Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3D

Orthogroup ID

OG0002309

Physical Location & Seq

Genomic Coordinates Forward (+)

18012431 .. 18016352

Gene Structure 3922 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3DG199100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2112 bp

ATGATTACGTTTATGGATTCGAAAGAAAAATTGAAGGAGGGAGAGAAGTGCTTGGATCCTCAGCTATGGCATGCCTGTGCCGGAGGGATGGTTCAAATGCCATCGGTGAATGCCAAGGTGTTTTACTTCCCTCAGGGCCACGCCGAACACGCTTGTGGCCCTGTCGATTTCAGGAACTGCCCGAGGATTCCGCCTTACATACTCTGCAGGGTCTCGGCCATCAAGTTCATGGCTGATCCTGAGACTGATGAGGTTTATGCCAAGATTAGGCTGGTTCCTTTGAGCTCCAGTGAGTCTGGTTATGAGGATAATGGAATTGGGGGGATTAATGGGGCTGATTCGCAAGATAAGCCGGCCTCATTTGCAAAGACGCTGACTCAGTCGGATGCTAACAATGGTGGGGGGTTTTCTGTGCCGAGATACTGTGCTGAAACCATCTTTCCCAGATTGGACTACTCGGCTGATCCTCCTGTGCAGACAATTCTGGCTAAGGATGTTCATGGCGAGACTTGGAAGTTTAGGCACATATATAGGGGGACGCCGAGGCGGCATCTATTGACCACAGGGTGGAGTACTTTTGTGAACCACAAGAAGCTTGTTGCTGGAGACTCGATTGTGTTTTTGAGGGCGGAGAACGGTGATCTTTGTGTTGGAATTCGGCGAGCCAAGAGGGGAATTGGGGGTGGGCCGGAGTCATCTTCTGGGTGGAATCCGGCGGGTGGGAATTGTGCCATGCCATATGGAGGGTTCTCTTCCTATTTGAGGGAGGATGAGGGGAAACTGATGAGAAATGGCAATGGTAATGGCTCGAATTCGAATGGAAGTCTAATGGGGAAGGGGAAAGTTGGTCCTGAATCAGTTTTGGAAGCTGCAACACTTGCTTCAAATGGACAACCCTTTGAGGTTGTTTACTACCCTCGAGCAAGTACTCCAGAGTTTTGTGTGAAAGCCTCGTTGGTGAAGGCAGCACTGCAGATCCGTTGGTCCCCCGGAATGAGATTTAAGATGGCCTTTGAAACCGAAGATTCTTCCAGGATTAGTTGGTTCATGGGAACCATATCCTCTGTTCATGTTTCTGAGCCTATGCGCTGGCCTGAATCACCTTGGCGGCTTCTCCAGGTTAGCTGGGATGAACCTGATTTGCTTCAGAATGTTAAGCGCGTTAGCCCGTGGCTGGTAGAATTGGTGTCAAACATGCCCGCCATCCATCTGACACCCTTCTCACCACCAAGGAAGAAGATGAGACTGCCACAACACCCCGATTTCCCATTTGAGGGACAACTTCCAATGCCGACATTTTCCGGCAATCACCACCTCCTTGGAACCAGCAGCCCCTTCGGTTGTCTGCCCGACAAAACTCCTGCTGGCATGCAGGGAGCCAGGCATGCTCATTACGGTCTATCCTTATCAGATATCCACCTCAATAAACTGCAATCAGGTCTATTTCCGGCTGGTTTCCCGCCACTTGATCATGTTGCTACTCCCACCAAATTCTCCAGTAACACAATGATTCAAAGGCCTACCATGAGCGAGAATGTGTCTTGCTTGTTAACTATGGCACATTCTCCACAGTCTTCAAAGAAACCCGATGATGTAAAGCCACCGCAGCTCATGCTCTTCGGTCAACCAATACTTACTGAGCAGCAAATCTCTATGAGCTCCTCTGGCGATACTGTCTCACCAGTGCTTACTGGAAATAGTTCGTCAGATGGAAGTGGAGATAAAATGGCAAACCATTCTGATAATTCTGGATCTGCACTTCACCAACAAAGTGTACAAGAGCGATCATCCGGTGAAGGTTTCCATTGGTATAAGGACACTCGGCATGAAGCGGAACCAAATTTGGAAACTGGTCACTGTAAAGTCTTTATGGAATCTGAAGATGTGGGCCGCACTCTTGACTTTTCACAGTTCAAGTCTTATGATGAATTGTATACGAAACTGGCAGACATGTTTGGCATAGAAAATTCCGAGACACTGAACCATGTGCTCTATCGAGATGCTACCGGTGCAGTCAAACACATTGGAGACGAACTATTCAGTGACTTTGTGAAAACGGCAAGGAGATTGACGATTCTAATGGATTCAGGCAGCAACAATGTAGGAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Auxin_resp B3

