Rh4DG044600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008178
Physical Location & Seq
Reverse (-)
7292450 .. 7323526
31077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG044600.1

Sequence Viewer

Length: 561 bp
ATGGAGGAATTAAGGGAGACTTCGTCTCTCCTGCTAGATGATTTCGAAGAAAACCTGCAGTCTATCACCTTCGAGTCTCAGCATTGGTGGCGGTCCAGTTTCGCCAGCGAGAGTAGGAAAGGTTGCAGGAAGATTCGATGCAGTTACTCACATGAAGTATACAGAATTTGCTGGGGCAGTTCCTATTCAGTTTCAAGGTTTATACAAAATGGTGGTTCTTTTGCCAACTACTACATGTATCTCAACAAGACCCGGTGGAAGAGGAAGAGATTGTGCAGATCAACAATGAAGAAATCGATTGGAAAGGTTGCAAGAATATATGGCAAGCTCCAACTTGTACGGCTTGGTAGGTCTGCGGGAACCACATGGCAAAACAACAACCCAACTGCAGAGAAGCCAACAGCGCCGTCAGGAGGCTACTCCTCCTCGTCGGCAGCGTCAGAGCCAACGCCGGCGCCTGCCGCTTCAGCCTCGACCGCCGCAGCAGACGGGTCTCCTACTAAGCGTGGTGAAACTGCGGCAAAGAAGTCGCGTCAGCATACCCGAGTAGCCGGCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

20.69

Weight (kDa)

10.05

Isoelectric Point (pI)

