Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0001736

Physical Location & Seq

Genomic Coordinates Reverse (-)

30618184 .. 30625883

Gene Structure 7700 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG280500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2490 bp

ATGGTTCGAGTCTCAATTCTTGTAACATGTGTCATCTCTCTAATGTTTGTGGTCTTGACAGACCATGCTCAAGGTTTAGGTGTCAACTGGGGAGCAATGGCATCGCACCCTATGGATCCCAGCAATGTAGTAAAGATGCTCAAGGACAATGGGATCAAGAAAGTGAAGCTTTTCGACGCAGAGCCTGCCACCATGAAGGCCTTGTCCGGGTCAGGTATTTCGGTCATTGTTGGAATTCCCAATGACAAGTTGGCCTCAATTTCTTCAGCTTACAAACATGCCCAAAAATGGGTGAAAGAAAACATCACCAAATATGATCATAATGGGGGCGTTGATATCAGCTATGTGGCTGTTGGAAACGAGCCATTCTTAACAAGTTACAATGGGAGTTTTTACAAGACTACTTTTCCAGCATTGCAAAACATTCAAAAGGCTCTAGATGAAGCTGGCACCAGACAGAAAATCAAAGCCATAGTGCCCTTCAATGCCGATGTTTACGAATCTGCTTCGGGCAAACCATCGGAAGGTCAGTTCCGAAAAGACATCAAGAGTGAGATGCTCAAGATACTAGGTTTGCTCAAGAGCAATAAGTCTCCATTTTTCGTTAACATATATCCCTTCCTTAGTCTCCAACAAAGCTCTCATTTCCCCAAAGAATTTGCATTCTTTGATGGTACTAGCAAACCTGTTCGAGACAACAACCACGAATATACCAATGTGTTTGATGCAAATCATGACACACTTATTTGGTCACTCAAGAAGGTTGGTTACCAGGACTTGGACATAGTTATTGGAGAAGCGGGATGGCCTACCGATGGTGACAAGAATGCTAATAAAATTTTGGCGAAGAAATTCTATGATGGACTTCTCAAGAAATTAGCAAAGGCGGACGGAACCCCTCTTCGAAAAGGGTACTTGGAAGTGTACCTTTTTGGGCTCACTGACGAGGACAAAAAAAGTATTGACCCAGGGGACTTTGAGCGCCACTGGGGCATCTTAGGCTATGATGGAAAACCCAAGTTCCCAATGGACCTCACCGGGAAAGGGAACAATAAAATGCTCAAGGCTGTACAAGGAGTGGAGTACATGGAGAAGCAATGGTGTGTCTTGAACAGCGATGTTCGAAATGGCAGTATTTTACAAACCGAACTCAATTACGCTTGTTCTCAGACAGATTGCACATCTTTAGGAGGTGGAAGGTCATGCGATTTGGATTTGCAAGGGAAAGTCTCCTATGCTTTCAATTCTTTCTTCCAAAGCAGAGACCAAGACGTTCGGACCTGCAATTTTGATGGCAAGGGAACGGTTACAAAAGACGATCCTTCTAAAGGAACTTGCGTATTCAATATACAGATTTACAGTGCTGGAGTGAGGCATCAAGTGACTTATGCATTGAAAAAGCTACTCAATAGAGGGCACCCAAATGTCTTAAGCGGATTGTGCAGGCGCAAGTACGAGTATGTCAAGTCATACGAAGTTGAAGATGAGATCTTCTTGCCTAATTTGATTGTTGTTCGAATTGATGGCTGTGATTTTCGAAGGTTTGCAGAAATGCATGAGTTTGAGAAACCAAATGATGAGGTAGCATTGAATTTGATGAATTCATGCGCAGTTTCTGTGTCGGAGGATTTTCCAGACATCGTTTTTTCATATGGATTTAGTGACGAGTACAGCTTTGTCTTCAAGAAGACGTCCAAGTTTCACCAGAGGCGTGCCAGCAAAGTAGAGTCTATTATAGTATCTTTCTTCAGCTCAGTATATTCTTATCTTTTGTGGCGACAAAATGATTGTCATACAAATAACCTGTGTAATACTTGCCTATGGGAGCTAATAATTAAAGGTGGAAAGACTGAAAGCGAAGCACTAGAGTTACTAGAGGGAACCAGTAAAGAAGAGAAAAACAACCTTCTTTTTGAAAAGTTTGAGATCAATTACCAAAAGTTAAATGAAATGTTTTGTCAAGGTTCCTGCATTTTGAAAACTGAGGTGATTGACGTAGTAAAGCACAATGAGAATGGCTCTCCTGTCCGGAGACTTAGGAAAAAGTTAAGAATAGTCCATTCAAAGAATATAGCGGGGATAAGCTTTTGGAACAAGCACAAATGTCTTTGGAATGAGATTTTCTCAAAAGTTGAACCAGATTTTCTCAAATCCTTCCAGTTTGAAAAAAGATTGATGCCATCGACTTGGATTGTCATTAGAATTGATGGTTGCCATTTCCACAGGTTTTCTGATGTTCATGAATTTGTGAAGCCAAATGATGAGCAGGCTCTCAAGCTTATGGATTCATGTGCAGTGGCTGTGGTAAAAGAGTTTCAAGACACAGTGTTTGCCTATGGCGTCAGTGATGAATACAGCTTTGTATTGAAGAAAGACTCTCAGTTCTATCAAAGGCGAGCAAGTAAAATAGTATCTGTCATGGTGTCACTCTTTACTTCCATGTACACAATGAAATGGAAAGATTTCTTCCCTGAGAGAGATTTGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000086 GO:0000166 GO:0000278 GO:0000287 GO:0000966 GO:0001882 GO:0001883 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007049 GO:0008033 GO:0008144 GO:0008150 GO:0008152 GO:0008192 GO:0008193 GO:0008283 GO:0009451 GO:0009987 GO:0010467 GO:0016043 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0017076 GO:0019001 GO:0022402 GO:0022607 GO:0030554 GO:0031974 GO:0031981 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032559 GO:0032561 GO:0033554 GO:0034470 GO:0034471 GO:0034641 GO:0034660 GO:0035639 GO:0036094 GO:0042802 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0044839 GO:0046483 GO:0046872 GO:0050896 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0051716 GO:0065003 GO:0070013 GO:0070568 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097367 GO:0099116 GO:1901265 GO:1901360 GO:1901363 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Thg1 Thg1C

Protein Analysis

829

Amino Acids

94.34

Weight (kDa)

8.46

Isoelectric Point (pI)

36.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_17	PF00332	26 - 344	1.2e-61	Glycosyl hydrolases family 17
X8	PF07983	366 - 435	2.1e-13	X8 domain
Thg1	PF04446	485 - 593	3.5e-39	tRNAHis guanylyltransferase
Thg1C	PF14413	586 - 669	8.9e-28	Thg1 C terminal domain
Thg1	PF04446	715 - 827	2.2e-45	tRNAHis guanylyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001736)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64790
fragaria_vesca	FvH4_1g22980 FvH4_1g22990
