Rh5BG151800

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0004791
Physical Location & Seq
Reverse (-)
15500452 .. 15503499
3048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG151800.1

Sequence Viewer

Length: 513 bp
ATGGAAGCAGCAGAGCTCGACACAAAAATGGCGAGAAGAAACTCAGGGCTTCTCCTGGTGCGCCAATTGCTTGCCAATCGCAGCAACCTCTGCGCTGCTCTTCTTCCTCACCACAACCTCCCTTCTTATTCAATCCCAACCCTAAATTCATTCTCAAAGACCCAATCTTCACCTCCTCCCAATCCAAAACCCTTCTCCAATTTCCCAACCTTTCGATCCCTCTCATCATCCTCAGGTGCTCCCTCCAACATTGTAGTCGTTAAGTCTGAGGATGAGTTGAAGAGCGCACTGAGCAAAGCTAAAGATGGGTCTCTGCCGGCGATTTTTTACTTCACTGCAGTGTGGTGCGGGCCGTGCAGGTTCATAGCTCCAGTTATTGGGGAATTGAGTGAGAAGTACCCACATGTGACGACATTTAAGATTGACATTGACGAGGTGTGTGTAGTTGTGTATTGTTTGAATTATGATTTAAGGTCGAAAGAACTGAATTTGTTGGTAGATTATGAAAGGTAG

Protein Analysis

170

Amino Acids

18.84

Weight (kDa)

8.27

Isoelectric Point (pI)