Protein Analysis

703

Amino Acids

77.24

Weight (kDa)

6.48

Isoelectric Point (pI)

53.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B3	PF02362	121 - 222	3.5e-23	B3 DNA binding domain
BRWD_AD	PF25313	295 - 396	2.3e-09	BRWD/PHIP ancillary domain-like domain
ARF_AD	PF06507	313 - 396	3.4e-33	Auxin response factor ancillary domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002309)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28350 AT2G28350
fragaria_vesca	FvH4_3g32000 FvH4_3g32000 FvH4_6g15960 FvH4_6g15960 FvH4_6g15960
malus_domestica	MD03G1119500.v1.1 MD03G1119700.v1.1 MD04G1096900.v1.1
prunus_persica	Prupe.6G102800_v2.0.a1 Prupe.6G102800_v2.0.a1 Prupe.6G102800_v2.0.a1 Prupe.6G236200_v2.0.a1 Prupe.6G236200_v2.0.a1 Prupe.6G236200_v2.0.a1
pyrus_communis	pycom04g09200 pycom11g11370 pycom12g11700
rosa_chinensis	RchiOBHm_Chr3g0469661 RchiOBHm_Chr5g0058761
rosa_laevigata	RLG00000024330 RLG00000035245
rosa_multiflora	Rmu_sc0000367.1_g000238 Rmu_sc0006483.1_g000023 Rmu_sc0030116.1_g000001
rosa_roxburghii	Rroxscaffold_1G00021650 Rroxscaffold_6G00411580
rosa_rugosa	Rorug03G0103100 Rorug03G0103200 Rorug03G0103300 Rorug05G0317900
rosa_samantha	Rh3AG152700 Rh3BG176500 Rh3CG167500 Rh3DG199100 Rh5AG383600 Rh5BG395500 Rh5CG419200 Rh5DG410900
rosa_wichuraiana	Rw3G014400 Rw5G036140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	567
AccI	GTMKAC	1 cut(s)	1934
AccII	CGCG	1 cut(s)	1161
AclWI	GGATC	6 cut(s)	50, 63, 230, 458, 970, 1759
AcoI	YGGCCR	1 cut(s)	216
AcsI	RAATTY	5 cut(s)	654, 811, 1490, 1840, 1966
AcuI	CTGAAG	2 cut(s)	1130, 1899
AcyI	GRCGYC	1 cut(s)	539
AdeI	CACNNNGTG	1 cut(s)	567
AfaI	GTAC	3 cut(s)	574, 928, 1776
AfiI	CCNNNNNNNGG	8 cut(s)	267, 567, 719, 743, 762, 1174, 1273, 1274
AflIII	ACRYGT	1 cut(s)	1950
AgeI	ACCGGT	1 cut(s)	2006
AgsI	TTSAA	7 cut(s)	34, 95, 885, 1016, 1514, 1578, 1915
AjnI	CCWGG	3 cut(s)	1031, 1116, 1379
AjuI	GAANNNNNNNTTGG	2 cut(s)	938, 970
AleI	CACNNNNGTG	1 cut(s)	153
AloI	GAACNNNNNNTCC	2 cut(s)	75, 107
AluBI	AGCT	7 cut(s)	64, 285, 595, 869, 1125, 1609, 1659
AluI	AGCT	7 cut(s)	64, 285, 595, 869, 1125, 1609, 1659
Alw21I	GWGCWC	3 cut(s)	287, 1661, 1992
Alw26I	GTCTC	8 cut(s)	217, 236, 500, 600, 1237, 1681, 1967, 2022
AlwI	GGATC	6 cut(s)	50, 63, 230, 458, 970, 1759