malus_domestica	MD01G1021100.v1.1 MD01G1021200.v1.1 MD15G1330800.v1.1 MD15G1330900.v1.1
prunus_persica	Prupe.6G168700_v2.0.a1 Prupe.6G168800_v2.0.a1
pyrus_communis	pycom01g05030 pycom15g29440
rosa_chinensis	RchiOBHm_Chr2g0117511 RchiOBHm_Chr2g0117521 RchiOBHm_Chr2g0117531
rosa_laevigata	RLG00000018351 RLG00000018352 RLG00000018353
rosa_multiflora	Rmu_co8432351.1_g000001 Rmu_sc0001617.1_g000014 Rmu_sc0003454.1_g000003 Rmu_sc0003454.1_g000004 Rmu_sc0003454.1_g000018 Rmu_sc0022810.1_g000001 Rmu_sc0039255.1_g000002
rosa_roxburghii	Rroxscaffold_2G00126440 Rroxscaffold_2G00126450 Rroxscaffold_2G00126480
rosa_rugosa	Rorug02G0212800 Rorug02G0212900 Rorug02G0213000
rosa_samantha	Rh2AG268100 Rh2AG268300 Rh2AG268400 Rh2BG280500 Rh2BG280700 Rh2BG280800 Rh2CG271500 Rh2CG271600 Rh2CG271700 Rh2DG275300 Rh2DG275500 Rh2DG275600 Rh2DG294800 Rh2DG295000 Rh2DG295100
rosa_wichuraiana	Rw2G021220 Rw2G021230 Rw2G021240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1694
Acc16I	TGCGCA	1 cut(s)	1609
Acc36I	ACCTGC	1 cut(s)	1289
AccB1I	GGYRCC	2 cut(s)	449, 1416
AccB7I	CCANNNNNTGG	1 cut(s)	778
AccIII	TCCGGA	1 cut(s)	2028
AciI	CCGC	4 cut(s)	800, 887, 1434, 2075
AclWI	GGATC	4 cut(s)	110, 123, 161, 1313
AcsI	RAATTY	7 cut(s)	234, 656, 837, 851, 1591, 1600, 2243
AcuI	CTGAAG	2 cut(s)	249, 1732
AcyI	GRCGYC	2 cut(s)	1691, 2340
AfaI	GTAC	8 cut(s)	676, 914, 926, 1071, 1085, 1454, 1670, 2444
AfiI	CCNNNNNNNGG	8 cut(s)	207, 288, 289, 524, 778, 815, 1044, 1328
AflII	CTTAAG	1 cut(s)	1429
AflIII	ACRYGT	1 cut(s)	26
AjnI	CCWGG	2 cut(s)	771, 967
AloI	GAACNNNNNNTCC	2 cut(s)	515, 547
Alw26I	GTCTC	7 cut(s)	16, 597, 632, 687, 1234, 1257, 2026
AlwI	GGATC	4 cut(s)	110, 123, 161, 1313
AlwNI	CAGNNNCTG	3 cut(s)	185, 1616, 2300
Aor13HI	TCCGGA	1 cut(s)	2028
AoxI	GGCC	3 cut(s)	198, 252, 806
ApoI	RAATTY	7 cut(s)	234, 656, 837, 851, 1591, 1600, 2243
ArsI	GACNNNNNNTTYG	4 cut(s)	1774, 1806, 2312, 2344
Asp700I	GAANNNNTTC	3 cut(s)	170, 851, 2462
AspLEI	GCGC	3 cut(s)	984, 1449, 1610
AspS9I	GGNCC	2 cut(s)	1030, 1278
AsuC2I	CCSGG	2 cut(s)	208, 1039
AsuHPI	GGTGA	6 cut(s)	298, 304, 830, 1027, 1694, 1999
AsuII	TTCGAA	4 cut(s)	904, 1123, 1516, 1537