55.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 86 - 146 2.9e-10 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 348
AccB7I CCANNNNNTGG 1 cut(s) 377
AciI CCGC 1 cut(s) 348
AclWI GGATC 1 cut(s) 210
AcsI RAATTY 2 cut(s) 145, 487
AfaI GTAC 1 cut(s) 398
AfiI CCNNNNNNNGG 1 cut(s) 377
AflIII ACRYGT 1 cut(s) 403
AgsI TTSAA 3 cut(s) 132, 280, 460
AjnI CCWGG 1 cut(s) 54
AjuI GAANNNNNNNTTGG 2 cut(s) 130, 162
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 3 cut(s) 16, 299, 368
AluI AGCT 3 cut(s) 16, 299, 368
Alw21I GWGCWC 2 cut(s) 18, 241
Alw26I GTCTC 1 cut(s) 315
AlwI GGATC 1 cut(s) 210
AoxI GGCC 1 cut(s) 350
ApeKI GCWGC 3 cut(s) 8, 81, 95
ApoI RAATTY 2 cut(s) 145, 487
AspLEI GCGC 3 cut(s) 63, 95, 287
AspS9I GGNCC 1 cut(s) 350
AsuHPI GGTGA 2 cut(s) 101, 162
AxyI CCTNAGG 1 cut(s) 232
BanII GRGCYC 1 cut(s) 18
Bbv12I GWGCWC 2 cut(s) 18, 241
BbvI GCAGC 3 cut(s) 20, 82, 93
BccI CCATC 1 cut(s) 299
BceAI ACGGC 1 cut(s) 337
BciT130I CCWGG 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 315
BfmI CTRYAG 1 cut(s) 336
BfuAI ACCTGC 1 cut(s) 348
BisI GCNGC 3 cut(s) 9, 82, 96
BlsI GCNGC 3 cut(s) 10, 83, 97
Bme1390I CCNGG 1 cut(s) 56
BmgT120I GGNCC 1 cut(s) 350
BmrFI CCNGG 1 cut(s) 56
BpmI CTGGAG 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 315
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse118I RCCGGY 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 371
Bse21I CCTNAGG 1 cut(s) 232
BseBI CCWGG 1 cut(s) 56
BseGI GGATG 2 cut(s) 227, 277
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMII CTCAG 4 cut(s) 57, 246, 258, 281
BseNI ACTGG 1 cut(s) 371
BseRI GAGGAG 1 cut(s) 165
BseXI GCAGC 3 cut(s) 20, 82, 93
BsgI GTGCAG 1 cut(s) 376
BshFI GGCC 1 cut(s) 352
BsiHKAI GWGCWC 2 cut(s) 18, 241
BsiSI CCGG 1 cut(s) 317
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 1 cut(s) 315
BsnI GGCC 1 cut(s) 352
Bso31I GGTCTC 1 cut(s) 315
Bsp1286I GDGCHC 2 cut(s) 18, 241
Bsp143I GATC 1 cut(s) 215
BspACI CCGC 1 cut(s) 348
BspANI GGCC 1 cut(s) 352
BspCNI CTCAG 4 cut(s) 56, 245, 259, 282
BspMAI CTGCAG 1 cut(s) 340
BspMI ACCTGC 1 cut(s) 348
BspPI GGATC 1 cut(s) 210
BspQI GCTCTTC 2 cut(s) 105, 275
BspTNI GGTCTC 1 cut(s) 315
BsrFI RCCGGY 1 cut(s) 316
BsrI ACTGG 1 cut(s) 371
BssAI RCCGGY 1 cut(s) 316
BssMI GATC 1 cut(s) 215
Bst2UI CCWGG 1 cut(s) 56
Bst6I CTCTTC 2 cut(s) 105, 275
BstAPI GCANNNNNTGC 1 cut(s) 90
BstC8I GCNNGC 3 cut(s) 72, 318, 350
BstDEI CTNAG 4 cut(s) 43, 232, 267, 290
BstF5I GGATG 2 cut(s) 227, 277
BstHHI GCGC 3 cut(s) 63, 95, 287
BstKTI GATC 1 cut(s) 218
BstMAI GTCTC 1 cut(s) 315
BstMBI GATC 1 cut(s) 215
BstMWI GCNNNNNNNGC 4 cut(s) 67, 90, 291, 354
BstNI CCWGG 1 cut(s) 56
BstNSI RCATGY 1 cut(s) 407
BstSCI CCNGG 1 cut(s) 54
BstSFI CTRYAG 1 cut(s) 336
BstV1I GCAGC 3 cut(s) 20, 82, 93
Bsu36I CCTNAGG 1 cut(s) 232
BsuRI GGCC 1 cut(s) 352
BtsCI GGATG 2 cut(s) 227, 277
BtsI GCAGTG 2 cut(s) 333, 345
BtsIMutI CAGTG 3 cut(s) 287, 333, 345
BveI ACCTGC 1 cut(s) 348
Cac8I GCNNGC 3 cut(s) 72, 318, 350
CfoI GCGC 3 cut(s) 63, 95, 287
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 1 cut(s) 350
Csp6I GTAC 1 cut(s) 397
CviAII CATG 1 cut(s) 404
CviJI RGCY 5 cut(s) 16, 49, 299, 352, 368
CviKI_1 RGCY 5 cut(s) 16, 49, 299, 352, 368
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 4 cut(s) 43, 232, 267, 290
