Rorug05G0317900

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002309
Physical Location & Seq
Reverse (-)
37787844 .. 37790060
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0317900.1

Sequence Viewer

Length: 678 bp
ATGCCGCTCATCATCAGCTCATCATGTTTTCTTATTCTTGTTTCAGTTTCGAGTATTTATGTTCATGCCTACAAGAACCACACAGTGGGCGACTCCTTGGGTTGGTACGACAACCTCCAAAAGCCCGATCTTAATTACCAGAAATGGGCTGCAGCCAACAACTTCAGTTTGGGAGATTTTCTCATTTTCAATACTGATACGAACCACTCGGTCATCCAAACCTATAACGTCACTACATACAAGCTGTGTGATTACAACAATGCCGTGGAGAATGATACAATCCAATGGTCGGACGCGGAGCCGTCAAACACCGTCCCACATGATGTTTCGGTGGCGGTTCCTTTGTTGAAAGAGGGCATCACATATTTCTTTTCCGGTGATTATGATGGCGAACAGTGCAAGAATGGGCAGCACTTCAAGATAAATGTTACTCACGGACAAGGCTTGCCAAAGACTACAGACAATTCCGCGGCAGGACCAGCAGGCAGTCCACAATCAGGCAATGGTGATGATGAAGCAATCCCGGACACAATCGTCCCTAATTCCAACTTCAATGATCCCAAACCAGATGACAATGATGATGTACAGCCATCTGGAGCCGTTTCATTTTCAGTTCCTCATGTACAAGGCAATCTGATTTTCATTTTGCTCGGTCTTGTCTACTTGTTATGGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_resp B3

Protein Analysis

225

Amino Acids

24.61

Weight (kDa)

4.38

Isoelectric Point (pI)