AvaII	GGWCC	2 cut(s)	1030, 1278
BaeGI	GKGCMC	2 cut(s)	480, 1419
BamHI	GGATCC	1 cut(s)	115
BanI	GGYRCC	2 cut(s)	449, 1416
BanII	GRGCYC	1 cut(s)	939
BarI	GAAGNNNNNNTAC	2 cut(s)	752, 784
BbsI	GAAGAC	2 cut(s)	1672, 1694
BcgI	CGANNNNNNTGC	2 cut(s)	84, 118
BciT130I	CCWGG	2 cut(s)	773, 969
BclI	TGATCA	1 cut(s)	316
BcnI	CCSGG	2 cut(s)	208, 1039
BcoDI	GTCTC	7 cut(s)	16, 597, 632, 687, 1234, 1257, 2026
BfaI	CTAG	5 cut(s)	437, 569, 678, 1865, 1874
BfoI	RGCGCY	1 cut(s)	985
BfrI	CTTAAG	1 cut(s)	1429
BfuAI	ACCTGC	1 cut(s)	1289
BglI	GCCNNNNNGGC	1 cut(s)	990
BglII	AGATCT	1 cut(s)	1488
Bme1390I	CCNGG	4 cut(s)	208, 773, 969, 1039
Bme18I	GGWCC	2 cut(s)	1030, 1278
BmgT120I	GGNCC	2 cut(s)	1030, 1278
BmiI	GGNNCC	6 cut(s)	117, 451, 895, 1418, 1882, 1966
BmrFI	CCNGG	4 cut(s)	208, 773, 969, 1039
BmrI	ACTGGG	2 cut(s)	97, 997
BmsI	GCATC	7 cut(s)	110, 126, 546, 715, 1002, 1384, 2166
BmuI	ACTGGG	2 cut(s)	97, 997
BpiI	GAAGAC	2 cut(s)	1672, 1694
BplI	GAGNNNNNCTC	4 cut(s)	2003, 2035, 2108, 2140
BpmI	CTGGAG	1 cut(s)	1386
Bpu14I	TTCGAA	4 cut(s)	904, 1123, 1516, 1537
BpuEI	CTTGAG	8 cut(s)	54, 125, 545, 563, 740, 854, 1046, 2258
BpuMI	CCSGG	2 cut(s)	208, 1039
BsaBI	GATNNNNATC	1 cut(s)	729
BsaHI	GRCGYC	2 cut(s)	1691, 2340
BsaI	GGTCTC	1 cut(s)	1257
BsaJI	CCNNGG	2 cut(s)	967, 968
BsaWI	WCCGGW	1 cut(s)	2028
Bsc4I	CCNNNNNNNGG	8 cut(s)	207, 288, 289, 524, 778, 815, 1044, 1328
Bse1I	ACTGG	4 cut(s)	92, 992, 1884, 2158
Bse3DI	GCAATG	4 cut(s)	102, 130, 413, 1103
Bse8I	GATNNNNATC	1 cut(s)	729
BseAI	TCCGGA	1 cut(s)	2028
BseBI	CCWGG	2 cut(s)	773, 969
BseDI	CCNNGG	2 cut(s)	967, 968
BseGI	GGATG	1 cut(s)	809
BseJI	GATNNNNATC	1 cut(s)	729
BseLI	CCNNNNNNNGG	8 cut(s)	207, 288, 289, 524, 778, 815, 1044, 1328
BseMI	GCAATG	4 cut(s)	102, 130, 413, 1103
BseMII	CTCAG	5 cut(s)	1181, 1767, 1974, 2393, 2463
BseNI	ACTGG	4 cut(s)	92, 992, 1884, 2158
BseSI	GKGCMC	2 cut(s)	480, 1419
BseYI	CCCAGC	1 cut(s)	119
BsgI	GTGCAG	2 cut(s)	1462, 2313
BshFI	GGCC	3 cut(s)	200, 254, 808
BshNI	GGYRCC	2 cut(s)	449, 1416
BsiSI	CCGG	3 cut(s)	207, 1038, 2029
BslFI	GGGAC	1 cut(s)	986
BslI	CCNNNNNNNGG	8 cut(s)	207, 288, 289, 524, 778, 815, 1044, 1328