AlwNI	CAGNNNCTG	1 cut(s)	177
Ama87I	CYCGRG	2 cut(s)	181, 918
AoxI	GGCC	9 cut(s)	136, 157, 216, 354, 686, 1008, 1091, 1517, 1888
ApeKI	GCWGC	6 cut(s)	869, 965, 1329, 1606, 1642, 2091
ApoI	RAATTY	5 cut(s)	654, 811, 1490, 1840, 1966
AseI	ATTAAT	1 cut(s)	327
AsiGI	ACCGGT	1 cut(s)	2006
Asp700I	GAANNNNTTC	1 cut(s)	1699
AspLEI	GCGC	2 cut(s)	1089, 1161
AspS9I	GGNCC	6 cut(s)	136, 158, 686, 848, 984, 1888
AsuC2I	CCSGG	1 cut(s)	990
AsuHPI	GGTGA	9 cut(s)	118, 650, 970, 1092, 1215, 1301, 1671, 1754, 1805
AsuII	TTCGAA	2 cut(s)	20, 815
AvaI	CYCGRG	2 cut(s)	181, 918
AvaII	GGWCC	2 cut(s)	848, 984
AxyI	CCTNAGG	1 cut(s)	132
BamHI	GGATCC	1 cut(s)	55
BanII	GRGCYC	2 cut(s)	287, 1661
BbsI	GAAGAC	1 cut(s)	1566
Bbv12I	GWGCWC	3 cut(s)	287, 1661, 1992
BbvCI	CCTCAGC	1 cut(s)	60
BbvI	GCAGC	6 cut(s)	856, 977, 1341, 1618, 1654, 2103
BccI	CCATC	8 cut(s)	82, 109, 227, 443, 1000, 1211, 1215, 1703
BceAI	ACGGC	1 cut(s)	2073
BcgI	CGANNNNNNTGC	2 cut(s)	1318, 1352
BciT130I	CCWGG	3 cut(s)	1033, 1118, 1381
BclI	TGATCA	1 cut(s)	1468
BcnI	CCSGG	1 cut(s)	990
BcoDI	GTCTC	8 cut(s)	217, 236, 500, 600, 1237, 1681, 1967, 2022
BfmI	CTRYAG	2 cut(s)	205, 971
BglI	GCCNNNNNGGC	1 cut(s)	547
BisI	GCNGC	9 cut(s)	548, 870, 966, 1109, 1330, 1607, 1643, 1891, 2092
BlsI	GCNGC	9 cut(s)	549, 871, 967, 1110, 1331, 1608, 1644, 1892, 2093
BmcAI	AGTACT	2 cut(s)	574, 928
Bme1390I	CCNGG	4 cut(s)	990, 1033, 1118, 1381
Bme18I	GGWCC	2 cut(s)	848, 984
BmeT110I	CYCGRG	2 cut(s)	181, 918
BmgT120I	GGNCC	6 cut(s)	136, 158, 686, 848, 984, 1888
BmiI	GGNNCC	7 cut(s)	57, 276, 986, 1054, 1324, 1378, 1836
BmrFI	CCNGG	4 cut(s)	990, 1033, 1118, 1381
BmsI	GCATC	3 cut(s)	376, 559, 1990
BpiI	GAAGAC	1 cut(s)	1566
BpmI	CTGGAG	5 cut(s)	271, 624, 915, 1100, 1480
Bpu10I	CCTNAGC	2 cut(s)	60, 489
Bpu14I	TTCGAA	2 cut(s)	20, 815
BpuMI	CCSGG	1 cut(s)	990
BsaHI	GRCGYC	1 cut(s)	539
BsaI	GGTCTC	1 cut(s)	217
BsaJI	CCNNGG	8 cut(s)	114, 182, 542, 988, 1103, 1169, 1229, 1318
BsaWI	WCCGGW	2 cut(s)	1790, 2006
BsaXI	ACNNNNNCTCC	2 cut(s)	75, 105
Bsc4I	CCNNNNNNNGG	8 cut(s)	267, 567, 719, 743, 762, 1174, 1273, 1274
Bse118I	RCCGGY	2 cut(s)	352, 2006
Bse1I	ACTGG	6 cut(s)	288, 1497, 1682, 1696, 1855, 1947
Bse21I	CCTNAGG	1 cut(s)	132
Bse3DI	GCAATG	1 cut(s)	802