22.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 40 - 137 2.7e-15 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 533
AccB7I CCANNNNNTGG 1 cut(s) 85
AccBSI CCGCTC 1 cut(s) 7
AccI GTMKAC 1 cut(s) 660
AccII CGCG 2 cut(s) 296, 470
AciI CCGC 5 cut(s) 5, 296, 335, 468, 470
AclWI GGATC 1 cut(s) 551
AcuI CTGAAG 1 cut(s) 148
AdeI CACNNNGTG 1 cut(s) 85
AfaI GTAC 3 cut(s) 107, 585, 624
AfiI CCNNNNNNNGG 5 cut(s) 85, 102, 145, 289, 497
AgsI TTSAA 4 cut(s) 190, 349, 418, 553
AluBI AGCT 2 cut(s) 18, 244
AluI AGCT 2 cut(s) 18, 244
AlwI GGATC 1 cut(s) 551
ApeKI GCWGC 3 cut(s) 149, 152, 409
AspS9I GGNCC 1 cut(s) 476
AsuC2I CCSGG 1 cut(s) 524
AsuHPI GGTGA 2 cut(s) 389, 518
AvaII GGWCC 1 cut(s) 476
BbvI GCAGC 3 cut(s) 136, 164, 421
BccI CCATC 2 cut(s) 380, 598
BceAI ACGGC 3 cut(s) 248, 286, 584
BcnI CCSGG 1 cut(s) 524
BfmI CTRYAG 2 cut(s) 150, 456
BisI GCNGC 5 cut(s) 5, 150, 153, 410, 471
BlsI GCNGC 5 cut(s) 6, 151, 154, 411, 472
Bme1390I CCNGG 1 cut(s) 524
Bme18I GGWCC 1 cut(s) 476
BmgT120I GGNCC 1 cut(s) 476
BmiI GGNNCC 3 cut(s) 300, 339, 598
BmrFI CCNGG 1 cut(s) 524
BmsI GCATC 1 cut(s) 366
BplI GAGNNNNNCTC 2 cut(s) 165, 197
BpmI CTGGAG 1 cut(s) 615
BpuMI CCSGG 1 cut(s) 524
BsaJI CCNNGG 3 cut(s) 96, 264, 468
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 102, 145, 289, 497
Bse3DI GCAATG 1 cut(s) 508
BseDI CCNNGG 3 cut(s) 96, 264, 468
BseGI GGATG 1 cut(s) 213
BseLI CCNNNNNNNGG 5 cut(s) 85, 102, 145, 289, 497
BseMI GCAATG 1 cut(s) 508
BseXI GCAGC 3 cut(s) 136, 164, 421
Bsh1236I CGCG 2 cut(s) 296, 470
BsiSI CCGG 2 cut(s) 375, 524
BslFI GGGAC 2 cut(s) 299, 521
BslI CCNNNNNNNGG 5 cut(s) 85, 102, 145, 289, 497
BsmFI GGGAC 2 cut(s) 299, 521
Bsp1407I TGTACA 2 cut(s) 583, 622
Bsp143I GATC 2 cut(s) 127, 556
BspACI CCGC 5 cut(s) 5, 296, 335, 468, 470
BspFNI CGCG 2 cut(s) 296, 470
BspHI TCATGA 1 cut(s) 674
BspLI GGNNCC 3 cut(s) 300, 339, 598
BspMAI CTGCAG 1 cut(s) 154
BspPI GGATC 1 cut(s) 551
BsrBI CCGCTC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 508
BsrGI TGTACA 2 cut(s) 583, 622
BssECI CCNNGG 3 cut(s) 96, 264, 468
BssMI GATC 2 cut(s) 127, 556
BssT1I CCWWGG 1 cut(s) 96
Bst4CI ACNGT 3 cut(s) 85, 313, 396
BstAUI TGTACA 2 cut(s) 583, 622
BstC8I GCNNGC 2 cut(s) 446, 484
BstDSI CCRYGG 2 cut(s) 264, 468
BstF5I GGATG 1 cut(s) 213
BstFNI CGCG 2 cut(s) 296, 470
BstKTI GATC 2 cut(s) 130, 559
BstMBI GATC 2 cut(s) 127, 556
BstMWI GCNNNNNNNGC 2 cut(s) 396, 479
BstSCI CCNGG 1 cut(s) 522
BstSFI CTRYAG 2 cut(s) 150, 456
BstUI CGCG 2 cut(s) 296, 470
BstV1I GCAGC 3 cut(s) 136, 164, 421
BtgI CCRYGG 2 cut(s) 264, 468
BtsCI GGATG 1 cut(s) 213
BtsIMutI CAGTG 2 cut(s) 90, 401
Cac8I GCNNGC 2 cut(s) 446, 484
CciI TCATGA 1 cut(s) 674
Cfr13I GGNCC 1 cut(s) 476
Cfr42I CCGCGG 1 cut(s) 471
CseI GACGC 1 cut(s) 302
Csp6I GTAC 3 cut(s) 106, 584, 623
CviAII CATG 5 cut(s) 24, 65, 320, 620, 675
CviJI RGCY 9 cut(s) 18, 124, 149, 155, 244, 301, 444, 589, 599
CviKI_1 RGCY 9 cut(s) 18, 124, 149, 155, 244, 301, 444, 589, 599
CviQI GTAC 3 cut(s) 106, 584, 623
DpnI GATC 2 cut(s) 129, 558
DpnII GATC 2 cut(s) 127, 556
DraIII CACNNNGTG 1 cut(s) 85
DrdI GACNNNNNNGTC 1 cut(s) 533
DseDI GACNNNNNNGTC 1 cut(s) 533
Eco130I CCWWGG 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 476
