACATTCTCTTTCAAGCGAGTGCCAATTTAATTTCTTACATCCATCTGAAAGAAGCAGTGTTCAGATTACTAAACAAGAAAGCAATGATAATATGCTGAAAGCTGAAATGGATACAAATTTGTGGTCTCTTATGATCAGGACGCTAAGTTTCCTCCACTTAAAATTGAGTGTGGATCAATTCCCTTGTGCTTTCGCATCTACTATGAGAGAGTTGGATGCATTGATGGCACTAGATGATACAAAACTAAATGCTACATTGGAGTCATATCAGCGTATGGATTCAGTTAGACGAGGCCCTTATCGAGTGCTCCAGGTTGTTTCTGTACTGATCTTTATCATACAGAATCTAGTTAAGATCCCAGAAACAAAAGACTCGAAGAAGCAAAATGATATGCAGCACATGGAGCTGACACAGTTGGGATTGACTGCCACATTCATATTCATGGGACGCTTTGTTGAAAGATGTTTGCAGGCAAGTACAATCGAGACATGCCCCCTTCTACCAGCTGTGCTTGTTTTTGTGGAATGGTTGGTCTTCATTCTTGATGAAGCAGAAACGTATGGGGTTGATGAGAAAAGCAAAAGTGCTATGTCCTACTTTTTTGGTGAATTTGTTGATCTTCTAAAGCAATTGAATGTCCATGTCGGTGAGGCCAAGTGTCCAGCAGGTGCTTCTCTCTGGGAAGACTATGAGCTGAGGGGCTTTGCACCAGTAGCTCAATCACATGCATTACTAGATTTTTCATCTCATTGGGAACATATAGACAATTATGAAAGTGGAATGGATTGTCGTTCTCAGCGTATCATCGATGCCGCAATTAAAATTGCAAGCAGATCAACTGATTCTCAAAAGTGGATTGTCTATGACAAGTCTGGAAGCAAATTTTGCACATACCACATGACAGAGTCAAATGGATATCTGGACAGGAAAGAGTCAGGAAGATTGGAGACTAACAACTCCGATGTTGAATTGAAAGTGTCTAGTCAGAAGATTGACAAAGCACCAGAAGAATGTGAGAAACCGATGAGTGATGGGGAAAATCTAAGCTCTATTGCTGTGGAAGAGGAAGAAGTTATTCTTTTCAGGCCTCTTACTAGACGTAATTCAGCACCAGTCAGTATGGCCTCTGCTTTAAAGGACCCAACCTCTCCAAAACATTCATTGGATCAAAAGGTGCCTTCTGATGAATGTTTGCGCCGTGCTACGTCACTTCTTATAGCACAAAACCCGGCTCAGAGTGATCCTTTTTCTTTCCATACTGACATGACTCATTTTGGGCGCAACATGTCATATAAGCAGCAGCAGCCTGTTTTCACAGATACAATGGCACAGCCATTTCCGGAAACTCCAATCACCGCTGGCCCTCCCTCCCTTAGTGCTTGGGTCTTTGATAGGGGCAGTTTGAGCAATAGTAGGGAGAAAAGCACACATGGGACAAGTAAACATGGATCATCAAGGTTGAGCCCTATTGAGGAAGTAGCTTCAGAATCTTTGATTGGCCTATCATTAAGTGACAATGAAGATTCTTTCAGCCATCACGAATTTGCAAGCACCTTATCCTATCCGTCTTCTTATACAGCTCCTGTGCCTTCAGCCCCTCCTTTATTGCCAGATGATGAGCCCCTTTGGTTTAATAAGGGGATCAGCATGACAGATAATGCTTCTGATGTATCATATTCACAAGCAAGCAGCTATCCACATTGGACTGCTACTCAAGGGCCACCTCATTTCAGTCCCATCATTCCGAGTTTCGTGGACAAATACCCAACTGCACATAGAATGACTTCATCTGAATGGCTTCGTCAGTACAGAGAAAGCCATAACCTTGAGCATCATGGTGGGCCCAATTATGTTCATCCTCCCAGTAACCTTGGAAACTTGTACGGTTATGATACTCCAAGGTTTCATCATTTCAATCAACGGGGAAATCTTGCGGCTTCTAATCCGGCAATGCGTATAGATGACTCGACATTGTATCCAGGTTTCCCTCTAGATCCAGGTTTCTCTGGTTACCAAAGAACAAGCCTCTATGGGTGTGGTGCTGTGACAGACCTAAGAAATGAGCAGCAACCACTTCTTCATTACCTTAAGGAGAGAGAGAAGCAACTCCAGAGAGATCCAACTGTGAGAGGTCCATATATGGACAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004791 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009636 GO:0009987 GO:0015035 GO:0015036 GO:0016209 GO:0016491 GO:0016651 GO:0016667 GO:0016668 GO:0016671 GO:0019725 GO:0031974 GO:0033554 GO:0034599 GO:0042221 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045454 GO:0047134 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0097237 GO:0098754 GO:0098869 GO:1990748

