Rh4BG205400

Belongs to the universal ribosomal protein uS9 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001512
Physical Location & Seq
Forward (+)
35324178 .. 35324897
720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG205400.1

Sequence Viewer

Length: 438 bp
ATGGCGGCTCCCCCGATCAAATCGGTCCAGTGCTTCGGGCGCAAGAAGACGGCGGTGGCGGTCACCTACTGCAAGGAAGGCCGCGGCTTGATCAAGATCAACGGCTGCCCAATCGAGCTCGTGGAGCCGGAGATCCTCCGCTTCAAGGCCTACGAGCCCATCCTTCTGCTCGGACGACACCGTTTCGCCGGCGTCGACATGAGGATCCGCGTCAAGGGAGGAGGTCACACTTCTCAGATCTACGCCATCCGTCAGAGCATCGCCAAGGCCTTGGTCGCTTATTACCAGAAGTTTGTGGATGAGCAGAGCAAGAAGGAAATCAAGGACATTCTCGTCAGGTACGACCGTACTCTCCTCGTCGCCGATCCAAGGCGCTGCGAGCCCAAGAAGTTTGGTGGTCGTGGTGCCAGAGCTAGGTTCCAGAAGTCTTACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S9

Protein Analysis

145

Amino Acids

16.51

Weight (kDa)