BseBI	CCWGG	3 cut(s)	1033, 1118, 1381
BseDI	CCNNGG	8 cut(s)	114, 182, 542, 988, 1103, 1169, 1229, 1318
BseGI	GGATG	7 cut(s)	93, 391, 499, 775, 1135, 1203, 1787
BseLI	CCNNNNNNNGG	8 cut(s)	267, 567, 719, 743, 762, 1174, 1273, 1274
BseMI	GCAATG	1 cut(s)	802
BseMII	CTCAG	6 cut(s)	74, 146, 231, 392, 1068, 1629
BseNI	ACTGG	6 cut(s)	288, 1497, 1682, 1696, 1855, 1947
BseRI	GAGGAG	1 cut(s)	1651
BseXI	GCAGC	6 cut(s)	856, 977, 1341, 1618, 1654, 2103
BseYI	CCCAGC	1 cut(s)	1125
BsgI	GTGCAG	3 cut(s)	494, 1740, 2031
Bsh1236I	CGCG	1 cut(s)	1161
BshFI	GGCC	9 cut(s)	138, 159, 218, 356, 688, 1010, 1093, 1519, 1890
BshTI	ACCGGT	1 cut(s)	2006
BsiHKAI	GWGCWC	3 cut(s)	287, 1661, 1992
BsiHKCI	CYCGRG	2 cut(s)	181, 918
BsiSI	CCGG	9 cut(s)	81, 353, 689, 713, 990, 1302, 1448, 1791, 2007
BslFI	GGGAC	3 cut(s)	550, 970, 1290
BslI	CCNNNNNNNGG	8 cut(s)	267, 567, 719, 743, 762, 1174, 1273, 1274
BsmAI	GTCTC	8 cut(s)	217, 236, 500, 600, 1237, 1681, 1967, 2022
BsmBI	CGTCTC	1 cut(s)	2022
BsmFI	GGGAC	3 cut(s)	550, 970, 1290
BsmI	GAATGC	1 cut(s)	115
BsnI	GGCC	9 cut(s)	138, 159, 218, 356, 688, 1010, 1093, 1519, 1890
Bso31I	GGTCTC	1 cut(s)	217
BsoBI	CYCGRG	2 cut(s)	181, 918
Bsp119I	TTCGAA	2 cut(s)	20, 815
Bsp1286I	GDGCHC	3 cut(s)	287, 1661, 1992
Bsp1407I	TGTACA	1 cut(s)	1774
Bsp143I	GATC	8 cut(s)	55, 235, 463, 640, 975, 1468, 1751, 1784
BspANI	GGCC	9 cut(s)	138, 159, 218, 356, 688, 1010, 1093, 1519, 1890
BspCNI	CTCAG	6 cut(s)	73, 145, 232, 391, 1069, 1630
BspFNI	CGCG	1 cut(s)	1161
BspLI	GGNNCC	7 cut(s)	57, 276, 986, 1054, 1324, 1378, 1836
BspMAI	CTGCAG	2 cut(s)	209, 975
BspPI	GGATC	6 cut(s)	50, 63, 230, 458, 970, 1759
BspQI	GCTCTTC	1 cut(s)	1622
BspT104I	TTCGAA	2 cut(s)	20, 815
BspTNI	GGTCTC	1 cut(s)	217
BsrDI	GCAATG	1 cut(s)	802
BsrFI	RCCGGY	2 cut(s)	352, 2006
BsrGI	TGTACA	1 cut(s)	1774
BsrI	ACTGG	6 cut(s)	288, 1497, 1682, 1696, 1855, 1947
BssAI	RCCGGY	2 cut(s)	352, 2006
BssECI	CCNNGG	8 cut(s)	114, 182, 542, 988, 1103, 1169, 1229, 1318
BssMI	GATC	8 cut(s)	55, 235, 463, 640, 975, 1468, 1751, 1784
BssNAI	GTATAC	1 cut(s)	1935
BssNI	GRCGYC	1 cut(s)	539
BssT1I	CCWWGG	4 cut(s)	114, 1103, 1229, 1318
Bst1107I	GTATAC	1 cut(s)	1935
Bst2UI	CCWGG	3 cut(s)	1033, 1118, 1381
Bst4CI	ACNGT	7 cut(s)	425, 638, 1397, 1572, 1675, 1859, 1911
