Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0002843

Physical Location & Seq

Genomic Coordinates Reverse (-)

59219637 .. 59236554

Gene Structure 16918 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG408700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3534 bp

ATGAATATGGAACAAGATTCTGAATTTAAGTGGGGTGAAAGACTTGGAGTTGGTAGGACAAACAAGGATGTCCGGTTCTACCAGTCTTTCACATTTCGGGGCGTGGAGTACTCCCTGTATGATTCCGTACACCTCTACAAGACTGGCGCTTTGGAAACAGATATTGGTAAGATTATCAAAATTTATTCCACAGCAACCGAAAAGAAGGTTAGGATTGTGTGGTTTCTTCGGCCAGCTGAAATATGCAACTTTTTGGGTGATTATGAACCACACTGGAAAGAGATTTTTTTAGCTTCTGGCGAGGGTACAGGCCTCTCCAATCTGAATCCTCTGGAAGCAATTGTTGGAAAGTGTAATGTTGTCTGCGTATCAAAGGATACAAGGAATCCTCGTCACTCTAAAGAAGAGTTGAGAACGGCCAACTACTTTTTCCGTCGTACGTTTGACGTTGACAGGCGCAAAATATTGCAGGAGTTTCCGGATCAAATATATGGAATTAAAGTAGAGAAATATTTTAACAGGCGAAAACAGCAAAGCCTCACCAGGATGGACCAAGCTGGACCATCAAATGTGCCTTCCAATTCACAATTGGACAAAGTGGTGGCATATCCAGTTAAAAATGGCAAGCCTAGTAGTAAATCTATCCGTATAGTCAAAGAATCGGAGAGTCGGCGTCTAGAAGCTGGACCATCAAGTGTGCCTTCCAATTCACAATTAAGCAAAGTGGTGTTGTGTCCACTCCTGAATGTTAAAAGAAATACAGTGGACCCTTTACTACCACCTGTGAGCACACCAGATACCCGTCCTTATAAGAAGATGAAAACTGCAGATAGTAGACCGCCAGATATTGATAAGAGTGGTTGGTTCAAGCCAGCATTTTGGGAGGAAAGACTGCGGACAGCTGAAGGTAATGGCACCTTGGTCCTGCTAGACAATCTAGATCCATCCTTTACATCCTCCGAAGTACAGGATCTAGTTTGGGCTGCATTCAAGCAGATGGTTGAGGCAAAAATGATACCATGCACTCCTTTTTCTAATCCACGCTATGGAAAGGCTTTTGTTATATTTAAGTCGTCAGATGATGCTGAATCTGCTATAGTTGAGTTAAATAGGAGATGCCTGACTCTGGCAGATGGGAGACCTGTCATCGGTACCATCTGCAGAGGGACTCTAAAAGTGCCTGACAAAATATCCAGATTTTATGGACATCTGACGATTGACAAACATCGGCTTAAAAGCCAACGTGAAGAGATGAGAAAAGCAGTATCAACGTCGCACTTTGCTCAGCCCAATACAGTTGAGTATGATATGGCAATGGACTGGTGTTTACTGCAGGAAAGGTCAGATTTGTGGTGGAAGGCTTTATATCAGGACCATGAGGAAGAAGAGAAGGAGATAAAGAAGTTGGGAGAGCAAAAGGAGAAATTTTTTGTTCCCTGTGGATACAAAATTACAGTACAGTCAGATAGGGCACCATTTTCCGGCCAAAACTCAGGCCGGTCACTTCTTCGGCCTACCATTTCTTCCGAAATGGAGAGCTTCAAAGCCCCATTCAGGGGTATTGCAAGCGATATTCGAGGAAGAGCAGCATGCTACAAGAAAGATTGGATTGGAGGCATTCGTTCAGGAATCGGGATATTGGCCCCAACTACATACATCTTCTTTGCTTCAGCTCTTCCTGTTATAGCATTTGGGGAGCAACTGAGTAGAGATACAGATGGAAGCTTGAGCACAGTTGAAACATTGGCTTCTACAGCTATCTGTGGCATAATACACTCCATTCTGGGTGGGCAACCTCTATTGATTTTGGGAGTTGCAGAACCGACTATTATAATGTACACGTACTTGTACGACTTTGCAAAAGGAAAGGAAGGTCTGGGACGCGAACTGTTTCTAGCTTGGGTTGGATGGATTTGCGTCTGGACATCCCTTTTGCTCATTCTTCTGGCAATATTCAATGTTTGTGACATCATCAATAAGTTTACGAGGATTGCAGGGGAACTTTTTGGCATGTTGATAAATGTTCTGTTCATTCAGCAGGCTATCAAGGGAATAGTGAGTGAGTTCAAGCTACAGAAAGGTGAAGATCCAAATGGGGGGAGTTATCAACGTGAGTCAATGTACACAAATGGACTTCTGGGGGTCATATTTACTGTTGGTGTCCTCTATACTTCATTGAAGACCAGAAAAGCAAGATCATGGTGGTATGCAACAGGGTGGTTCAGAAGTTTCATTGCAGATTACGGGGTTCCTCTAATGGTAGTGTTGTGGACTGTTATATCATTCAGTGTACCACACAATATTGTTCCCTCACAAGTTCCTAGAAGGCTATATAGCCCTCTAGCATGGGATTCTGAATCTTTACACCATTGGTCTGTAATCAAGGATATGGGGAAGGTTTCTCCTGGATACATCTTTGCCGCTTTCATTCCTGCTCTGATGGTAGCGGGTCTCTACTTTTTTGACCACAGTGTTGCTTCACAAATGGCACAACAAAAGGAGTTCAATCTAAAGAAACCTTCTGCATACCATTATGATATTTTGCTGCTGGGATTTTTGACACTGCTTTGTGGATTGATTGGGCTTCCTCCTTGTAATGGTGTCCTGCCACAGGCACCCATGCACACTAAGAGCCTTGCAGTTCTTAAGAGGCAGTTGATTCGAAAGGAAATGGTAAAGAGTGCTAAAGAAAGCATAAAACAGAAAGCTAGCAATTCTGAAATCTATGGAAATATGCAAGCTATATTCATAGAGATGGACAACTCGACTGCAGATACTTCAGTAGTTACAGAGCTTGAAGACTTGAAGGAGGCAGTTATGAAAAGTGAACATGAAGGGGAGAATGCAAAAGATGCATTTGATCCTGAGAAGCACATTGATGCTTTCCTTCCTGTCCGGGTTAACGAGCAAAGAGTAAGCAATCTGTTGCAGTCACTTCTTGTAGCAGCATCAGTTTTTGCTATGCCAGCCATCAAGAAGATACCAACATCAGTTTTGTGGGGATACTTTGCTTACATGGCCATTGACAGTCTTCCGGGGAATCAATTCTGGGAAAGGTTGCTATTTCTCTTCATTGCGCCTAGCCGACGTTACAAGGTCTTGGAAGGGGTTCATGCTTCCTTTATGGAGTCAGTGCCATTCAAATACATTGCCATATTTACACTCTTTCAGCTTGTATACTTCCTGTTCTGCTTTGGTGTGACATGGATCCCCATAGCCGGCATTTTATTCCCCTTGCCGTTCTTCCTTCTCATAATGATAAGACAGCACTTCCTCCCCAAATTCTTTCAACCACATCACTTGCAGGAGCTGGATTCAGCTGAATGGGAGGAAGTCACTGGCCCCCCGAGACGGTCTCTCAGCGTCTCTACAAGGGAATTGGAAACTACAATCGAAGAAGGTGAAGTGGAGATGTGTGATGCTGAGATATTAGATGAGTTGACAACCAGCAGAGGGGAGCTGAAAATCAGAGCTAGCTTCAGTGAAGACAAACGTGGTCAGATTCACCCTTCAAATTTTGGCCAAACAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006820 GO:0006855 GO:0006873 GO:0006885 GO:0007154 GO:0008150 GO:0008509 GO:0009605 GO:0009987 GO:0009991 GO:0010036 GO:0015075 GO:0015103 GO:0015238 GO:0015318 GO:0015562 GO:0015698 GO:0015893 GO:0016020 GO:0016021 GO:0019725 GO:0022857 GO:0030003 GO:0030004 GO:0030641 GO:0031224 GO:0031226 GO:0031667 GO:0031668 GO:0031669 GO:0034220 GO:0035445 GO:0042221 GO:0042493 GO:0042592 GO:0044425 GO:0044459 GO:0044464 GO:0046713 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051453 GO:0051716 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0070887 GO:0071496 GO:0071944 GO:0080029 GO:0080139 GO:0098656 GO:0098660 GO:0098661 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HCO3_cotransp

Protein Analysis

1177

Amino Acids

133.35

Weight (kDa)

8.59

Isoelectric Point (pI)

43.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BAH	PF01426	38 - 152	6.2e-07	BAH domain