KEGG Pathways

Metabolic & Signaling

ko04621 ko05418 map04621 map05418

Pfam Domains

Protein Families

Thioredoxin

Protein Analysis

966

Amino Acids

109.93

Weight (kDa)

5.9

Isoelectric Point (pI)

46.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EST1	PF10374	66 - 171	1.9e-15	Telomerase activating protein Est1
EST1_DNA_bind	PF10373	183 - 491	7.5e-56	Est1 DNA/RNA binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004791)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31020 AT2G35010 AT2G35010
fragaria_vesca	FvH4_3g12700 FvH4_3g12700 FvH4_3g12700
malus_domestica	MD05G1092600.v1.1 MD05G1346300.v1.1 MD10G1228900.v1.1 MD10G1322700.v1.1 MD10G1338300.v1.1 MD15G1415400.v1.1 MD17G1134000.v1.1
prunus_persica	Prupe.4G115800_v2.0.a1 Prupe.4G115800_v2.0.a1
pyrus_communis	pycom04g16220 pycom05g22440 pycom10g19250 pycom15g10290
rosa_chinensis	RchiOBHm_Chr5g0020681
rosa_laevigata	RLG00000032553
rosa_multiflora	Rmu_sc0005607.1_g000027
rosa_roxburghii	Rroxscaffold_1G00057610
rosa_rugosa	Rorug05G0062400 Rorug05G0062400 Rorug05G0062400
rosa_samantha	Rh5AG152100 Rh5BG151800 Rh5CG163200 Rh5DG151100
rosa_wichuraiana	Rw5G013480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1406
Acc36I	ACCTGC	1 cut(s)	1406
AccB1I	GGYRCC	1 cut(s)	1924
AccB7I	CCANNNNNTGG	2 cut(s)	562, 590
AccII	CGCG	1 cut(s)	650
AccIII	TCCGGA	1 cut(s)	2089
AciI	CCGC	3 cut(s)	1563, 2106, 2684
AcoI	YGGCCR	1 cut(s)	581
AcsI	RAATTY	8 cut(s)	239, 324, 340, 576, 865, 1358, 1631, 2291
AcuI	CTGAAG	3 cut(s)	339, 2217, 2325
AfaI	GTAC	6 cut(s)	128, 629, 1074, 1228, 2558, 2633
AfiI	CCNNNNNNNGG	3 cut(s)	562, 590, 2221
AflII	CTTAAG	1 cut(s)	2837
AflIII	ACRYGT	1 cut(s)	2034
AgsI	TTSAA	9 cut(s)	22, 191, 728, 762, 1208, 1384, 1718, 1723, 2665
AjnI	CCWGG	3 cut(s)	1059, 2728, 2746
AjuI	GAANNNNNNNTTGG	2 cut(s)	2229, 2261
Alw21I	GWGCWC	1 cut(s)	1059
Alw26I	GTCTC	3 cut(s)	879, 1229, 1691
AlwNI	CAGNNNCTG	1 cut(s)	2333
Ama87I	CYCGRG	1 cut(s)	479
Aor13HI	TCCGGA	1 cut(s)	2089
ApaI	GGGCCC	1 cut(s)	2595
ApeKI	GCWGC	8 cut(s)	269, 548, 1144, 2047, 2050, 2053, 2439, 2815
ApoI	RAATTY	8 cut(s)	239, 324, 340, 576, 865, 1358, 1631, 2291
ArsI	GACNNNNNNTTYG	2 cut(s)	857, 889
Asp700I	GAANNNNTTC	4 cut(s)	324, 1824, 2533, 2547
AspLEI	GCGC	3 cut(s)	274, 1947, 2031