Bst6I	CTCTTC	2 cut(s)	757, 1622
BstACI	GRCGYC	1 cut(s)	539
BstAPI	GCANNNNNTGC	2 cut(s)	878, 1612
BstAUI	TGTACA	1 cut(s)	1774
BstBI	TTCGAA	2 cut(s)	20, 815
BstC8I	GCNNGC	8 cut(s)	72, 354, 663, 1091, 1200, 1366, 1370, 1386
BstDEI	CTNAG	7 cut(s)	60, 132, 240, 378, 489, 1077, 1638
BstDSI	CCRYGG	1 cut(s)	1169
BstENI	CCTNNNNNAGG	1 cut(s)	760
BstF5I	GGATG	7 cut(s)	93, 391, 499, 775, 1135, 1203, 1787
BstFNI	CGCG	1 cut(s)	1161
BstHHI	GCGC	2 cut(s)	1089, 1161
BstKTI	GATC	8 cut(s)	58, 238, 466, 643, 978, 1471, 1754, 1787
BstMAI	GTCTC	8 cut(s)	217, 236, 500, 600, 1237, 1681, 1967, 2022
BstMBI	GATC	8 cut(s)	55, 235, 463, 640, 975, 1468, 1751, 1784
BstNI	CCWGG	3 cut(s)	1033, 1118, 1381
BstNSI	RCATGY	5 cut(s)	74, 1198, 1372, 1388, 1954
BstSCI	CCNGG	4 cut(s)	988, 1031, 1116, 1379
BstSFI	CTRYAG	2 cut(s)	205, 971
BstUI	CGCG	1 cut(s)	1161
BstV1I	GCAGC	6 cut(s)	856, 977, 1341, 1618, 1654, 2103
BstV2I	GAAGAC	1 cut(s)	1566
BstX2I	RGATCY	3 cut(s)	55, 975, 1751
BstXI	CCANNNNNNTGG	1 cut(s)	1319
BstYI	RGATCY	3 cut(s)	55, 975, 1751
BstZ17I	GTATAC	1 cut(s)	1935
Bsu36I	CCTNAGG	1 cut(s)	132
BsuRI	GGCC	9 cut(s)	138, 159, 218, 356, 688, 1010, 1093, 1519, 1890
BtgI	CCRYGG	1 cut(s)	1169
BtsCI	GGATG	7 cut(s)	93, 391, 499, 775, 1135, 1203, 1787
BtsI	GCAGTG	1 cut(s)	968
BtsIMutI	CAGTG	6 cut(s)	295, 968, 1689, 1855, 1976, 2047
Cac8I	GCNNGC	8 cut(s)	72, 354, 663, 1091, 1200, 1366, 1370, 1386
CaiI	CAGNNNCTG	1 cut(s)	177
CfoI	GCGC	2 cut(s)	1089, 1161
Cfr10I	RCCGGY	2 cut(s)	352, 2006
Cfr13I	GGNCC	6 cut(s)	136, 158, 686, 848, 984, 1888
CseI	GACGC	2 cut(s)	379, 547
Csp6I	GTAC	3 cut(s)	573, 927, 1775
CspAI	ACCGGT	1 cut(s)	2006
CviQI	GTAC	3 cut(s)	573, 927, 1775
DdeI	CTNAG	7 cut(s)	60, 132, 240, 378, 489, 1077, 1638
DpnI	GATC	8 cut(s)	57, 237, 465, 642, 977, 1470, 1753, 1786
DpnII	GATC	8 cut(s)	55, 235, 463, 640, 975, 1468, 1751, 1784
DraIII	CACNNNGTG	1 cut(s)	567
EaeI	YGGCCR	1 cut(s)	216
Eam1104I	CTCTTC	2 cut(s)	757, 1622
EarI	CTCTTC	2 cut(s)	757, 1622
EciI	GGCGGA	2 cut(s)	180, 644
Ecl136II	GAGCTC	2 cut(s)	285, 1659
Eco130I	CCWWGG	4 cut(s)	114, 1103, 1229, 1318
Eco147I	AGGCCT	1 cut(s)	1519
Eco24I	GRGCYC	2 cut(s)	287, 1661
Eco31I	GGTCTC	1 cut(s)	217
Eco32I	GATATC	1 cut(s)	1414
Eco47I	GGWCC	2 cut(s)	848, 984
