Rorug06G0364500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003196
Physical Location & Seq
Reverse (-)
51193537 .. 51195208
1672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0364500.1

Sequence Viewer

Length: 555 bp
ATGAACGACCCGACCCGACCCGACCCGGGAAATGAAAGTAAGAAGAAGAGGGGTCTCGGGCTTCAAGTCTTAGTCTTTTTTTTTTCGAGTGAGGAGCTGAAGGCTTGGTCTTCTCGGCGTGTCTCCGATCAGATTAGTCATTATAATGCTATACATATTGGTGGTCAGTTGTCGAAAAGAGTATCATATATATATATTGATGTTGAAAAAGTTAACAAACTTTTCTGGACCTCGGAGATCACAGATGTTCATTGTGCGTTGGGCACTTGGGTATTCTCAGTAGCAGGGGCTTTGCTAGCAATTCCGGTGGGGATAAAAAGGAAATCTTTAGGGCCACTTGTCTTCTTTGGCACAACCGGTACCATGCTTGACATCATTATGGGTATCACTCAGTGTGAAAGAGAACATGCAGAACGCCAAGCGAAGCTTTCAGAAGCCCAAAATTCTGCAACCAATGCTTCTTTTTCTGAGACTGGAGCCGAGTTGTCTTCTTTGTCTGAGACCAGAGCCGAGTATTCTTCTTTTTCCGAAACTGGATCCGAGTCTTCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

184

Amino Acids

20.2

Weight (kDa)