AspS9I	GGNCC	7 cut(s)	1043, 1888, 2111, 2468, 2591, 2592, 2882
AsuC2I	CCSGG	1 cut(s)	1979
AsuHPI	GGTGA	5 cut(s)	647, 706, 1367, 1409, 2095
AvaI	CYCGRG	1 cut(s)	479
AvaII	GGWCC	2 cut(s)	1888, 2882
AxyI	CCTNAGG	1 cut(s)	696
BaeGI	GKGCMC	2 cut(s)	82, 2595
BalI	TGGCCA	1 cut(s)	583
BanI	GGYRCC	1 cut(s)	1924
BanII	GRGCYC	3 cut(s)	2216, 2373, 2595
BbsI	GAAGAC	3 cut(s)	1276, 1440, 2310
Bbv12I	GWGCWC	1 cut(s)	1059
BbvCI	CCTCAGC	1 cut(s)	1445
BbvI	GCAGC	8 cut(s)	281, 560, 1156, 2059, 2062, 2065, 2451, 2827
BccI	CCATC	6 cut(s)	468, 799, 967, 1775, 2292, 2495
BceAI	ACGGC	1 cut(s)	1932
BciT130I	CCWGG	3 cut(s)	1061, 2730, 2748
BciVI	GTATCC	2 cut(s)	853, 2736
BclI	TGATCA	1 cut(s)	882
BcnI	CCSGG	1 cut(s)	1979
BcoDI	GTCTC	3 cut(s)	879, 1229, 1691
BfaI	CTAG	7 cut(s)	492, 980, 1097, 1484, 1731, 1845, 2741
BfmI	CTRYAG	1 cut(s)	394
BfoI	RGCGCY	1 cut(s)	275
BfrI	CTTAAG	1 cut(s)	2837
BfuAI	ACCTGC	1 cut(s)	1406
BfuI	GTATCC	2 cut(s)	853, 2736
BlpI	GCTNAGC	1 cut(s)	9
Bme1390I	CCNGG	4 cut(s)	1061, 1979, 2730, 2748
Bme18I	GGWCC	2 cut(s)	1888, 2882
BmeT110I	CYCGRG	1 cut(s)	479
BmgT120I	GGNCC	7 cut(s)	1043, 1888, 2111, 2468, 2591, 2592, 2882
BmiI	GGNNCC	3 cut(s)	1890, 1926, 2593
BmrFI	CCNGG	4 cut(s)	1061, 1979, 2730, 2748
BmrI	ACTGGG	1 cut(s)	2607
BmsI	GCATC	5 cut(s)	426, 953, 955, 1549, 2590
BmuI	ACTGGG	1 cut(s)	2607
BpiI	GAAGAC	3 cut(s)	1276, 1440, 2310
BpmI	CTGGAG	2 cut(s)	1043, 2843
Bpu10I	CCTNAGC	1 cut(s)	1445
Bpu1102I	GCTNAGC	1 cut(s)	9
BpuEI	CTTGAG	2 cut(s)	2448, 2597
BpuMI	CCSGG	1 cut(s)	1979
Bsa29I	ATCGAT	1 cut(s)	1557
BsaBI	GATNNNNATC	1 cut(s)	1082
BsaI	GGTCTC	1 cut(s)	879
BsaJI	CCNNGG	5 cut(s)	418, 478, 702, 2620, 2648
BsaWI	WCCGGW	2 cut(s)	129, 2089
Bsc4I	CCNNNNNNNGG	3 cut(s)	562, 590, 2221
Bse1I	ACTGG	3 cut(s)	1460, 1862, 2613
Bse21I	CCTNAGG	1 cut(s)	696
Bse3DI	GCAATG	2 cut(s)	838, 2706
Bse8I	GATNNNNATC	1 cut(s)	1082
BseAI	TCCGGA	1 cut(s)	2089
BseBI	CCWGG	3 cut(s)	1061, 2730, 2748
BseCI	ATCGAT	1 cut(s)	1557
BseDI	CCNNGG	5 cut(s)	418, 478, 702, 2620, 2648
BseGI	GGATG	4 cut(s)	159, 787, 970, 2605
BseJI	GATNNNNATC	1 cut(s)	1082
BseLI	CCNNNNNNNGG	3 cut(s)	562, 590, 2221
BseMI	GCAATG	2 cut(s)	838, 2706
BseMII	CTCAG	4 cut(s)	687, 1436, 1559, 1997
BseNI	ACTGG	3 cut(s)	1460, 1862, 2613