Eco53kI	GAGCTC	2 cut(s)	285, 1659
Eco57I	CTGAAG	2 cut(s)	1130, 1899
Eco81I	CCTNAGG	1 cut(s)	132
Eco88I	CYCGRG	2 cut(s)	181, 918
EcoICRI	GAGCTC	2 cut(s)	285, 1659
EcoNI	CCTNNNNNAGG	1 cut(s)	760
EcoRI	GAATTC	2 cut(s)	654, 811
EcoRII	CCWGG	3 cut(s)	1031, 1116, 1379
EcoRV	GATATC	1 cut(s)	1414
EcoT14I	CCWWGG	4 cut(s)	114, 1103, 1229, 1318
EcoT38I	GRGCYC	2 cut(s)	287, 1661
ErhI	CCWWGG	4 cut(s)	114, 1103, 1229, 1318
Esp3I	CGTCTC	1 cut(s)	2022
FalI	AAGNNNNNCTT	2 cut(s)	995, 1027
FaqI	GGGAC	3 cut(s)	550, 970, 1290
FauI	CCCGC	3 cut(s)	709, 1207, 1467
FauNDI	CATATG	1 cut(s)	739
FbaI	TGATCA	1 cut(s)	1468
FblI	GTMKAC	1 cut(s)	1934
Fnu4HI	GCNGC	9 cut(s)	548, 870, 966, 1109, 1330, 1607, 1643, 1891, 2092
FokI	GGATG	7 cut(s)	100, 398, 506, 782, 1142, 1190, 1774
FriOI	GRGCYC	2 cut(s)	287, 1661
Fsp4HI	GCNGC	9 cut(s)	548, 870, 966, 1109, 1330, 1607, 1643, 1891, 2092
GlaI	GCGC	2 cut(s)	1088, 1160
GluI	GCNGC	9 cut(s)	548, 870, 966, 1109, 1330, 1607, 1643, 1891, 2092
GsaI	CCCAGC	1 cut(s)	1129
GsuI	CTGGAG	5 cut(s)	271, 624, 915, 1100, 1480
HaeIII	GGCC	9 cut(s)	138, 159, 218, 356, 688, 1010, 1093, 1519, 1890
HapII	CCGG	9 cut(s)	81, 353, 689, 713, 990, 1302, 1448, 1791, 2007
HgaI	GACGC	2 cut(s)	379, 547
HhaI	GCGC	2 cut(s)	1089, 1161
Hin1I	GRCGYC	1 cut(s)	539
Hin6I	GCGC	2 cut(s)	1087, 1159
HinP1I	GCGC	2 cut(s)	1087, 1159
HincII	GTYRAC	2 cut(s)	1551, 1625
HindII	GTYRAC	2 cut(s)	1551, 1625
HindIII	AAGCTT	1 cut(s)	593
HpaI	GTTAAC	1 cut(s)	1551
HpaII	CCGG	9 cut(s)	81, 353, 689, 713, 990, 1302, 1448, 1791, 2007
HphI	GGTGA	9 cut(s)	118, 650, 970, 1092, 1215, 1301, 1671, 1754, 1805
Hpy166II	GTNNAC	6 cut(s)	583, 910, 1551, 1625, 1775, 1935
Hpy188I	TCNGA	9 cut(s)	385, 1078, 1149, 1212, 1411, 1708, 1741, 1879, 1972
Hpy188III	TCNNGA	7 cut(s)	172, 239, 851, 932, 1749, 1898, 1997
Hpy8I	GTNNAC	6 cut(s)	583, 910, 1551, 1625, 1775, 1935
HpyAV	CCTTC	6 cut(s)	28, 829, 955, 1228, 1345, 1790
HpyCH4III	ACNGT	7 cut(s)	425, 638, 1397, 1572, 1675, 1859, 1911
HpyCH4IV	ACGT	1 cut(s)	8
HpyCH4V	TGCA	9 cut(s)	207, 365, 475, 872, 973, 1372, 1432, 1757, 2012
HpyF3I	CTNAG	7 cut(s)	60, 132, 240, 378, 489, 1077, 1638
HpySE526I	ACGT	1 cut(s)	8
Hsp92I	GRCGYC	1 cut(s)	539
HspAI	GCGC	2 cut(s)	1087, 1159
KroI	GCCGGC	1 cut(s)	352
KroNI	GCCGGC	1 cut(s)	354