Eco57I CTGAAG 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 5 cut(s) 27, 68, 323, 623, 678
FaqI GGGAC 2 cut(s) 299, 521
FatI CATG 5 cut(s) 23, 64, 319, 619, 674
FblI GTMKAC 1 cut(s) 660
Fnu4HI GCNGC 5 cut(s) 5, 150, 153, 410, 471
FokI GGATG 1 cut(s) 200
Fsp4HI GCNGC 5 cut(s) 5, 150, 153, 410, 471
GluI GCNGC 5 cut(s) 5, 150, 153, 410, 471
GsuI CTGGAG 1 cut(s) 615
HapII CCGG 2 cut(s) 375, 524
HgaI GACGC 1 cut(s) 302
Hin1II CATG 5 cut(s) 27, 68, 323, 623, 678
HinfI GANTC 1 cut(s) 92
HpaII CCGG 2 cut(s) 375, 524
HphI GGTGA 2 cut(s) 389, 518
Hpy166II GTNNAC 2 cut(s) 491, 661
Hpy188I TCNGA 2 cut(s) 292, 636
Hpy188III TCNNGA 3 cut(s) 418, 594, 675
Hpy8I GTNNAC 2 cut(s) 491, 661
HpyCH4III ACNGT 3 cut(s) 85, 313, 396
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 2 cut(s) 152, 399
HpyF10VI GCNNNNNNNGC 2 cut(s) 396, 479
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 5 cut(s) 27, 68, 323, 623, 678
KspI CCGCGG 1 cut(s) 471
Kzo9I GATC 2 cut(s) 127, 556
LmnI GCTCC 2 cut(s) 298, 596
LpnPI CCDG 9 cut(s) 152, 388, 459, 468, 483, 492, 537, 579, 579
Lsp1109I GCAGC 3 cut(s) 136, 164, 421
LweI GCATC 1 cut(s) 366
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 2 cut(s) 229, 427
MalI GATC 2 cut(s) 129, 558
MbiI CCGCTC 1 cut(s) 7
MboI GATC 2 cut(s) 127, 556
MluCI AATT 3 cut(s) 133, 463, 541
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 2 cut(s) 270, 570
MnlI CCTC 3 cut(s) 125, 346, 627
MseI TTAA 1 cut(s) 132
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 2 cut(s) 375, 524
MspR9I CCNGG 1 cut(s) 524
MvnI CGCG 2 cut(s) 296, 470
MwoI GCNNNNNNNGC 2 cut(s) 396, 479
NciI CCSGG 1 cut(s) 524
NdeII GATC 2 cut(s) 127, 556
NlaIII CATG 5 cut(s) 27, 68, 323, 623, 678
NlaIV GGNNCC 3 cut(s) 300, 339, 598
NmuCI GTSAC 1 cut(s) 229
PagI TCATGA 1 cut(s) 674
PflMI CCANNNNNTGG 1 cut(s) 85
PfoI TCCNGGA 1 cut(s) 522
PkrI GCNGC 5 cut(s) 6, 151, 154, 411, 472
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
PspN4I GGNNCC 3 cut(s) 300, 339, 598
PspPI GGNCC 1 cut(s) 476
PstI CTGCAG 1 cut(s) 154
RsaI GTAC 3 cut(s) 107, 585, 624
RsaNI GTAC 3 cut(s) 106, 584, 623
SacII CCGCGG 1 cut(s) 471
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 5 cut(s) 5, 150, 153, 410, 471
Sau3AI GATC 2 cut(s) 127, 556
Sau96I GGNCC 1 cut(s) 476
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 524
SetI ASST 5 cut(s) 20, 117, 224, 231, 246
SfaNI GCATC 1 cut(s) 366
SfcI CTRYAG 2 cut(s) 150, 456
Sfr303I CCGCGG 1 cut(s) 471
SgrBI CCGCGG 1 cut(s) 471
SinI GGWCC 1 cut(s) 476
Sse9I AATT 3 cut(s) 133, 463, 541
SsiI CCGC 5 cut(s) 5, 296, 335, 468, 470
StyD4I CCNGG 1 cut(s) 522
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 3 cut(s) 85, 313, 396
TaiI ACGT 1 cut(s) 231
TaqI TCGA 1 cut(s) 50
TaqII GACCGA 2 cut(s) 199, 641
TasI AATT 3 cut(s) 133, 463, 541
TatI WGTACW 2 cut(s) 583, 622
TauI GCSGC 2 cut(s) 7, 473
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TscAI CASTG 2 cut(s) 90, 401
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 3 cut(s) 149, 152, 409
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 4 cut(s) 53, 528, 594, 631
TspGWI ACGGA 1 cut(s) 450
TspRI CASTG 2 cut(s) 90, 401
Van91I CCANNNNNTGG 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.