Rh2DG234500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0009739
Physical Location & Seq
Reverse (-)
23322158 .. 23349062
26905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG234500.1

Sequence Viewer

Length: 522 bp
ATGAGTTCGGCGGTGCTGAAGGAGAGAAGAAGGCCAAATGCTCTGACTGTGTCCCTTAATTCGAAGATTTACAGAGTCAGCGATAGGCTCTTCCTCTGCCTCTCCGGCCTCACCACTGATGCTCAGACTCTGTCTGAGTGGTGGTGCTGGTGGAATATGATTTATCTCATTTTGAAATTAAATGTCAACCTATTGTGGACTTGTTTAACTTTAAGAGGTCATCTCCGGAGTCGCAGGGATGTTGAAGGCTCTCTCTTTGTCATTAAGAGATTCGCATGCATCAGCCTCTTTCCGAGTCGCCGGAGACGCCGTATGCCCGTGTGCTCCCAAGGTATTTCAACTCATAATGGTCAGTGGCCAAATTTCACTTGCAGGAAGGTCTGCAGCATATTGTTGCCTTTGGCAAGCGGAAGAATACCATCATTATCATTGGCATGGATGGAAGAAGAAAATGATGCAAAAAATGAAGAGGCTGATATGGAGGAGCTCATCAAAGCAGTTGCAGATCAGGTGGCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

19.96

Weight (kDa)

9.53

Isoelectric Point (pI)