BseSI	GKGCMC	2 cut(s)	82, 2595
BseXI	GCAGC	8 cut(s)	281, 560, 1156, 2059, 2062, 2065, 2451, 2827
BsgI	GTGCAG	1 cut(s)	2505
Bsh1236I	CGCG	1 cut(s)	650
BshNI	GGYRCC	1 cut(s)	1924
BshVI	ATCGAT	1 cut(s)	1557
BsiHKAI	GWGCWC	1 cut(s)	1059
BsiHKCI	CYCGRG	1 cut(s)	479
BsiSI	CCGG	4 cut(s)	130, 1979, 2090, 2696
BslFI	GGGAC	3 cut(s)	1209, 2197, 2469
BslI	CCNNNNNNNGG	3 cut(s)	562, 590, 2221
BsmAI	GTCTC	3 cut(s)	879, 1229, 1691
BsmFI	GGGAC	3 cut(s)	1209, 2197, 2469
BsmI	GAATGC	1 cut(s)	368
Bso31I	GGTCTC	1 cut(s)	879
BsoBI	CYCGRG	1 cut(s)	479
Bsp120I	GGGCCC	1 cut(s)	2591
Bsp1286I	GDGCHC	5 cut(s)	82, 1059, 2216, 2373, 2595
Bsp13I	TCCGGA	1 cut(s)	2089
Bsp1407I	TGTACA	1 cut(s)	627
Bsp1720I	GCTNAGC	1 cut(s)	9
BspACI	CCGC	3 cut(s)	1563, 2106, 2684
BspCNI	CTCAG	4 cut(s)	688, 1437, 1558, 1996
BspDI	ATCGAT	1 cut(s)	1557
BspEI	TCCGGA	1 cut(s)	2089
BspFNI	CGCG	1 cut(s)	650
BspLI	GGNNCC	3 cut(s)	1890, 1926, 2593
BspMI	ACCTGC	1 cut(s)	1406
BspT107I	GGYRCC	1 cut(s)	1924
BspTI	CTTAAG	1 cut(s)	2837
BspTNI	GGTCTC	1 cut(s)	879
BsrDI	GCAATG	2 cut(s)	838, 2706
BsrGI	TGTACA	1 cut(s)	627
BsrI	ACTGG	3 cut(s)	1460, 1862, 2613
BssECI	CCNNGG	5 cut(s)	418, 478, 702, 2620, 2648
BssT1I	CCWWGG	4 cut(s)	418, 702, 2620, 2648
Bst2UI	CCWGG	3 cut(s)	1061, 2730, 2748
Bst4CI	ACNGT	6 cut(s)	18, 662, 723, 1164, 2636, 2875
Bst6I	CTCTTC	2 cut(s)	375, 1806
BstAFI	CTTAAG	1 cut(s)	2837
BstAPI	GCANNNNNTGC	1 cut(s)	1635
BstAUI	TGTACA	1 cut(s)	627
BstC8I	GCNNGC	7 cut(s)	58, 648, 1221, 1579, 2110, 2299, 2437
BstDEI	CTNAG	9 cut(s)	9, 696, 893, 1445, 1545, 1793, 1983, 2123, 2804
BstEII	GGTNACC	1 cut(s)	2759
BstF5I	GGATG	4 cut(s)	159, 787, 970, 2605
BstFNI	CGCG	1 cut(s)	650
BstH2I	RGCGCY	1 cut(s)	275
BstHHI	GCGC	3 cut(s)	274, 1947, 2031
BstMAI	GTCTC	3 cut(s)	879, 1229, 1691
BstMWI	GCNNNNNNNGC	7 cut(s)	620, 974, 1153, 1463, 1635, 2053, 2154
BstNI	CCWGG	3 cut(s)	1061, 2730, 2748
BstNSI	RCATGY	3 cut(s)	1242, 1478, 2038
BstPI	GGTNACC	1 cut(s)	2759
BstSCI	CCNGG	4 cut(s)	1059, 1977, 2728, 2746
BstSFI	CTRYAG	1 cut(s)	394
BstSLI	GKGCMC	2 cut(s)	82, 2595
BstUI	CGCG	1 cut(s)	650
BstV1I	GCAGC	8 cut(s)	281, 560, 1156, 2059, 2062, 2065, 2451, 2827
BstV2I	GAAGAC	3 cut(s)	1276, 1440, 2310
BstX2I	RGATCY	4 cut(s)	484, 1104, 2743, 2866