BsmAI	GTCTC	7 cut(s)	16, 597, 632, 687, 1234, 1257, 2026
BsmFI	GGGAC	1 cut(s)	986
BsmI	GAATGC	2 cut(s)	662, 832
BsnI	GGCC	3 cut(s)	200, 254, 808
Bso31I	GGTCTC	1 cut(s)	1257
Bsp119I	TTCGAA	4 cut(s)	904, 1123, 1516, 1537
Bsp1286I	GDGCHC	3 cut(s)	480, 939, 1419
Bsp13I	TCCGGA	1 cut(s)	2028
Bsp1407I	TGTACA	2 cut(s)	1069, 2442
Bsp143I	GATC	6 cut(s)	115, 153, 316, 1318, 1488, 1926
BspACI	CCGC	4 cut(s)	800, 887, 1434, 2075
BspANI	GGCC	3 cut(s)	200, 254, 808
BspCNI	CTCAG	5 cut(s)	1180, 1766, 1975, 2392, 2464
BspEI	TCCGGA	1 cut(s)	2028
BspHI	TCATGA	2 cut(s)	733, 2239
BspLI	GGNNCC	6 cut(s)	117, 451, 895, 1418, 1882, 1966
BspMI	ACCTGC	1 cut(s)	1289
BspPI	GGATC	4 cut(s)	110, 123, 161, 1313
BspT104I	TTCGAA	4 cut(s)	904, 1123, 1516, 1537
BspT107I	GGYRCC	2 cut(s)	449, 1416
BspTI	CTTAAG	1 cut(s)	1429
BspTNI	GGTCTC	1 cut(s)	1257
BsrDI	GCAATG	4 cut(s)	102, 130, 413, 1103
BsrGI	TGTACA	2 cut(s)	1069, 2442
BsrI	ACTGG	4 cut(s)	92, 992, 1884, 2158
BssECI	CCNNGG	2 cut(s)	967, 968
BssMI	GATC	6 cut(s)	115, 153, 316, 1318, 1488, 1926
BssNI	GRCGYC	2 cut(s)	1691, 2340
Bst2UI	CCWGG	2 cut(s)	773, 969
Bst4CI	ACNGT	3 cut(s)	1306, 1361, 2326
Bst6I	CTCTTC	2 cut(s)	906, 1887
BstACI	GRCGYC	2 cut(s)	1691, 2340
BstAFI	CTTAAG	1 cut(s)	1429
BstAPI	GCANNNNNTGC	1 cut(s)	185
BstAUI	TGTACA	2 cut(s)	1069, 2442
BstBI	TTCGAA	4 cut(s)	904, 1123, 1516, 1537
BstC8I	GCNNGC	7 cut(s)	186, 448, 1445, 1713, 1717, 2268, 2397
BstDEI	CTNAG	8 cut(s)	623, 997, 1167, 1753, 1983, 2036, 2379, 2472
BstEII	GGTNACC	1 cut(s)	767
BstENI	CCTNNNNNAGG	1 cut(s)	1326
BstF5I	GGATG	1 cut(s)	809
BstH2I	RGCGCY	1 cut(s)	985
BstHHI	GCGC	3 cut(s)	984, 1449, 1610
BstKTI	GATC	6 cut(s)	118, 156, 319, 1321, 1491, 1929
BstMAI	GTCTC	7 cut(s)	16, 597, 632, 687, 1234, 1257, 2026
BstMBI	GATC	6 cut(s)	115, 153, 316, 1318, 1488, 1926
BstMWI	GCNNNNNNNGC	4 cut(s)	185, 990, 999, 1440
BstNI	CCWGG	2 cut(s)	773, 969
BstNSI	RCATGY	2 cut(s)	30, 281
BstPI	GGTNACC	1 cut(s)	767
BstSCI	CCNGG	4 cut(s)	206, 771, 967, 1037
BstSLI	GKGCMC	2 cut(s)	480, 1419
BstV2I	GAAGAC	2 cut(s)	1672, 1694
BstX2I	RGATCY	2 cut(s)	115, 1488
BstXI	CCANNNNNNTGG	1 cut(s)	2187
BstYI	RGATCY	2 cut(s)	115, 1488
BsuRI	GGCC	3 cut(s)	200, 254, 808