10.16

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S9 PF00380 13 - 145 4.3e-38 Ribosomal protein S9/S16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 332
AccB1I GGYRCC 1 cut(s) 404
AccI GTMKAC 1 cut(s) 195
AccII CGCG 2 cut(s) 84, 210
AciI CCGC 7 cut(s) 5, 53, 59, 82, 84, 139, 208
AclWI GGATC 4 cut(s) 127, 199, 212, 359
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 2 cut(s) 341, 349
AfiI CCNNNNNNNGG 4 cut(s) 145, 214, 369, 414
AgsI TTSAA 1 cut(s) 145
AluBI AGCT 2 cut(s) 118, 413
AluI AGCT 2 cut(s) 118, 413
Alw21I GWGCWC 1 cut(s) 120
AlwI GGATC 4 cut(s) 127, 199, 212, 359
AoxI GGCC 3 cut(s) 79, 147, 267
ApeKI GCWGC 2 cut(s) 105, 375
AspLEI GCGC 2 cut(s) 42, 375
AspS9I GGNCC 1 cut(s) 25
AsuHPI GGTGA 1 cut(s) 55
AvaII GGWCC 1 cut(s) 25
BaeI ACNNNNGTAYC 2 cut(s) 331, 364
BamHI GGATCC 1 cut(s) 204
BanI GGYRCC 1 cut(s) 404
BanII GRGCYC 3 cut(s) 120, 159, 384
BauI CACGAG 1 cut(s) 119
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 92, 362
BccI CCATC 2 cut(s) 167, 254
BceAI ACGGC 2 cut(s) 66, 118
BclI TGATCA 1 cut(s) 90
BfaI CTAG 1 cut(s) 414
BfoI RGCGCY 1 cut(s) 376
BglII AGATCT 1 cut(s) 237
BisI GCNGC 5 cut(s) 6, 82, 85, 106, 376
BlsI GCNGC 5 cut(s) 7, 83, 86, 107, 377
Bme18I GGWCC 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 5 cut(s) 9, 126, 206, 406, 419
BmsI GCATC 1 cut(s) 267
BpiI GAAGAC 1 cut(s) 53
BsaBI GATNNNNATC 1 cut(s) 95
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 4 cut(s) 82, 264, 270, 368
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 214, 369, 414
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 1 cut(s) 28
Bse8I GATNNNNATC 1 cut(s) 95
BseDI CCNNGG 4 cut(s) 82, 264, 270, 368
BseGI GGATG 3 cut(s) 159, 246, 304
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 4 cut(s) 145, 214, 369, 414
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 2 cut(s) 234, 344
BseXI GCAGC 2 cut(s) 92, 362
Bsh1236I CGCG 2 cut(s) 84, 210
Bsh1285I CGRYCG 1 cut(s) 346
BshFI GGCC 3 cut(s) 81, 149, 269
BshNI GGYRCC 1 cut(s) 404
BsiEI CGRYCG 1 cut(s) 346
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 2 cut(s) 128, 189
BslI CCNNNNNNNGG 4 cut(s) 145, 214, 369, 414
BsnI GGCC 3 cut(s) 81, 149, 269
Bsp1286I GDGCHC 3 cut(s) 120, 159, 384
Bsp143I GATC 7 cut(s) 15, 90, 96, 132, 204, 237, 364
BspACI CCGC 7 cut(s) 5, 53, 59, 82, 84, 139, 208
BspANI GGCC 3 cut(s) 81, 149, 269
BspCNI CTCAG 1 cut(s) 247
BspFNI CGCG 2 cut(s) 84, 210
BspLI GGNNCC 5 cut(s) 9, 126, 206, 406, 419
BspPI GGATC 4 cut(s) 127, 199, 212, 359
BspT107I GGYRCC 1 cut(s) 404
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 1 cut(s) 28
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 4 cut(s) 82, 264, 270, 368
BssMI GATC 7 cut(s) 15, 90, 96, 132, 204, 237, 364
BssNI GRCGYC 1 cut(s) 192
BssSI CACGAG 1 cut(s) 119
BssT1I CCWWGG 3 cut(s) 264, 270, 368
Bst2BI CACGAG 1 cut(s) 119
Bst4CI ACNGT 3 cut(s) 182, 347, 434
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 190, 380
BstDEI CTNAG 1 cut(s) 234
BstDSI CCRYGG 1 cut(s) 82
BstEII GGTNACC 1 cut(s) 61
BstF5I GGATG 3 cut(s) 159, 246, 304
BstFNI CGCG 2 cut(s) 84, 210
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 2 cut(s) 42, 375
BstKTI GATC 7 cut(s) 18, 93, 99, 135, 207, 240, 367
BstMBI GATC 7 cut(s) 15, 90, 96, 132, 204, 237, 364
BstMCI CGRYCG 1 cut(s) 346
BstMWI GCNNNNNNNGC 5 cut(s) 39, 78, 124, 275, 379
BstPI GGTNACC 1 cut(s) 61
BstUI CGCG 2 cut(s) 84, 210
BstV1I GCAGC 2 cut(s) 92, 362
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 3 cut(s) 132, 204, 237
BstXI CCANNNNNNTGG 1 cut(s) 271
BstYI RGATCY 3 cut(s) 132, 204, 237
BsuRI GGCC 3 cut(s) 81, 149, 269
BtgI CCRYGG 1 cut(s) 82
BtgZI GCGATG 1 cut(s) 244
BtsCI GGATG 3 cut(s) 159, 246, 304
BtsIMutI CAGTG 1 cut(s) 35
Cac8I GCNNGC 2 cut(s) 190, 380
CfoI GCGC 2 cut(s) 42, 375
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 25
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 2 cut(s) 181, 199
Csp6I GTAC 2 cut(s) 340, 348
CviAII CATG 1 cut(s) 199
CviQI GTAC 2 cut(s) 340, 348
DdeI CTNAG 1 cut(s) 234
DpnI GATC 7 cut(s) 17, 92, 98, 134, 206, 239, 366