BstYI	RGATCY	4 cut(s)	484, 1104, 2743, 2866
Bsu15I	ATCGAT	1 cut(s)	1557
Bsu36I	CCTNAGG	1 cut(s)	696
BsuI	GTATCC	2 cut(s)	853, 2736
BsuTUI	ATCGAT	1 cut(s)	1557
BtgZI	GCGATG	2 cut(s)	446, 638
BtsCI	GGATG	4 cut(s)	159, 787, 970, 2605
BtsI	GCAGTG	1 cut(s)	811
BtsIMutI	CAGTG	3 cut(s)	80, 658, 811
BveI	ACCTGC	1 cut(s)	1406
Cac8I	GCNNGC	7 cut(s)	58, 648, 1221, 1579, 2110, 2299, 2437
CaiI	CAGNNNCTG	1 cut(s)	2333
CfoI	GCGC	3 cut(s)	274, 1947, 2031
Cfr13I	GGNCC	7 cut(s)	1043, 1888, 2111, 2468, 2591, 2592, 2882
ClaI	ATCGAT	1 cut(s)	1557
CseI	GACGC	2 cut(s)	898, 1206
Csp6I	GTAC	6 cut(s)	127, 628, 1073, 1227, 2557, 2632
CviQI	GTAC	6 cut(s)	127, 628, 1073, 1227, 2557, 2632
DdeI	CTNAG	9 cut(s)	9, 696, 893, 1445, 1545, 1793, 1983, 2123, 2804
DraI	TTTAAA	1 cut(s)	1884
EaeI	YGGCCR	1 cut(s)	581
Eam1104I	CTCTTC	2 cut(s)	375, 1806
EarI	CTCTTC	2 cut(s)	375, 1806
Eco130I	CCWWGG	4 cut(s)	418, 702, 2620, 2648
Eco147I	AGGCCT	1 cut(s)	1837
Eco24I	GRGCYC	3 cut(s)	2216, 2373, 2595
Eco31I	GGTCTC	1 cut(s)	879
Eco32I	GATATC	1 cut(s)	1667
Eco47I	GGWCC	2 cut(s)	1888, 2882
Eco57I	CTGAAG	3 cut(s)	339, 2217, 2325
Eco81I	CCTNAGG	1 cut(s)	696
Eco88I	CYCGRG	1 cut(s)	479
Eco91I	GGTNACC	1 cut(s)	2759
EcoO109I	RGGNCCY	2 cut(s)	1043, 1888
EcoO65I	GGTNACC	1 cut(s)	2759
EcoRI	GAATTC	1 cut(s)	324
EcoRII	CCWGG	3 cut(s)	1059, 2728, 2746
EcoRV	GATATC	1 cut(s)	1667
EcoT14I	CCWWGG	4 cut(s)	418, 702, 2620, 2648
EcoT22I	ATGCAT	2 cut(s)	970, 1480
EcoT38I	GRGCYC	3 cut(s)	2216, 2373, 2595
ErhI	CCWWGG	4 cut(s)	418, 702, 2620, 2648
FalI	AAGNNNNNCTT	2 cut(s)	1812, 1844
FaqI	GGGAC	3 cut(s)	1209, 2197, 2469
FbaI	TGATCA	1 cut(s)	882
FokI	GGATG	4 cut(s)	146, 774, 977, 2592
FriOI	GRGCYC	3 cut(s)	2216, 2373, 2595
FspBI	CTAG	7 cut(s)	492, 980, 1097, 1484, 1731, 1845, 2741
GlaI	GCGC	3 cut(s)	273, 1946, 2030
GsuI	CTGGAG	2 cut(s)	1043, 2843
HaeII	RGCGCY	1 cut(s)	275
HapII	CCGG	4 cut(s)	130, 1979, 2090, 2696
HgaI	GACGC	2 cut(s)	898, 1206
HhaI	GCGC	3 cut(s)	274, 1947, 2031
Hin6I	GCGC	3 cut(s)	272, 1945, 2029
HinP1I	GCGC	3 cut(s)	272, 1945, 2029
HindIII	AAGCTT	2 cut(s)	215, 227
HpaII	CCGG	4 cut(s)	130, 1979, 2090, 2696
HphI	GGTGA	5 cut(s)	647, 706, 1367, 1409, 2095
Hpy166II	GTNNAC	2 cut(s)	2192, 2506
Hpy8I	GTNNAC	2 cut(s)	2192, 2506
HpyAV	CCTTC	5 cut(s)	290, 314, 1256, 1938, 2349