BtgZI	GCGATG	2 cut(s)	87, 1131
BtsCI	GGATG	1 cut(s)	809
BtsI	GCAGTG	1 cut(s)	2301
BtsIMutI	CAGTG	6 cut(s)	939, 985, 1366, 2301, 2331, 2350
BveI	ACCTGC	1 cut(s)	1289
Cac8I	GCNNGC	7 cut(s)	186, 448, 1445, 1713, 1717, 2268, 2397
CaiI	CAGNNNCTG	3 cut(s)	185, 1616, 2300
CciI	TCATGA	2 cut(s)	733, 2239
CfoI	GCGC	3 cut(s)	984, 1449, 1610
Cfr13I	GGNCC	2 cut(s)	1030, 1278
CseI	GACGC	2 cut(s)	185, 2329
Csp6I	GTAC	8 cut(s)	675, 913, 925, 1070, 1084, 1453, 1669, 2443
CviQI	GTAC	8 cut(s)	675, 913, 925, 1070, 1084, 1453, 1669, 2443
DdeI	CTNAG	8 cut(s)	623, 997, 1167, 1753, 1983, 2036, 2379, 2472
DpnI	GATC	6 cut(s)	117, 155, 318, 1320, 1490, 1928
DpnII	GATC	6 cut(s)	115, 153, 316, 1318, 1488, 1926
Eam1104I	CTCTTC	2 cut(s)	906, 1887
EarI	CTCTTC	2 cut(s)	906, 1887
EciI	GGCGGA	1 cut(s)	902
Eco147I	AGGCCT	1 cut(s)	200
Eco24I	GRGCYC	1 cut(s)	939
Eco31I	GGTCTC	1 cut(s)	1257
Eco32I	GATATC	1 cut(s)	337
Eco47I	GGWCC	2 cut(s)	1030, 1278
Eco57I	CTGAAG	2 cut(s)	249, 1732
Eco91I	GGTNACC	1 cut(s)	767
EcoNI	CCTNNNNNAGG	1 cut(s)	1326
EcoO65I	GGTNACC	1 cut(s)	767
EcoRI	GAATTC	2 cut(s)	234, 1600
EcoRII	CCWGG	2 cut(s)	771, 967
EcoRV	GATATC	1 cut(s)	337
EcoT22I	ATGCAT	2 cut(s)	1393, 1557
EcoT38I	GRGCYC	1 cut(s)	939
FalI	AAGNNNNNCTT	4 cut(s)	153, 185, 912, 944
FaqI	GGGAC	1 cut(s)	986
FauI	CCCGC	2 cut(s)	793, 2068
FauNDI	CATATG	1 cut(s)	1651
FbaI	TGATCA	1 cut(s)	316
FokI	GGATG	1 cut(s)	816
FriOI	GRGCYC	1 cut(s)	939
FspBI	CTAG	5 cut(s)	437, 569, 678, 1865, 1874
FspI	TGCGCA	1 cut(s)	1609
GlaI	GCGC	3 cut(s)	983, 1448, 1609
GsaI	CCCAGC	1 cut(s)	123
GsuI	CTGGAG	1 cut(s)	1386
HaeII	RGCGCY	1 cut(s)	985
HaeIII	GGCC	3 cut(s)	200, 254, 808
HapII	CCGG	3 cut(s)	207, 1038, 2029
HgaI	GACGC	2 cut(s)	185, 2329
HhaI	GCGC	3 cut(s)	984, 1449, 1610
Hin1I	GRCGYC	2 cut(s)	1691, 2340
Hin6I	GCGC	3 cut(s)	982, 1447, 1608
HinP1I	GCGC	3 cut(s)	982, 1447, 1608
HincII	GTYRAC	2 cut(s)	85, 607
HindII	GTYRAC	2 cut(s)	85, 607
HindIII	AAGCTT	3 cut(s)	167, 2083, 2276
HinfI	GANTC	5 cut(s)	9, 500, 1727, 2285, 2375
HpaI	GTTAAC	1 cut(s)	607
HpaII	CCGG	3 cut(s)	207, 1038, 2029
HphI	GGTGA	6 cut(s)	298, 304, 830, 1027, 1694, 1999
Hpy166II	GTNNAC	5 cut(s)	85, 496, 607, 925, 2445