6.1

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 144
Acc65I GGTACC 1 cut(s) 359
AccB1I GGYRCC 1 cut(s) 359
AclWI GGATC 2 cut(s) 531, 544
AcsI RAATTY 1 cut(s) 442
AcuI CTGAAG 1 cut(s) 119
AdeI CACNNNGTG 1 cut(s) 393
AfaI GTAC 1 cut(s) 361
AfiI CCNNNNNNNGG 1 cut(s) 26
AgeI ACCGGT 1 cut(s) 356
AgsI TTSAA 3 cut(s) 65, 206, 549
AluBI AGCT 2 cut(s) 97, 427
AluI AGCT 2 cut(s) 97, 427
Alw26I GTCTC 4 cut(s) 59, 127, 464, 494
AlwI GGATC 2 cut(s) 531, 544
Ama87I CYCGRG 2 cut(s) 25, 56
AoxI GGCC 1 cut(s) 332
ApoI RAATTY 1 cut(s) 442
AsiGI ACCGGT 1 cut(s) 356
Asp718I GGTACC 1 cut(s) 359
AspS9I GGNCC 2 cut(s) 228, 332
AsuC2I CCSGG 2 cut(s) 26, 27
AsuNHI GCTAGC 1 cut(s) 295
AvaI CYCGRG 2 cut(s) 25, 56
AvaII GGWCC 1 cut(s) 228
BaeGI GKGCMC 1 cut(s) 266
BamHI GGATCC 1 cut(s) 536
BanI GGYRCC 1 cut(s) 359
BbsI GAAGAC 4 cut(s) 102, 334, 480, 537
BcnI CCSGG 2 cut(s) 26, 27
BcoDI GTCTC 4 cut(s) 59, 127, 464, 494
BfaI CTAG 1 cut(s) 296
Bme1390I CCNGG 2 cut(s) 26, 27
Bme18I GGWCC 1 cut(s) 228
BmeT110I CYCGRG 2 cut(s) 25, 56
BmgT120I GGNCC 2 cut(s) 228, 332
BmiI GGNNCC 3 cut(s) 361, 478, 538
BmrFI CCNGG 2 cut(s) 26, 27
BmtI GCTAGC 1 cut(s) 299
BpiI GAAGAC 4 cut(s) 102, 334, 480, 537
BpmI CTGGAG 1 cut(s) 495
BpuMI CCSGG 2 cut(s) 26, 27
BsaI GGTCTC 2 cut(s) 59, 494
BsaJI CCNNGG 2 cut(s) 25, 231
BsaWI WCCGGW 2 cut(s) 304, 356
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse118I RCCGGY 1 cut(s) 356
Bse1I ACTGG 2 cut(s) 478, 538
BseDI CCNNGG 2 cut(s) 25, 231
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMII CTCAG 4 cut(s) 291, 404, 459, 489
BseNI ACTGG 2 cut(s) 478, 538
BseRI GAGGAG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 266
BshFI GGCC 1 cut(s) 334
BshNI GGYRCC 1 cut(s) 359
BshTI ACCGGT 1 cut(s) 356
BsiHKCI CYCGRG 2 cut(s) 25, 56
BsiSI CCGG 3 cut(s) 26, 305, 357
BslI CCNNNNNNNGG 1 cut(s) 26
BsmAI GTCTC 4 cut(s) 59, 127, 464, 494
BsnI GGCC 1 cut(s) 334
Bso31I GGTCTC 2 cut(s) 59, 494
BsoBI CYCGRG 2 cut(s) 25, 56
Bsp1286I GDGCHC 1 cut(s) 266
Bsp143I GATC 3 cut(s) 127, 237, 536
BspANI GGCC 1 cut(s) 334
BspCNI CTCAG 4 cut(s) 290, 403, 460, 490
BspLI GGNNCC 3 cut(s) 361, 478, 538
BspOI GCTAGC 1 cut(s) 299
BspPI GGATC 2 cut(s) 531, 544
BspT107I GGYRCC 1 cut(s) 359
BspTNI GGTCTC 2 cut(s) 59, 494
BsrFI RCCGGY 1 cut(s) 356
BsrI ACTGG 2 cut(s) 478, 538
BssAI RCCGGY 1 cut(s) 356
BssECI CCNNGG 2 cut(s) 25, 231
BssMI GATC 3 cut(s) 127, 237, 536
Bst6I CTCTTC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 455
BstC8I GCNNGC 1 cut(s) 297
BstDEI CTNAG 5 cut(s) 70, 277, 390, 468, 498
BstKTI GATC 3 cut(s) 130, 240, 539
BstMAI GTCTC 4 cut(s) 59, 127, 464, 494
BstMBI GATC 3 cut(s) 127, 237, 536
BstMWI GCNNNNNNNGC 2 cut(s) 296, 455
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 2 cut(s) 24, 25
BstSLI GKGCMC 1 cut(s) 266
BstV2I GAAGAC 4 cut(s) 102, 334, 480, 537
BstX2I RGATCY 1 cut(s) 536
BstYI RGATCY 1 cut(s) 536
BsuRI GGCC 1 cut(s) 334
BtsIMutI CAGTG 1 cut(s) 398
Cac8I GCNNGC 1 cut(s) 297
Cfr10I RCCGGY 1 cut(s) 356
Cfr13I GGNCC 2 cut(s) 228, 332
Cfr9I CCCGGG 1 cut(s) 25
Csp6I GTAC 1 cut(s) 360
CspAI ACCGGT 1 cut(s) 356
CspCI CAANNNNNGTGG 2 cut(s) 288, 323