57.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 63
AccB1I GGYRCC 2 cut(s) 454, 554
AccI GTMKAC 1 cut(s) 159
AccII CGCG 1 cut(s) 532
AciI CCGC 6 cut(s) 91, 356, 462, 477, 480, 518
AcsI RAATTY 1 cut(s) 165
AcuI CTGAAG 1 cut(s) 450
AcyI GRCGYC 2 cut(s) 455, 555
AfaI GTAC 1 cut(s) 339
AfiI CCNNNNNNNGG 1 cut(s) 413
AflIII ACRYGT 1 cut(s) 234
AgsI TTSAA 1 cut(s) 195
AluBI AGCT 1 cut(s) 328
AluI AGCT 1 cut(s) 328
Alw26I GTCTC 4 cut(s) 11, 30, 81, 498
Ama87I CYCGRG 1 cut(s) 543
ApeKI GCWGC 2 cut(s) 434, 482
ApoI RAATTY 1 cut(s) 165
AspLEI GCGC 3 cut(s) 406, 457, 557
AspS9I GGNCC 1 cut(s) 93
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 2 cut(s) 58, 521
AsuII TTCGAA 1 cut(s) 45
AvaI CYCGRG 1 cut(s) 543
AvaII GGWCC 1 cut(s) 93
BanI GGYRCC 2 cut(s) 454, 554
BbvI GCAGC 2 cut(s) 446, 494
BceAI ACGGC 2 cut(s) 356, 391
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 4 cut(s) 11, 30, 81, 498
BfaI CTAG 2 cut(s) 35, 559
BfmI CTRYAG 2 cut(s) 56, 387
BfoI RGCGCY 3 cut(s) 407, 458, 558
BfuAI ACCTGC 1 cut(s) 63
BisI GCNGC 5 cut(s) 435, 462, 480, 483, 519
BlsI GCNGC 5 cut(s) 436, 463, 481, 484, 520
Bme1390I CCNGG 1 cut(s) 253
Bme18I GGWCC 1 cut(s) 93
BmeT110I CYCGRG 1 cut(s) 543
BmgT120I GGNCC 1 cut(s) 93
BmiI GGNNCC 3 cut(s) 361, 456, 556
BmrFI CCNGG 1 cut(s) 253
BmsI GCATC 1 cut(s) 128
Bpu14I TTCGAA 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 253
Bsa29I ATCGAT 1 cut(s) 296
BsaHI GRCGYC 2 cut(s) 455, 555
BsaI GGTCTC 1 cut(s) 498
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 1 cut(s) 413
Bse118I RCCGGY 2 cut(s) 451, 551
Bse1I ACTGG 1 cut(s) 96
BseCI ATCGAT 1 cut(s) 296
BseLI CCNNNNNNNGG 1 cut(s) 413
BseMII CTCAG 1 cut(s) 92
BseNI ACTGG 1 cut(s) 96
BseRI GAGGAG 2 cut(s) 412, 415
BseXI GCAGC 2 cut(s) 446, 494
BseYI CCCAGC 1 cut(s) 171
BsgI GTGCAG 1 cut(s) 295
Bsh1236I CGCG 1 cut(s) 532
Bsh1285I CGRYCG 1 cut(s) 477
BshNI GGYRCC 2 cut(s) 454, 554
BshVI ATCGAT 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 477
BsiHKCI CYCGRG 1 cut(s) 543
BsiSI CCGG 3 cut(s) 253, 452, 552
BslI CCNNNNNNNGG 1 cut(s) 413
BsmAI GTCTC 4 cut(s) 11, 30, 81, 498
BsmBI CGTCTC 1 cut(s) 30
Bso31I GGTCTC 1 cut(s) 498
BsoBI CYCGRG 1 cut(s) 543
Bsp119I TTCGAA 1 cut(s) 45
Bsp143I GATC 1 cut(s) 278
BspACI CCGC 6 cut(s) 91, 356, 462, 477, 480, 518
BspCNI CTCAG 1 cut(s) 91
BspDI ATCGAT 1 cut(s) 296
BspFNI CGCG 1 cut(s) 532
BspLI GGNNCC 3 cut(s) 361, 456, 556
BspMAI CTGCAG 2 cut(s) 60, 391
BspMI ACCTGC 1 cut(s) 63
BspT104I TTCGAA 1 cut(s) 45
BspT107I GGYRCC 2 cut(s) 454, 554
BspTNI GGTCTC 1 cut(s) 498
BsrFI RCCGGY 2 cut(s) 451, 551
BsrI ACTGG 1 cut(s) 96
BssAI RCCGGY 2 cut(s) 451, 551
BssMI GATC 1 cut(s) 278
BssNAI GTATAC 1 cut(s) 160
BssNI GRCGYC 2 cut(s) 455, 555
Bst1107I GTATAC 1 cut(s) 160
Bst6I CTCTTC 2 cut(s) 254, 260
BstACI GRCGYC 2 cut(s) 455, 555
BstBI TTCGAA 1 cut(s) 45
BstC8I GCNNGC 5 cut(s) 106, 326, 453, 459, 553
BstDEI CTNAG 2 cut(s) 78, 501
BstFNI CGCG 1 cut(s) 532
BstH2I RGCGCY 3 cut(s) 407, 458, 558
BstHHI GCGC 3 cut(s) 406, 457, 557
BstKTI GATC 1 cut(s) 281
BstMAI GTCTC 4 cut(s) 11, 30, 81, 498
BstMBI GATC 1 cut(s) 278
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 5 cut(s) 88, 403, 461, 467, 476
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 251
BstSFI CTRYAG 2 cut(s) 56, 387
BstUI CGCG 1 cut(s) 532
BstV1I GCAGC 2 cut(s) 446, 494
BstZ17I GTATAC 1 cut(s) 160
Bsu15I ATCGAT 1 cut(s) 296
BsuTUI ATCGAT 1 cut(s) 296
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 5 cut(s) 106, 326, 453, 459, 553
CfoI GCGC 3 cut(s) 406, 457, 557
Cfr10I RCCGGY 2 cut(s) 451, 551
Cfr13I GGNCC 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 296
CseI GACGC 2 cut(s) 426, 521
Csp6I GTAC 1 cut(s) 338
CviAII CATG 3 cut(s) 152, 235, 366
CviJI RGCY 7 cut(s) 328, 343, 397, 417, 445, 470, 551
CviKI_1 RGCY 7 cut(s) 328, 343, 397, 417, 445, 470, 551
CviQI GTAC 1 cut(s) 338
DdeI CTNAG 2 cut(s) 78, 501
DinI GGCGCC 2 cut(s) 456, 556
DpnI GATC 1 cut(s) 280