HCO3_cotransp	PF00955	517 - 693	2.1e-35	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	709 - 885	1.1e-25	HCO3- transporter integral membrane domain
HCO3_cotransp	PF00955	965 - 1107	8.7e-28	HCO3- transporter integral membrane domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002843)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15605 AT3G15605 AT3G15605 AT3G15605
fragaria_vesca	FvH4_6g30540 FvH4_6g30540 FvH4_6g30540 FvH4_6g30540 FvH4_6g30540 FvH4_6g30540 FvH4_6g30540 FvH4_6g30540 FvH4_6g30540 FvH4_6g30540
malus_domestica	MD09G1219000.v1.1
prunus_persica	Prupe.3G077800_v2.0.a1 Prupe.3G077800_v2.0.a1 Prupe.3G077800_v2.0.a1 Prupe.3G077800_v2.0.a1 Prupe.3G077800_v2.0.a1
pyrus_communis	pycom09g13690
rosa_chinensis	RchiOBHm_Chr2g0136961
rosa_laevigata	RLG00000019591
rosa_multiflora	Rmu_co8420089.1_g000001 Rmu_sc0001374.1_g000069
rosa_roxburghii	Rroxscaffold_2G00107840 Rroxscaffold_2G00107850
rosa_rugosa	Rorug02G0335700 Rorug02G0335800 Rorug02G0335900 Rorug02G0336000 Rorug02G0336100 Rorug02G0336200
rosa_samantha	Rh2AG385600 Rh2BG392600 Rh2CG372800 Rh2DG408700
rosa_wichuraiana	Rw2G031540 Rw2G031550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	810, 1832
Acc65I	GGTACC	1 cut(s)	1151
AccB1I	GGYRCC	4 cut(s)	914, 1151, 1471, 2614
AccB7I	CCANNNNNTGG	1 cut(s)	1046
AccI	GTMKAC	2 cut(s)	835, 3177
AccII	CGCG	1 cut(s)	1884
AccIII	TCCGGA	1 cut(s)	478
AciI	CCGC	4 cut(s)	839, 895, 2421, 2447
AclWI	GGATC	7 cut(s)	489, 935, 978, 2081, 2855, 3202, 3215
AcoI	YGGCCR	5 cut(s)	230, 417, 1483, 3018, 3520
AcsI	RAATTY	5 cut(s)	23, 180, 1424, 3281, 3514
AcuI	CTGAAG	4 cut(s)	924, 1653, 2763, 3463
AcyI	GRCGYC	1 cut(s)	673
AflII	CTTAAG	1 cut(s)	2645
AflIII	ACRYGT	1 cut(s)	1839
AjnI	CCWGG	2 cut(s)	542, 2404
AjuI	GAANNNNNNNTTGG	6 cut(s)	147, 179, 327, 359, 413, 445
AloI	GAACNNNNNNTCC	2 cut(s)	59, 91
Alw21I	GWGCWC	2 cut(s)	791, 1733
Alw26I	GTCTC	5 cut(s)	1132, 2456, 3343, 3360, 3370
AlwI	GGATC	7 cut(s)	489, 935, 978, 2081, 2855, 3202, 3215
AlwNI	CAGNNNCTG	1 cut(s)	3310
Ama87I	CYCGRG	1 cut(s)	3346
Aor13HI	TCCGGA	1 cut(s)	478
ApeKI	GCWGC	4 cut(s)	983, 1586, 2545, 2945
ApoI	RAATTY	5 cut(s)	23, 180, 1424, 3281, 3514
ArsI	GACNNNNNNTTYG	4 cut(s)	133, 165, 1915, 1947
Asp700I	GAANNNNTTC	3 cut(s)	1890, 3044, 3108
Asp718I	GGTACC	1 cut(s)	1151
AspLEI	GCGC	3 cut(s)	149, 459, 3079
AspS9I	GGNCC	8 cut(s)	550, 560, 686, 766, 922, 1372, 1642, 3341
AsuC2I	CCSGG	2 cut(s)	2897, 3036
AsuHPI	GGTGA	6 cut(s)	47, 269, 532, 2093, 3413, 3497
AsuII	TTCGAA	1 cut(s)	2662