Hpy188I	TCNGA	6 cut(s)	523, 536, 1170, 1278, 1624, 2233
Hpy8I	GTNNAC	5 cut(s)	85, 496, 607, 925, 2445
Hpy99I	CGWCG	1 cut(s)	179
HpyCH4III	ACNGT	3 cut(s)	1306, 1361, 2326
HpyCH4IV	ACGT	3 cut(s)	1272, 1691, 1995
HpyF10VI	GCNNNNNNNGC	4 cut(s)	185, 990, 999, 1440
HpyF3I	CTNAG	8 cut(s)	623, 997, 1167, 1753, 1983, 2036, 2379, 2472
HpySE526I	ACGT	3 cut(s)	1272, 1691, 1995
Hsp92I	GRCGYC	2 cut(s)	1691, 2340
HspAI	GCGC	3 cut(s)	982, 1447, 1608
Kpn2I	TCCGGA	1 cut(s)	2028
Ksp22I	TGATCA	1 cut(s)	316
KspAI	GTTAAC	1 cut(s)	607
Kzo9I	GATC	6 cut(s)	115, 153, 316, 1318, 1488, 1926
LmnI	GCTCC	2 cut(s)	92, 1825
LweI	GCATC	7 cut(s)	110, 126, 546, 715, 1002, 1384, 2166
MaeI	CTAG	5 cut(s)	437, 569, 678, 1865, 1874
MaeII	ACGT	3 cut(s)	1272, 1691, 1995
MalI	GATC	6 cut(s)	117, 155, 318, 1320, 1490, 1928
MboI	GATC	6 cut(s)	115, 153, 316, 1318, 1488, 1926
MflI	RGATCY	2 cut(s)	115, 1488
MhlI	GDGCHC	3 cut(s)	480, 939, 1419
MlyI	GAGTC	3 cut(s)	18, 1736, 2369
MmeI	TCCRAC	4 cut(s)	211, 334, 655, 1602
Mph1103I	ATGCAT	2 cut(s)	1393, 1557
MroI	TCCGGA	1 cut(s)	2028
MroXI	GAANNNNTTC	3 cut(s)	170, 851, 2462
MseI	TTAA	6 cut(s)	371, 606, 1430, 1836, 1943, 2048
MslI	CAYNNNNRTG	1 cut(s)	1422
MspCI	CTTAAG	1 cut(s)	1429
MspI	CCGG	3 cut(s)	207, 1038, 2029
MspR9I	CCNGG	4 cut(s)	208, 773, 969, 1039
Mva1269I	GAATGC	2 cut(s)	662, 832
MvaI	CCWGG	2 cut(s)	773, 969
MwoI	GCNNNNNNNGC	4 cut(s)	185, 990, 999, 1440
NciI	CCSGG	2 cut(s)	208, 1039
NdeI	CATATG	1 cut(s)	1651
NdeII	GATC	6 cut(s)	115, 153, 316, 1318, 1488, 1926
NlaIV	GGNNCC	6 cut(s)	117, 451, 895, 1418, 1882, 1966
NmuCI	GTSAC	5 cut(s)	750, 818, 1381, 1661, 2424
NsbI	TGCGCA	1 cut(s)	1609
NsiI	ATGCAT	2 cut(s)	1393, 1557
NspI	RCATGY	2 cut(s)	30, 281
NspV	TTCGAA	4 cut(s)	904, 1123, 1516, 1537
PagI	TCATGA	2 cut(s)	733, 2239
PasI	CCCWGGG	1 cut(s)	968
PceI	AGGCCT	1 cut(s)	200
PciI	ACATGT	1 cut(s)	26
PctI	GAATGC	2 cut(s)	662, 832
PdmI	GAANNNNTTC	3 cut(s)	170, 851, 2462
PfeI	GAWTC	2 cut(s)	500, 2285
PflMI	CCANNNNNTGG	1 cut(s)	778
PleI	GAGTC	3 cut(s)	17, 1735, 2369
PpsI	GAGTC	3 cut(s)	17, 1735, 2369
PscI	ACATGT	1 cut(s)	26
Psp6I	CCWGG	2 cut(s)	771, 967
PspEI	GGTNACC	1 cut(s)	767
PspFI	CCCAGC	1 cut(s)	119
