Rh4DG044000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002030
Physical Location & Seq
Forward (+)
7151929 .. 7154493
2565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG044000.1

Sequence Viewer

Length: 597 bp
ATGGAGGCTTCAGAAGCACAGTATGCTGAATTTGAGGAGAAGGTCAAAAGAACTGTTTACCTTGACAACCTCTCACCACAGGTCAATGAATCGATTGTGAAACAAGCTCTGGATCAGTTTGGGAATGTGAAAAGTGTTCAATTTATTCCAAATTACGTGGAATCAAAGAACGTCCCACAGTGTGCATTGATTGAGATGGAGAACCCAACAGAGGTCAATGCCATTGTCTCAGATATAGCGCAGAAGCCTTTCATGATTGGGGGGATGCCGAGGCCTGTGAGGGCACGCAAGGCTAAAATGGAGATGTTTGACGATCGCCCTGTAAAGCCCGGTAGAACAATACAGTTTCGTTGGTTGGAGCCAAATGATCCTGATTTTGAGGTGGCAAAGAAACTGAAGGAGCTTGCTAAAATTCATGCTGTGGAAGCTGAATATGTACTCAAGAAACAACTGGAGGATGAAGAGAAACTTGAAAAACAGCATCAAGAATCCCTCAAAGCAACACATAAGAAGTATAAAATGATAGATAGTGTGATAGCTGATGGAACTACTAGGCAATTGGCCCGCGGTTATCATCTTCGGGTTGCAGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

198

Amino Acids

22.79

Weight (kDa)

6.15

Isoelectric Point (pI)