CviAII CATG 2 cut(s) 364, 407
CviJI RGCY 9 cut(s) 61, 97, 104, 290, 334, 427, 437, 479, 509
CviKI_1 RGCY 9 cut(s) 61, 97, 104, 290, 334, 427, 437, 479, 509
CviQI GTAC 1 cut(s) 360
DdeI CTNAG 5 cut(s) 70, 277, 390, 468, 498
DpnI GATC 3 cut(s) 129, 239, 538
DpnII GATC 3 cut(s) 127, 237, 536
DraIII CACNNNGTG 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco31I GGTCTC 2 cut(s) 59, 494
Eco47I GGWCC 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 119
Eco88I CYCGRG 2 cut(s) 25, 56
FaeI CATG 2 cut(s) 367, 410
FalI AAGNNNNNCTT 4 cut(s) 310, 342, 411, 443
FatI CATG 2 cut(s) 363, 406
FspBI CTAG 1 cut(s) 296
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 1 cut(s) 334
HapII CCGG 3 cut(s) 26, 305, 357
Hin1II CATG 2 cut(s) 367, 410
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 425
HinfI GANTC 1 cut(s) 542
HpaI GTTAAC 1 cut(s) 214
HpaII CCGG 3 cut(s) 26, 305, 357
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 8 cut(s) 127, 132, 235, 433, 469, 499, 529, 541
Hpy188III TCNNGA 1 cut(s) 226
Hpy8I GTNNAC 1 cut(s) 214
HpyAV CCTTC 1 cut(s) 94
HpyCH4V TGCA 2 cut(s) 410, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 455
HpyF3I CTNAG 5 cut(s) 70, 277, 390, 468, 498
Hsp92II CATG 2 cut(s) 367, 410
KpnI GGTACC 1 cut(s) 363
KspAI GTTAAC 1 cut(s) 214
Kzo9I GATC 3 cut(s) 127, 237, 536
LmnI GCTCC 2 cut(s) 94, 476
LpnPI CCDG 8 cut(s) 39, 211, 270, 318, 370, 459, 517, 519
MaeI CTAG 1 cut(s) 296
MalI GATC 3 cut(s) 129, 239, 538
MboI GATC 3 cut(s) 127, 237, 536
MboII GAAGA 7 cut(s) 55, 58, 102, 334, 480, 510, 537
MflI RGATCY 1 cut(s) 536
MhlI GDGCHC 1 cut(s) 266
MluCI AATT 2 cut(s) 300, 442
MlyI GAGTC 1 cut(s) 551
MnlI CCTC 3 cut(s) 42, 85, 241
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 3 cut(s) 144, 159, 377
MspI CCGG 3 cut(s) 26, 305, 357
MspR9I CCNGG 2 cut(s) 26, 27
MwoI GCNNNNNNNGC 2 cut(s) 296, 455
NciI CCSGG 2 cut(s) 26, 27
NdeII GATC 3 cut(s) 127, 237, 536
NheI GCTAGC 1 cut(s) 295
NlaIII CATG 2 cut(s) 367, 410
NlaIV GGNNCC 3 cut(s) 361, 478, 538
NmeAIII GCCGAG 3 cut(s) 94, 505, 535
NspI RCATGY 1 cut(s) 410
PinAI ACCGGT 1 cut(s) 356
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
PsiI TTATAA 1 cut(s) 144
PspN4I GGNNCC 3 cut(s) 361, 478, 538
PspPI GGNCC 2 cut(s) 228, 332
PsuI RGATCY 1 cut(s) 536
RsaI GTAC 1 cut(s) 361
RsaNI GTAC 1 cut(s) 360
RseI CAYNNNNRTG 3 cut(s) 144, 159, 377
SaqAI TTAA 1 cut(s) 213
Sau3AI GATC 3 cut(s) 127, 237, 536
Sau96I GGNCC 2 cut(s) 228, 332
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 2 cut(s) 26, 27
SduI GDGCHC 1 cut(s) 266
SetI ASST 3 cut(s) 99, 233, 429
SinI GGWCC 1 cut(s) 228
SmaI CCCGGG 1 cut(s) 27
SmiMI CAYNNNNRTG 3 cut(s) 144, 159, 377
Sse9I AATT 2 cut(s) 300, 442
SspMI CTAG 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 24, 25
TaqI TCGA 2 cut(s) 86, 173
TasI AATT 2 cut(s) 300, 442
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 1 cut(s) 398
TspDTI ATGAA 3 cut(s) 17, 48, 239
TspMI CCCGGG 1 cut(s) 25
TspRI CASTG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 228
XapI RAATTY 1 cut(s) 442
XceI RCATGY 1 cut(s) 410
XmaI CCCGGG 1 cut(s) 25
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.