DpnI GATC 1 cut(s) 217
DpnII GATC 1 cut(s) 215
Eam1104I CTCTTC 2 cut(s) 105, 275
EarI CTCTTC 2 cut(s) 105, 275
Ecl136II GAGCTC 1 cut(s) 16
Eco24I GRGCYC 1 cut(s) 18
Eco31I GGTCTC 1 cut(s) 315
Eco53kI GAGCTC 1 cut(s) 16
Eco81I CCTNAGG 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 18
FaeI CATG 1 cut(s) 407
FaiI YATR 4 cut(s) 365, 405, 465, 504
FatI CATG 1 cut(s) 403
FauI CCCGC 1 cut(s) 341
Fnu4HI GCNGC 3 cut(s) 9, 82, 96
FokI GGATG 2 cut(s) 214, 284
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 3 cut(s) 9, 82, 96
GlaI GCGC 3 cut(s) 62, 94, 286
GluI GCNGC 3 cut(s) 9, 82, 96
GsuI CTGGAG 1 cut(s) 354
HaeIII GGCC 1 cut(s) 352
HapII CCGG 1 cut(s) 317
HhaI GCGC 3 cut(s) 63, 95, 287
Hin1II CATG 1 cut(s) 407
Hin6I GCGC 3 cut(s) 61, 93, 285
HinP1I GCGC 3 cut(s) 61, 93, 285
HpaII CCGG 1 cut(s) 317
HphI GGTGA 2 cut(s) 101, 162
Hpy188I TCNGA 1 cut(s) 268
HpyAV CCTTC 2 cut(s) 132, 202
HpyCH4V TGCA 2 cut(s) 338, 357
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 90, 291, 354
HpyF3I CTNAG 4 cut(s) 43, 232, 267, 290
Hsp92II CATG 1 cut(s) 407
HspAI GCGC 3 cut(s) 61, 93, 285
KroI GCCGGC 1 cut(s) 316
KroNI GCCGGC 1 cut(s) 318
Kzo9I GATC 1 cut(s) 215
LguI GCTCTTC 2 cut(s) 105, 275
LmnI GCTCC 2 cut(s) 244, 373
LpnPI CCDG 7 cut(s) 30, 41, 68, 219, 330, 343, 384
Lsp1109I GCAGC 3 cut(s) 20, 82, 93
MaeIII GTNAC 1 cut(s) 406
MalI GATC 1 cut(s) 217
MboI GATC 1 cut(s) 215
MboII GAAGA 5 cut(s) 48, 92, 95, 159, 292
MfeI CAATTG 1 cut(s) 65
MhlI GDGCHC 2 cut(s) 18, 241
MluCI AATT 6 cut(s) 65, 145, 199, 383, 460, 487
MmeI TCCRAC 1 cut(s) 270
MroNI GCCGGC 1 cut(s) 316
MseI TTAA 3 cut(s) 261, 417, 470
MslI CAYNNNNRTG 2 cut(s) 26, 338
MspI CCGG 1 cut(s) 317
MspR9I CCNGG 1 cut(s) 56
MunI CAATTG 1 cut(s) 65
MvaI CCWGG 1 cut(s) 56
MwoI GCNNNNNNNGC 4 cut(s) 67, 90, 291, 354
NaeI GCCGGC 1 cut(s) 318
NdeII GATC 1 cut(s) 215
NgoMIV GCCGGC 1 cut(s) 316
NlaIII CATG 1 cut(s) 407
NmuCI GTSAC 1 cut(s) 406
NspI RCATGY 1 cut(s) 407
OliI CACNNNNGTG 1 cut(s) 338
PciI ACATGT 1 cut(s) 403
PciSI GCTCTTC 2 cut(s) 105, 275
PdiI GCCGGC 1 cut(s) 318
PflMI CCANNNNNTGG 1 cut(s) 377
PkrI GCNGC 3 cut(s) 10, 83, 97
PscI ACATGT 1 cut(s) 403
Psp124BI GAGCTC 1 cut(s) 18
Psp6I CCWGG 1 cut(s) 54
PspGI CCWGG 1 cut(s) 54
PspPI GGNCC 1 cut(s) 350
PstI CTGCAG 1 cut(s) 340
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
RseI CAYNNNNRTG 2 cut(s) 26, 338
SacI GAGCTC 1 cut(s) 18
SapI GCTCTTC 2 cut(s) 105, 275
SaqAI TTAA 3 cut(s) 261, 417, 470
SatI GCNGC 3 cut(s) 9, 82, 96
Sau3AI GATC 1 cut(s) 215
Sau96I GGNCC 1 cut(s) 350
ScrFI CCNGG 1 cut(s) 56
SduI GDGCHC 2 cut(s) 18, 241
SfcI CTRYAG 1 cut(s) 336
SmiMI CAYNNNNRTG 2 cut(s) 26, 338
Sse9I AATT 6 cut(s) 65, 145, 199, 383, 460, 487
SsiI CCGC 1 cut(s) 348
SstI GAGCTC 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 54
TaqI TCGA 3 cut(s) 18, 214, 476
TasI AATT 6 cut(s) 65, 145, 199, 383, 460, 487
Tru1I TTAA 3 cut(s) 261, 417, 470
Tru9I TTAA 3 cut(s) 261, 417, 470
TscAI CASTG 3 cut(s) 294, 340, 345
TseFI GTSAC 1 cut(s) 406
TseI GCWGC 3 cut(s) 8, 81, 95
Tsp45I GTSAC 1 cut(s) 406
TspDTI ATGAA 2 cut(s) 138, 352
TspRI CASTG 3 cut(s) 294, 340, 345
Van91I CCANNNNNTGG 1 cut(s) 377
XapI RAATTY 2 cut(s) 145, 487
XceI RCATGY 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.