AsuNHI	GCTAGC	2 cut(s)	2708, 3473
AvaI	CYCGRG	1 cut(s)	3346
AvaII	GGWCC	6 cut(s)	550, 560, 686, 766, 922, 1372
BaeGI	GKGCMC	1 cut(s)	1474
BaeI	ACNNNNGTAYC	2 cut(s)	2766, 2799
BalI	TGGCCA	2 cut(s)	3020, 3522
BamHI	GGATCC	1 cut(s)	3207
BanI	GGYRCC	4 cut(s)	914, 1151, 1471, 2614
BbsI	GAAGAC	4 cut(s)	2186, 2805, 3023, 3492
Bbv12I	GWGCWC	2 cut(s)	791, 1733
BbvI	GCAGC	4 cut(s)	970, 1598, 2532, 2957
BceAI	ACGGC	2 cut(s)	432, 3223
BcgI	CGANNNNNNTGC	2 cut(s)	2642, 2676
BciT130I	CCWGG	2 cut(s)	544, 2406
BciVI	GTATCC	4 cut(s)	370, 1436, 2402, 2996
BcnI	CCSGG	2 cut(s)	2897, 3036
BcoDI	GTCTC	5 cut(s)	1132, 2456, 3343, 3360, 3370
BfmI	CTRYAG	7 cut(s)	825, 1095, 1159, 1331, 1752, 2072, 2769
BfoI	RGCGCY	1 cut(s)	150
BfrI	CTTAAG	1 cut(s)	2645
BfuI	GTATCC	4 cut(s)	370, 1436, 2402, 2996
BisI	GCNGC	5 cut(s)	984, 1587, 2421, 2546, 2946
BlpI	GCTNAGC	1 cut(s)	1284
BlsI	GCNGC	5 cut(s)	985, 1588, 2422, 2547, 2947
BmcAI	AGTACT	1 cut(s)	110
Bme1390I	CCNGG	4 cut(s)	544, 2406, 2897, 3036
Bme18I	GGWCC	6 cut(s)	550, 560, 686, 766, 922, 1372
BmeT110I	CYCGRG	1 cut(s)	3346
BmgT120I	GGNCC	8 cut(s)	550, 560, 686, 766, 922, 1372, 1642, 3341
BmiI	GGNNCC	9 cut(s)	768, 916, 1153, 1473, 1644, 2250, 2616, 3209, 3343
BmrFI	CCNGG	4 cut(s)	544, 2406, 2897, 3036
BmsI	GCATC	6 cut(s)	1072, 1106, 2842, 2869, 2957, 3409
BmtI	GCTAGC	2 cut(s)	2712, 3477
BpiI	GAAGAC	4 cut(s)	2186, 2805, 3023, 3492
BplI	GAGNNNNNCTC	2 cut(s)	3340, 3372
Bpu1102I	GCTNAGC	1 cut(s)	1284
Bpu14I	TTCGAA	1 cut(s)	2662
BpuEI	CTTGAG	1 cut(s)	1747
BpuMI	CCSGG	2 cut(s)	2897, 3036
BsaAI	YACGTR	1 cut(s)	1842
BsaHI	GRCGYC	1 cut(s)	673
BsaI	GGTCTC	3 cut(s)	1132, 2456, 3360
BsaJI	CCNNGG	2 cut(s)	918, 3035
BsaWI	WCCGGW	2 cut(s)	72, 478
Bse118I	RCCGGY	2 cut(s)	1497, 3218
Bse1I	ACTGG	6 cut(s)	82, 148, 278, 611, 1325, 3343
Bse3DI	GCAATG	4 cut(s)	1320, 2232, 3072, 3147
BseAI	TCCGGA	1 cut(s)	478
BseBI	CCWGG	2 cut(s)	544, 2406
BseDI	CCNNGG	2 cut(s)	918, 3035
BseGI	GGATG	6 cut(s)	73, 552, 944, 953, 1913, 1925
BseMI	GCAATG	4 cut(s)	1320, 2232, 3072, 3147
BseMII	CTCAG	6 cut(s)	1298, 1506, 1694, 2856, 3373, 3414
BseNI	ACTGG	6 cut(s)	82, 148, 278, 611, 1325, 3343
BseSI	GKGCMC	1 cut(s)	1474
BseXI	GCAGC	4 cut(s)	970, 1598, 2532, 2957
BseYI	CCCAGC	1 cut(s)	2548
Bsh1236I	CGCG	1 cut(s)	1884
BshNI	GGYRCC	4 cut(s)	914, 1151, 1471, 2614
BsiHKAI	GWGCWC	2 cut(s)	791, 1733
BsiHKCI	CYCGRG	1 cut(s)	3346
BsiSI	CCGG	7 cut(s)	73, 479, 1482, 1498, 2896, 3035, 3219
BsiWI	CGTACG	1 cut(s)	437
