Rh1DG062700

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005314
Physical Location & Seq
Forward (+)
11026562 .. 11038866
12305 bp
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UTR
Exon/CDS
Intron
Rh1DG062700.1

Sequence Viewer

Length: 408 bp
ATGGAAAAATCAAGAGGGAGTTCTTTTGAGAGGAGTGCAGCGAAGGATTGCTTGAGAGGAGGGTTTGCAGCCATGCCGGTGAAGGGCCCGGCTGGCCAAGAGGCTAGGATTAGAGTAAAGTCATTGCAACTACGTAACAGCTCTTTCTCTTTGAATCAAAACTCAAAAAGGAGTCTCAAAGTTTATTCATTCATTTCTATGGCGGACGAGGAGATTGCTGATCCAAAAAAGTATCTGGAGGATTCTTGTCAACCTAAATGTGTGAAGCCTTTAATTGAATATCAGGCATGTGTCAAGAGGATTGAAGGTGATGATAGTGGTTACAAGCATTGTACTGGCCAGTACTTTGACTACATATCTTGTGTTGACAAATGTGTCGCGCCAAAGTTATTTGGAAAGCTCAAGTAA

Protein Analysis

135

Amino Acids

15.1

Weight (kDa)

9.17

Isoelectric Point (pI)

55.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UCR_hinge PF02320 74 - 135 4.2e-21 Ubiquinol-cytochrome C reductase hinge protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 374
AccII CGCG 1 cut(s) 380
AciI CCGC 1 cut(s) 203
AclWI GGATC 1 cut(s) 215
AcoI YGGCCR 2 cut(s) 94, 337
AfaI GTAC 2 cut(s) 334, 344
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 3 cut(s) 154, 278, 305
AluBI AGCT 2 cut(s) 141, 400
AluI AGCT 2 cut(s) 141, 400
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 1 cut(s) 215
AoxI GGCC 3 cut(s) 85, 94, 337
ApaI GGGCCC 1 cut(s) 89
ApeKI GCWGC 2 cut(s) 38, 68
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 2 cut(s) 85, 86
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 2 cut(s) 91, 320
BaeGI GKGCMC 1 cut(s) 89
BalI TGGCCA 2 cut(s) 96, 339
BanII GRGCYC 1 cut(s) 89
BbvI GCAGC 2 cut(s) 50, 80
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 1 cut(s) 105
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 2 cut(s) 39, 69
BlsI GCNGC 2 cut(s) 40, 70
BmcAI AGTACT 1 cut(s) 344
Bme1390I CCNGG 1 cut(s) 89
BmgT120I GGNCC 2 cut(s) 85, 86
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 89
BpmI CTGGAG 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 73, 386
BpuMI CCSGG 1 cut(s) 89
BsaAI YACGTR 1 cut(s) 134
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse118I RCCGGY 1 cut(s) 76
Bse1I ACTGG 2 cut(s) 340, 340
Bse3DI GCAATG 1 cut(s) 122
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMI GCAATG 1 cut(s) 122
BseNI ACTGG 2 cut(s) 340, 340
BseRI GAGGAG 3 cut(s) 46, 72, 224
BseSI GKGCMC 1 cut(s) 89
BseXI GCAGC 2 cut(s) 50, 80
BsgI GTGCAG 1 cut(s) 57
Bsh1236I CGCG 1 cut(s) 380
BshFI GGCC 3 cut(s) 87, 96, 339
BsiSI CCGG 2 cut(s) 77, 89
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 179
BsnI GGCC 3 cut(s) 87, 96, 339
Bsp120I GGGCCC 1 cut(s) 85
Bsp1286I GDGCHC 1 cut(s) 89
Bsp143I GATC 1 cut(s) 220
BspACI CCGC 1 cut(s) 203
BspANI GGCC 3 cut(s) 87, 96, 339
BspFNI CGCG 1 cut(s) 380
BspLI GGNNCC 1 cut(s) 87
BspPI GGATC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 76
BsrI ACTGG 2 cut(s) 340, 340
BssAI RCCGGY 1 cut(s) 76
BssMI GATC 1 cut(s) 220
BstBAI YACGTR 1 cut(s) 134
BstC8I GCNNGC 1 cut(s) 94
BstFNI CGCG 1 cut(s) 380
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 1 cut(s) 223
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 1 cut(s) 93
BstNSI RCATGY 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 87
BstSLI GKGCMC 1 cut(s) 89
BstSNI TACGTA 1 cut(s) 134
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 2 cut(s) 50, 80
BsuRI GGCC 3 cut(s) 87, 96, 339
Cac8I GCNNGC 1 cut(s) 94
CfoI GCGC 1 cut(s) 382
Cfr10I RCCGGY 1 cut(s) 76
Cfr13I GGNCC 2 cut(s) 85, 86
Csp6I GTAC 2 cut(s) 333, 343
CviAII CATG 2 cut(s) 73, 288
CviJI RGCY 9 cut(s) 71, 87, 92, 96, 104, 141, 268, 339, 400
CviKI_1 RGCY 9 cut(s) 71, 87, 92, 96, 104, 141, 268, 339, 400
CviQI GTAC 2 cut(s) 333, 343
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 2 cut(s) 94, 337
EciI GGCGGA 1 cut(s) 218
Eco105I TACGTA 1 cut(s) 134
Eco24I GRGCYC 1 cut(s) 89
EcoO109I RGGNCCY 1 cut(s) 85
EcoT38I GRGCYC 1 cut(s) 89
FaeI CATG 2 cut(s) 76, 291
FaiI YATR 4 cut(s) 74, 200, 289, 356
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FatI CATG 2 cut(s) 72, 287
Fnu4HI GCNGC 2 cut(s) 39, 69
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 2 cut(s) 39, 69
FspBI CTAG 1 cut(s) 105
GlaI GCGC 1 cut(s) 381
GluI GCNGC 2 cut(s) 39, 69
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 3 cut(s) 87, 96, 339
HapII CCGG 2 cut(s) 77, 89
HhaI GCGC 1 cut(s) 382
Hin1II CATG 2 cut(s) 76, 291
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HincII GTYRAC 2 cut(s) 251, 367
HindII GTYRAC 2 cut(s) 251, 367
HinfI GANTC 3 cut(s) 154, 172, 242
HpaII CCGG 2 cut(s) 77, 89
HphI GGTGA 2 cut(s) 91, 320
Hpy166II GTNNAC 2 cut(s) 251, 367
Hpy188III TCNNGA 3 cut(s) 12, 236, 295
Hpy8I GTNNAC 2 cut(s) 251, 367
HpyAV CCTTC 3 cut(s) 37, 76, 299
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 3 cut(s) 38, 68, 127
HpyF10VI GCNNNNNNNGC 1 cut(s) 93
HpySE526I ACGT 1 cut(s) 133
Hsp92II CATG 2 cut(s) 76, 291
HspAI GCGC 1 cut(s) 380
Kzo9I GATC 1 cut(s) 220
LpnPI CCDG 7 cut(s) 78, 90, 102, 221, 269, 321, 353
Lsp1109I GCAGC 2 cut(s) 50, 80
MaeI CTAG 1 cut(s) 105
MaeII ACGT 1 cut(s) 133
MaeIII GTNAC 2 cut(s) 134, 320
MalI GATC 1 cut(s) 222
MboI GATC 1 cut(s) 220
MhlI GDGCHC 1 cut(s) 89
MlsI TGGCCA 2 cut(s) 96, 339
MluCI AATT 1 cut(s) 273
MluNI TGGCCA 2 cut(s) 96, 339
MlyI GAGTC 1 cut(s) 181
MnlI CCTC 8 cut(s) 8, 24, 50, 53, 94, 202, 232, 291
Mox20I TGGCCA 2 cut(s) 96, 339
MscI TGGCCA 2 cut(s) 96, 339
MseI TTAA 1 cut(s) 272
MslI CAYNNNNRTG 2 cut(s) 77, 197
Msp20I TGGCCA 2 cut(s) 96, 339
MspI CCGG 2 cut(s) 77, 89
MspR9I CCNGG 1 cut(s) 89
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 1 cut(s) 93
NciI CCSGG 1 cut(s) 89
NdeII GATC 1 cut(s) 220
NlaIII CATG 2 cut(s) 76, 291
NlaIV GGNNCC 1 cut(s) 87
NspI RCATGY 1 cut(s) 291
PfeI GAWTC 2 cut(s) 154, 242
PkrI GCNGC 2 cut(s) 40, 70
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
Ppu21I YACGTR 1 cut(s) 134
PspN4I GGNNCC 1 cut(s) 87
PspOMI GGGCCC 1 cut(s) 85
PspPI GGNCC 2 cut(s) 85, 86
RsaI GTAC 2 cut(s) 334, 344
RsaNI GTAC 2 cut(s) 333, 343
RseI CAYNNNNRTG 2 cut(s) 77, 197
SaqAI TTAA 1 cut(s) 272
SatI GCNGC 2 cut(s) 39, 69
Sau3AI GATC 1 cut(s) 220
Sau96I GGNCC 2 cut(s) 85, 86
ScaI AGTACT 1 cut(s) 344
SchI GAGTC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 89
SetI ASST 5 cut(s) 136, 143, 256, 310, 402
SfiI GGCCNNNNNGGCC 1 cut(s) 93
SmiMI CAYNNNNRTG 2 cut(s) 77, 197
SmlI CTYRAG 2 cut(s) 52, 401
SmoI CTYRAG 2 cut(s) 52, 401
SnaBI TACGTA 1 cut(s) 134
Sse9I AATT 1 cut(s) 273
SsiI CCGC 1 cut(s) 203
SspMI CTAG 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 87
TaiI ACGT 1 cut(s) 136
TasI AATT 1 cut(s) 273
TatI WGTACW 2 cut(s) 332, 342
TfiI GAWTC 2 cut(s) 154, 242
Tru1I TTAA 1 cut(s) 272
Tru9I TTAA 1 cut(s) 272
TseI GCWGC 2 cut(s) 38, 68
TspDTI ATGAA 2 cut(s) 177, 181
XceI RCATGY 1 cut(s) 291
XspI CTAG 1 cut(s) 105
ZrmI AGTACT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.