HpyCH4III	ACNGT	6 cut(s)	18, 662, 723, 1164, 2636, 2875
HpyCH4IV	ACGT	3 cut(s)	1307, 1849, 1955
HpyF10VI	GCNNNNNNNGC	7 cut(s)	620, 974, 1153, 1463, 1635, 2053, 2154
HpyF3I	CTNAG	9 cut(s)	9, 696, 893, 1445, 1545, 1793, 1983, 2123, 2804
HpySE526I	ACGT	3 cut(s)	1307, 1849, 1955
HspAI	GCGC	3 cut(s)	272, 1945, 2029
Kpn2I	TCCGGA	1 cut(s)	2089
Ksp22I	TGATCA	1 cut(s)	882
LmnI	GCTCC	3 cut(s)	1062, 1153, 2335
Lsp1109I	GCAGC	8 cut(s)	281, 560, 1156, 2059, 2062, 2065, 2451, 2827
LweI	GCATC	5 cut(s)	426, 953, 955, 1549, 2590
MaeI	CTAG	7 cut(s)	492, 980, 1097, 1484, 1731, 1845, 2741
MaeII	ACGT	3 cut(s)	1307, 1849, 1955
MaeIII	GTNAC	5 cut(s)	1956, 2261, 2615, 2759, 2794
MfeI	CAATTG	2 cut(s)	1379, 2896
MflI	RGATCY	4 cut(s)	484, 1104, 2743, 2866
MhlI	GDGCHC	5 cut(s)	82, 1059, 2216, 2373, 2595
MlsI	TGGCCA	1 cut(s)	583
MluNI	TGGCCA	1 cut(s)	583
MlyI	GAGTC	7 cut(s)	392, 1019, 1115, 1664, 1691, 2011, 2708
MmeI	TCCRAC	2 cut(s)	942, 2894
Mox20I	TGGCCA	1 cut(s)	583
Mph1103I	ATGCAT	2 cut(s)	970, 1480
MroI	TCCGGA	1 cut(s)	2089
MroXI	GAANNNNTTC	4 cut(s)	324, 1824, 2533, 2547
MscI	TGGCCA	1 cut(s)	583
MslI	CAYNNNNRTG	5 cut(s)	111, 1190, 1395, 2542, 2586
Msp20I	TGGCCA	1 cut(s)	583
MspA1I	CMGCKG	4 cut(s)	73, 181, 1256, 2108
MspCI	CTTAAG	1 cut(s)	2837
MspI	CCGG	4 cut(s)	130, 1979, 2090, 2696
MspR9I	CCNGG	4 cut(s)	1061, 1979, 2730, 2748
MunI	CAATTG	2 cut(s)	1379, 2896
Mva1269I	GAATGC	1 cut(s)	368
MvaI	CCWGG	3 cut(s)	1061, 2730, 2748
MvnI	CGCG	1 cut(s)	650
MwoI	GCNNNNNNNGC	7 cut(s)	620, 974, 1153, 1463, 1635, 2053, 2154
NciI	CCSGG	1 cut(s)	1979
NlaIV	GGNNCC	3 cut(s)	1890, 1926, 2593
NmuCI	GTSAC	3 cut(s)	1956, 2261, 2794
NsiI	ATGCAT	2 cut(s)	970, 1480
NspI	RCATGY	3 cut(s)	1242, 1478, 2038
PaqCI	CACCTGC	1 cut(s)	1406
PceI	AGGCCT	1 cut(s)	1837
PciI	ACATGT	1 cut(s)	2034
PcsI	WCGNNNNNNNCGW	1 cut(s)	1546
PctI	GAATGC	1 cut(s)	368
PdmI	GAANNNNTTC	4 cut(s)	324, 1824, 2533, 2547
PfeI	GAWTC	6 cut(s)	67, 1028, 1093, 1592, 2237, 2273
PflFI	GACNNNGTC	1 cut(s)	1654
PflMI	CCANNNNNTGG	2 cut(s)	562, 590
PleI	GAGTC	7 cut(s)	391, 1018, 1115, 1663, 1690, 2011, 2708
PpsI	GAGTC	7 cut(s)	391, 1018, 1115, 1663, 1690, 2011, 2708
PpuMI	RGGWCCY	1 cut(s)	1888
PscI	ACATGT	1 cut(s)	2034
Psp5II	RGGWCCY	1 cut(s)	1888