DpnII GATC 1 cut(s) 278
Eam1104I CTCTTC 2 cut(s) 254, 260
EarI CTCTTC 2 cut(s) 254, 260
Eco31I GGTCTC 1 cut(s) 498
Eco47I GGWCC 1 cut(s) 93
Eco57I CTGAAG 1 cut(s) 450
Eco88I CYCGRG 1 cut(s) 543
EgeI GGCGCC 2 cut(s) 456, 556
EheI GGCGCC 2 cut(s) 456, 556
Esp3I CGTCTC 1 cut(s) 30
FaeI CATG 3 cut(s) 155, 238, 369
FaiI YATR 8 cut(s) 153, 160, 203, 236, 319, 321, 367, 540
FalI AAGNNNNNCTT 1 cut(s) 36
FatI CATG 3 cut(s) 151, 234, 365
FauI CCCGC 1 cut(s) 349
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 5 cut(s) 435, 462, 480, 483, 519
Fsp4HI GCNGC 5 cut(s) 435, 462, 480, 483, 519
FspBI CTAG 2 cut(s) 35, 559
GlaI GCGC 3 cut(s) 405, 456, 556
GluI GCNGC 5 cut(s) 435, 462, 480, 483, 519
GsaI CCCAGC 1 cut(s) 175
HaeII RGCGCY 3 cut(s) 407, 458, 558
HapII CCGG 3 cut(s) 253, 452, 552
HgaI GACGC 2 cut(s) 426, 521
HhaI GCGC 3 cut(s) 406, 457, 557
Hin1I GRCGYC 2 cut(s) 455, 555
Hin1II CATG 3 cut(s) 155, 238, 369
Hin6I GCGC 3 cut(s) 404, 455, 555
HinP1I GCGC 3 cut(s) 404, 455, 555
HinfI GANTC 2 cut(s) 74, 133
HpaII CCGG 3 cut(s) 253, 452, 552
HphI GGTGA 2 cut(s) 58, 521
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 1 cut(s) 411
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 433
HpyAV CCTTC 1 cut(s) 79
HpyCH4V TGCA 6 cut(s) 58, 126, 141, 276, 311, 389
HpyF10VI GCNNNNNNNGC 5 cut(s) 88, 403, 461, 467, 476
HpyF3I CTNAG 2 cut(s) 78, 501
Hsp92I GRCGYC 2 cut(s) 455, 555
Hsp92II CATG 3 cut(s) 155, 238, 369
HspAI GCGC 3 cut(s) 404, 455, 555
KasI GGCGCC 2 cut(s) 454, 554
KroI GCCGGC 2 cut(s) 451, 551
KroNI GCCGGC 2 cut(s) 453, 553
Kzo9I GATC 1 cut(s) 278
LmnI GCTCC 1 cut(s) 333
Lsp1109I GCAGC 2 cut(s) 446, 494
LweI GCATC 1 cut(s) 128
MaeI CTAG 2 cut(s) 35, 559
MaeIII GTNAC 1 cut(s) 143
MalI GATC 1 cut(s) 280
MboI GATC 1 cut(s) 278
MboII GAAGA 5 cut(s) 59, 142, 271, 277, 301
MluCI AATT 2 cut(s) 8, 165
Mly113I GGCGCC 2 cut(s) 455, 555
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 355
MnlI CCTC 5 cut(s) 255, 407, 433, 436, 481
MreI CGCCGGCG 1 cut(s) 451
MroNI GCCGGC 2 cut(s) 451, 551
MseI TTAA 1 cut(s) 11
MspI CCGG 3 cut(s) 253, 452, 552
MspR9I CCNGG 1 cut(s) 253
MvnI CGCG 1 cut(s) 532
MwoI GCNNNNNNNGC 5 cut(s) 88, 403, 461, 467, 476
NaeI GCCGGC 2 cut(s) 453, 553
NarI GGCGCC 2 cut(s) 455, 555
NciI CCSGG 1 cut(s) 253
NdeII GATC 1 cut(s) 278
NgoMIV GCCGGC 2 cut(s) 451, 551
NlaIII CATG 3 cut(s) 155, 238, 369
NlaIV GGNNCC 3 cut(s) 361, 456, 556
NspI RCATGY 1 cut(s) 238
NspV TTCGAA 1 cut(s) 45
PciI ACATGT 1 cut(s) 234
PcsI WCGNNNNNNNCGW 1 cut(s) 434
PdiI GCCGGC 2 cut(s) 453, 553
PfeI GAWTC 1 cut(s) 133
PflFI GACNNNGTC 1 cut(s) 22
PkrI GCNGC 5 cut(s) 436, 463, 481, 484, 520
PleI GAGTC 1 cut(s) 82
PluTI GGCGCC 2 cut(s) 458, 558
PpsI GAGTC 1 cut(s) 82
PscI ACATGT 1 cut(s) 234
PspFI CCCAGC 1 cut(s) 171
PspN4I GGNNCC 3 cut(s) 361, 456, 556
PspPI GGNCC 1 cut(s) 93
PstI CTGCAG 2 cut(s) 60, 391
PsyI GACNNNGTC 1 cut(s) 22
RsaI GTAC 1 cut(s) 339
RsaNI GTAC 1 cut(s) 338
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 5 cut(s) 435, 462, 480, 483, 519
Sau3AI GATC 1 cut(s) 278
Sau96I GGNCC 1 cut(s) 93
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 1 cut(s) 253
SetI ASST 7 cut(s) 57, 71, 124, 200, 309, 330, 353
SfaNI GCATC 1 cut(s) 128
SfcI CTRYAG 2 cut(s) 56, 387
SfoI GGCGCC 2 cut(s) 456, 556
SfuI TTCGAA 1 cut(s) 45
SgrAI CRCCGGYG 1 cut(s) 451
SinI GGWCC 1 cut(s) 93
Sse9I AATT 2 cut(s) 8, 165
SsiI CCGC 6 cut(s) 91, 356, 462, 477, 480, 518
SspDI GGCGCC 2 cut(s) 454, 554
SspMI CTAG 2 cut(s) 35, 559
StyD4I CCNGG 1 cut(s) 251
TaqI TCGA 5 cut(s) 45, 72, 136, 296, 473
TasI AATT 2 cut(s) 8, 165
TauI GCSGC 3 cut(s) 464, 482, 521
TfiI GAWTC 1 cut(s) 133
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TseI GCWGC 2 cut(s) 434, 482
TspDTI ATGAA 2 cut(s) 168, 302
Tth111I GACNNNGTC 1 cut(s) 22
VpaK11BI GGWCC 1 cut(s) 93
XapI RAATTY 1 cut(s) 165
XceI RCATGY 1 cut(s) 238
XmiI GTMKAC 1 cut(s) 159
XspI CTAG 2 cut(s) 35, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.