BslFI	GGGAC	2 cut(s)	1180, 1893
BsmAI	GTCTC	5 cut(s)	1132, 2456, 3343, 3360, 3370
BsmBI	CGTCTC	2 cut(s)	3343, 3370
BsmFI	GGGAC	2 cut(s)	1180, 1893
BsmI	GAATGC	3 cut(s)	986, 1617, 2848
Bso31I	GGTCTC	3 cut(s)	1132, 2456, 3360
BsoBI	CYCGRG	1 cut(s)	3346
Bsp119I	TTCGAA	1 cut(s)	2662
Bsp1286I	GDGCHC	3 cut(s)	791, 1474, 1733
Bsp13I	TCCGGA	1 cut(s)	478
Bsp1407I	TGTACA	2 cut(s)	1836, 2121
Bsp143I	GATC	7 cut(s)	481, 940, 970, 2086, 2195, 2860, 3207
Bsp1720I	GCTNAGC	1 cut(s)	1284
BspACI	CCGC	4 cut(s)	839, 895, 2421, 2447
BspCNI	CTCAG	6 cut(s)	1297, 1505, 1695, 2857, 3372, 3415
BspEI	TCCGGA	1 cut(s)	478
BspFNI	CGCG	1 cut(s)	1884
BspLI	GGNNCC	9 cut(s)	768, 916, 1153, 1473, 1644, 2250, 2616, 3209, 3343
BspMAI	CTGCAG	4 cut(s)	829, 1163, 1335, 2773
BspOI	GCTAGC	2 cut(s)	2712, 3477
BspPI	GGATC	7 cut(s)	489, 935, 978, 2081, 2855, 3202, 3215
BspQI	GCTCTTC	2 cut(s)	1576, 1680
BspT104I	TTCGAA	1 cut(s)	2662
BspT107I	GGYRCC	4 cut(s)	914, 1151, 1471, 2614
BspTI	CTTAAG	1 cut(s)	2645
BspTNI	GGTCTC	3 cut(s)	1132, 2456, 3360
BsrDI	GCAATG	4 cut(s)	1320, 2232, 3072, 3147
BsrFI	RCCGGY	2 cut(s)	1497, 3218
BsrGI	TGTACA	2 cut(s)	1836, 2121
BsrI	ACTGG	6 cut(s)	82, 148, 278, 611, 1325, 3343
BssAI	RCCGGY	2 cut(s)	1497, 3218
BssECI	CCNNGG	2 cut(s)	918, 3035
BssMI	GATC	7 cut(s)	481, 940, 970, 2086, 2195, 2860, 3207
BssNAI	GTATAC	1 cut(s)	3178
BssNI	GRCGYC	1 cut(s)	673
BssT1I	CCWWGG	1 cut(s)	918
Bst1107I	GTATAC	1 cut(s)	3178
Bst2UI	CCWGG	2 cut(s)	544, 2406
Bst6I	CTCTTC	6 cut(s)	399, 1242, 1380, 1576, 1680, 3074
BstACI	GRCGYC	1 cut(s)	673
BstAFI	CTTAAG	1 cut(s)	2645
BstAPI	GCANNNNNTGC	1 cut(s)	2852
BstAUI	TGTACA	2 cut(s)	1836, 2121
BstBAI	YACGTR	1 cut(s)	1842
BstBI	TTCGAA	1 cut(s)	2662
BstDEI	CTNAG	7 cut(s)	1284, 1492, 1703, 2628, 2865, 3359, 3423
BstENI	CCTNNNNNAGG	1 cut(s)	2609
BstF5I	GGATG	6 cut(s)	73, 552, 944, 953, 1913, 1925
BstFNI	CGCG	1 cut(s)	1884
BstH2I	RGCGCY	1 cut(s)	150
BstHHI	GCGC	3 cut(s)	149, 459, 3079
BstKTI	GATC	7 cut(s)	484, 943, 973, 2089, 2198, 2863, 3210
BstMAI	GTCTC	5 cut(s)	1132, 2456, 3343, 3360, 3370
BstMBI	GATC	7 cut(s)	481, 940, 970, 2086, 2195, 2860, 3207
BstMWI	GCNNNNNNNGC	6 cut(s)	529, 1091, 1754, 2852, 2966, 3017
BstNI	CCWGG	2 cut(s)	544, 2406
BstNSI	RCATGY	2 cut(s)	1593, 2014
BstSCI	CCNGG	4 cut(s)	542, 2404, 2895, 3034
BstSFI	CTRYAG	7 cut(s)	825, 1095, 1159, 1331, 1752, 2072, 2769
BstSLI	GKGCMC	1 cut(s)	1474
BstUI	CGCG	1 cut(s)	1884
BstV1I	GCAGC	4 cut(s)	970, 1598, 2532, 2957