Psp6I	CCWGG	3 cut(s)	1059, 2728, 2746
PspEI	GGTNACC	1 cut(s)	2759
PspGI	CCWGG	3 cut(s)	1059, 2728, 2746
PspN4I	GGNNCC	3 cut(s)	1890, 1926, 2593
PspOMI	GGGCCC	1 cut(s)	2591
PspPI	GGNCC	7 cut(s)	1043, 1888, 2111, 2468, 2591, 2592, 2882
PspPPI	RGGWCCY	1 cut(s)	1888
PstNI	CAGNNNCTG	1 cut(s)	2333
PsuI	RGATCY	4 cut(s)	484, 1104, 2743, 2866
PsyI	GACNNNGTC	1 cut(s)	1654
PvuII	CAGCTG	3 cut(s)	73, 181, 1256
RsaI	GTAC	6 cut(s)	128, 629, 1074, 1228, 2558, 2633
RsaNI	GTAC	6 cut(s)	127, 628, 1073, 1227, 2557, 2632
RseI	CAYNNNNRTG	5 cut(s)	111, 1190, 1395, 2542, 2586
Sau96I	GGNCC	7 cut(s)	1043, 1888, 2111, 2468, 2591, 2592, 2882
SchI	GAGTC	7 cut(s)	392, 1019, 1115, 1664, 1691, 2011, 2708
ScrFI	CCNGG	4 cut(s)	1061, 1979, 2730, 2748
SduI	GDGCHC	5 cut(s)	82, 1059, 2216, 2373, 2595
SfaNI	GCATC	5 cut(s)	426, 953, 955, 1549, 2590
SfcI	CTRYAG	1 cut(s)	394
SinI	GGWCC	2 cut(s)	1888, 2882
SmiMI	CAYNNNNRTG	5 cut(s)	111, 1190, 1395, 2542, 2586
SmlI	CTYRAG	3 cut(s)	2463, 2576, 2837
SmoI	CTYRAG	3 cut(s)	2463, 2576, 2837
SseBI	AGGCCT	1 cut(s)	1837
SsiI	CCGC	3 cut(s)	1563, 2106, 2684
SspMI	CTAG	7 cut(s)	492, 980, 1097, 1484, 1731, 1845, 2741
StuI	AGGCCT	1 cut(s)	1837
StyD4I	CCNGG	4 cut(s)	1059, 1977, 2728, 2746
StyI	CCWWGG	4 cut(s)	418, 702, 2620, 2648
TaaI	ACNGT	6 cut(s)	18, 662, 723, 1164, 2636, 2875
TaiI	ACGT	3 cut(s)	1310, 1852, 1958
TaqI	TCGA	5 cut(s)	1051, 1124, 1233, 1557, 2717
TatI	WGTACW	4 cut(s)	627, 1072, 1226, 2556
TauI	GCSGC	2 cut(s)	1565, 2687
TfiI	GAWTC	6 cut(s)	67, 1028, 1093, 1592, 2237, 2273
TscAI	CASTG	3 cut(s)	87, 665, 811
TseFI	GTSAC	3 cut(s)	1956, 2261, 2794
TseI	GCWGC	8 cut(s)	269, 548, 1144, 2047, 2050, 2053, 2439, 2815
Tsp45I	GTSAC	3 cut(s)	1956, 2261, 2794
TspGWI	ACGGA	1 cut(s)	2304
TspRI	CASTG	3 cut(s)	87, 665, 811
Tth111I	GACNNNGTC	1 cut(s)	1654
Van91I	CCANNNNNTGG	2 cut(s)	562, 590
Vha464I	CTTAAG	1 cut(s)	2837
VpaK11BI	GGWCC	2 cut(s)	1888, 2882
XapI	RAATTY	8 cut(s)	239, 324, 340, 576, 865, 1358, 1631, 2291
XbaI	TCTAGA	1 cut(s)	2740
XceI	RCATGY	3 cut(s)	1242, 1478, 2038
XcmI	CCANNNNNNNNNTGG	1 cut(s)	559
XmnI	GAANNNNTTC	4 cut(s)	324, 1824, 2533, 2547
XspI	CTAG	7 cut(s)	492, 980, 1097, 1484, 1731, 1845, 2741
Zsp2I	ATGCAT	2 cut(s)	970, 1480

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.