PspGI	CCWGG	2 cut(s)	771, 967
PspN4I	GGNNCC	6 cut(s)	117, 451, 895, 1418, 1882, 1966
PspPI	GGNCC	2 cut(s)	1030, 1278
PstNI	CAGNNNCTG	3 cut(s)	185, 1616, 2300
PsuI	RGATCY	2 cut(s)	115, 1488
RsaI	GTAC	8 cut(s)	676, 914, 926, 1071, 1085, 1454, 1670, 2444
RsaNI	GTAC	8 cut(s)	675, 913, 925, 1070, 1084, 1453, 1669, 2443
RseI	CAYNNNNRTG	1 cut(s)	1422
SaqAI	TTAA	6 cut(s)	371, 606, 1430, 1836, 1943, 2048
Sau3AI	GATC	6 cut(s)	115, 153, 316, 1318, 1488, 1926
Sau96I	GGNCC	2 cut(s)	1030, 1278
SchI	GAGTC	3 cut(s)	18, 1736, 2369
ScrFI	CCNGG	4 cut(s)	208, 773, 969, 1039
SduI	GDGCHC	3 cut(s)	480, 939, 1419
SfaNI	GCATC	7 cut(s)	110, 126, 546, 715, 1002, 1384, 2166
SfuI	TTCGAA	4 cut(s)	904, 1123, 1516, 1537
SinI	GGWCC	2 cut(s)	1030, 1278
SmiMI	CAYNNNNRTG	1 cut(s)	1422
SmlI	CTYRAG	9 cut(s)	69, 140, 560, 578, 755, 869, 1061, 1429, 2273
SmoI	CTYRAG	9 cut(s)	69, 140, 560, 578, 755, 869, 1061, 1429, 2273
SseBI	AGGCCT	1 cut(s)	200
SsiI	CCGC	4 cut(s)	800, 887, 1434, 2075
SspMI	CTAG	5 cut(s)	437, 569, 678, 1865, 1874
StuI	AGGCCT	1 cut(s)	200
StyD4I	CCNGG	4 cut(s)	206, 771, 967, 1037
TaaI	ACNGT	3 cut(s)	1306, 1361, 2326
TaiI	ACGT	3 cut(s)	1275, 1694, 1998
TaqI	TCGA	8 cut(s)	7, 174, 691, 904, 1123, 1516, 1537, 2183
TaqII	GACCGA	1 cut(s)	211
TatI	WGTACW	4 cut(s)	1069, 1083, 1668, 2442
TfiI	GAWTC	2 cut(s)	500, 2285
Tru1I	TTAA	6 cut(s)	371, 606, 1430, 1836, 1943, 2048
Tru9I	TTAA	6 cut(s)	371, 606, 1430, 1836, 1943, 2048
TscAI	CASTG	6 cut(s)	946, 992, 1366, 2301, 2331, 2350
TseFI	GTSAC	5 cut(s)	750, 818, 1381, 1661, 2424
Tsp45I	GTSAC	5 cut(s)	750, 818, 1381, 1661, 2424
TspGWI	ACGGA	1 cut(s)	906
TspRI	CASTG	6 cut(s)	946, 992, 1366, 2301, 2331, 2350
Van91I	CCANNNNNTGG	1 cut(s)	778
Vha464I	CTTAAG	1 cut(s)	1429
VpaK11BI	GGWCC	2 cut(s)	1030, 1278
XagI	CCTNNNNNAGG	1 cut(s)	1326
XapI	RAATTY	7 cut(s)	234, 656, 837, 851, 1591, 1600, 2243
XbaI	TCTAGA	1 cut(s)	436
XceI	RCATGY	2 cut(s)	30, 281
XcmI	CCANNNNNNNNNTGG	2 cut(s)	247, 1024
XmnI	GAANNNNTTC	3 cut(s)	170, 851, 2462
XspI	CTAG	5 cut(s)	437, 569, 678, 1865, 1874
ZraI	GACGTC	1 cut(s)	1692
Zsp2I	ATGCAT	2 cut(s)	1393, 1557

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.