66.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 225
AciI CCGC 2 cut(s) 11, 408
AcoI YGGCCR 1 cut(s) 356
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 307
AgsI TTSAA 3 cut(s) 175, 245, 339
AluBI AGCT 1 cut(s) 487
AluI AGCT 1 cut(s) 487
Alw21I GWGCWC 2 cut(s) 326, 489
Alw26I GTCTC 1 cut(s) 298
AlwNI CAGNNNCTG 1 cut(s) 130
Aor13HI TCCGGA 1 cut(s) 225
AoxI GGCC 3 cut(s) 32, 106, 356
ApeKI GCWGC 1 cut(s) 384
ApoI RAATTY 1 cut(s) 361
AsuHPI GGTGA 1 cut(s) 103
AsuII TTCGAA 1 cut(s) 62
BalI TGGCCA 1 cut(s) 358
BanII GRGCYC 1 cut(s) 489
Bbv12I GWGCWC 2 cut(s) 326, 489
BbvI GCAGC 1 cut(s) 396
BccI CCATC 2 cut(s) 427, 433
BceAI ACGGC 1 cut(s) 294
BcoDI GTCTC 1 cut(s) 298
BfmI CTRYAG 1 cut(s) 382
BglI GCCNNNNNGGC 1 cut(s) 105
BisI GCNGC 1 cut(s) 385
BlsI GCNGC 1 cut(s) 386
BmsI GCATC 3 cut(s) 109, 288, 445
BplI GAGNNNNNCTC 2 cut(s) 207, 239
Bpu14I TTCGAA 1 cut(s) 62
BsaHI GRCGYC 1 cut(s) 307
BsaJI CCNNGG 1 cut(s) 328
BsaWI WCCGGW 1 cut(s) 225
BsaXI ACNNNNNCTCC 2 cut(s) 295, 325
BseAI TCCGGA 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 328
BseGI GGATG 2 cut(s) 244, 444
BseMII CTCAG 2 cut(s) 126, 137
BseRI GAGGAG 1 cut(s) 497
BseXI GCAGC 1 cut(s) 396
BshFI GGCC 3 cut(s) 34, 108, 358
BsiHKAI GWGCWC 2 cut(s) 326, 489
BsiSI CCGG 3 cut(s) 105, 226, 301
BslFI GGGAC 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 298
BsmBI CGTCTC 1 cut(s) 298
BsmFI GGGAC 1 cut(s) 37
BsnI GGCC 3 cut(s) 34, 108, 358
Bsp119I TTCGAA 1 cut(s) 62
Bsp1286I GDGCHC 2 cut(s) 326, 489
Bsp13I TCCGGA 1 cut(s) 225
Bsp143I GATC 1 cut(s) 505
BspACI CCGC 2 cut(s) 11, 408
BspANI GGCC 3 cut(s) 34, 108, 358
BspCNI CTCAG 2 cut(s) 127, 136
BspEI TCCGGA 1 cut(s) 225
BspMAI CTGCAG 1 cut(s) 386
BspQI GCTCTTC 1 cut(s) 95
BspT104I TTCGAA 1 cut(s) 62
BssECI CCNNGG 1 cut(s) 328
BssMI GATC 1 cut(s) 505
BssNI GRCGYC 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 328
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 2 cut(s) 95, 462
BstACI GRCGYC 1 cut(s) 307
BstBI TTCGAA 1 cut(s) 62
BstC8I GCNNGC 2 cut(s) 277, 406
BstDEI CTNAG 2 cut(s) 123, 135
BstF5I GGATG 2 cut(s) 244, 444
BstKTI GATC 1 cut(s) 508
BstMAI GTCTC 1 cut(s) 298
BstMBI GATC 1 cut(s) 505
BstMWI GCNNNNNNNGC 2 cut(s) 105, 306
BstNSI RCATGY 1 cut(s) 279
BstSFI CTRYAG 1 cut(s) 382
BstV1I GCAGC 1 cut(s) 396
BsuRI GGCC 3 cut(s) 34, 108, 358
BtsCI GGATG 2 cut(s) 244, 444
BtsIMutI CAGTG 2 cut(s) 114, 359
Cac8I GCNNGC 2 cut(s) 277, 406
CaiI CAGNNNCTG 1 cut(s) 130
CseI GACGC 1 cut(s) 315
CviAII CATG 2 cut(s) 276, 435
CviJI RGCY 8 cut(s) 34, 88, 108, 249, 285, 358, 473, 487
CviKI_1 RGCY 8 cut(s) 34, 88, 108, 249, 285, 358, 473, 487
DdeI CTNAG 2 cut(s) 123, 135
DpnI GATC 1 cut(s) 507
DpnII GATC 1 cut(s) 505
EaeI YGGCCR 1 cut(s) 356
Eam1104I CTCTTC 2 cut(s) 95, 462
EarI CTCTTC 2 cut(s) 95, 462
Ecl136II GAGCTC 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 328
Eco24I GRGCYC 1 cut(s) 489
Eco53kI GAGCTC 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 38
EcoICRI GAGCTC 1 cut(s) 487
EcoT14I CCWWGG 1 cut(s) 328
EcoT22I ATGCAT 1 cut(s) 281
EcoT38I GRGCYC 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 328
Esp3I CGTCTC 1 cut(s) 298
FaeI CATG 2 cut(s) 279, 438