Ksp22I	TGATCA	1 cut(s)	1468
KspAI	GTTAAC	1 cut(s)	1551
Kzo9I	GATC	8 cut(s)	55, 235, 463, 640, 975, 1468, 1751, 1784
LguI	GCTCTTC	1 cut(s)	1622
LmnI	GCTCC	3 cut(s)	290, 1376, 1664
Lsp1109I	GCAGC	6 cut(s)	856, 977, 1341, 1618, 1654, 2103
LweI	GCATC	3 cut(s)	376, 559, 1990
MaeII	ACGT	1 cut(s)	8
MaeIII	GTNAC	3 cut(s)	1499, 1853, 2042
MalI	GATC	8 cut(s)	57, 237, 465, 642, 977, 1470, 1753, 1786
MboI	GATC	8 cut(s)	55, 235, 463, 640, 975, 1468, 1751, 1784
MboII	GAAGA	9 cut(s)	690, 744, 1020, 1034, 1246, 1249, 1566, 1609, 1892
MflI	RGATCY	3 cut(s)	55, 975, 1751
MhlI	GDGCHC	3 cut(s)	287, 1661, 1992
MlyI	GAGTC	4 cut(s)	302, 370, 602, 701
MmeI	TCCRAC	2 cut(s)	363, 631
MroNI	GCCGGC	1 cut(s)	352
MroXI	GAANNNNTTC	1 cut(s)	1699
MseI	TTAA	4 cut(s)	327, 1002, 1155, 1550
MslI	CAYNNNNRTG	2 cut(s)	75, 153
MspI	CCGG	9 cut(s)	81, 353, 689, 713, 990, 1302, 1448, 1791, 2007
MspR9I	CCNGG	4 cut(s)	990, 1033, 1118, 1381
Mva1269I	GAATGC	1 cut(s)	115
MvaI	CCWGG	3 cut(s)	1033, 1118, 1381
MvnI	CGCG	1 cut(s)	1161
NaeI	GCCGGC	1 cut(s)	354
NciI	CCSGG	1 cut(s)	990
NdeI	CATATG	1 cut(s)	739
NdeII	GATC	8 cut(s)	55, 235, 463, 640, 975, 1468, 1751, 1784
NgoMIV	GCCGGC	1 cut(s)	352
NlaIV	GGNNCC	7 cut(s)	57, 276, 986, 1054, 1324, 1378, 1836
NmeAIII	GCCGAG	5 cut(s)	194, 437, 441, 567, 1801
NmuCI	GTSAC	2 cut(s)	1853, 2042
NspI	RCATGY	5 cut(s)	74, 1198, 1372, 1388, 1954
NspV	TTCGAA	2 cut(s)	20, 815
OliI	CACNNNNGTG	1 cut(s)	153
PaeI	GCATGC	3 cut(s)	74, 1372, 1388
PaeR7I	CTCGAG	1 cut(s)	918
PceI	AGGCCT	1 cut(s)	1519
PciI	ACATGT	1 cut(s)	1950
PciSI	GCTCTTC	1 cut(s)	1622
PctI	GAATGC	1 cut(s)	115
PdiI	GCCGGC	1 cut(s)	354
PdmI	GAANNNNTTC	1 cut(s)	1699
PflFI	GACNNNGTC	1 cut(s)	379
PflMI	CCANNNNNTGG	1 cut(s)	567
PfoI	TCCNGGA	1 cut(s)	1031
PinAI	ACCGGT	1 cut(s)	2006
PkrI	GCNGC	9 cut(s)	549, 871, 967, 1110, 1331, 1608, 1644, 1892, 2093
PleI	GAGTC	4 cut(s)	301, 370, 602, 700
PpsI	GAGTC	4 cut(s)	301, 370, 602, 700
PscI	ACATGT	1 cut(s)	1950
PshBI	ATTAAT	1 cut(s)	327
Psp124BI	GAGCTC	2 cut(s)	287, 1661
Psp6I	CCWGG	3 cut(s)	1031, 1116, 1379
PspFI	CCCAGC	1 cut(s)	1125
PspGI	CCWGG	3 cut(s)	1031, 1116, 1379
PspN4I	GGNNCC	7 cut(s)	57, 276, 986, 1054, 1324, 1378, 1836
PspPI	GGNCC	6 cut(s)	136, 158, 686, 848, 984, 1888
PspXI	VCTCGAGB	1 cut(s)	918