BstV2I	GAAGAC	4 cut(s)	2186, 2805, 3023, 3492
BstX2I	RGATCY	4 cut(s)	940, 970, 2086, 3207
BstXI	CCANNNNNNTGG	1 cut(s)	879
BstYI	RGATCY	4 cut(s)	940, 970, 2086, 3207
BstZ17I	GTATAC	1 cut(s)	3178
BsuI	GTATCC	4 cut(s)	370, 1436, 2402, 2996
BtsCI	GGATG	6 cut(s)	73, 552, 944, 953, 1913, 1925
BtsI	GCAGTG	1 cut(s)	2561
BtsIMutI	CAGTG	8 cut(s)	271, 768, 2293, 2476, 2561, 3138, 3336, 3487
CaiI	CAGNNNCTG	1 cut(s)	3310
CfoI	GCGC	3 cut(s)	149, 459, 3079
Cfr10I	RCCGGY	2 cut(s)	1497, 3218
Cfr13I	GGNCC	8 cut(s)	550, 560, 686, 766, 922, 1372, 1642, 3341
CseI	GACGC	4 cut(s)	662, 1890, 1906, 3352
CspCI	CAANNNNNGTGG	2 cut(s)	3282, 3317
DdeI	CTNAG	7 cut(s)	1284, 1492, 1703, 2628, 2865, 3359, 3423
DpnI	GATC	7 cut(s)	483, 942, 972, 2088, 2197, 2862, 3209
DpnII	GATC	7 cut(s)	481, 940, 970, 2086, 2195, 2860, 3207
EaeI	YGGCCR	5 cut(s)	230, 417, 1483, 3018, 3520
Eam1104I	CTCTTC	6 cut(s)	399, 1242, 1380, 1576, 1680, 3074
EarI	CTCTTC	6 cut(s)	399, 1242, 1380, 1576, 1680, 3074
Eco130I	CCWWGG	1 cut(s)	918
Eco147I	AGGCCT	1 cut(s)	312
Eco31I	GGTCTC	3 cut(s)	1132, 2456, 3360
Eco47I	GGWCC	6 cut(s)	550, 560, 686, 766, 922, 1372
Eco57I	CTGAAG	4 cut(s)	924, 1653, 2763, 3463
Eco88I	CYCGRG	1 cut(s)	3346
EcoNI	CCTNNNNNAGG	1 cut(s)	2609
EcoRII	CCWGG	2 cut(s)	542, 2404
EcoT14I	CCWWGG	1 cut(s)	918
EcoT22I	ATGCAT	1 cut(s)	2857
ErhI	CCWWGG	1 cut(s)	918
Esp3I	CGTCTC	2 cut(s)	3343, 3370
FalI	AAGNNNNNCTT	2 cut(s)	685, 717
FaqI	GGGAC	2 cut(s)	1180, 1893
FauI	CCCGC	1 cut(s)	2440
FblI	GTMKAC	2 cut(s)	835, 3177
Fnu4HI	GCNGC	5 cut(s)	984, 1587, 2421, 2546, 2946
FokI	GGATG	6 cut(s)	80, 559, 931, 940, 1912, 1920
Fsp4HI	GCNGC	5 cut(s)	984, 1587, 2421, 2546, 2946
GlaI	GCGC	3 cut(s)	148, 458, 3078
GluI	GCNGC	5 cut(s)	984, 1587, 2421, 2546, 2946
GsaI	CCCAGC	1 cut(s)	2552
HaeII	RGCGCY	1 cut(s)	150
HapII	CCGG	7 cut(s)	73, 479, 1482, 1498, 2896, 3035, 3219
HgaI	GACGC	4 cut(s)	662, 1890, 1906, 3352
HhaI	GCGC	3 cut(s)	149, 459, 3079
Hin1I	GRCGYC	1 cut(s)	673
Hin6I	GCGC	3 cut(s)	147, 457, 3077
HinP1I	GCGC	3 cut(s)	147, 457, 3077
HincII	GTYRAC	3 cut(s)	451, 2902, 3441
HindII	GTYRAC	3 cut(s)	451, 2902, 3441
HindIII	AAGCTT	1 cut(s)	1723
HpaI	GTTAAC	1 cut(s)	2902
HpaII	CCGG	7 cut(s)	73, 479, 1482, 1498, 2896, 3035, 3219
HphI	GGTGA	6 cut(s)	47, 269, 532, 2093, 3413, 3497
Hpy99I	CGWCG	3 cut(s)	438, 1276, 3090
HpyCH4IV	ACGT	8 cut(s)	440, 447, 1243, 1271, 1841, 2110, 3088, 3493
HpyF10VI	GCNNNNNNNGC	6 cut(s)	529, 1091, 1754, 2852, 2966, 3017