FaiI YATR 7 cut(s) 158, 277, 314, 345, 389, 436, 479
FaqI GGGAC 1 cut(s) 37
FatI CATG 2 cut(s) 275, 434
Fnu4HI GCNGC 1 cut(s) 385
FokI GGATG 2 cut(s) 251, 451
FriOI GRGCYC 1 cut(s) 489
Fsp4HI GCNGC 1 cut(s) 385
GluI GCNGC 1 cut(s) 385
HaeIII GGCC 3 cut(s) 34, 108, 358
HapII CCGG 3 cut(s) 105, 226, 301
HgaI GACGC 1 cut(s) 315
Hin1I GRCGYC 1 cut(s) 307
Hin1II CATG 2 cut(s) 279, 438
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 5 cut(s) 75, 127, 229, 270, 295
HpaII CCGG 3 cut(s) 105, 226, 301
HphI GGTGA 1 cut(s) 103
Hpy166II GTNNAC 2 cut(s) 187, 198
Hpy188I TCNGA 4 cut(s) 45, 126, 136, 294
Hpy188III TCNNGA 1 cut(s) 226
Hpy8I GTNNAC 2 cut(s) 187, 198
HpyAV CCTTC 4 cut(s) 13, 24, 239, 370
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4V TGCA 5 cut(s) 279, 372, 384, 458, 503
HpyF10VI GCNNNNNNNGC 2 cut(s) 105, 306
HpyF3I CTNAG 2 cut(s) 123, 135
Hsp92I GRCGYC 1 cut(s) 307
Hsp92II CATG 2 cut(s) 279, 438
Kpn2I TCCGGA 1 cut(s) 225
Kzo9I GATC 1 cut(s) 505
LguI GCTCTTC 1 cut(s) 95
LmnI GCTCC 2 cut(s) 329, 484
LpnPI CCDG 7 cut(s) 118, 133, 220, 239, 314, 358, 494
Lsp1109I GCAGC 1 cut(s) 396
LweI GCATC 3 cut(s) 109, 288, 445
MalI GATC 1 cut(s) 507
MboI GATC 1 cut(s) 505
MboII GAAGA 7 cut(s) 39, 76, 82, 423, 455, 458, 479
MhlI GDGCHC 2 cut(s) 326, 489
MlsI TGGCCA 1 cut(s) 358
MluCI AATT 3 cut(s) 58, 176, 361
MluNI TGGCCA 1 cut(s) 358
MlyI GAGTC 4 cut(s) 84, 121, 238, 304
MnlI CCTC 7 cut(s) 104, 110, 119, 209, 296, 463, 475
Mox20I TGGCCA 1 cut(s) 358
Mph1103I ATGCAT 1 cut(s) 281
MroI TCCGGA 1 cut(s) 225
MscI TGGCCA 1 cut(s) 358
MseI TTAA 5 cut(s) 57, 179, 206, 212, 264
MslI CAYNNNNRTG 1 cut(s) 433
Msp20I TGGCCA 1 cut(s) 358
MspI CCGG 3 cut(s) 105, 226, 301
MwoI GCNNNNNNNGC 2 cut(s) 105, 306
NdeII GATC 1 cut(s) 505
NlaIII CATG 2 cut(s) 279, 438
NsiI ATGCAT 1 cut(s) 281
NspI RCATGY 1 cut(s) 279
NspV TTCGAA 1 cut(s) 62
PaeI GCATGC 1 cut(s) 279
PciSI GCTCTTC 1 cut(s) 95
PfeI GAWTC 1 cut(s) 270
PflFI GACNNNGTC 2 cut(s) 49, 130
PkrI GCNGC 1 cut(s) 386
PleI GAGTC 4 cut(s) 83, 121, 237, 303
PpsI GAGTC 4 cut(s) 83, 121, 237, 303
Psp124BI GAGCTC 1 cut(s) 489
PstI CTGCAG 1 cut(s) 386
PstNI CAGNNNCTG 1 cut(s) 130
PsyI GACNNNGTC 2 cut(s) 49, 130
RseI CAYNNNNRTG 1 cut(s) 433
SacI GAGCTC 1 cut(s) 489
SapI GCTCTTC 1 cut(s) 95
SaqAI TTAA 5 cut(s) 57, 179, 206, 212, 264
SatI GCNGC 1 cut(s) 385
Sau3AI GATC 1 cut(s) 505
SchI GAGTC 4 cut(s) 84, 121, 238, 304
SduI GDGCHC 2 cut(s) 326, 489
SetI ASST 6 cut(s) 192, 220, 334, 381, 489, 513
SfaNI GCATC 3 cut(s) 109, 288, 445
SfcI CTRYAG 1 cut(s) 382
SfuI TTCGAA 1 cut(s) 62
SmiMI CAYNNNNRTG 1 cut(s) 433
SphI GCATGC 1 cut(s) 279
Sse9I AATT 3 cut(s) 58, 176, 361
SsiI CCGC 2 cut(s) 11, 408
SstI GAGCTC 1 cut(s) 489
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 1 cut(s) 49
TaqI TCGA 1 cut(s) 62
TasI AATT 3 cut(s) 58, 176, 361
TfiI GAWTC 1 cut(s) 270
Tru1I TTAA 5 cut(s) 57, 179, 206, 212, 264
Tru9I TTAA 5 cut(s) 57, 179, 206, 212, 264
TscAI CASTG 2 cut(s) 121, 359
TseI GCWGC 1 cut(s) 384
TspDTI ATGAA 1 cut(s) 480
TspRI CASTG 2 cut(s) 121, 359
Tth111I GACNNNGTC 2 cut(s) 49, 130
XapI RAATTY 1 cut(s) 361
XceI RCATGY 1 cut(s) 279
Zsp2I ATGCAT 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.