PstI	CTGCAG	2 cut(s)	209, 975
PstNI	CAGNNNCTG	1 cut(s)	177
PsuI	RGATCY	3 cut(s)	55, 975, 1751
PsyI	GACNNNGTC	1 cut(s)	379
RsaI	GTAC	3 cut(s)	574, 928, 1776
RsaNI	GTAC	3 cut(s)	573, 927, 1775
RseI	CAYNNNNRTG	2 cut(s)	75, 153
SacI	GAGCTC	2 cut(s)	287, 1661
SapI	GCTCTTC	1 cut(s)	1622
SaqAI	TTAA	4 cut(s)	327, 1002, 1155, 1550
SatI	GCNGC	9 cut(s)	548, 870, 966, 1109, 1330, 1607, 1643, 1891, 2092
Sau3AI	GATC	8 cut(s)	55, 235, 463, 640, 975, 1468, 1751, 1784
Sau96I	GGNCC	6 cut(s)	136, 158, 686, 848, 984, 1888
ScaI	AGTACT	2 cut(s)	574, 928
SchI	GAGTC	4 cut(s)	302, 370, 602, 701
ScrFI	CCNGG	4 cut(s)	990, 1033, 1118, 1381
SduI	GDGCHC	3 cut(s)	287, 1661, 1992
SfaNI	GCATC	3 cut(s)	376, 559, 1990
SfcI	CTRYAG	2 cut(s)	205, 971
Sfr274I	CTCGAG	1 cut(s)	918
SfuI	TTCGAA	2 cut(s)	20, 815
SinI	GGWCC	2 cut(s)	848, 984
SlaI	CTCGAG	1 cut(s)	918
SmiMI	CAYNNNNRTG	2 cut(s)	75, 153
SmlI	CTYRAG	1 cut(s)	918
SmoI	CTYRAG	1 cut(s)	918
SphI	GCATGC	3 cut(s)	74, 1372, 1388
SseBI	AGGCCT	1 cut(s)	1519
SstI	GAGCTC	2 cut(s)	287, 1661
StuI	AGGCCT	1 cut(s)	1519
StyD4I	CCNGG	4 cut(s)	988, 1031, 1116, 1379
StyI	CCWWGG	4 cut(s)	114, 1103, 1229, 1318
TaaI	ACNGT	7 cut(s)	425, 638, 1397, 1572, 1675, 1859, 1911
TaiI	ACGT	1 cut(s)	11
TaqI	TCGA	7 cut(s)	20, 165, 611, 809, 815, 919, 1996
TaqII	GACCGA	1 cut(s)	1610
TatI	WGTACW	3 cut(s)	572, 926, 1774
TauI	GCSGC	3 cut(s)	550, 1111, 1893
Tru1I	TTAA	4 cut(s)	327, 1002, 1155, 1550
Tru9I	TTAA	4 cut(s)	327, 1002, 1155, 1550
TscAI	CASTG	6 cut(s)	295, 975, 1689, 1862, 1983, 2047
TseFI	GTSAC	2 cut(s)	1853, 2042
TseI	GCWGC	6 cut(s)	869, 965, 1329, 1606, 1642, 2091
Tsp45I	GTSAC	2 cut(s)	1853, 2042
TspDTI	ATGAA	7 cut(s)	217, 488, 1036, 1058, 1146, 1842, 1941
TspGWI	ACGGA	1 cut(s)	968
TspRI	CASTG	6 cut(s)	295, 975, 1689, 1862, 1983, 2047
Tth111I	GACNNNGTC	1 cut(s)	379
Van91I	CCANNNNNTGG	1 cut(s)	567
VpaK11BI	GGWCC	2 cut(s)	848, 984
VspI	ATTAAT	1 cut(s)	327
XagI	CCTNNNNNAGG	1 cut(s)	760
XapI	RAATTY	5 cut(s)	654, 811, 1490, 1840, 1966
XceI	RCATGY	5 cut(s)	74, 1198, 1372, 1388, 1954
XcmI	CCANNNNNNNNNTGG	2 cut(s)	226, 268
XhoI	CTCGAG	1 cut(s)	918
XmiI	GTMKAC	1 cut(s)	1934
XmnI	GAANNNNTTC	1 cut(s)	1699
ZrmI	AGTACT	2 cut(s)	574, 928

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.