HpyF3I	CTNAG	7 cut(s)	1284, 1492, 1703, 2628, 2865, 3359, 3423
HpySE526I	ACGT	8 cut(s)	440, 447, 1243, 1271, 1841, 2110, 3088, 3493
Hsp92I	GRCGYC	1 cut(s)	673
HspAI	GCGC	3 cut(s)	147, 457, 3077
Kpn2I	TCCGGA	1 cut(s)	478
KpnI	GGTACC	1 cut(s)	1155
KroI	GCCGGC	1 cut(s)	3218
KroNI	GCCGGC	1 cut(s)	3220
KspAI	GTTAAC	1 cut(s)	2902
Kzo9I	GATC	7 cut(s)	481, 940, 970, 2086, 2195, 2860, 3207
LguI	GCTCTTC	2 cut(s)	1576, 1680
LmnI	GCTCC	3 cut(s)	1696, 3307, 3457
Lsp1109I	GCAGC	4 cut(s)	970, 1598, 2532, 2957
LweI	GCATC	6 cut(s)	1072, 1106, 2842, 2869, 2957, 3409
MaeII	ACGT	8 cut(s)	440, 447, 1243, 1271, 1841, 2110, 3088, 3493
MaeIII	GTNAC	8 cut(s)	392, 1500, 1964, 2785, 2931, 3089, 3199, 3334
MalI	GATC	7 cut(s)	483, 942, 972, 2088, 2197, 2862, 3209
MboI	GATC	7 cut(s)	481, 940, 970, 2086, 2195, 2860, 3207
MfeI	CAATTG	2 cut(s)	339, 587
MflI	RGATCY	4 cut(s)	940, 970, 2086, 3207
MhlI	GDGCHC	3 cut(s)	791, 1474, 1733
MlsI	TGGCCA	2 cut(s)	3020, 3522
MluNI	TGGCCA	2 cut(s)	3020, 3522
MlyI	GAGTC	5 cut(s)	676, 1117, 1162, 2123, 3137
MmeI	TCCRAC	2 cut(s)	325, 1885
Mox20I	TGGCCA	2 cut(s)	3020, 3522
Mph1103I	ATGCAT	1 cut(s)	2857
MroI	TCCGGA	1 cut(s)	478
MroNI	GCCGGC	1 cut(s)	3218
MroXI	GAANNNNTTC	3 cut(s)	1890, 3044, 3108
MscI	TGGCCA	2 cut(s)	3020, 3522
MslI	CAYNNNNRTG	3 cut(s)	545, 2753, 2877
Msp20I	TGGCCA	2 cut(s)	3020, 3522
MspA1I	CMGCKG	3 cut(s)	236, 902, 3320
MspCI	CTTAAG	1 cut(s)	2645
MspI	CCGG	7 cut(s)	73, 479, 1482, 1498, 2896, 3035, 3219
MspR9I	CCNGG	4 cut(s)	544, 2406, 2897, 3036
MunI	CAATTG	2 cut(s)	339, 587
Mva1269I	GAATGC	3 cut(s)	986, 1617, 2848
MvaI	CCWGG	2 cut(s)	544, 2406
MvnI	CGCG	1 cut(s)	1884
MwoI	GCNNNNNNNGC	6 cut(s)	529, 1091, 1754, 2852, 2966, 3017
NaeI	GCCGGC	1 cut(s)	3220
NciI	CCSGG	2 cut(s)	2897, 3036
NdeII	GATC	7 cut(s)	481, 940, 970, 2086, 2195, 2860, 3207
NgoMIV	GCCGGC	1 cut(s)	3218
NheI	GCTAGC	2 cut(s)	2708, 3473
NlaIV	GGNNCC	9 cut(s)	768, 916, 1153, 1473, 1644, 2250, 2616, 3209, 3343
NmuCI	GTSAC	6 cut(s)	392, 1500, 1964, 2931, 3199, 3334
NsiI	ATGCAT	1 cut(s)	2857
NspI	RCATGY	2 cut(s)	1593, 2014
NspV	TTCGAA	1 cut(s)	2662
PaeI	GCATGC	1 cut(s)	1593
PceI	AGGCCT	1 cut(s)	312
PciSI	GCTCTTC	2 cut(s)	1576, 1680
PctI	GAATGC	3 cut(s)	986, 1617, 2848
PdiI	GCCGGC	1 cut(s)	3220
PdmI	GAANNNNTTC	3 cut(s)	1890, 3044, 3108
Pfl23II	CGTACG	1 cut(s)	437
PflMI	CCANNNNNTGG	1 cut(s)	1046
PfoI	TCCNGGA	1 cut(s)	2404
PkrI	GCNGC	5 cut(s)	985, 1588, 2422, 2547, 2947
PleI	GAGTC	5 cut(s)	675, 1117, 1162, 2122, 3136