DpnII GATC 7 cut(s) 15, 90, 96, 132, 204, 237, 364
DrdI GACNNNNNNGTC 1 cut(s) 332
DseDI GACNNNNNNGTC 1 cut(s) 332
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 3 cut(s) 264, 270, 368
Eco147I AGGCCT 2 cut(s) 149, 269
Eco24I GRGCYC 3 cut(s) 120, 159, 384
Eco47I GGWCC 1 cut(s) 25
Eco53kI GAGCTC 1 cut(s) 118
Eco91I GGTNACC 1 cut(s) 61
EcoICRI GAGCTC 1 cut(s) 118
EcoO65I GGTNACC 1 cut(s) 61
EcoT14I CCWWGG 3 cut(s) 264, 270, 368
EcoT38I GRGCYC 3 cut(s) 120, 159, 384
ErhI CCWWGG 3 cut(s) 264, 270, 368
FaeI CATG 1 cut(s) 202
FaiI YATR 1 cut(s) 200
FatI CATG 1 cut(s) 198
FbaI TGATCA 1 cut(s) 90
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 5 cut(s) 6, 82, 85, 106, 376
FokI GGATG 3 cut(s) 146, 233, 311
FriOI GRGCYC 3 cut(s) 120, 159, 384
Fsp4HI GCNGC 5 cut(s) 6, 82, 85, 106, 376
FspBI CTAG 1 cut(s) 414
GlaI GCGC 2 cut(s) 41, 374
GluI GCNGC 5 cut(s) 6, 82, 85, 106, 376
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 3 cut(s) 81, 149, 269
HapII CCGG 2 cut(s) 128, 189
HgaI GACGC 2 cut(s) 181, 199
HhaI GCGC 2 cut(s) 42, 375
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 1 cut(s) 202
Hin6I GCGC 2 cut(s) 40, 373
HinP1I GCGC 2 cut(s) 40, 373
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HpaII CCGG 2 cut(s) 128, 189
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 1 cut(s) 196
Hpy188I TCNGA 3 cut(s) 173, 237, 255
Hpy188III TCNNGA 2 cut(s) 94, 421
Hpy8I GTNNAC 1 cut(s) 196
Hpy99I CGWCG 2 cut(s) 197, 362
HpyAV CCTTC 3 cut(s) 71, 173, 307
HpyCH4III ACNGT 3 cut(s) 182, 347, 434
HpyCH4V TGCA 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 5 cut(s) 39, 78, 124, 275, 379
HpyF3I CTNAG 1 cut(s) 234
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 1 cut(s) 202
HspAI GCGC 2 cut(s) 40, 373
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Ksp22I TGATCA 1 cut(s) 90
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 7 cut(s) 15, 90, 96, 132, 204, 237, 364
LmnI GCTCC 2 cut(s) 13, 124
LpnPI CCDG 7 cut(s) 41, 141, 202, 299, 322, 421, 434
Lsp1109I GCAGC 2 cut(s) 92, 362
LweI GCATC 1 cut(s) 267
MaeI CTAG 1 cut(s) 414
MaeIII GTNAC 2 cut(s) 61, 224
MalI GATC 7 cut(s) 17, 92, 98, 134, 206, 239, 366
MboI GATC 7 cut(s) 15, 90, 96, 132, 204, 237, 364
MboII GAAGA 1 cut(s) 58
MflI RGATCY 3 cut(s) 132, 204, 237
MhlI GDGCHC 3 cut(s) 120, 159, 384
MnlI CCTC 5 cut(s) 146, 195, 212, 215, 365
MreI CGCCGGCG 1 cut(s) 188
MroNI GCCGGC 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 84
MspI CCGG 2 cut(s) 128, 189
MvnI CGCG 2 cut(s) 84, 210
MwoI GCNNNNNNNGC 5 cut(s) 39, 78, 124, 275, 379
NaeI GCCGGC 1 cut(s) 190
NdeII GATC 7 cut(s) 15, 90, 96, 132, 204, 237, 364
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 1 cut(s) 202
NlaIV GGNNCC 5 cut(s) 9, 126, 206, 406, 419
NmuCI GTSAC 2 cut(s) 61, 224
PceI AGGCCT 2 cut(s) 149, 269
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 339
PdiI GCCGGC 1 cut(s) 190
PkrI GCNGC 5 cut(s) 7, 83, 86, 107, 377
Psp124BI GAGCTC 1 cut(s) 120
PspEI GGTNACC 1 cut(s) 61
PspN4I GGNNCC 5 cut(s) 9, 126, 206, 406, 419
PspPI GGNCC 1 cut(s) 25
PsuI RGATCY 3 cut(s) 132, 204, 237
RsaI GTAC 2 cut(s) 341, 349
RsaNI GTAC 2 cut(s) 340, 348
SacI GAGCTC 1 cut(s) 120
SacII CCGCGG 1 cut(s) 85
SalI GTCGAC 1 cut(s) 194
SatI GCNGC 5 cut(s) 6, 82, 85, 106, 376
Sau3AI GATC 7 cut(s) 15, 90, 96, 132, 204, 237, 364
Sau96I GGNCC 1 cut(s) 25
SduI GDGCHC 3 cut(s) 120, 159, 384
SetI ASST 6 cut(s) 68, 120, 226, 341, 415, 419
SfaNI GCATC 1 cut(s) 267
Sfr303I CCGCGG 1 cut(s) 85
SgrAI CRCCGGYG 1 cut(s) 188
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 1 cut(s) 25
SseBI AGGCCT 2 cut(s) 149, 269
SsiI CCGC 7 cut(s) 5, 53, 59, 82, 84, 139, 208
SspMI CTAG 1 cut(s) 414
SstI GAGCTC 1 cut(s) 120
StuI AGGCCT 2 cut(s) 149, 269
StyI CCWWGG 3 cut(s) 264, 270, 368
TaaI ACNGT 3 cut(s) 182, 347, 434
TaqI TCGA 2 cut(s) 114, 195
TaqII GACCGA 1 cut(s) 13
TauI GCSGC 3 cut(s) 8, 84, 87
TscAI CASTG 1 cut(s) 35
TseFI GTSAC 2 cut(s) 61, 224
TseI GCWGC 2 cut(s) 105, 375
Tsp45I GTSAC 2 cut(s) 61, 224
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 25
XmiI GTMKAC 1 cut(s) 195
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.