43.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 19 - 77 4.6e-06 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 567
AciI CCGC 2 cut(s) 565, 567
AclWI GGATC 2 cut(s) 120, 362
AcsI RAATTY 2 cut(s) 29, 411
AcuI CTGAAG 1 cut(s) 416
AdeI CACNNNGTG 1 cut(s) 182
AfaI GTAC 1 cut(s) 438
AfiI CCNNNNNNNGG 1 cut(s) 211
AgsI TTSAA 2 cut(s) 140, 473
AluBI AGCT 4 cut(s) 107, 403, 428, 539
AluI AGCT 4 cut(s) 107, 403, 428, 539
Alw26I GTCTC 1 cut(s) 232
AlwI GGATC 2 cut(s) 120, 362
AoxI GGCC 2 cut(s) 272, 561
ApoI RAATTY 2 cut(s) 29, 411
Asp700I GAANNNNTTC 1 cut(s) 248
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 562
AsuC2I CCSGG 1 cut(s) 330
AsuHPI GGTGA 1 cut(s) 66
BaeGI GKGCMC 1 cut(s) 286
BccI CCATC 2 cut(s) 190, 536
BcnI CCSGG 1 cut(s) 330
BcoDI GTCTC 1 cut(s) 232
BfaI CTAG 1 cut(s) 552
Bme1390I CCNGG 1 cut(s) 330
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 1 cut(s) 330
BmsI GCATC 2 cut(s) 255, 490
BpmI CTGGAG 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 330
Bsa29I ATCGAT 1 cut(s) 92
BsaAI YACGTR 1 cut(s) 157
BsaJI CCNNGG 2 cut(s) 269, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 456
BseCI ATCGAT 1 cut(s) 92
BseDI CCNNGG 2 cut(s) 269, 565
BseGI GGATG 2 cut(s) 270, 463
BseLI CCNNNNNNNGG 1 cut(s) 211
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 1 cut(s) 456
BseRI GAGGAG 1 cut(s) 50
BseSI GKGCMC 1 cut(s) 286
Bsh1236I CGCG 1 cut(s) 567
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 2 cut(s) 274, 563
BshVI ATCGAT 1 cut(s) 92
BsiEI CGRYCG 1 cut(s) 316
BsiSI CCGG 1 cut(s) 330
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 158
BsnI GGCC 2 cut(s) 274, 563
Bsp1286I GDGCHC 1 cut(s) 286
Bsp143I GATC 3 cut(s) 112, 313, 367
BspACI CCGC 2 cut(s) 565, 567
BspANI GGCC 2 cut(s) 274, 563
BspCNI CTCAG 1 cut(s) 242
BspDI ATCGAT 1 cut(s) 92
BspFNI CGCG 1 cut(s) 567
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 1 cut(s) 360
BspPI GGATC 2 cut(s) 120, 362
BsrI ACTGG 1 cut(s) 456
BssECI CCNNGG 2 cut(s) 269, 565
BssMI GATC 3 cut(s) 112, 313, 367
Bst4CI ACNGT 4 cut(s) 21, 55, 180, 345
Bst6I CTCTTC 1 cut(s) 456
BstAPI GCANNNNNTGC 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 157
BstC8I GCNNGC 3 cut(s) 286, 405, 565
BstDEI CTNAG 1 cut(s) 229
BstDSI CCRYGG 1 cut(s) 565
BstF5I GGATG 2 cut(s) 270, 463
BstFNI CGCG 1 cut(s) 567
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 3 cut(s) 115, 316, 370
BstMAI GTCTC 1 cut(s) 232
BstMBI GATC 3 cut(s) 112, 313, 367
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 4 cut(s) 14, 23, 290, 425
BstSCI CCNGG 1 cut(s) 328
BstSLI GKGCMC 1 cut(s) 286
BstUI CGCG 1 cut(s) 567
Bsu15I ATCGAT 1 cut(s) 92
BsuRI GGCC 2 cut(s) 274, 563
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 565
BtsCI GGATG 2 cut(s) 270, 463
BtsIMutI CAGTG 1 cut(s) 185
Cac8I GCNNGC 3 cut(s) 286, 405, 565
CciI TCATGA 1 cut(s) 252
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 1 cut(s) 562
Cfr42I CCGCGG 1 cut(s) 568
ClaI ATCGAT 1 cut(s) 92
Csp6I GTAC 1 cut(s) 437
CspCI CAANNNNNGTGG 2 cut(s) 138, 173
CviAII CATG 2 cut(s) 253, 416
CviQI GTAC 1 cut(s) 437
DdeI CTNAG 1 cut(s) 229
DpnI GATC 3 cut(s) 114, 315, 369
DpnII GATC 3 cut(s) 112, 313, 367
DraIII CACNNNGTG 1 cut(s) 182
Eam1104I CTCTTC 1 cut(s) 456
EarI CTCTTC 1 cut(s) 456
Eco147I AGGCCT 1 cut(s) 274
Eco57I CTGAAG 1 cut(s) 416
FaeI CATG 2 cut(s) 256, 419
FaiI YATR 7 cut(s) 24, 236, 254, 417, 435, 507, 516
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FaqI GGGAC 1 cut(s) 158
FatI CATG 2 cut(s) 252, 415
FauI CCCGC 1 cut(s) 572
FokI GGATG 2 cut(s) 277, 470
FspBI CTAG 1 cut(s) 552
GlaI GCGC 1 cut(s) 240
GsuI CTGGAG 1 cut(s) 473
HaeIII GGCC 2 cut(s) 274, 563
HapII CCGG 1 cut(s) 330
HhaI GCGC 1 cut(s) 241
Hin1II CATG 2 cut(s) 256, 419
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HinfI GANTC 3 cut(s) 89, 161, 488
HpaII CCGG 1 cut(s) 330
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 2 cut(s) 13, 232
Hpy188III TCNNGA 5 cut(s) 110, 253, 371, 442, 485
Hpy8I GTNNAC 1 cut(s) 58
HpyAV CCTTC 2 cut(s) 34, 391
HpyCH4III ACNGT 4 cut(s) 21, 55, 180, 345
HpyCH4IV ACGT 2 cut(s) 156, 171
HpyCH4V TGCA 2 cut(s) 185, 587
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 23, 290, 425
HpyF3I CTNAG 1 cut(s) 229
HpySE526I ACGT 2 cut(s) 156, 171
Hsp92II CATG 2 cut(s) 256, 419
HspAI GCGC 1 cut(s) 239
KspI CCGCGG 1 cut(s) 568
Kzo9I GATC 3 cut(s) 112, 313, 367
LmnI GCTCC 2 cut(s) 358, 400
LpnPI CCDG 7 cut(s) 65, 95, 288, 333, 343, 384, 437
LweI GCATC 2 cut(s) 255, 490
MaeI CTAG 1 cut(s) 552
MaeII ACGT 2 cut(s) 156, 171
MalI GATC 3 cut(s) 114, 315, 369
MboI GATC 3 cut(s) 112, 313, 367
MboII GAAGA 2 cut(s) 473, 569
MfeI CAATTG 1 cut(s) 557
MhlI GDGCHC 1 cut(s) 286
MluCI AATT 5 cut(s) 29, 140, 151, 411, 557
MmeI TCCRAC 1 cut(s) 336
MnlI CCTC 8 cut(s) 28, 80, 205, 264, 273, 373, 448, 503
MroXI GAANNNNTTC 1 cut(s) 248
MspA1I CMGCKG 1 cut(s) 567
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 1 cut(s) 330
MunI CAATTG 1 cut(s) 557
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 4 cut(s) 14, 23, 290, 425
NciI CCSGG 1 cut(s) 330
NdeII GATC 3 cut(s) 112, 313, 367
NlaIII CATG 2 cut(s) 256, 419
NlaIV GGNNCC 1 cut(s) 360
NmeAIII GCCGAG 1 cut(s) 294
PagI TCATGA 1 cut(s) 252
PceI AGGCCT 1 cut(s) 274
PdmI GAANNNNTTC 1 cut(s) 248
PfeI GAWTC 3 cut(s) 89, 161, 488
Ple19I CGATCG 1 cut(s) 316
Ppu21I YACGTR 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 1 cut(s) 562
PvuI CGATCG 1 cut(s) 316
RsaI GTAC 1 cut(s) 438
RsaNI GTAC 1 cut(s) 437
SacII CCGCGG 1 cut(s) 568
Sau3AI GATC 3 cut(s) 112, 313, 367
Sau96I GGNCC 1 cut(s) 562
ScrFI CCNGG 1 cut(s) 330
SduI GDGCHC 1 cut(s) 286
SfaNI GCATC 2 cut(s) 255, 490
Sfr303I CCGCGG 1 cut(s) 568
SgrBI CCGCGG 1 cut(s) 568
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 5 cut(s) 29, 140, 151, 411, 557
SseBI AGGCCT 1 cut(s) 274
SsiI CCGC 2 cut(s) 565, 567
SspMI CTAG 1 cut(s) 552
StuI AGGCCT 1 cut(s) 274
StyD4I CCNGG 1 cut(s) 328
TaaI ACNGT 4 cut(s) 21, 55, 180, 345
TaiI ACGT 2 cut(s) 159, 174
TaqI TCGA 1 cut(s) 92
TasI AATT 5 cut(s) 29, 140, 151, 411, 557
TatI WGTACW 1 cut(s) 436
TfiI GAWTC 3 cut(s) 89, 161, 488
TscAI CASTG 1 cut(s) 185
TspDTI ATGAA 4 cut(s) 102, 241, 404, 474
TspRI CASTG 1 cut(s) 185
XapI RAATTY 2 cut(s) 29, 411
XmnI GAANNNNTTC 1 cut(s) 248
XspI CTAG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.