PpsI	GAGTC	5 cut(s)	675, 1117, 1162, 2122, 3136
Ppu21I	YACGTR	1 cut(s)	1842
PsiI	TTATAA	2 cut(s)	810, 1832
Psp6I	CCWGG	2 cut(s)	542, 2404
PspFI	CCCAGC	1 cut(s)	2548
PspGI	CCWGG	2 cut(s)	542, 2404
PspLI	CGTACG	1 cut(s)	437
PspN4I	GGNNCC	9 cut(s)	768, 916, 1153, 1473, 1644, 2250, 2616, 3209, 3343
PspPI	GGNCC	8 cut(s)	550, 560, 686, 766, 922, 1372, 1642, 3341
PstI	CTGCAG	4 cut(s)	829, 1163, 1335, 2773
PstNI	CAGNNNCTG	1 cut(s)	3310
PsuI	RGATCY	4 cut(s)	940, 970, 2086, 3207
PvuII	CAGCTG	3 cut(s)	236, 902, 3320
RseI	CAYNNNNRTG	3 cut(s)	545, 2753, 2877
SapI	GCTCTTC	2 cut(s)	1576, 1680
SatI	GCNGC	5 cut(s)	984, 1587, 2421, 2546, 2946
Sau3AI	GATC	7 cut(s)	481, 940, 970, 2086, 2195, 2860, 3207
Sau96I	GGNCC	8 cut(s)	550, 560, 686, 766, 922, 1372, 1642, 3341
ScaI	AGTACT	1 cut(s)	110
SchI	GAGTC	5 cut(s)	676, 1117, 1162, 2123, 3137
ScrFI	CCNGG	4 cut(s)	544, 2406, 2897, 3036
SduI	GDGCHC	3 cut(s)	791, 1474, 1733
SfaNI	GCATC	6 cut(s)	1072, 1106, 2842, 2869, 2957, 3409
SfcI	CTRYAG	7 cut(s)	825, 1095, 1159, 1331, 1752, 2072, 2769
SfuI	TTCGAA	1 cut(s)	2662
SinI	GGWCC	6 cut(s)	550, 560, 686, 766, 922, 1372
SmiMI	CAYNNNNRTG	3 cut(s)	545, 2753, 2877
SmlI	CTYRAG	2 cut(s)	1726, 2645
SmoI	CTYRAG	2 cut(s)	1726, 2645
SphI	GCATGC	1 cut(s)	1593
SseBI	AGGCCT	1 cut(s)	312
SsiI	CCGC	4 cut(s)	839, 895, 2421, 2447
SspI	AATATT	4 cut(s)	465, 512, 1953, 2302
StuI	AGGCCT	1 cut(s)	312
StyD4I	CCNGG	4 cut(s)	542, 2404, 2895, 3034
StyI	CCWWGG	1 cut(s)	918
TaiI	ACGT	8 cut(s)	443, 450, 1246, 1274, 1844, 2113, 3091, 3496
TaqI	TCGA	4 cut(s)	1576, 2662, 2765, 3393
TatI	WGTACW	5 cut(s)	108, 964, 1456, 1836, 2121
TauI	GCSGC	1 cut(s)	2423
TscAI	CASTG	8 cut(s)	278, 768, 2293, 2476, 2568, 3138, 3343, 3487
TseFI	GTSAC	6 cut(s)	392, 1500, 1964, 2931, 3199, 3334
TseI	GCWGC	4 cut(s)	983, 1586, 2545, 2945
Tsp45I	GTSAC	6 cut(s)	392, 1500, 1964, 2931, 3199, 3334
TspGWI	ACGGA	3 cut(s)	115, 422, 635
TspRI	CASTG	8 cut(s)	278, 768, 2293, 2476, 2568, 3138, 3343, 3487
Van91I	CCANNNNNTGG	1 cut(s)	1046
Vha464I	CTTAAG	1 cut(s)	2645
VpaK11BI	GGWCC	6 cut(s)	550, 560, 686, 766, 922, 1372
XagI	CCTNNNNNAGG	1 cut(s)	2609
XapI	RAATTY	5 cut(s)	23, 180, 1424, 3281, 3514
XbaI	TCTAGA	2 cut(s)	676, 937
XceI	RCATGY	2 cut(s)	1593, 2014
XcmI	CCANNNNNNNNNTGG	1 cut(s)	586
XmiI	GTMKAC	2 cut(s)	835, 3177
XmnI	GAANNNNTTC	3 cut(s)	1890, 3044, 3108
ZrmI	AGTACT	1 cut(s)	110
Zsp2I	ATGCAT	1 cut(s)	2857

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.